Labshake search
Citations for Beckman :
1701 - 1750 of 1842 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Single cells were flow sorted into single wells of 96-well plates using the MoFlo XDP100 cell sorter for ATTO550 positivity (Beckman Coulter) (University of Colorado Anschutz Medical Campus Cancer Center Flow Cytometry Core) ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were subsequently incubated with 2.5×103 CFSE-RJ-EVs per cell for 4 hours and analyzed with flow cytometry (CyAn ADP, Beckman Coulter, US). Treatment groups were compared to unstained control as well as CFSE-RJ-EVs without inhibitor ...
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bioRxiv - Biochemistry 2022Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... cells were seeded into pre-coated wells with anti-TCR pan-γδ clone IMMU510 antibody (5ug/ml) (Beckman Coulter, Krefeld, Germany) and IL-2 (100IU/ml) ...
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bioRxiv - Immunology 2022Quote: ... Cells were pelleted at 400xg for 5 min and washed with PBS twice before flow cytometry (Beckman Coulter CytoFLEX Flow Cytometer). Analysis of phagocytic ability was undertaken using FCS Express.
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bioRxiv - Synthetic Biology 2022Quote: ... in an optimized version of the Light Plate Apparatus (LPA).53 HEK293T cells were analyzed 24h after illumination using CytoFLEX S Flow Cytometer (Beckman Coulter) as described previously.53 For each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... After incubation for 24 hours the cells were harvested and analyzed for fluorescence intensity with a Beckman Coulter Gallios (Beckman Coulter). The result was analyzed by Kaluza Analysis 2.1 (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal optical density units from cleared cell lysates were loaded onto the gradient and centrifuged in a SW40 rotor (Beckman Coulter) for 5 hr ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were harvested after 3 days and counted using a Z2 Coulter Particle Count and Size Analyzer (Beckman Coulter, Brea, CA).
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bioRxiv - Cell Biology 2023Quote: ... Resulting single nuclei of G1 phase (as determined by Hoechst and PI staining) were sorted into single wells of 96 wellplates on a MoFlo Astrios cell sorter (Beckman Coulter) and sorted at −80 degrees until further processing ...
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bioRxiv - Molecular Biology 2022Quote: Nuclei were isolated from 200 cotyledons of 5-day-old seedlings and purified using a two-layer Percoll gradient at 3000 g before staining with 0.5 μM DAPI and sorting by FACS according to their ploidy levels using a MoFlo Astrios EQ Cell Sorter (Beckman Culture) in PuraFlow sheath fluid (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... stained the cells by a Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, A211-01) and detected cells distribution using a Beckman Coulter Gallios (Beckman Coulter). The result was analyzed by Kaluza Analysis 2.1 (Beckman Coulter).
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bioRxiv - Microbiology 2023Quote: ... followed by flow cytometry analysis of CAp24 antigen expression on fixed and permeabilized cells labelled with KC57-fluorescein isothiocyanate (FITC) (Beckman Coulter).
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bioRxiv - Cancer Biology 2022Quote: ... The single muscle cell suspension was sorted by tdTomato+DAPI-(ECs) and tdTomato-DAPI- (non-ECs) using MoFlo Astrios (Beckman Coulter). The sorted cells were loaded into a 10x Genomics microfluidics chip and encapsulated with barcoded oligo-dT– containing gel beads using the 10X Genomics Chromium controller according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Resulting cell nuclei were gated for G1 phase (as determined by Hoechst and PI staining) and single nuclei were sorted into wells of 96-well plates on a MoFlo Astrios cell sorter (Beckman Coulter). 96 well plates containing nuclei and freezing buffer were stored at –80°C until further processing ...
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bioRxiv - Microbiology 2023Quote: ... the cells were resuspended in 300 μL of FACS buffer and analyzed by using the CytoFlex cytometer (Beckman Coulter, Fremont, CA). Approximately 100,000 events were acquired for each sample ...
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bioRxiv - Cell Biology 2023Quote: ... Resulting cell nuclei were gated for G1 phase (as determined by Hoechst and propidium iodide staining) and sorted into wells of 96 wells plates on a MoFlo Astrios cell sorter (Beckman Coulter), depositing one cell per well ...
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bioRxiv - Bioengineering 2023Quote: ... Viable Macrophages (Live+CD45+) and ODCs (Live+CD45-) were then sorted using a Beckman Mo-Flo Astrios cell sorter (Beckman Coulter) directly into 600 µL of RLT lysis buffer with 6 µL of β-mercaptoethanol ...
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bioRxiv - Neuroscience 2023Quote: ... CX3CR1-GFP and GFAP-CFP cells from adult male and female mice were submitted to FACS immediately after dissociation using a MoFlo Astrios EQ (Beckman Coulter) based on their fluorophore (GFP and CFP) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed twice with PBS before resuspension in MACS buffer for subsequent flow cytometric analysis using the CytoFLEX (Beckman Coulter).
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bioRxiv - Microbiology 2023Quote: Flow cytometric counts of the fresh cells were obtained by using a benchtop CytoFLEX S flow cytometer (Beckman Coulter, Brea, USA), equipped with the identical lasers and detectors as described previously [37] ...
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bioRxiv - Cancer Biology 2023Quote: ... The percentages of cells in the different stages of apoptosis were detected by using the CytoFLEX S flow cytometer (Beckman Coulter).
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bioRxiv - Cell Biology 2023Quote: ... This step was followed by centrifugation at 10,000 × g for 20 min to remove cell debris using the Allegra X-30R centrifuge (Beckman Coulter, USA). The supernatant was transferred to ultra-clear thin wall tubes (Beckman Coulter ...
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bioRxiv - Genomics 2023Quote: ... Five million cells were harvested at 315xg for 7 minutes in a 15 mL centrifuge tube (Beckman Coulter Allegra X-14R) and stored at -80°C ...
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bioRxiv - Plant Biology 2023Quote: ... Uptake was terminated by rapid filtering of the cells into the kill solution during 20 s centrifugation at set maximum speed (microfuge B, Beckman, USA). For dark-inactivation experiments ...
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bioRxiv - Biochemistry 2023Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were detached with 0.25 % trypsin and subjected to flow cytometry analysis using CytoFLEX flow cytometer (Beckman Coulter, Brea, CA). Per sample ...
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bioRxiv - Cancer Biology 2023Quote: ... all co-cultured cells were harvested and analyzed for GFP and RFP fluorescence using CytoFLEX flow cytometer (Beckman Coulter, Brea, CA). Per sample ...
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bioRxiv - Biochemistry 2023Quote: ... the cell suspension was further diluted with 400 µl PBS and the stained cells were analyzed using the CytoFLEX flow cytometer (Beckman Coulter). Apoptosis ...
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bioRxiv - Cancer Biology 2023Quote: Counting of CB CD34+ cells after 3 days of ex vivo co-culture was accurately obtained with flow-count fluorosphere beads (Beckman Coulter), in combination with the following antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescence Activated Cell Sorting was performed immediately after dissociation at the Zuckerman Institute Flow Cytometry platform using a MoFlo Astrios EQ (Beckman Coulter) sorter ...
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bioRxiv - Molecular Biology 2023Quote: ... the measure of DNA content and the percentage of GFP positive cells were carried out with Gallios flow cytometer (Beckman Coulter) using the Kaluza Acquisition software (Beckman Coulter) ...
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bioRxiv - Cell Biology 2024Quote: ... The population density of the control and induced TbMyo21 RNAi cells was measured every 24 h over a time course of 96 h using a Z2 Coulter Counter (Beckman Coulter).
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bioRxiv - Biochemistry 2023Quote: GFP fluorescence level (FITC channel, signal amplification = 1) and cell size (FSC channel, signal amplification = 200) were measured with flow cytometer CytoFlex S (Beckman Counter) and CytExpert Software in 10000 cells (per each sample ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted for matched high expression of receptor plus introduced adaptor by fluorescence-activated cell sorting (FACS) (MoFlo Astrios, Beckman Coulter).
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bioRxiv - Cell Biology 2023Quote: ... 20 µL of the cell lysate in each well were transferred to a Proxi plate by a Beckman NX (Beckman Coulter). The plates were then promptly de-ionized (to prevent ESI spray instability ...
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bioRxiv - Synthetic Biology 2023Quote: EGFP transfected cells and HEK-293-loxP-GFP-RFP cells before and after PLP coincubation were measured with a CytoFLEX S cytometer (Beckman Coulter) using 488 nm laser with a 525/50 filter for measuring EGFP expression and a 561 nm laser with a 610/20 filter for measuring RFP ...
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bioRxiv - Microbiology 2024Quote: ... Stained cells were then washed once with PBS and ROS levels were analyzed using a CytoFLEX S flow cytometer (Beckman Coulter) with lasers at wavelengths of 405 nm (violet) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The FITC fluorescence signals demonstrating the antibody-receptor complex on the cell surface were quantified by flow cytometry (CytoFLEX, Beckman Coulter). Relative expression levels of GPR1 mutants were represented according to the fluorescence signals.
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bioRxiv - Cancer Biology 2024Quote: Analysis of GALNT3 or fibronectin expression on human or mouse pancreatic cancer cells was performed using a flow cytometer (Gallios, Beckman Coulter) with appropriate antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... Conventional flow cytometry analysis was performed on 200 µL of whole blood cells using a lyse no wash protocol (Versalyse lysing solution, Beckman Coulter) or on 2 ×106 PBMC labeled and analyzed using a Fortessa cytometer (BD Biosciences ...
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bioRxiv - Genetics 2024Quote: ... Resulting cell nuclei were gated for G1 phase (as determined by Hoechst and propidium iodide staining) and sorted into wells of 96 wells plates containing freezing buffer on a MoFlo Astrios cell sorter (Beckman Coulter). 30 nuclei of each were deposited per well ...
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bioRxiv - Genetics 2024Quote: ... and EGFP fluorescence was measured by FACS on either a SA3800 Spectral Cell Analyzer (Sony Biotechnology) or a CytoFLEX S Flow Cytometer (Beckman Coulter). Typically ...
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bioRxiv - Immunology 2024Quote: Individual CD24hi or CD24low Vγ1.1+ γδ T cells were isolated from Id3+/+ and Id3-/- mice by flow cytometry and deposited in wells of glass slides (Beckman-Coulter). Vγ1.1 and Vδ6.3 subunits were then amplified by RT-PCR using nested primers in single cells ...
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bioRxiv - Immunology 2021Quote: ... the cells were washed and resuspended in RPMI with 10% of SFB and EDTA 0,05mM and submitted to cell sorting in Moflo Astrios® cytometer (Beckman Coulter®). The gate strategy and neutrophil yield are supplied in the supplementary figure 1.
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bioRxiv - Microbiology 2019Quote: ... Cells were permeabilized in 0.5% Triton-X for 10 min and stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987). Data were collected on Accuri C6 (BD Biosciences – U Mass ...
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bioRxiv - Genetics 2019Quote: ... We sorted 30 million cells for each screen into six bins based on fluorescence intensity of target genes using the Astrios EQ Sorter (Beckman Coulter B25982). To control for differences in staining efficiency for each cell ...
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bioRxiv - Genomics 2019Quote: ... Cells were suspended with 300 μl 1% FBS /HBSS (with 10 mM sodium butyrate) and subjected to FACS (Beckman Coulter MoFlo XDP) sorting for tdTomato positive cells ...
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bioRxiv - Cell Biology 2020Quote: ... tSETD2-Flag plasmid was transfected into HEK293 Freestyle cells with FectoPRO transfection reagent (116-010) and cells were harvested 36-48 hours later at 5,000 rpm for 15 mins (Beckman JLA 8.1, 363563). The pellet was suspended in lysis buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... the two initial washes were performed with PBS containing 0.5% Triton x-100 and cells were stained for 30 min with an α-p24 (gag) antibody (Coulter Clone KC57-RD1; Beckman Coulter). Data were acquired with a BD FACSVerse flow cytometer and analyzed using the FlowJo package (FlowJo LLC ...