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Citations for Beckman :
1601 - 1650 of 1842 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Thylakoids were obtained from the supernatant cell homogenate by ultracentrifugation (35 min at 40,000 rpm in a Beckman Ti45 rotor) and thylakoid-containing pellets were suspended in Chelex-100-treated breaking buffer to a concentration of 1.0-1.5 mg of Chl mL-1 with [Chl] determined from methanol extracts according to (54) ...
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bioRxiv - Immunology 2020Quote: ... Absolute cell number was enumerated using a bead-based counting assay with Flow-Count™ fluorospheres (Beckman Coulter, CA, USA) as described [14] ...
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bioRxiv - Immunology 2022Quote: ... Every 4 days cells were fed by replacing 25 μl of media with fresh I10 containing irradiated 2.5 × 103 3T3-msCD40L cells and 50 ng/mL IL-21 using a Biomek NXP liquid handler (Beckman Coulter). Cells were cultured for 2-3 weeks before harvesting the final supernatant and either freezing the cultures in the 384-well plate (50ul freezing media per well (FBS + 10% DMSO)) ...
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bioRxiv - Biochemistry 2019Quote: Algal cell density and optical density at 730 nm were measured using a Z2 particle count analyser (Beckman Coulter Ltd.) with limits of 2.974-9.001 µm ...
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bioRxiv - Cancer Biology 2020Quote: ... cellular nucleic acids from each lysed single cell were extracted from TCL lysis buffer using a 2.2x (v/v) RNA SPRI (RNA-clean AMPure beads, Beckman-Coulter). After ...
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bioRxiv - Plant Biology 2020Quote: ... The cells were disrupted in a French Press at 1500 psi and centrifuged at 45,000 rpm (Beckman 70 Ti rotor). The supernatant was loaded onto an amylose column (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... The treated cells were pelleted by centrifugation at 12,000×g at 4 °C for 20 min (Beckman Allegra X-15R) and the supernatant was discarded ...
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bioRxiv - Biophysics 2021Quote: ... Cells were lysed and the lysate was clarified by centrifugation for 1 h at 100,000g (45 Ti rotor, Beckman Coulter). The clarified lysate was incubated with Ni2+-NTA agarose resin for 1 h at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... The insoluble cell debris was removed via centrifugation for 30 min at 4°C and 20,000 rpm (JA-25.50, Beckman Coulter®). The supernatant was incubated for 1 h with low agitation and with 1.2 ml Glutathione-sepharose (GE-Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... the cells were harvested by centrifuging for 30 min at 4°C and 6,000 rpm (JA-10, Beckman Coulter®). The cells were resuspended in 1x PBS (pH 7.3 ...
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bioRxiv - Microbiology 2019Quote: The density of the culture (A600) after cell growth was tested using a spectrophotometer (DU730, Beckman Coulter, Placentia, California, USA). The concentrations of the solvents (acetone ...
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bioRxiv - Cancer Biology 2019Quote: ... All 384 single-cell libraries in each batch were pooled and cleaned up with 0.6:1 Ampure XP beads (Beckman Coulter). The multiplexed RNA-seq library was normalized to 4nM with Suspension Buffer (provided in Nextera XT kit ...
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bioRxiv - Cancer Biology 2020Quote: ... The virally-transduced human CD34+ were sorted for GFP reporter expresssion on a MoFlo high-speed cell sorter (Beckman-Coulter) located at the New York Blood Center ...
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bioRxiv - Microbiology 2021Quote: ... bacterial cells were washed in PBS and diluted 1:5 before acquisition on a CytoFLEX benchtop flow cytometer (Beckman Coulter). For each sample ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then harvested by centrifugation at 8000 rpm (Avanti® JE centrifuge and JLA-16.250 rotor from Beckman Coulter) for 10 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The cell culture media containing viruses were filtered (0.45 μm) and purified by 25% sucrose using an SW28 rotor (Beckman Coulter) at 141,000g for 90 min ...
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bioRxiv - Microbiology 2020Quote: ... Whole-cell lysates were then spun at 35,000 rpm for 45 min in a Type 45 Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with following antibody mix for 30 minutes on ice: pan-γδTCR-PE (1:10; IMMU510; Beckman Coultier) and CD69-APC (1:25 ...
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bioRxiv - Genetics 2020Quote: ... After two washes with FACS buffer cells were re-suspended on FACS buffer and assessed on CytoFLEX flow cytometer (Beckman Coulter ...
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bioRxiv - Bioengineering 2022Quote: ... MRX-2843 and methotrexate were then transferred to these cell + vincristine microplates using a Beckman NX Liquid Handler (Beckman Coulter) at the Emory Chemical Biology Discovery Center ...
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bioRxiv - Biophysics 2022Quote: ... The cell suspension was boiled for 20 min and centrifuged at 13500 rpm with a JA-20 rotor (Beckman Coulter). Streptomycin sulfate was added to the supernatant to a final concentration of 10 mg/mL and the mixture was stirred for 15 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were centrifuged at 1500 g for 5 min in an F2402 rotor in a GS15R table-top centrifuge (Beckman) and resuspend with water to wash the cells ...
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bioRxiv - Immunology 2022Quote: ... the cells were washed twice in PBS and then labelled with anti-CD3 ECD (clone UCHT1; Beckman Coulter, Brea, CA), anti-CD4 APC Alx750 (clone 13B8.2 ...
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bioRxiv - Immunology 2022Quote: ... αCD21 biotinylated (eBiosciene) and Streptavidin Cy5 (ImmunoResearch) and isolated with a purity >99% with the MoFlo cell sorter (Beckman Coulter). Splenic naïve B cells were isolated by magnetic cell sorting using the “EasySep™ Mouse B Cell Isolation Kit” from STEMCELL according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: Cell growth was measured based on the absorbance of the culture at A600 (OD600) using a spectrophotometer (DU730; Beckman Coulter). The fermentation products were assayed as previously reported (19) ...
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bioRxiv - Immunology 2022Quote: ... TCRVβ14+ CD4+ naïve T cells from spleen and lymph nodes of TCR Tg mice using the MoFlo Astrios or MoFlo Astrios EQ (Beckman) at the Flow Cytometry Core in the UIC Research Resources Center (RRC) ...
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bioRxiv - Molecular Biology 2022Quote: ... or a 384-well plate containing 1x Takara lysis buffer (STORM-seq) using a MoFlo Astrios cell sorter running Summit v6.3 (Beckman Coulter). The Astrios was equipped with a 100 um nozzle at 25 psi ...
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bioRxiv - Biochemistry 2023Quote: ... The amount of radioactivity in the cell lysate was measured using a liquid scintillation counter (Beckman Coulter, Brea, CA, USA) and protein concentration was determined via the Lowry method54 ...
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bioRxiv - Cancer Biology 2023Quote: ... live cells that were either GFP+ or RFP+ using a CytoFLEX S flow cytometer with CytExpert v2.4 software (Beckman Coulter). Analysis was performed using FlowJo v10.7 software (BD Life Sciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... 7,500 GFP+ and 7,500 RFP+ single live cells were sorted into each well of a 24 well culture plate containing low pH DMEM with 10% FBS using a MoFlo Astrios cell sorter with Summit v6.3 software (Beckman Coulter). Sorting was performed at 25psi using a 100um nozzle ...
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bioRxiv - Cell Biology 2023Quote: ... whole cells (per sample) were pelleted via centrifugation at 1,700 xg (JA 20 rotor, Beckman Coulter Inc, Brea, CA, USA) for 10 min at room temperature ...
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Mechanism of RanGTP priming H2A-H2B release from Kap114 in an atypical RanGTP•Kap114•H2A-H2B complexbioRxiv - Biochemistry 2023Quote: ... Cells were lysed with an Emulsiflex-C5 cell homogenizer (Avestin) and centrifuged in an Avanti J-26 XPI with a JA-25.50 rotor (Beckman Coulter) at 20,000 RCF for 1 h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Ramos B cells were double-stained with Alexa 488-cojugated Nef and anti-CD19-PC-5.5 (J3-119, Beckman Coulter), and interacting infected cell-invaded B cell doublet images acquired with ImageStream X MKII (Amnis/Merck Millipore ...
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bioRxiv - Cancer Biology 2023Quote: Cell death was evaluated 48 hrs after ionizing radiation (10 Gy) by using a CytoFLEX S (Beckman Coulter, Milan, Italy) instrument ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded into 6-well plates at a concentration of 4×105 cells per well and counted cumulatively with a Coulter counter (Beckman) every three days for two weeks ...
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bioRxiv - Microbiology 2023Quote: ... 10,000 ACE2-expressinglive cells (gated based on Flag-fluorescence intensity and SSC/FSC) were analyzed using a CytoFLEX Flow Cytometer (Beckman).
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bioRxiv - Immunology 2023Quote: Flow cytometry analysis was performed at the end of the 7 day T cell progenitor differentiation stage using a CytoFLEX LX cytometer (Beckman), and data were compensated using single-stained controls in CytExpert v2.3 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Washed cells were then resuspended in PBS/BSA for analysis by flow cytometry on a CytoFlex flow cytometer (Beckman Coulter). Intact cells were identified based on their forward scatter/side scatter profile and staining intensity was monitored in the APC channel (FL4) ...
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bioRxiv - Immunology 2023Quote: ... Then this clear cell lysate was loaded on top the gradient and ultracentrifugation was done for 5hrs at 133,000g on SW60Ti (Beckman Coulter) rotor to separate each gradient ...
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bioRxiv - Cancer Biology 2023Quote: ... migrated cells to the lower chambers were counted as reported elsewhere [58] with fluorescent microbeads (Flow-Count Fluorospheres, Beckman Coulter) by flow cytometry.
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bioRxiv - Bioengineering 2023Quote: ... The cell uptake of nanoparticles were analyzed by flow cytometry using a CytoFLEX™ flow cytometer (Beckman Coulter, California, USA). For confocal imaging ...
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bioRxiv - Cancer Biology 2023Quote: ... the numbers of GFP+ and DsRed+ cells were determined by flow cytometry on a CytoFlex S Flow Cytometer (Beckman Coulter). For gating strategy see Supplementary Figure 4 in Supplementary Information ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were harvested at 6000 rpm for 30 min in a JLA 9.1000 rotor (Beckman Coulter, Brea, California, USA), washed with 150 mM NaCl ...
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bioRxiv - Pathology 2024Quote: ... Ter 119 and GPIb-beta expression on the membrane of circulating cells were performed by flow cytometry (Gallios, Beckman Coulter).
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bioRxiv - Cancer Biology 2024Quote: ... cells were resuspended in PBS with 0.02% EDTA for cell cycle analysis using a Beckman Coulter Galios 561 analyzer (Beckman Coulter).
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bioRxiv - Biochemistry 2024Quote: ... The indicated cell lines were seeded into 6 well plates and then trypsinized and cell number counted using a Z Coulter Counter (Beckman). Approximately 200 cells were seeded into 60 mm dishes and exposed 25 ...
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bioRxiv - Microbiology 2024Quote: ... The flow cytometry analysis of the bacterial cells present in the suspension was performed using a Cytoflex flow cytometer (CytoFLEX 3; Beckman) as previously described (Supplementary Fig ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated with fluorophore-conjugated antibodies for 30min (see Supplementary Table 1) and FACS-sorted using MoFlo Legacy or Astrios cell sorters (Beckman Coulter). Cells were sequentially gated based on size and granularity (forward scatter ...
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bioRxiv - Cell Biology 2022Quote: ... Yeast cells were stained with 5ug\ml propidium iodide for 10 minutes and samples were examined in CytoFlex flow cytometer (Beckman Coulter) using PE channel ...
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bioRxiv - Neuroscience 2021Quote: The cell suspension was passed through a 50 μm celltrix filter (Sysmex-Partec # 04-0042-2317) immediately before FACsorting the GFP+ NSCs using a MoFlo XDP cell sorter (Beckman Coulter) in the NYU Cytometry core facility ...