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Citations for Beckman :
1651 - 1700 of 1842 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Cell suspension was supplemented with 500 μl of 0.1% w/v BSA/PBS for adjustment of the volume prior to the analysis and subjected to flowcytometric analysis using Cell Lab Quanta SC (Beckman Coulter).
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were pelleted down for 10 min at 5000 rpm at 4°C (Rotor: F10-6X500, FiberLite Beckman J2-MC). The pellet was washed with buffer 1 (HEPES- 20 mM ...
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bioRxiv - Cell Biology 2022Quote: ... The cDNA amplification from single cells was performed by PCR for 21 cycles and the amplified products were purified using AMPure XP paramagnetic beads (Beckman Coulter).
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Full hematology with blood cell count (CBC) was performed at each time of bleeding using an HMX A/L (Beckman Coulter). Immunological investigations were performed with fresh whole blood or isolated peripheral blood mononuclear cells (PBMCs).
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bioRxiv - Genetics 2019Quote: The PCR plates containing 25μl of cells per well were thawed and a modified protocol of the Agencourt GenFind V2 (Beckman Coulter, A41499) was utilized to isolate genomic DNA ...
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bioRxiv - Genomics 2020Quote: ... Stained cells were then washed with MACS buffer and the CD45+ Lin-CD38-CD34+ CD45RA-CD90+ fraction (HSCs) was FACS sorted using a MoFlo Astrios Cell Sorter (Beckman Coulter). Single-cell ATAC-seq data was then generated for the sorted HSCs using the dscATAC-seq Whole Cell protocol as previously described14.
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bioRxiv - Evolutionary Biology 2021Quote: ... The equation was established by combining OD measurements (λ = 600 nm) and cell counting in a flow cytometer (Beckman Coulter CytoFLEX) after staining with propidium iodide (PI).
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bioRxiv - Genomics 2021Quote: ... Cells were washed with 25 mm supplemented HBSS before depleting the sample of unnucleated cells using IOTest 3 Lysing solution (Beckman Coulter) following provider instructions ...
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bioRxiv - Neuroscience 2020Quote: ... This incubation step was followed by harvesting cells by centrifugation at 4000 rpm using JLA 8.1000 rotor (Beckman Coulter, Bear, CA) for 30 minutes at 5 °C ...
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bioRxiv - Cell Biology 2019Quote: The concentration of prey and THP-1 cells were measured prior to phagocytosis by flow cytometry (Accuri C6, BD or CytoFLEX, Beckman-Coulter), if needed ...
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bioRxiv - Biochemistry 2020Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 g for 1 hour (Beckman Coulter JA- 20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... by using the single cell sorting option at a pressure of 60 psi on a MoFlo Astrios Flow Cytometer (Beckman Coulter) equipped with a 70 µm jet in air nozzle ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were diluted 100-fold in PBS to reach the final cell concentration of approximately 106 cfu/mL and measured on a CytoFLEX S (Beckman Coulter) using a FITC fluorescence channel with a 488 nm excitation laser and 525/40 nm emission band-pass filter with side scatter triggered events threshold set to 10,000 events ...
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bioRxiv - Systems Biology 2020Quote: ... Single clones of HEK293 cells expressing EPO or GFP were generated from the polyclonal batches by seeding single cells per well of 384-well plates by either limited dilution or FACS (Astrios, Beckman Coulter). In case of GFP-expressing cells ...
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bioRxiv - Microbiology 2020Quote: ... Whole blood staining was done with Alexa Fluor®647 labelled-VLP (2.5µg/ml) and cell subsets were discriminated using the following antibody panel: CD45 (Clone J33; Beckman Coulter, Brea, CA), CD11c (Clone BU15 ...
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bioRxiv - Microbiology 2021Quote: ... the cell suspensions were diluted in 1 mL and loaded onto a cytoflex-LX flow cytometer (Beckman Coulter, Brea, CA, USA) with a 375 nm laser ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell sorting was performed at the University of Colorado Cancer Center Flow Cytometry Shared Resource using an XDP-100 cell sorter (Beckman Coulter).
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bioRxiv - Cancer Biology 2020Quote: ... the cells in the T25 flask were harvested and counted by the Multisizer 4e coulter counter (Beckman Coulter, Brea, CA, USA).
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bioRxiv - Cell Biology 2021Quote: ... or Lin−Sca-1+c-Kit+CD150+CD48−CD34− (CD34−HSC) and Lin−Sca-1+c-Kit+ (LSK) cells were sorted on MoFlo Astrios EQ (Beckman Coulter). Quantification of CD71 ...
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bioRxiv - Molecular Biology 2022Quote: ... 2D10 cells were washed twice with 1 x phosphate buffered saline (PBS) and analyzed by flow cytometer (Epics Altra, BECKMAN COULTER) for the percentages of cells expressing GFP.
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bioRxiv - Microbiology 2022Quote: ... through a syringe needle (0.4-by 20 mm needles) and the cell wall was collected by ultracentrifugation at 90,000 × g for 45 min at 4°C (Beckman Coulter, Inc.). To the pellet ...
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bioRxiv - Biochemistry 2022Quote: ... NanoGlo luciferase assay substrate was prepared at a 1:100 dilution according to manufacturer’s protocol and 3 μl NanoGlo substrate was added to each well of media transfer plate and cell source plate with a BioRAPTR FRD (Beckman Coulter). Nanoluciferase luminescence for each plate was read on a ViewLux plate reader (PerkinElmer ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were incubated for 2h at 20°C in an AN60-Ti rotor in the Optima-AUC analytical ultracentrifuge (Beckman Coulter) before data acquisition for 15h at 42,000 rpm ...
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Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordbioRxiv - Neuroscience 2020Quote: ... were added to each sample well and cells were kept at 4°C until analysis on a Cytoflex Flow Cytometer (Beckman Coulter).
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bioRxiv - Microbiology 2021Quote: ... Growth rates were monitored for 96 h and cell densities were determined every 24 h using a Coulter Counter Z2 particle counter (Beckman Coulter). Procyclic forms (PCF ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were grown for 4-6 days and counted using an Incucyte (Essen Biosciences) or a Z2 Coulter Counter (Beckman Coulter). Small molecule dose-response curves were printed using an D300e Digital Dispenser (HP Specialty Printing Systems ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell sorting was performed at the University of Colorado Cancer Center Flow Cytometry Shared Resource using a MoFlo XDP cell sorter equipped with a 100 micron nozzle (Beckman Coulter). The sorting strategy excluded debris and cell doublets by light scatter and dead cells by DAPI (1 μg/ml) ...
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bioRxiv - Cell Biology 2022Quote: Live Jurkat E6.1 cells were stained with Laurdan dye as detailed above and analyzed by flow cytometry using a Cytoflex S (Beckman Coulter). Cells were resuspended in HBSS without Ca2+ and Mg2+ (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... Several clonal lines were obtained by transduction of the RevVNP cell line with the mentioned particles and flow cytometry sorting (Beckman Coulter). For reproducibility reasons ...
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bioRxiv - Immunology 2019Quote: ... Naïve B cells (CD19+ CD27− IgD+) and unswitched memory B cells (CD19+ CD27+ IgD+) were obtained by FACS sorting on a MoFlo Astrios (Beckman Coulter). Purified samples were pelleted and stored at −80°C.
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The antibiotic bedaquiline activates host macrophage innate immune resistance to bacterial infectionbioRxiv - Immunology 2019Quote: ... Cells were detached and were fixed with 4% paraformaldehyde and the fluorescence was analyzed using a CytoFLEX Flow Cytometer (Beckman Coulter).
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bioRxiv - Cancer Biology 2019Quote: Cells were stained for 30 min (minutes) with the anti-human monoclonal antibody CD11b-PE (IM2581U, Beckman Coulter, Brea, CA, USA), CD14-FITC (555397 ...
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bioRxiv - Immunology 2020Quote: ... the cells were washed twice in cold phosphate buffer (PBS) before suspended in cold PBS and sorted on Moflo Astrios (Beckman Coulter). Sorted cells with different scattering intensity were stained to identify the percentages of CD3+ CD4+ T/CD3+ CD8+ T ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were then filtered through a 40 μm cell strainer and analyzed by flow cytometry (MACSQuant VYB, Miltenyi or CytoFLEX, Beckman Coulter). We used EasyFlow ...
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bioRxiv - Immunology 2021Quote: ... To sort specific subsets of C-LP mononuclear phagocytes cells were gated as above and sorted by MoFlo Astrios Sorter (Beckman Coulter).
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bioRxiv - Immunology 2019Quote: ... Cells were sorted for high expression of receptor ± introduced adaptor or CD8 co-receptor by fluorescence-activated cell sorting (FACS) (MoFlo Astrios, Beckman Coulter).
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bioRxiv - Immunology 2019Quote: ... CHO ligand anchor HLA-A*02 cells were sorted for high expression of ligand anchor and HLA-A*02 using FACS (MoFlo Astrios, Beckman Coulter).
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bioRxiv - Neuroscience 2019Quote: ... Catalog L3224A) and lack of EtD-1 homodimer staining (L3224B) were selected and sorted by a MoFlo Astrios cell sorter (Beckman Coulter) into 500 µL filled into dissecting medium.
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bioRxiv - Biochemistry 2019Quote: ... Haemoglobin and red cell indices (MCV, MCH and MCHC) were determined using an automated haematology analyser (Beckman Coulter, Durban, South Africa). CD4-cell counts were assessed using BD FACS Count (BD Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Immunology 2021Quote: Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter). Test sera were diluted 1:40 in growth medium and serially diluted ...
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bioRxiv - Cell Biology 2021Quote: ... BM analyses were terminal analyses at the time of tissue harvest and BM cellularity was determined using a ViCell automated cell counter (Beckman-Coulter).
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bioRxiv - Immunology 2020Quote: ... B- and NK cells within the lymphocyte population was performed by a whole blood staining lyse-no-wash protocol (Optilyse B, Beckman-Coulter) using six colour flow cytometry with fluorochrome-conjugated antibodies as listed in Supplementary Table 7 top ...
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bioRxiv - Immunology 2021Quote: ... cells were transferred to a 96 wells V-bottom plate and stained in PBS with ViaKrome 808 viability dye (Beckman Coulter). Next ...
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bioRxiv - Immunology 2020Quote: ... during 30min and then transferred into a DuraClone IF T Helper cell tube (CD3, CD4, IL-4, IL-17A, IFNγ) (Beckman Coulter), using the IntraPrep fixation permeabilization kit (Beckman Coulter) ...
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bioRxiv - Immunology 2020Quote: Intracellular cytokine production by T cells was analyzed after stimulating PBMC or whole blood (fresh or frozen) 4h at 37°C in DurActive1 tubes (Beckman Coulter), which contain PMA ...
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bioRxiv - Immunology 2021Quote: ... Insoluble proteins and cell debris were removed by centrifugation (13,000 rpm, 30 minutes at 5°C, JA-30.50Ti rotor, Beckman, Palo Alto, CA). The soluble CuMVTT CP protein in clarified lysate was pelleted using saturated ammonium sulfate (1:1 ...
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bioRxiv - Cell Biology 2021Quote: ... the cell lysate after removal of cell debris at 850 g was first pelleted with an Optima MAX-XP Ultracentrifuge (with TLA-100 rotor, Beckman Coulter) at 35,000 rpm (47,265 g ...
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bioRxiv - Bioengineering 2021Quote: ... cells were resuspended in 100 µL PBSB and data were acquired on a CytoFLEX S flow cytometer (Beckman Coulter, Brea, CA) and analyzed by FlowJo software (FlowJo LLC ...
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bioRxiv - Immunology 2022Quote: ... and total RNA was isolated using Isospin cell and tissue RNA kit (Nippon Gene) or an RNAdvance v2 kit (Beckman Coulter) according to manufacturer instructions and quantified with an RNA HS Assay Kit (Thermo Fisher ...