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Citations for Beckman :
1801 - 1842 of 1842 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... were generated using high-speed fluorescence-based cell sorting using various staining and gating strategies as described in the text (MoFlo Astrios, Beckman Coulter, Mississauga, ON).
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bioRxiv - Cell Biology 2021Quote: ... Supernatant was discarded and cells were resuspended in 100 μL FACs buffer and sorted with a CyAn™ ADP Analyser (Beckman Coulter, Inc.) at the UCL GOSICH Flow Cytometry Core Facility ...
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bioRxiv - Biophysics 2022Quote: ... ILC2s were sorted as PI-Lin-CD45+CD127+CRTH2+CD161+ cells via flow cytometry using MoFlo™ XDP (Beckman Coulter, Brea, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with an additional 400 µl 1× Binding Buffer and analyzed within 20 min by CytoFLEX S (Beckman Coulter Life Science). At least 1 × 104 cells were analyzed to determine the percentage of apoptotic cells ...
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bioRxiv - Immunology 2021Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were spun down at 4°C for 15 minutes at 4000 RPM (3040 RCF) in a J6 centrifuge (Beckman Coulter Life Sciences). Supernatant was discarded and pellets were stored in 15ml conical tubes in -80°C ...
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bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 Tl rotor (Beckman ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA was extracted from fresh frozen tissue or cell lines using the Agencourt DNAdvance Beckman Coulter kit (#A48705, Beckman Coulter, Brea, CA, USA), fragmented and submitted to a series of enzymatic steps including End-repair and Adenylation ...
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bioRxiv - Neuroscience 2020Quote: ... 6ml of the cell suspension was carefully pipetted onto the top of the density gradient and centrifuged at 800g (Beckman, Cs-16R Centrifuge) for 30min at 22°C (with acceleration and deceleration rates set at 0) ...
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bioRxiv - Cell Biology 2021Quote: ... The fluorescence generated by the reduction of resazurin to resorufin by live cells was measured using a Beckman coulter DTX 880 multimode plate reader (Beckman Coulter, Brea, California) (570/600 nm).
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bioRxiv - Microbiology 2022Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
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bioRxiv - Microbiology 2022Quote: ... induced by doxycycline (DOX) at 3 μg/ml and sorted for high GFP-expressing cells by MoFLO Astrios EQs (Beckman Coulter Life Sciences) using 488 nm lasers ...
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bioRxiv - Microbiology 2023Quote: ... cells were pelleted by centrifugation at 6,000 ×g at 4 °C for 15 min with ALLEGRA X-30R (Beckman Coulter, Brea, CA, USA). The supernatant was centrifuged at 15,000 ×g at 4 °C for 15 min with Sorvall LYNX4000 (Thermo Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... The disrupted cells were subjected to ultracentrifugation at 4°C and 235000 g for 1h (Beckman Optima™ L-80 XP, rotor Ti45). The resulting supernatant was loaded onto an Äkta purifier system (GE HealthCare ...
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bioRxiv - Biochemistry 2023Quote: ... Expression was continued for 7h and the cells harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000).
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bioRxiv - Biochemistry 2023Quote: ... Expression was continued for 16h and the cells harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000). The RING Zn-finger domains from both RING1B and PCGF4 subunits were cloned into a modified polycistronic expression vector (pET29a ...
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bioRxiv - Cancer Biology 2023Quote: ... Gating strategies were applied accordingly to remove any cell doublets and fluorescent signal was analysed using Kaluza software (Beckman Coulter Life Sciences, U.S.) (Supplementary Figure 1).
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bioRxiv - Evolutionary Biology 2023Quote: ... Five thousand cells from each of the 300 samples were analyzed in 96-well plates in a CytoFLEX Platform flow cytometer (Beckman Coulter, California, USA) at the Feldan Therapeutics facility (Quebec ...
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bioRxiv - Cell Biology 2023Quote: ... The stained cells were resuspended in PBS and analyzed using CytoFLEX S or Galios® flow cytometers (Beckman-Coulter Corp., Hialeah, FL, USA). Data analyses were performed using FlowJo version 10.
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bioRxiv - Microbiology 2024Quote: ... Purified PCR products were measured and pooled for sequencing on an Illumina NextSeq 550 High Output Flow Cell using the single-end 75 base technique using AMPure XP SPRI beads (Beckman Coulter Life Sciences).
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bioRxiv - Neuroscience 2024Quote: Neuron cells obtained from brain organoids were screened for cell surface molecule expression using an ultra-high-speed flow cytometry sorter (Beckman, MoFlo Astrios EQS) as previously reported(Menon et al. ...
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bioRxiv - Microbiology 2024Quote: Cell growth was determined by measuring the absorbance of the culture at A600 (OD600) using a spectrophotometer (DU730, Beckman Coulter, Brea, CA, USA). The concentrations of the products (acetate and ethanol ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated for 30 minutes with the following antibodies: anti-HIV-1 core antigen (p24)-RD1 (Beckman Coulter catalog # 6604667), anti-TNFα PE/Dazzle 594 (BioLegend catalog # 502946) ...
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bioRxiv - Immunology 2023Quote: ... The fluorescence signals for the antibody-receptor complex on the cell surface were then quantified by flow cytometry (CytoFLEX, Beckman Coulter, Brea, CA). Data were analyzed using FlowJo™ v10.8 Software (BD Life Sciences) ...
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bioRxiv - Immunology 2019Quote: ... For patients enrolled in the study, the haemoglobin and red cell indices (MCV, MCH were determined and MCHC) using an automated analyser (Beckman Coulter, Durban, South Africa). CD4-cell counts were assessed using BD FACS Count (BD Biosciences ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We performed a two-step nuclear DNA Content Analysis using Partec Buffer (de Laat et al., 1987) and DAPI fluorochrome through a Cell Lab Quanta SC flow cytometer (Beckman Coulter, Fullerton, CA, USA) equipped with a mercury lamp following the protocol of Doležel et al ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The red blood cell count of birds (number of red blood cells per ml of blood) was quantified immediately before the blood meal using flow cytometry (Beckman Coulter Counter, Series Z1). One hour after the last injection ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was isolated from all TP53-KO minor cells treated in F10 matrigel assays using the Agencourt DNAdvance Genomic DNA Isolation Kit (Beckman Coulter, Cat. No. A48705), and the TP53 locus was amplified using the following primers ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were collected and RNA was isolated using the Biomek FxP and the RNAdvance Tissue Isolation kit (Beckman Coulter, USA, p/n A32646). The resulting RNA was quantified using the Nandrop 8000 (Nanodrop ...
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bioRxiv - Microbiology 2021Quote: ... 50 μL of each cell sample was mixed with 1 μL of KC57-FITC antibody that identifies the HIV-1 Gag and p24 (Beckman Coulter Life Sciences, USA) and incubated at 4°C for 20 min ...
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bioRxiv - Immunology 2021Quote: ... isolated CD4+ cells were activated with a combination of plate-bound anti-CD3 (750 ng/24-well culture plate well; Immunotech/Beckman Coulter REF # IM-1304) and soluble anti-CD28 ((1ug/mL ...
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bioRxiv - Systems Biology 2022Quote: ... supernatant containing metabolites extracted from 1.1×109 cells and 11 spike-ins were aliquoted into 1,000 vials using an automated liquid handler (Biomek 4000, Beckman Coulter, Inc., Brea, California, USA), with 5 μL solution per tube ...
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bioRxiv - Biophysics 2023Quote: ... of 0.6 at 260 nm were loaded into cells fitted with sapphire windows and 12 mm double channel epon charcoal centerpieces (Beckman Coulter, Indianapolis, IN, USA). Data were collected in intensity mode at 260 nm ...
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bioRxiv - Cell Biology 2023Quote: ... where OD550 = 1 corresponds to ∼ 1 × 106 cells ml -1 (David L. Spector, 1998) or a Z1 Coulter Counter (Beckman Coulter Inc., Indianapolis, Indiana). Cultures were generally used during the log phase of cell growth (cell density 2-4 × 105 cells ml-1) ...
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bioRxiv - Immunology 2023Quote: ... Suspended cells were incubated in the dark for 30 min at room temperature with CD66b-APC-Alexa Fluor 750 (Beckman Coulter Life Sciences, B08756) for the detection of neutrophils ...
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bioRxiv - Cell Biology 2023Quote: ... where OD550 = 1 corresponds to ∼ 1 × 106 cells ml -1 (David L. Spector, 1998) or a Z1 Coulter Counter (Beckman Coulter Inc., Indianapolis, Indiana). Cultures were generally used during the log phase of cell growth (cell density 2-4 × 105 cells ml-1).
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bioRxiv - Genomics 2023Quote: ... Cell pellets were processed for genomic DNA extraction following the magnetic bead-based Genfind V3 DNA isolation protocol (Beckman Coulter Life Sciences Cat# C34881).Next-generation sequencing was performed on the Illumina MiSeq platform ...
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bioRxiv - Biophysics 2023Quote: ... Cells were harvested by centrifugation at 8000 g at 4°C for 20 min (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 16.250).
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bioRxiv - Molecular Biology 2023Quote: ... supernatants from the SF samples and cellfree human and equine neutrophil cell culture supernatants were transferred to SW40 tubes (Beckman Coulter Inc., Brea, United States). They were subjected to centrifugation at 10,000 ×g for 35 min at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... for further depletion of cell debris and spun at 10,000 × g for 30 min at 4°C (AH-650 rotor, Beckman Ultra-Clear Tube with 5 ml capacity). The pellet was resuspended in 100 μl PBS and considered to be the “10K pellet” (10K or 10K fraction) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was induced with 0.4 mM Isopropyl β-D-1-thiogalactopyranoside and cells were grown at 37 °C overnight before harvesting by centrifugation at 4000 rpm (Beckman Coulter Avanti JXN-26; JLA8.1 rotor) at 10 °C for 10 min.
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bioRxiv - Biochemistry 2019Quote: ... Protein expression was induced with 0.4 mM Isopropyl β-D-1-thiogalactopyranoside and cells were grown at 25 °C overnight before harvesting by centrifugation at 4000 rpm (Beckman Coulter Avanti JXN-26; JLA8.1 rotor) at 4 °C for 15 min.