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Citations for Beckman :
301 - 350 of 379 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... viruses were collected and concentrated by ultracentrifugation (37,000 rpm, 2 h 30, 15 °C; Beckman Type 45 Ti fixed-angle rotor). The virus titer was determined using a plaque assay.
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bioRxiv - Cancer Biology 2023Quote: ... media was changed at 6hrs and 72hrs supernatants were collected and concentrated using ultracentrifugation above a 30% sucrose cushion for 2hrs at 25,000 rpm (Beckman; SW32-Ti). Supernatants were discarded and pelleted lentivirus was re-suspended overnight at 4C in 200 µL of PBS ...
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bioRxiv - Biochemistry 2023Quote: ... and the lysates were centrifuged at 100,000 ×g for 30 min in a Beckman 70.1Ti rotor (Beckman Coulter, CA, USA, #342184). The supernatant was loaded onto a DEAE Sepharose Fast Flow column (Cytiva ...
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bioRxiv - Systems Biology 2023Quote: ... We performed double-sided library purifications on the pooled samples using first 0.6x (30 μL x the number of pooled reactions) AMPure XP magnetic beads (Beckman Coulter, A63881) and keeping the supernatant ...
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bioRxiv - Cell Biology 2023Quote: ... Pre-enriched preparations of shed red bodies suspended in water were applied to the top of the gradient and centrifuged for 30 min at 40,000 rcf in an Optima XE-90 Ultracentrifuge (Beckman Coulter, USA) with SW60 Ti rotor and Beckman Ultra-Clear 11 x 60 mm centrifuge tubes (#344062).
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bioRxiv - Microbiology 2023Quote: ... After six days of photoautotrophic growth the culture was centrifuged for 30 minutes at 4,500 rpm to concentrate the biomass (Allegra X-22R, Beckman Coulter, USA). The wet biomass was then divided into 20 mL serum bottles sealed with butyl-rubber septa ...
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bioRxiv - Molecular Biology 2023Quote: ... Phage particles in the supernatant were precipitated by ultracentrifugation at 100,000 rpm for 30 min using a TLS-110 rotor (Optima TLX; Beckman Coulter Inc.). The resulting pellet was suspended in 800 μL of Tris-EDTA solution (pH 8.0) ...
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bioRxiv - Molecular Biology 2023Quote: ... A 10-30% gradient of glycerol in HEMG buffer was poured into 14 x 89 mm polyallomer centrifuge tubes (Beckman #331372). The resuspended nuclear extract was carefully layered on top of the gradient and centrifuged in an SW41 rotor at 40,000 rpm for 16 hours at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant was then centrifugated at 10,000×g for 30 min followed by ultracentrifugation at 100,000×g (Optima XE-100 Ultracentrifuge, Beckman Coulter, Brea, CA) for 2 h ...
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bioRxiv - Cell Biology 2023Quote: ... 30% Percoll (v/v)] followed by 3 mL of MRB in a 13 mL ultracentrifuge tube (Beckman Instruments, Inc, CA 331372) and spun at 95,000 x g for 45 min in a TH641 rotor ...
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bioRxiv - Immunology 2024Quote: ... Centrifugation was performed (30 min, 800 g, 4 °C) in a swinging bucket on a Beckman Coulter JS-13.1 rotor (Beckman Coulter, USA). Hemocytes concentrated at each density interface were collected separately with a 70 mm long 20Gx2.75” needle mounted on a 1 mL syringe ...
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bioRxiv - Molecular Biology 2024Quote: ... Sulfo-Cy5 at a final concentration of 1 µM was then mixed with the cells for 30 minutes at 37 °C before the cells were trypsinzed and analyzed by using flow cytometry (CytoFLEX, Beckman Coulter).
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated for 30 minutes at room temperature in the dark and analyzed on a Gallios flow cytometer (Beckman Coulter) for DNA content ...
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bioRxiv - Biophysics 2021Quote: ... Frozen aliquots were thawed and centrifuged for 30 min at 120,000 g in a benchtop Beckman air-driven ultracentrifuge (Beckman Coulter Airfuge, 340401) to clear the solution from aggregates ...
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bioRxiv - Biochemistry 2021Quote: ... Binding reactions were spun at 300,000 g for 30 min at 25 °C using Optima MAX-TL ultracentrifuge (Beckman Coulter, Brea, CA). Bundling reactions were spun at 17,000 g for 15 min at 25 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Frozen aliquots were thawed and centrifuged for 30 min at 120,000 g in a benchtop Beckman air-driven ultracentrifuge (Beckman Coulter Airfuge, 340401) to clear the solution from aggregates ...
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bioRxiv - Neuroscience 2020Quote: ... The lysate was cleared by centrifugation at 30,000 g for 30 minutes in a JA 25.50 rotor (Beckman Coulter, Brea, CA, USA). Streptomycin sulfate was added to the lysate at 10 mg/ml to precipitate DNA ...
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bioRxiv - Cell Biology 2021Quote: ... Virus was concentrated from cell-free culture supernatant by high-speed ultracentrifugation through a 30% sucrose cushion at 80,000xg for 16 hrs in a SW32 rotor (k-factor 321) (Beckman Coulter, Brea, CA).
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bioRxiv - Cell Biology 2021Quote: ... Frozen aliquots were thawed and centrifuged for 30 min at 120,000 g in a benchtop Beckman air-driven ultracentrifuge (Beckman Coulter Airfuge, 340401) to clear the solution from aggregates ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was then passed through a cell sieve and underwent a 30 min ultracentrifugation (70 Ti rotor, Beckman Coulter, CA, USA) at 10,000 x g ...
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bioRxiv - Molecular Biology 2022Quote: ... were pre-clarified to remove aggregate proteins by spinning at 65,000 rpm for 30 min at 4°C in a TLA100 rotor (Beckman Coulter, Brea, CA) before incubating with F-actin ...
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bioRxiv - Biophysics 2020Quote: ... cell lysate was centrifuged by using The Beckman Optima™ L-80 XP Ultracentrifuge at 40000 rpm for 30 minutes at 4°C by using Ti45 rotor (Beckman, USA). After ultracentrifugation the pellet which contains membranes and insoluble debris was discarded and clear supernatant applied to nickel affinity chromatography by using a Ni-NTA agarose resin (QIAGEN ...
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bioRxiv - Physiology 2022Quote: ... The amplified cDNA (a total of 30-50 μL) was purified using 90 μL Beckman Coulter AMPure XP beads (Beckman Coulter, #A63880) following the manufacturer’s guideline ...
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bioRxiv - Biochemistry 2020Quote: ... disrupted with a tip sonicator at 1/3 power (8 MHz) for 3 × 30 seconds on ice and fractionated by ultracentrifugation (100’000 x g, 1H, Beckman TLA 120.2 rotor). The luminal fraction was concentrated to around 50 µL on a 3 kDa MWCO Amicon Ultra 0.5 mL centrifugal filter while membrane pellets were dissolved in 50 µL TBS with 2% SDS ...
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bioRxiv - Microbiology 2020Quote: ... Diluted virions were then centrifuged at 75,000 RPM for 30 minutes at 11 °C in a Beckman TLA 120 rotor (Beckman Coulter, Brea, California). The pellet was resuspended in 10 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... The lysate was cleared by centrifugation at 30,000 g for 30 minutes in an JA 25.50 rotor (Beckman Coulter, Brea, CA, USA). The His-tagged target protein was purified by Ni-NTA gravity-flow chromatography (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... supernatants were centrifuged at 10,000 x g for 30 min at 4°C and the supernatants were transferred to ultracentrifuge tubes (Beckman Coulter, Brea, CA) and diluted with phosphate-buffered saline (PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were layered on top of 1 ml of 0.85 M sucrose in 2 mM EGTA and centrifuged at 75,000 g for 30 min in a Beckman FW-27 rotor (Beckman, Fullerton, CA, USA). This step was repeat twice ...
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bioRxiv - Genomics 2021Quote: ... The 30 libraries were pooled and purified with a double-sided size-selection using SPRIselect paramagnetic beads (Beckman Coulter Life Sciences, B23318), checked for concentration and fragment size ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatants were further centrifuged at 10,000 × g for 30 min at 4°C to remove remaining cell debris and at 100,000 × g (Type 90 Ti Rotor, Beckman, Brea, CA, USA) overnight at 4°C to remove exosomes ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was collected and virus pelleted through 30% (w/v) sucrose cushion at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 hours at 10°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The plates were then centrifuged at 2000 rpm (931 x g) for 2h at 30°C (Allegra® X-15R Centrifuge (Beckman Coulter), rotor SX4750A) ...
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bioRxiv - Cell Biology 2023Quote: ... Gradients were then chilled on ice for 30 minutes prior to loading lysate and centrifugation at 40,000 rpm on a TLS-55 swinging bucket rotor (Beckman Coulter, Brea, CA) for 16 hours at 4°C ...
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HIV-2 Immature Particle Morphology Provides Insights into Gag Lattice Stability and Virus MaturationbioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in STE buffer and banded out over an OptiPrepTM step gradient (10, 20, 30% OptiPrepTM (v/v)) with a SW55 Ti rotor (Beckman Coulter, CA) at 45,000 rpm for 3 h ...
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bioRxiv - Neuroscience 2023Quote: ... then spun at 100,000 x g for 30 min at 4°C in an ultracentrifuge (Beckman OPTIMA TLX with TLA-100.3 rotor). The supernatant was saved and the pellet went through a series of similar extractions using High-Salt Triton Buffer (High-Salt Buffer ...
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bioRxiv - Cell Biology 2023Quote: ... cells were blocked in 2% human serum and PBS for 30 min at 4°C and stained with DC-SIGN (Beckman Coulter #A07407), CD80 (BioLegend #305214) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated at RT for 30 minutes and centrifugated at 20,000 x g for 10 minutes (Avanti J-30I Centrifuge, Beckman Coulter, Brea, CA). The supernatant was collected and diluted 1:5 with incubation buffer (final concentration ∼204 cells mL-1) ...
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bioRxiv - Biochemistry 2023Quote: ... by centrifugation at 80,000 × g for 30 min at 4°C using a TLS-55 rotor (Beckman Coulter Inc., Miami, FL, USA). Crista membranes were identified and removed based on fluorescence under a UV lamp ...
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bioRxiv - Developmental Biology 2024Quote: ... for 30 min at 4°C in the dark followed by flow cytometry analysis on a CytoFlex 26 (Beckman Coulter, Indianapolis, IN) in PE-A and PerCP-A channels ...
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bioRxiv - Microbiology 2024Quote: ... The sample was then vortexed and ultracentrifuged at 50,000 x g for 30 minutes at 20°C (Beckman Coulter Type 45 Ti). Proteinase K (200 μg/ml ...
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bioRxiv - Microbiology 2024Quote: ... which contained bacteria, was then overlayed on 30% RenoCal-76 (diluted in K-36 buffer; Bracco Diagnostics, 04H208) in ultracentrifuge tubes (Beckman/Coulter, 344058). Tubes were then placed in a SW-32TI rotor (Beckman/Coulter ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting supernatant was centrifuged at 200,000 x g for 30 minutes at 4 °C using a Beckman Optima Ultracentrifuge with TLA110 rotor (Beckman Coulter Life Sciences). The pellet was resuspended in 30 µL of cold ddH20 and stored in −80 °C until further use.
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bioRxiv - Neuroscience 2024Quote: ... The suspension was then centrifuged at 2500 × g for 30 min at 4 °C and the supernatant was transferred to an ultra-centrifugation tube (Quickseal Tubes, Beckman Coulter #342414). AAV purification was performed by ultra-centrifugation over a discontinuous Iodixanol density gradient (OptiPrep Density Gradient Medium ...
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bioRxiv - Neuroscience 2024Quote: ... This gradient was centrifuged for 30 minutes at 72,000 x g at 4°C (33,000 rpm, TLS-55 rotor, Beckman Coulter Optima TLX Ultracentrifuge). After centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were harvested at 7 400 x g for 30 min in a JLA 9.1000 rotor (Beckman Coulter, Brea, California, USA), washed with 150 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... and the sample was incubated at 25°C for 30 min and then moved to a polycarbonate ultracentrifuge tube (Beckman Coulter, 362305). A 3 ml sucrose cushion (10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2024Quote: Cells from frozen stocks were diluted 1:30 into 300 µl of LB in a deep 96-well plate (Beckman Coulter, #267007), covered with a breathable film ...
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bioRxiv - Genetics 2024Quote: ... Samples were allowed to warm to room temperature for 30 minutes and then were incubated with 65 µL SPRIselect beads (Beckman Coulter, B23318) (1.3X ...
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bioRxiv - Biophysics 2022Quote: ... shortly sonified and centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1).) ...
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bioRxiv - Biophysics 2022Quote: ... The samples were centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1). The supernatant ...