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Citations for Beckman :
201 - 250 of 379 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates were subjected to two rounds of centrifugation at 18000 rpm for 5 and 30 mins in a JA-25.50 rotor (Beckman). Protein levels were normalized for coimmunoprecipitation and immunoblot analysis using a Bradford Assay ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... containing cell nucleus and other heavy organelles was discarded and supernatant was centrifuged for 30 min at 48,000 × g at 4 °C (Beckman Avanti J-251 Ultra-centrifuge ...
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bioRxiv - Biophysics 2024Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman), before being incubated overnight at 4°C with 1.5 mL of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... Media collected from the models after one week in culture was subjected to centrifugation twice at 10,000g for 30 min before being centrifuged at 100,000g with tabletop ultracentrifuge (Beckman Coulter) for 70 min at 4 °C.
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bioRxiv - Biochemistry 2024Quote: ... Bio-beads SM2 were then removed and the reconstitution mixture was cleared by centrifugation at 86,600 g for 30 min in the TLA 100.3 rotor (Beckman Coulter).
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by ultracentrifugation at 30,000 × g 30 min at 4°C using a Ti45 rotor (Beckman Coulter) twice ...
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bioRxiv - Microbiology 2020Quote: ... and overlaid with 6 ml of 30% and 3 ml of 5% sucrose (w/v) followed by centrifugation at 32,000 rpm in SW41 rotor (Beckman Coulter) for 20 h ...
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bioRxiv - Immunology 2021Quote: ... Full-length double-stranded cDNA was purified with 30 μl (0.6x) of AMPure XP magnetic beads (Beckman Coulter, Cat. #A63881) and eluted in 20 μl of water ...
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bioRxiv - Microbiology 2021Quote: ... the suspension was stored overnight at 30°C and peptidoglycan was collected by centrifugation at 48,000 rpm in SW 55 Ti rotor (Beckman Coulter) for 2 h at 30°C ...
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bioRxiv - Microbiology 2021Quote: ... Clarified supernatant was then layered on a 30% sucrose cushion and ultra-centrifuged at 30,000 RPM for 2.5 hours (Beckman Rotor SW41). Pelleted virus was resuspended in PBS and TRIzol (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: Pooled seminal plasma from stallions was centrifuged for 30 min at 10,000g and 4°C (Beckman Coulter, BC - rotor SW32Ti) to pellet cell debris and larger molecular complexes ...
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bioRxiv - Biophysics 2021Quote: ... The polymerized microtubules were diluted in BRB80T (BRB80 supplemented with 10μM taxol (paclitaxel)) and centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter). After centrifugation the pellet was resuspended in BRB80T.
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bioRxiv - Cell Biology 2021Quote: ... The cell lysate was clarified via centrifugation at 66,000 x g for 30 min in a Type 70 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... for 5 minutes using a FIBERLite F10BCI-6×500y rotor (Piramoon Technologies) in an Avanti JXN-30 centrifuge (Beckman Coulter). The clarified cell lysate was further centrifuged in a JS-24.38 rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... Virus was pelleted at 1847 x g for 30 min at 4°C in the plate in an Allegra centrifuge (Beckman) and the pellet was resuspended in 50 uL of loading buffer with DTT for analysis by WB with αGag ...
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bioRxiv - Biophysics 2022Quote: ... the homogenized solution was brought to 55 mL with more Percoll buffer and centrifuged at 48400 g for 30 minutes in a 45 Ti rotor (Beckman). Chromosomes appeared as a faint band about 1/5th from the bottom of the centrifuge tube ...
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bioRxiv - Microbiology 2022Quote: ... The capsid-host protein complexes were sedimented through a 30% sucrose cushion at 110,000 g for 20 min at 4°C (TLA-100, Beckman-Coulter), resuspended in CBB by ultrasound tip sonication at 40 W for about 5 x 5 seconds on ice ...
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bioRxiv - Biophysics 2022Quote: ... The virion was sedimented twice through a 30% sucrose band to 50% band in PBS buffer by ultracentrifugation (Beckman, IN) at 100,000 g ...
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bioRxiv - Cell Biology 2022Quote: ... a range of concentrations of Crn1 protein variants was incubated in F-buffer with pre-polymerized actin filaments at room temperature for 30 min and then spun at 100,000 × g (high speed) with TLA100 rotor (Beckman Coulter) for 20 min at 25°C ...
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bioRxiv - Microbiology 2020Quote: ... Cell containing supernatant was recovered into additional falcon tubes and centrifuged at 10,000G for 20 minutes with 4ºC cooling to pellet cells using an Avanti JXN-30 highspeed centrifuge with JA 14.50 fixed angle rotor (Beckman Coulter). Supernatant was removed without disturbing the cell pellet ...
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bioRxiv - Molecular Biology 2020Quote: ... and the cleared supernatant was loaded on a 3 to 30% iodixanol gradient (OptiPrep) and ultracentrifuged for 5h at 36,000 rpm in an SW60 rotor (Beckman Coulter). Fractions were collected manually through aspiration and RNA or proteins were isolated for further analysis.
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bioRxiv - Biochemistry 2021Quote: ... The sucrose loaded heavy MLVs were centrifuged at 180,000 x g for 30 min at 4°C using Optima TL benchtop Ultracentrifuge (Beckman Coulter). The resulting pellets were buffer exchanged by resuspension into the assay buffer comprising 50 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... the homogenate was layered atop a 30%-40% iodixanol gradient and centrifuged in an SW-41 swing bucket rotor (Beckman) at 10,000g for 18 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Insoluble material was removed by centrifugation (100,000 x g; 30 min; 4°C; 45 Ti rotor; Optima XPN-80; Beckman Coulter). The cell lysate supernatant was passed through a Ni+2-NTA resin column (Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane fraction was pelleted down by high-speed centrifugation (100,000 x g; 4°C; 30 min; 45Ti rotor; Optima XPN-80, Beckman Coulter). The membrane pellet was resuspended in solubilization buffer ...
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bioRxiv - Plant Biology 2020Quote: ... The insoluble cell debris was removed via centrifugation for 30 min at 4°C and 20,000 rpm (JA-25.50, Beckman Coulter®). The supernatant was incubated for 1 h with low agitation and with 1.2 ml Glutathione-sepharose (GE-Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... the cells were harvested by centrifuging for 30 min at 4°C and 6,000 rpm (JA-10, Beckman Coulter®). The cells were resuspended in 1x PBS (pH 7.3 ...
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bioRxiv - Microbiology 2020Quote: ... parkeri was overlaid on a 30% MD-76R (Merry X-Ray) gradient solution in ultracentrifuge tubes (Beckman/Coulter Cat 344058). Gradients were centrifuged at 18,000 r.p.m ...
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bioRxiv - Microbiology 2020Quote: ... through a 1 ml 30% sucrose cushion (in phosphate-buffered saline, PBS, pH 7.4) in a SW41 rotor (Beckman coulter). The pelleted virus material was re-solubilized in PBS and analyzed by sodium docecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with following antibody mix for 30 minutes on ice: pan-γδTCR-PE (1:10; IMMU510; Beckman Coultier) and CD69-APC (1:25 ...
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bioRxiv - Immunology 2021Quote: ... The supernatant of the cultures was then collected and centrifuged at 16,000g for 30 min (J6-MI Centrifuge – Beckman Coulter). Finally ...
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bioRxiv - Genetics 2023Quote: ... Sub-reactions were mixed and purification was performed using 30 μL AMPure XP magnetic beads (Beckman Coulter; cat. no. A63880) according to manufacturer’s instructions and eluted in 21 μL H2O ...
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bioRxiv - Neuroscience 2023Quote: ... The resultant supernatant was centrifuged at 200,000 × g for 30 min to obtain the LP2 fraction using a TL100 ultracentrifuge (Beckman Coulter). Both LP1 and LP2 pellets were resuspended in Tris-HCl buffer containing 1mM EDTA ...
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bioRxiv - Microbiology 2023Quote: ... Proteoliposomes were then concentrated by ultracentrifugation (30 min; 100.000 g; 12 ºC) in an Optima Max XP Tabletop Ultracentrifuge (Beckman Coulter), resuspended in Internal Buffer and kept at 12 ºC.
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bioRxiv - Cancer Biology 2023Quote: ... clarified by centrifugation at 20,000 rpm (48,385 x g) for 30 min in a JA25.50 rotor at 4 °C (Beckman AvantiTM J-25), filtered through a 0.8/0.2 AcroPak Filter Unit (Pall) ...
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bioRxiv - Molecular Biology 2023Quote: ... then pelleted at 4°C and 4,300 x g for 30 min in an Allegra X-14R centrifuge (Beckman Coulter) equipped with a swinging bucket SX4750 rotor ...
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bioRxiv - Cancer Biology 2023Quote: ... Large EVs were collected by centrifugation at 10,000 x g for 30 minutes in a Ti 45 rotor (Beckman Coulter). Small EVs were collected by a modified cushion-density gradient protocol (Li et al. ...
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bioRxiv - Systems Biology 2023Quote: ... The urine was centrifuged at 20,000 x g for 30 minutes at 4°C (k-factor 1,790) in an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW32 Ti swinging bucket rotor (Rmin 67 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by sonication and the lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The lysate was passed three times though 2mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by sonication and the lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The lysate was passed three times through 2 mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... the post-nuclear supernatant was centrifuged for 30 min at 100,000 x g at 4°C using a S120AT2 rotor (Beckman Coulter). The resulting pellet containing the membranes was resuspended in SDS sample buffer (see below).
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bioRxiv - Microbiology 2023Quote: ... A volume of 30 mL of supernatant was dispensed in two 38.5 mL ultracentrifuge tubes (Ultra-Clear, 25×89 mm, 344058, Beckman Coulter) before adding 5 mL of a 20% sucrose solution in NTE buffer (100 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... then at 25°C for 1 hour before spinning at 300,000 g for 30 min at 25 °C in a TLA100 rotor in an Optima MAX-TL ultracentrifuge (Beckman Coulter). Supernatants were separated from pellets ...
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bioRxiv - Biophysics 2024Quote: ... The induced culture was then harvested by centrifuging at 3500 rpm for 30 min in a Beckman Coulter Avanti J-25 ultracentrifuge using a JLA-8.1 rotor (Beckman Coulter). The pelleted cells were resuspended in 150 ml of Lysis buffer (50 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The homogenate was clarified by centrifugation for 30 min at 70,560 × g at 4°C in an Optima XPN Ultracentrifuge (Beckman Coulter) using a Ti-45 rotor ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 minutes at 37 °C prior to centrifugation at 18.000 x g for 30 min in a Microfuge 18 Centrifuge (Beckman Coulter) and resuspension of the microtubule pellet in BRB80 supplemented with 10 µM Taxol (Sigma Aldrich ...
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bioRxiv - Immunology 2023Quote: ... virus solutions were clarified of debris by centrifugation at 10,000 rpm at 4°C for 30 minutes in a chilled SW32Ti rotor (Beckman Coulter), followed by pelleting through a 30% cold sucrose solution at 25,000 rpm for 90 minutes ...
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bioRxiv - Immunology 2023Quote: ... This clear media supernatant was loaded on top of 30% sucrose gradient and was followed by ultracentrifugation at 100,000xg for 90mins on SW41Ti (Beckman Coulter) rotor ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were harvested at 6000 rpm for 30 min in a JLA 9.1000 rotor (Beckman Coulter, Brea, California, USA), washed with 150 mM NaCl ...