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Citations for Beckman :
1551 - 1600 of 1603 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The remaining cells were pelleted using a 4300 × g spin for 10 min in an Allegra X-14R centrifuge (Beckman Coulter) equipped with a swinging bucket SX4750 rotor and then resuspended in an equivalent volume of complete BSK-II medium adjusted to pH 6.0 as described above ...
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bioRxiv - Plant Biology 2024Quote: ... The index PCR was stopped after 8–10 cycles and the library DNA was purified using AMPure XP (A63880, Beckman Coulter). The library was sequenced on an Illumina NovaSeq 6000 instrument (150-bp paired-ends ...
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Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... were prepared and cooled on ice. The 10-50% sucrose gradients (vol. = 12 mL) were formed in SW40 ultracentrifuge tubes (Beckman Coulter) using Gradient Station (Biocomp) ...
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bioRxiv - Immunology 2024Quote: ... lysis of gametes and C1q deposition on gametes were assessed by flow cytometry by analysing a minimum of 2,000 gametes with the Gallios™ 10-color system (Beckman Coulter) and analyzed with FlowJo (BD ...
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bioRxiv - Biochemistry 2024Quote: ... Digested pellets were loaded onto a 10-50% sucrose gradient (sucrose dissolved in 1× lysis buffer) and ultracentrifuged using a SW41 Ti rotor (Beckman Coulter) at 40,000 rpm (273,620 × g ...
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bioRxiv - Cell Biology 2024Quote: ... cells on either s2d1 or s6d15 were single-cell dispersed by treating them with TrypLE for 10 minutes at 37°C and assessed to ensure at least 90% viability (Vi-Cell XR, Beckman Coulter). Buffers and regents were prepared fresh according to the 10X Genomics sample prep protocol (CG000505) ...
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Inhibition of Ribosome Biogenesis in vivo Causes p53-Dependent Death and p53-Independent DysfunctionbioRxiv - Cell Biology 2024Quote: ... 1 mL of the lysates was loaded onto a 10%-50% sucrose gradient in a 14 mL polypropylene tube (Beckman Coulter). Ultracentrifugation was performed using a Beckman Optima LE-80K Ultracentrifuge Floor Centrifuge at 261,000 × g for 135 minutes at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... the cells were washed and resuspended in RPMI with 10% of SFB and EDTA 0,05mM and submitted to cell sorting in Moflo Astrios® cytometer (Beckman Coulter®). The gate strategy and neutrophil yield are supplied in the supplementary figure 1.
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bioRxiv - Genetics 2021Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase with addition of the buffer incubated for 60 min at 37 °C followed by inactivation with 1 μL of 10% SDS and purification using RNA clean SPRI beads (Beckman Coulter, A63987).
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bioRxiv - Molecular Biology 2020Quote: ... The resulting cDNA was PCR-amplified to about 10–20 ng/μl using a high-fidelity DNA polymerase and purified with the Agencourt AMPure XP Kit (Beckman Coulter Genomics). The cDNA samples were pooled based on the ratios according to the RNA concentrations of the input samples ...
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bioRxiv - Biochemistry 2020Quote: ... was layered on NaBr δ=1.28 g/mL and centrifuged at 207,000g for 22 h at 10 °C on a Beckman L8M centrifuge (Beckman, Palo Alto, CA). A tube layered with 20 mM Tris-HCl in lieu of the sample was used as a blank for density calculations ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor) to separate the soluble and detergent-enriched phases ...
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bioRxiv - Molecular Biology 2020Quote: ... diluted in 9 mL of 10 mM Tris-HCl buffer and centrifuged for 45 min at 45.000 rpm (Beckman 70.1 Ti Rotor). Pellets were suspended in the same buffer and analyzed by SDS-PAGE using 12.5% polyacrylamide gel ...
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bioRxiv - Microbiology 2021Quote: ... RNA in the aqueous phase was purified and collected in 10 μL of RNase-free water using Agencourt RNAClean XP (Beckman Coulter, A63987) according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2020Quote: ... Samples were eluted in 20 μL of elution buffer and 10 μL of each sample was pooled and concentrated to 20 μL using 0.7x AMPureXP beads (Beckman Coulter, Brea, CA). The final pooled sample was quantified using a Qubit Fluorometer and High Sensitivity DNA assay (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... supernatant containing pseudovirions was harvested by centrifugation at 2,000 rpm for 10 min and concentrated by Optima XPN-100 Ultracentrifuge (Beckman Coulter, California, USA). For pseudovirus infection ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor) to separate the soluble and detergent-enriched phases ...
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bioRxiv - Microbiology 2022Quote: ... Cells were permeabilized in 0.5% Triton-X for 10 min and stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987) or 1:300 KC57-RD1 (numbers) ...
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bioRxiv - Microbiology 2024Quote: ... The resulting cDNAs were PCR-amplified to about 10-20ng/µl using a high-fidelity DNA polymerase and purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics). Pooled libraries were sequenced on an Illumina NextSeq 500 platform at the Core Unit SysMed at the University of Würzburg for bacterial in vitro samples and on an Illumina Novaseq at the genome analytics core facility of the Helmholtz Centre for Infection Research (HZI ...
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bioRxiv - Neuroscience 2023Quote: ... The nuclei suspension was layered onto 10 mL of 29% OptiPrep solution (Buffer + 50% OptiPrep) in a 38.5 mL Ultra-Clear tube (Beckman-Coulter, CA, USA) before being centrifuged at 7,333g for 30 minutes at 4°C ...
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HIV-2 Immature Particle Morphology Provides Insights into Gag Lattice Stability and Virus MaturationbioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in STE buffer and banded out over an OptiPrepTM step gradient (10, 20, 30% OptiPrepTM (v/v)) with a SW55 Ti rotor (Beckman Coulter, CA) at 45,000 rpm for 3 h ...
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bioRxiv - Cell Biology 2023Quote: ... 150 μL of the recovered sample was mixed with 150 μL of 60% Optiprep and layered with 200 μL of 10% Optiprep and 150 μL of the reconstitution buffer in a 0.8 mL tube (Beckman coulter Cat. #344090). The tube was centrifuged at 40,000rpm for 90 minutes in a SW-55 rotor ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were permeabilized for intracellular staining via resuspension in 0.5% Triton X-100 in PBS for 10 min and were subsequently resuspended in a 1:500 dilution of anti-p24-FITC KC57 antibody (Beckman Coulter #6604665) in 1% BSA in PBS for 1 h at room temperature in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated at RT for 30 minutes and centrifugated at 20,000 x g for 10 minutes (Avanti J-30I Centrifuge, Beckman Coulter, Brea, CA). The supernatant was collected and diluted 1:5 with incubation buffer (final concentration ∼204 cells mL-1) ...
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bioRxiv - Microbiology 2024Quote: ... bacterial culture volumes ranging from 10 to 100 µl were diluted in 12 ml of Isotone II diluent in a Coulter counter cuvette (Beckman Coulter, 580015). The diluted samples were then vortexed at a medium speed ...
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bioRxiv - Plant Biology 2024Quote: ... The cell lysate was centrifuged at about 16000 g for 10 min at 5 °C (Avanti J-265 XP, Beckman Coulter, USA). The starch pellet was recovered ...
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bioRxiv - Cell Biology 2024Quote: ... conditioned media were subjected to 10 min at 200g for 3 cycles (G146D centrifuge, Gelec®) and 20 min at 2000g (Allegra 64R Centrifuge, Beckman Coulter) to remove cellular debris and apoptotic bodies respectively ...
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bioRxiv - Synthetic Biology 2024Quote: ... The pellets were resuspended in TEMB buffer and then loaded onto sucrose gradients (10−50%, w/v) followed by ultracentrifugation (Beckman, XL100K ultracentrifuge) at 105,000 g for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Cells were collected at OD600 1.2 by centrifugation at 8000 rpm for 10 minutes (Beckman Coulter Avanti J-15R, rotor JA-10.100). Cell pellets were resuspended in 200 µl gradient buffer containing 20 mM Tris (pH 7.4 at 4°C) ...
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bioRxiv - Cancer Biology 2024Quote: Each selected compound (300 nL) was dissolved in 100% DMSO from an initial stock (10 mM) using Echo® Acoustic Liquid Handling (Beckman Coulter). Next ...
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bioRxiv - Cell Biology 2024Quote: ... Debris in the filtered homogenate was pelleted at 1,000 x g for 10 min using an Allegra X-30R centrifuge (Beckman Coulter Life Sciences). Microsomes were enriched at 200 k x g for 20 minutes at 4°C using a Beckman Optima Ultracentrifuge with TLA110 rotor (Beckman Coulter Life Sciences ...
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bioRxiv - Genetics 2024Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase with addition of the buffer incubated for 60 min at 37 °C followed by inactivation with 1 μL of 10% SDS and purification using RNA clean Ampure XP beads (Beckman Coulter, A63987).
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bioRxiv - Immunology 2021Quote: Immune cell types in Cohort 1 were measured from whole blood by 10-color flow cytometry with Navios flow cytometer (Beckman Coulter, CA, USA). Staining and gating strategies were previously described in detail by Aguirre-Gamboa et al ...
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bioRxiv - Bioengineering 2021Quote: ... 10 µl of which was added to 10 ml of ISOTON® diluent and counted on the COULTER COUNTER® Z1 Series Particle Counter (Beckman Coulter). From this count ...
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bioRxiv - Systems Biology 2020Quote: ... insoluble material was pelleted down at 2500 RCF for 10 minutes at 4C and the collected supernatant was subjected to ultracentrifugation (35000 RPM for 30 min. at 4C, Beckman-Coulter Optima Ultracentrifuge) to remove bacteria ...
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The Batten disease protein CLN3 is important for stress granules dynamics and translational activitybioRxiv - Molecular Biology 2022Quote: ... samples were layered onto a 10–50% sucrose gradient in lysis buffer and centrifuged in an SW40Ti rotor (Beckman Coulter, High Wycombe, UK) at 38,000 rpm for 2 h ...
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bioRxiv - Synthetic Biology 2022Quote: ... 20 μL reactions were prepared by dispensing 1 μL of each 10 μM reverse primer into the wells of a 96-well PCR plate using the Echo liquid handler (Beckman Coulter, Brea, CA). A mastermix consisting of polymerase premix ...
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bioRxiv - Biochemistry 2021Quote: ... was arrayed in a minimum of a triplicate across a 10 kDa MWCO 96-well microdialysis plate (SWISSCI Diaplate™) and sealed with aluminum foil seals (Beckman Coulter 538619) to create the protein chambers ...
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bioRxiv - Cell Biology 2020Quote: ... followed by serial centrifugation at 100’000 g for 120 min at 10 °C (Beckman fixed-angle TL110 rotor; Beckman Optima Max-TL ultracentrifuge). The so obtained P100 pellets ...
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bioRxiv - Bioengineering 2023Quote: ... the supernatant was then taken and loaded into a 10% to 45% sucrose gradient solution and centrifuged at 360000 rpm for 3 h (Beckman Optima XE-100). 18 fractions from each sample were collected using a piston gradient fractionator (Biocomp ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting lysate was loaded on top of 10 ml 29% iso-osmolar OptiPrep solution in a conical 30 ml centrifuge tube (Beckman Coulter, SW28 rotor). Samples were centrifuged at 10,100 × g (7,500 r.p.m. ...
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bioRxiv - Microbiology 2023Quote: MCP2201-LBD was diluted to 75 μM in SEC buffer with or without 10 mM ligand.Sedimentation velocity analytical ultracentrifugation experiments were performed on a Beckman XL-I analytical ultracentrifuge (Beckman Coulter, Brea, CA, USA) at 4 °C with a rotor speed of 60,000 rpm for 7 h ...
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bioRxiv - Cancer Biology 2023Quote: ... the supernatant was layered on top of a linear sucrose gradient (10–50% sucrose) loaded in a SW40 Ti swinging-bucket rotor (Beckman Coulter, Krefeld, Germany) and centrifuged (33.500 rpm/4°C/3h ...
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bioRxiv - Molecular Biology 2023Quote: ... aggregates and debris were removed by centrifuging at 1,000 ×g for 10 minutes at RT (Avanti J–15R; Beckman Coulter Inc., Brea, USA). Next ...
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bioRxiv - Cell Biology 2023Quote: ... either the dye was solubilized with 10% (v/v) acetic acid and the absorbance was measured at 570 nm in a spectrophotometer microplate reader (Beckman Coulter, Model AD340), or alternatively ...
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bioRxiv - Genomics 2020Quote: PCR products for each sample were pooled by targeted gene in equimolar ratios then each pool was 1:10 diluted and subsequently cleaned with 1 volume of AMPure XP (Beckman Coulter, Brea, California, USA) before quantification ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and supernatants were ultracentrifuged at 37 000 rpm (~100 000 g) and 10°C for 1h (Beckman Optima LE-80 K; rotor 70.1.Ti). Pellets were resuspended in 100μL of buffer (10 mM Tris-HCL ...
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bioRxiv - Microbiology 2021Quote: ... The pellet was resuspended in pre-warmed ddH2O (about 60 °C) and centrifuged at least 10 times in an ultracentrifuge (Beckman Coulter, Pasadena, California, USA), until residual SDS was completely removed ...
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bioRxiv - Plant Biology 2022Quote: ... Size selection of libraries was performed using 25 and then 10 μl of Agencourt AMPure XP beads (Beckman Coulter, Brea, CA, USA, Cat #A63880). All libraries were quantified with a Qubit fluorometer using the dsDNA high-sensitivity assay kit (Cat #Q32851 ...
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bioRxiv - Immunology 2024Quote: ... Unsupervised clustering of reduced clinical data was performed by FlowSOM (hierarchical consensus clustering; 25 clusters, 5 metaclusters, 10 iterations).81 All analyses were performed using Cytobank (Beckman Coulter Inc., Brea, CA).