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Citations for Beckman :
1501 - 1550 of 1603 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... was added before ultracentrifugation at 20,000 × g for 2 hours at 10°C in an Optima L-90K Ultracentrifuge with the SW32Ti rotor (Beckman Coulter). Supernatants were carefully discarded by aspiration and the 6 phage pellets were resuspended with 0.1 ml of TM buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: VLPs were collected after removal of cells from the enrichment culture (7,000 rpm, 20 min, Sorvall 1500 rotor) and concentrated by ultracentrifugation (40,000 rpm, 2h, 10°C, Beckman SW41 rotor). The particles were resuspended in buffer A ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were loaded onto 10-45% CHX supplemented sucrose gradients in 9/16 x 3.5 PA tubes (Beckman Coulter, #331372) and spun at 35,000 x g in SW41 for 3 hours at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... microbubbles rested for 10 min before the suspension was mixed and diluted to 0.2% v/v in Isoton (Beckman-Coulter). For each vial ...
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bioRxiv - Microbiology 2020Quote: ... diluted to 20 mL with PBS and pelleted through a cushion of 10% sucrose in PBS to further clean and concentrate the sample (SW32 Beckman centrifuge tube at 25,000 rpm at 4°C for 2 h) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The pellet was resuspended in 60% Percoll in STE and loaded on the bottom of six 10-35% Percoll gradient in STE in polycarbonate tubes for SW28 rotor (Beckman). Gradients were centrifuged for 1 hour at 24000 rpm ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with following antibody mix for 30 minutes on ice: pan-γδTCR-PE (1:10; IMMU510; Beckman Coultier) and CD69-APC (1:25 ...
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bioRxiv - Cancer Biology 2023Quote: ... The ribosomes with equal A260 in each group were loaded onto a sucrose gradient (10–50%) buffer prepared using a gradient station and centrifuged in an SW41Ti rotor (Beckman) at 4°C for 4 hours at 28,000 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... The density adjusted FBS was subjected to ultracentrifugation for 25 h at 310,500 x g at 10°C in a 70Ti rotor (Beckman Coulter) before dialysis ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were loaded onto 10-45% CHX supplemented sucrose gradients in 9/16 x 3.5 PA tubes (Beckman Coulter, #331372) and spun at 35,000 x g in an SW41 rotor for 3 hours at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... using a standard protocol but with all reaction volumes reduced by 1/10 to accommodate the automation of the prep on the Echo LabCyte liquid handler (Beckman). Libraries were purified using Agencourt Ampure XP beads (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Illumina 16S Metagenomic Sequencing Library Preparation Protocol with the following changes: 10 μL of Amplicon product was purified using a 1:0.8 ratio of XP beads (Beckman Coulter), with beads resuspended in a final volume of 10 μL of nuclease-free water ...
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bioRxiv - Genomics 2022Quote: ... Lysates were fractionated on a 10% to 60% sucrose gradient using the SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter) at 36,000 rpm for 3 h and 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... step gradients were made by superposing sucrose solutions of different concentration (50%, 40%, 30%, 20%, 10%) in an ultra-clear polycarbonate tube (Beckman), and a linear gradient was allowed to form overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... 7,500 GFP+ and 7,500 RFP+ single live cells were sorted into each well of a 24 well culture plate containing low pH DMEM with 10% FBS using a MoFlo Astrios cell sorter with Summit v6.3 software (Beckman Coulter). Sorting was performed at 25psi using a 100um nozzle ...
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bioRxiv - Immunology 2023Quote: ... at 950 rpm for 10 min at 20°C and centrifuged at 15,000 rpm (Beckman Coulter Microfuge® 22R refrigerated microcentrifuge, Beckman Coulter Australia Pty Ltd ...
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bioRxiv - Cancer Biology 2023Quote: Cell death was evaluated 48 hrs after ionizing radiation (10 Gy) by using a CytoFLEX S (Beckman Coulter, Milan, Italy) instrument ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein lysates were layered on top of the 10-30% glycerol gradients (10 mL) and fractionated by centrifugation at 40,000 rpm for 16 h using a SW32Ti rotor (Beckman Coulter). Fractions (20x ...
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bioRxiv - Microbiology 2024Quote: ... The remaining cells were pelleted using a 4,300 × g spin for 10 min in an Allegra X-14R centrifuge (Beckman Coulter) equipped with a swinging bucket SX4750 rotor ...
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bioRxiv - Immunology 2024Quote: ... was added, platelet-rich plasma generated by centrifugation (60xg, 7min, RT), platelets pelleted (240xg, 10 min, RT) and counted (Beckman Coulter Z1-D Dual Cell/Particle Counter) ...
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bioRxiv - Biochemistry 2024Quote: ... were added to the desired concentration before clarification by ultracentrifugation at 247,000 x (g) for 10 minutes at 2°C in a TLA100 (Beckman Coulter). Glucose oxidase (GmbH ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were pelleted at 280’000 x g for 10 min at 25 °C in an Optima XP centrifuge with a TLA-100 rotor (Beckman-Coulter). Supernatant and pellet fractions were separated on a 10 % SDS-PAGE gel and proteins were visualized by Coomassie Blue staining ...
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bioRxiv - Molecular Biology 2021Quote: ... About 1,5 mg of protein was loaded on a 10–50% sucrose gradient and centrifuged for 2h45min at 39 000 rpm in an Optima L-100XP Ultracentrifuge (Beckman-Coulter) using a SW41 rotor (Beckman) ...
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bioRxiv - Cell Biology 2022Quote: ... Yeast cells were stained with 5ug\ml propidium iodide for 10 minutes and samples were examined in CytoFlex flow cytometer (Beckman Coulter) using PE channel ...
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bioRxiv - Molecular Biology 2020Quote: ... and centrifuged through a 10–35% sucrose gradient for 19 h at 16,000 rpm in a SW28 rotor (Beckman Coulter, Inc.). Fractions containing the 70S peaks were pooled and kept at −80℃ for further use.
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bioRxiv - Cell Biology 2021Quote: ... The sample was overlaid with decreasing concentrations of Nycodenz (30%, 20%, 10% and 0%) and centrifuged in a SW55 rotor (Beckman Coulter) 2h 30 min at 55000 rpm and 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... Counting was performed twice for each sample (10 min per sample) with a ß-scintillation counter Beckman LS6000TA (Beckman Coulter, USA). Roots of two plants identically grown and processed but not exposed to the incubation solution were used to determine background counts ...
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bioRxiv - Microbiology 2020Quote: ... The resulting cDNA was PCR-amplified to about 10-20 ng/μl using a high-fidelity DNA polymerase followed by purification using the Agencourt AMPure XP kit (Beckman Coulter). The cDNA samples were pooled with ratios according to the RNA concentrations of the input samples and a size range of 200-550 bp was eluted from a preparative agarose gel ...
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bioRxiv - Cell Biology 2022Quote: ... which was then slowly poured on top of 10 ml cushion solution (2.05M sucrose with protease inhibitors, DTT and PMSF) in the ultracentrifugation tube (Beckman polyallomer #326823). After a 45-min spin at 24,600 rpm in pre-chilled SW32Ti rotor at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Putative LSC-enriched populations were isolated from freshly expanded MLL-AF9 mouse leukemia cells by sorting c-Kithigh (top 10%) cells on a MoFlo XDP cell sorter (Beckman Coulter). Sorted cells were plated in triplicate in semi-solid methylcellulose medium (Methocult M3434 ...
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bioRxiv - Neuroscience 2020Quote: ... the homogenized solution was added onto a sucrose cushion (2 ml) in a 10 ml Ultra-Clear centrifuge tube (Beckman Coulter). Separation of nuclei from the tissue was done by centrifugation (26.500xg at 4°C for 1.5 h ...
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bioRxiv - Genetics 2022Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase incubated for 4 hours at 37°C followed by inactivation with 1 μL of 10% SDS and purification using RNA Ampure XP beads (Beckman Coulter).
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bioRxiv - Immunology 2020Quote: ... cryopreserved PBMC were recovered overnight in RPMI1640 supplemented with 10% FBS and washed in cold phosphate buffer (PBS) before suspended in cold PBS and sorted on Moflo Astrios (Beckman Coulter). The excitation wavelength for the generation of side-scatter (488/6-nm filter ...
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bioRxiv - Molecular Biology 2020Quote: ... The assembled complexes were incubated for 5 min at 30 °C and for 10 min on ice before they were loaded on a 30% (w/v) sucrose cushion in TLA-100 tubes (Beckman Coulter). The sucrose cushions were centrifuged for 2 h at 390,880 xg and 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... The equivalent of 200 µl of cell extract with an OD260 of 12 was loaded onto a 10–50% sucrose gradient and centrifuged in a SW41 rotor (Beckman Coulter) at 39,000 rpm for 2.45 h at 4°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... The complete NanoTrap was purified using rate-zonal centrifugation33 through a 1545% glycerol gradient in 1×TE + 10 mM MgCl2 in an SW 55 rotor (Beckman Coulter). Fractions were collected after a 1 hr centrifugation at 50 k rpm ...
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bioRxiv - Biophysics 2021Quote: ... The proteoliposomes were mixed with 60% (w/v) sucrose and loaded at the bottom of a 10 ml polycarbonate centrifuge tube (Beckman Coulter). A discontinuous sucrose gradient was prepared (20% ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was centrifuged 10 min at 10 000×g and the supernatant centrifuged for 90 min at 95 000×g (Optima XE-90, Beckman Coulter). The pellet containing the membrane fraction was solubilized in 1% N-dodecyl-β-D-maltopyranoside (DDM ...
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bioRxiv - Microbiology 2021Quote: ... with a final extension at 72 °C for 10 min PCR products were purified using Agencourt AMPure XP beads (Beckman Coulter) ratio 0.6× ...
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bioRxiv - Microbiology 2023Quote: ... 1 μl or 10 μL of dilution were added to the 384-well plates using automated dispenser BioMek FX (Beckman Coulter). Extracellular bacteria were then eliminated by adding antibiotic to the media 1h after infection ...
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bioRxiv - Genomics 2023Quote: ... The parasites were then washed twice with an ice-cold WI buffer (UP03-10, Dojindo EU) and immediately analysed by CytoFLEX flow cytometer (Beckman Coulter). Non-GFP P ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatant was incubated in a boiling water bath for 5 min and centrifuged for 10 min at 40,000 rpm (Beckman Ti45 rotor). Supernatant was supplemented with ammonium sulfate (1 g per 6.1 ml) ...
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bioRxiv - Cell Biology 2024Quote: ... Microtubules and tau were incubated for 10 min at room temp and centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter). After centrifugation the supernatant was separated from the pellet and used as the sample indicated by the ‘tau in solution‘ ...
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bioRxiv - Neuroscience 2023Quote: ... The absorbance was recorded at 1 min intervals for 10 min at 340 nm at 30°C using a spectrophotometer (DU 800, Beckman Coulter). The enzymatic activity was determined as the change in absorbance/min/mg protein.
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bioRxiv - Molecular Biology 2023Quote: ... A 10-30% gradient of glycerol in HEMG buffer was poured into 14 x 89 mm polyallomer centrifuge tubes (Beckman #331372). The resuspended nuclear extract was carefully layered on top of the gradient and centrifuged in an SW41 rotor at 40,000 rpm for 16 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... A 12 ml 2 % step Optiprep gradient in LB ranging from 32 % to 10 % was prepared beforehand in Ultra-Clear tubes (Beckman Coulter). Samples were spun for 16 hours at 100000 g at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and EVs were detected using the 638 nm laser (optical filter 660/10 nm, APC) of a CytoFLEX LX Flow Cytometer (Beckman Coulter). For data acquisition CytExpert software version 2.1 was used ...
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bioRxiv - Cell Biology 2024Quote: ... 350 μL of Vangl1 positive fractions were diluted with 10 volumes of Tris buffer and pelleted in an ultracentrifuge equipped with a SW41Ti rotor (Beckman Instruments) at 25.000 rpm for 1 hour at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... The index PCR was stopped after 8–10 cycles and the library DNA was purified using AMPure XP (A63880, Beckman Coulter). The library was sequenced on an Illumina NovaSeq 6000 instrument (150-bp paired-ends ...
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Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... were prepared and cooled on ice. The 10-50% sucrose gradients (vol. = 12 mL) were formed in SW40 ultracentrifuge tubes (Beckman Coulter) using Gradient Station (Biocomp) ...