Labshake search
Citations for Beckman :
1551 - 1600 of 1842 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... Cells were then analyzed using flow cytometry (CytoFLEX S flow cytometer using CytExpert Software, Beckman Coulter, Brea, CA, USA).
-
bioRxiv - Microbiology 2022Quote: ... the stained cells were detached by 5 mM of EDTA/PBS and analyzed with a CytoFLEX Flow Cytometer (Beckman). 10,000 events in a gated live cell population (based on SSC/FSC ...
-
bioRxiv - Genomics 2023Quote: ... the percentage of cells expressing ZsGreen was quantified three days post-transduction by flow cytometry using Cytoflex S (Beckman) flow cytometer ...
-
bioRxiv - Genomics 2023Quote: ... and selection was verified by ZsGreen expression in >95% of the cells by flow cytometry (Cytoflex S, Beckman Coulter).
-
bioRxiv - Genomics 2023Quote: ... and selection was verified by ZsGreen expression in >95% of the cells by flow cytometry (Cytoflex S, Beckman Coulter). Cells were cultured in growth media for an additional 6 days before processing for scRNA-seq.
-
bioRxiv - Physiology 2023Quote: ... Voltage stimulation and whole-cell recording were achieved using the PatchControl384 v1.5.3 software (Nanion Technologies) and the Biomek v1.0 interface (Beckman Coulter, France). The intracellular solution contained (in mmol/L) ...
-
bioRxiv - Cell Biology 2024Quote: ... A volume of 0.4 μL of ligand was added to cell plates using a BiomekFX Lab Automation Workstation (Beckman) and a previously calibrated pin tool ...
-
bioRxiv - Cancer Biology 2023Quote: ... Percentage of cells with each DNA content (2N, 3N, 4N, 5-8N) were estimated using CytExpert software (Beckman Coulter). For DNA content analysis using sorted cells ...
-
bioRxiv - Systems Biology 2023Quote: The growth of cultures was monitored as increase in cell number using a Coulter Counter model Z2 (Beckman Coulter). Cell size analysis was performed using a Coulter Z2 Particle Cell Analyzer (Beckman-Coulter) ...
-
bioRxiv - Immunology 2023Quote: ... the EHT time point pool was subject to Fluorescent Activated Cell Sorting (FACS) using a MoFlo Astrios (Beckman Coulter). Cell pools were counted and loaded on the Chromium Controller (10x genomics ...
-
bioRxiv - Biochemistry 2023Quote: ... at 18000 PSI and cell debris were separated by centrifugation (4 °C, 30 min, 75,600 ×g, JA25.50 rotor Beckman Coulter). The supernatant was loaded on an affinity chromatography column (GE Healthcare ...
-
bioRxiv - Immunology 2024Quote: In functional assays proportion of infected CD4+ T cells was address by intracellular staining of p24 (KC57, Beckman Coulter) in combination with anti-CD3 ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cells were washed and resuspended in FACS buffer before being analyzed through flow cytometry (Beckman Coulter, Pasadena, USA). For immunofluorescence staining ...
-
2P-NucTag: on-demand phototagging for molecular analysis of functionally identified cortical neuronsbioRxiv - Neuroscience 2024Quote: The sorting was performed at the Zuckerman Institute Flow Cytometry Core using a MoFlo Astrios Cell Sorter (Beckman Coulter). Event rates were kept between 5000-10000 events per second ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cell abundance and equivalent spherical diameter (ESD) were measured on a Coulter® Counter (Beckman Coulter, Fullerton, CA USA) using the ‘narrow’ size range (4–30 μm) ...
-
bioRxiv - Cancer Biology 2024Quote: The distribution of cell cycle phases was observed using a CytoFlex B2-R2-V0 flow cytometer (Beckman Coulter, USA) after fixation in 80% ice-cold ethanol ...
-
bioRxiv - Immunology 2024Quote: Cells were washed in DPBS plus 0.5% human serum albumin and incubated with anti-CD45-KrO (#B36294, Beckman Coulter), anti-CD3-APC (#IM2467 ...
-
bioRxiv - Microbiology 2024Quote: ... Immunostained cells were subjected to a flow cytometric analysis using FACScan flow cytometer (CytoFLEX, Beckman Coulter, Brea, CA, USA). For each assay ...
-
bioRxiv - Cancer Biology 2021Quote: ... The resulting cell extracts were layered over a 20% sucrose cushion (polysome buffer + 20% w/v sucrose) and ultracentrifuged (Beckman Coulter TLA100.3 Fixed-Angle Rotor ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were analyzed by flow cytometry weekly prior to functional assays by using anti-pan γδ TCR-PE (Beckman Coulter), anti-αβTCR-FITC (eBioscience) ...
-
bioRxiv - Cell Biology 2020Quote: ... they were mixed (total 160 μl), applied into a 10-mm path-length cell (105.201-QS, Hellma Analytics) in a DU720 spectrophotometer (Beckman Coulter), and then immediately measured with monitoring the optical density changes at 400 nm (ΔOD400 ...
-
bioRxiv - Biochemistry 2022Quote: ... 2 s off at 30 % amplitude for 10 min and cell debris was removed by centrifugation at 130,000 ×g for 45 min with a Type 70 Ti Rotor (Beckman Coulter). The supernatant was filtered through a 0.22 μm filter (Sigma ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were resuspended in BD perm/wash buffer for subjecting to flowcytometric analysis using Cell Lab Quanta SC (Beckman Coulter). Cell cycle was determined by DNA contents ...
-
bioRxiv - Biophysics 2022Quote: ... The cell suspension was centrifuged for 5 min at 2000 g (Centrifuge Beckman Coulter Allegra X-15-R, Rotor Sx4750A)) and 4°C ...
-
bioRxiv - Genomics 2019Quote: ... wells containing unique cell-specific adapters are pooled using a multichannel pipette and incubated with 0.8x Agencourt Ampure (Beckman Coulter) beads for 30 minutes ...
-
bioRxiv - Cell Biology 2020Quote: ... 5 × 105 additional cells were immediately processed for far red signal analysis by flow cytometry (Gallios, Beckman Coulter, Brea, USA) (D0) ...
-
bioRxiv - Microbiology 2019Quote: ... The bacterial cells were recovered by centrifugation (in a JLA 81000 rotor, Beckman-Coulter, at 6 Krpm for 15 min) and disrupted by sonication (VibraCell 75042 sonicator ...
-
bioRxiv - Genetics 2019Quote: ... We isolated RNA from 100,000 cells in RLT with 2.2X volume Agencourt RNAClean XP SPRI beads (Beckman Coulter, Danvers, MA). As measured by the RNA Qubit High Sensitivity Kit ...
-
bioRxiv - Cancer Biology 2019Quote: ... the cells were trypsinized and counted at specified time points by Z2 Coulter particle counter (Beckman Coulter, Brea, CA, USA). LacZ cells were served as controls ...
-
bioRxiv - Physiology 2020Quote: ... DNA fluorescence was measured using a CytoFlex flow cytometer and the cell cycle analysis was performed by CytExpert v1.2 software (Beckman Coulter).
-
bioRxiv - Physiology 2020Quote: ... BrdU fluorescence was measured using a CytoFlex flow cytometer and the cell cycle analysis was performed by CytExpert v1.2 software (Beckman Coulter). All samples were counterstained with propidium iodide (PI ...
-
bioRxiv - Immunology 2019Quote: ... NK cells were then stained with mAbs to identify subsets based on the expression of KIR2DL1/S1 (Beckman Coulter, EB6), Receptor (R ...
-
bioRxiv - Cancer Biology 2020Quote: The barcode split of the cDNA amplification reaction (from 10x Single Cell 3’ v3 Step 2.2) was purified via 1.2x SPRI Select (Beckman Coulter #B23317). cDNA products were eluted in 40 µL of EB ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were analyzed at the University of Colorado Cancer Center Flow Cytometry Core using a Gallios flow cytometer (Beckman Coulter). All flow cytometry experiments included single stain cell controls ...
-
bioRxiv - Biochemistry 2021Quote: ... The cells were trypsinized and the fluorescence of PAD6 (Ex/Em: 405/525 nm) was analyzed by flow cytometry (CytoFlex, Beckman).
-
bioRxiv - Biophysics 2021Quote: ... The cell suspension was boiled for 20 min and centrifuged at 13,500 rpm with a JA-20 rotor (Beckman Coulter). Streptomycin sulfate was added to the supernatant to a final concentration of 10 mg/mL and the mixture was stirred for 15 min at 4 °C ...
-
bioRxiv - Biophysics 2021Quote: ... The cell suspension was boiled for 20 min and centrifuged at 13,500 rpm with a JA-20 rotor (Beckman Coulter). Streptomycin sulfate was added to the supernatant to a final concentration of 10 mg/mL and the mixture was stirred for 15 min at 4 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... The cells were washed thrice with PBS and the fluorescence profiles were acquired in the Gallios FACS analyzer (Beckman Coulter). All the FACS data were analyzed using FlowJo software (BD Biosciences).
-
bioRxiv - Cell Biology 2021Quote: ... coli cells obtained from 500 mL culture were harvested using centrifugation for 10 min at 4800 rpm (Beckman, Allegra-30XR), and were subjected to freezing in liquid nitrogen ...
-
bioRxiv - Systems Biology 2020Quote: ... GFP productivity was determined by measuring the GFP signal (FL-1 channel) of cells by flow cytometry (Gallios, Beckman Coulter).
-
bioRxiv - Microbiology 2020Quote: ... lysate was collected and centrifuged at 3000x rpm for 20 minutes to pellet cell debris (Beckman Coulter Allegra X-14R). This procedure was repeated for a second passage working stock with collection at 48hpi and titered by TCID50 assay.
-
bioRxiv - Microbiology 2020Quote: ... At the end of each selection cells were pelleted from the media by centrifugation 200 x g for 5 min in an Allegra centrifuge (Beckman) and supernatant was transferred for storage at −80C ...
-
bioRxiv - Microbiology 2021Quote: ... cell lysate was centrifuged 20 min at 14,000 rpm and 4°C to sediment cell debris and supernatant was transferred to microcentrifuge polypropylene tubes (Beckman Coulter) and cytoplasmic membranes were precipitated by ultracentrifugation for 45 min at 55,000 rpm and 4°C (Beckman Coulter rotor TLA 55) ...
-
bioRxiv - Cell Biology 2020Quote: ... cells and larger debris were removed by 2000 × g centrifugation of cell suspensions for 15 minutes (Rotor: JS-5.3; Beckman Coulter). MSC-free CM were stored at −20°C until usage ...
-
bioRxiv - Genomics 2022Quote: ... PCR products with no hashtag libraries (spleen, mouse lung, and human lung cells) were combined and purified twice with 0.65× AmPure XP beads (Beckman Coulter) and eluted with 21 μL of nuclease-free water ...
-
bioRxiv - Molecular Biology 2022Quote: ... Stained cells and fluorescence minus one controls were then resuspended in SB + 1:1000 DNase + 1:1000 Draq7 (Beckman Coulter) as a live/dead stain ...
-
bioRxiv - Microbiology 2022Quote: Algal cell density and optical density at 730 nm were measured using a Z2 particle count analyser (Beckman Coulter Ltd.) with limits of 2.974 - 9.001 μm ...
-
bioRxiv - Bioengineering 2022Quote: ... The frequency of proliferating T cells was determined after 96 hours by flow cytometry using a CytoFLEX II (Beckman Coulter). Zombie Green (Biolegend ...
-
bioRxiv - Biochemistry 2022Quote: ... The lysed cells were centrifuged at 41399 g and 6 °C and for 1 hour (Beckman Avanti J-25 centrifuge) to obtain the cytoplasmic protein extract.
-
bioRxiv - Microbiology 2020Quote: ... Cell containing supernatant was recovered into additional falcon tubes and centrifuged at 10,000G for 20 minutes with 4ºC cooling to pellet cells using an Avanti JXN-30 highspeed centrifuge with JA 14.50 fixed angle rotor (Beckman Coulter). Supernatant was removed without disturbing the cell pellet ...