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Citations for Beckman :
101 - 150 of 379 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified by centrifugation at 35 Krpm for 30 min in a 45ti rotor (Beckman). The supernatant was filtered (0.45 um ...
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bioRxiv - Molecular Biology 2022Quote: ... then clarified by centrifugation at 66,000 x g for 30 min using a JA 25.50 rotor (Beckman). The clarified lysate was incubated with 2 mL of pre-equilibrated Protein C affinity resin (Roche ...
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bioRxiv - Biophysics 2023Quote: ... the mixture was centrifugated for 30 min at 12,000 rpm and 4 °C (Beckman J2-21, USA), and iVCL supernatant was collected ...
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bioRxiv - Biochemistry 2023Quote: ... then clarified by centrifugation at 66,000 x g for 30 min using a JA 25.50 rotor (Beckman). The supernatant was incubated with 2 mL of pre-equilibrated Protein C affinity resin (Roche ...
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bioRxiv - Biophysics 2023Quote: ... and centrifuged at 5000 xg for 30 minutes at 4°C and subsequently at 10000 xg (Beckman Coulter Optima L-90K with a SW40Ti rotor) ...
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bioRxiv - Biophysics 2024Quote: ... lysed via sonication and centrifuged at 27,200 g for 30 min using a JA-20 (Beckman Coulter). Soluble protein in the supernatant was purified by Talon metal affinity resin (Takara Bio ...
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bioRxiv - Biochemistry 2024Quote: ... pH 7.4) with specified NaCl concentration and centrifuged for 30 min at 100,000 rpm (TLA100, Beckman Coulter). Unless otherwise stated ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (25000 rpm for 30 min at 4°C using a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (40000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (40000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Bioengineering 2020Quote: ... larger vesicles were depleted by centrifugation for 30 minutes at 10,000xg at 4°C (SW28 rotor, Beckman Coulter). In the final step ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane extract was clarified by spinning at 10,000 g for 30 minutes (Beckman Coulter JA-20 rotor). The supernatant was diluted 3 times with NiNTA A buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was then centrifuged for 30 minutes at 45000 rpm in a Ti45 rotor (Beckman Coulter). Supernatant was then incubated rotating with 1ml of amylose resin (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were cleared by spinning at 3,000xg followed by 20,000xg for 10min each and supernatant were loaded on a 30% sucrose cushion and spun for 1h at 31,200 r.p.m in SW60 rotor (Beckman Coulter). The non-polysomal supernatant was removed and polysomes collected below the sucrose layer was diluted with the hypotonic buffer [10mM HEPES pH 7.8 ...
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bioRxiv - Plant Biology 2022Quote: ... Samples were further pooled into a single tube and 30 µL Ampure XP beads (Beckman Coulter Life Sciences) with 66 µL bead binding buffer (2.5 M NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane extract was clarified by spinning at 10,000 RCF for 30 minutes (Beckman Coulter JA-20 rotor). The supernatant was diluted 3 times with NiNTA A buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Cell Biology 2020Quote: ... Bacteria were spun down at 6000 rpm at 4 °֯C for 30 min in JLA8.1 rotor (Beckman Coulter) in Beckman Avanti J-26S ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane extract was clarified by spinning at 10,000 RCF for 30 minutes (Beckman Coulter JA-20 rotor). The supernatant was diluted 3 times with NiNTA A buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysate was clarified by centrifugation for 30 mins at 16,000 rpm using JLA-16.250 rotor (Beckman Coulter). The supernatant was kept and incubated with equilibrated IgG resin (Smart-Lifesciences ...
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bioRxiv - Microbiology 2024Quote: ... and IAV was then pelleted through a 30% sucrose cushion at 112,400 ×g in a SW31Ti rotor (Beckman) for 90 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Dialyzed nucleosomes were purified in a 5-30% w/v sucrose gradient with a SW41 Ti (Beckman Coulter) rotor in an Optima L-90K ultracentrifuge (Beckman Coulter ...
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bioRxiv - Biophysics 2022Quote: ... Cells were lysed by sonication and the lysate was clarified by centrifugation at 18,000 rpm (25,000 xg) for 30 minutes (JA-20 rotor, Beckman). Supernatant was then applied to a 1 mL HisTrap column (Cytiva) ...
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bioRxiv - Biochemistry 2022Quote: ... Full-length double-stranded cDNA was purified with 30 µl of AMPure XP magnetic beads (Beckman Coulter, #A63881), eluted in 12 µl of nuclease free water and quantify using the dsDNA QuantiFluor Dye System (Promega ...
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bioRxiv - Microbiology 2023Quote: ... and IAV was then pelleted through a 30% sucrose cushion at 112,400 ×g in a SW31Ti rotor (Beckman) or an AH-629 rotor (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane extract was clarified by spinning at 10,000 RCF for 30 minutes (Beckman Coulter JA-20 rotor). The supernatant was diluted 3 times with NiNTA A buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Cell Biology 2023Quote: ... Mitochondrial membranes were pelleted at 72000 xg for 30 min at 4°C using an optimaTM ultracentrifuge (Beckman) with Beckman rotor (TLA 120) ...
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bioRxiv - Biophysics 2024Quote: ... cell pellet was harvested by centrifugation at 4,550 g for 30 min using a JS-4.2 (Beckman Coulter). For protein purification ...
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bioRxiv - Microbiology 2024Quote: ... for 30 min followed by DNA purification using AMPure XP Beads for DNA purification (1X volume, Beckman Coulter). Poly-C tailing was performed followed by DNA cleanup as before ...
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bioRxiv - Microbiology 2024Quote: ... After sonication and centrifugation (30 min at 20,000 rpm; Avanti J-25 centrifuge, Beckman Coulter, Brea, California, USA), cleared lysates were applied to nickel-nitrilotriacetic acid (Ni-NTA ...
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bioRxiv - Neuroscience 2024Quote: ... and 18 ml 1.3 M sucrose / 20 mM Tris/HCl (pH 7.4) and subjected to ultracentrifugation (180,000 x g, for 30 min, SW-32, Beckman). The membrane phase between the two sucrose layers was harvested and diluted with 20 mM Tris/HCl (pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... The insolubilized materials were removed by centrifugation at 164,700 g for 30 min in Ti70 rotor (Beckman Coulter). The solubilized proteins were loaded onto a 2-ml Streptactin beads 4FF (Smart-Lifesciences ...
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bioRxiv - Biophysics 2024Quote: ... cell slurry was sonicated then clarified via centrifugation (66,000g for 30 min) in Type 70 Ti rotor (Beckman). The supernatant was mixed with 2 mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Biophysics 2024Quote: ... and the lysate was cleared by ultracentrifugation for 30 minutes at 4°C (Ti45 rotor, 37,000rpm, Beckman Coulter). The proteins were purified by affinity chromatography using an ÄKTA Start (GE Healthcare ÄKTA Start) ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was then spun at 10,000xg for 30 minutes to pellet the LEVs (Ti45 rotor, Beckman Coulter). This LEV fraction was later washed with PBS spins and used for Western blotting and rescue experiments ...
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bioRxiv - Biophysics 2021Quote: ... the samples were spun in a bench-top Beckman ultracentrifuge at 670,000 g at 20 °C for 30 min (TLA-120.1 rotor, Beckman-Coulter) to pellet F-actin and associated Tpm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (40 000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (25 000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was incubated with GSH beads (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... Fragmented native chromatin was loaded on top of the 9 ml 5% to 40% sucrose gradients and centrifuged for 16 h and 30 min at 37,500 rpm in a SW50.1 ultracentrifuge rotor (Beckman Coulter). Following centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... Bacteria were lysed by sonication and clarified by centrifugation at 20,000 xg for 30 min in a JA-20 rotor (Beckman) at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was clarified by centrifugation at 16,000 rotations per minute (rpm) for 30 min with JLA-16.250 rotor (Beckman Coulter), and the supernatant was incubated with immunoglobulin G (IgG ...
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bioRxiv - Developmental Biology 2020Quote: ... Full-length double-stranded cDNA was purified with 30 μl (0.6x) of AMPure XP magnetic beads (Beckman Coulter, #A63881) and eluted in 20 μl of water.
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bioRxiv - Cell Biology 2020Quote: ... and MTs were pelleted by centrifugation at 126,000 xg for 8 minutes at 30°C in TLA100 (Beckman Coulter). Supernatant was discarded ...
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bioRxiv - Cell Biology 2020Quote: ... Crude EVs were obtained by centrifugation at 20,000 g (30 minutes) or 100,000 g (70 minutes) (Optima XPN Ultracentrifuge, SW 32 Ti Rotor, Beckman Coulter) for large and/or small populations ...
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bioRxiv - Biophysics 2021Quote: ... The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter). After centrifugation the pellet was resuspended in BRB80T.
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bioRxiv - Biophysics 2021Quote: ... The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter). After centrifugation ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed via sonication (Branson Digital Sonifier) and clarified via centrifugation at 66,000 x g for 30 min in a Type 70 Ti rotor (Beckman) at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... the soluble fraction was collected by centrifugation at 40 000 rpm for 30 min in a Ti45 rotor (Beckman). The fusion protein was purified from the soluble fraction using glutathione-Sepharose 4B beads at a vol/vol ratio of 4 ml beads for 100 ml supernatant ...
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bioRxiv - Cell Biology 2021Quote: ... and the mixtures were ultracentrifuged at 80,000 rpm for 5 min at 30 °C using a TLA100 rotor and an Optima TLX ultracentrifuge (Beckman). The microtubule pellets were resuspended in PEM supplemented with 1 mM DTT ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 1.5 mL of anti-Flag M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...