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Citations for Beckman :
1451 - 1500 of 1603 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Pellets were washed and gently resuspended in 1 mL of PBS and centrifuged at 50,000 x g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). Pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Bioengineering 2023Quote: ... subtilis spores were collected from the culture by the centrifugation at 8000 rpm at 4 °C for 20 min (Avanti J-E, Beckman Coulter Life Science, IN, USA). Supernatant was removed and the spore pellets were resuspended in fresh 100 mM PBS pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... Pellets were washed and gently resuspended in 1 ml of PBS and centrifuged at 50,000 × g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). The resulting pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Biophysics 2024Quote: ... The virus was pelleted from supernatants via centrifugation at 14000 rpm for 6 hours at 4°C using a Beckman J2-21 high-speed ultracentrifuge fitted with a JA14 rotor (Beckman Coulter Inc., Fullerton, CA, USA). After carefully removing supernatants ...
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bioRxiv - Genomics 2021Quote: ... Libraries were amplified for a total of 8-10 cycles and DNA purified using AMPure XP beads (Beckman Coulter). The quality and concentration of ATAC-seq libraries were evaluated using the Bioanalyzer DNA High Sensitivity Assay (Agilent Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... Lentivirus containing > 10 kb length insert (inducible CRISPRi and CRISPRa) was concentrated using AVANTI J-30I centrifuge (Beckman Coulter) with JS-24.38 swing rotor at 72000g for two hours at 4 degree ...
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bioRxiv - Cell Biology 2021Quote: ... with or without 5µM Gli for 15 minutes at room temperature in 1x BRB80 supplemented with 0.1 mg/mL BSA and then subjected to sedimentation by ultracentrifugation at 50,000 rpm for 10 minutes at 27°C in a TLA 120.1 rotor (Beckman Coulter). Pellets were subsequently resuspended and the amount of protein in each pellet and supernatant was analyzed by SDS-PAGE ...
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bioRxiv - Microbiology 2020Quote: ... and separated in potassium tartrate-glycerol gradient 10–35% for 2 hrs at 175,000 ×g (Beckman SW41 Ti rotor). Purified viral particles were suspended in 10 µg/ml BSA solution or 10 µg/ml CbAE-1 solution and incubated for 1 hr at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and 500 μl of a 10 ml resuspension of cells from each flask diluted with 19.5 ml Isoton II Diluent (Beckman). PD time ...
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bioRxiv - Cancer Biology 2020Quote: ... equivalent amounts of ribosomes for each lysate was loaded onto each 10%-45% sucrose density gradient (BioComp Gradient Master machine) for ultracentrifugation at 33,000 rpm for 5 hours (Beckman Optima L80K Ultracentrifuge ...
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bioRxiv - Biochemistry 2020Quote: ... the supernatant was mixed with GMPCPP-seeds from the prior step and then incubated at 37°C for 30 mins followed by centrifugation at 90,000 rpm for 10 min at 37°C (TLA120.1 Beckman Coulter). The microtubule pellets were rinsed twice with 100 μ! warm (37°C ...
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bioRxiv - Microbiology 2022Quote: ... phage suspension was layered on top of a 5–20 % sucrose (w/vol, in Tris-HCl pH 7.2 with 3 mM MgCl2 and 10 mM CaCl2) and centrifuged (Beckman Optima L-K90 ...
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bioRxiv - Microbiology 2022Quote: ... The light-scattering zone was collected and further purified in 20– 70 % sucrose gradient (w/vol, in Tris-HCl pH 7.2 with 3 mM MgCl2 and 10 mM CaCl2) by centrifugation (Beckman Optima L-K90 ...
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bioRxiv - Physiology 2022Quote: ... The dissected tissues were held in a glass-bottom dish (35 × 10 mm, IWAKI #3910-035) with an insect pin (φ0.10 mm, Ento Sphinx Insect Pins) and silicone grease (Beckman), and 20 μL of SDM was added to cover the tissue ...
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bioRxiv - Immunology 2021Quote: ... overlaid onto 5 ml of 10% sucrose in NTE (135 mM NaCl, 10mM TrisCl, ph 7.50, 1mM EDTA) in ultra-centrifuge tubes (Beckman) and centrifuged at 25,000 rpm for 90 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was loaded onto a 10-55% sucrose gradient followed by ultracentrifugation (Beckman Coulter Optima XPN-100 ultracentrifuge) at 32,500 × rpm at 4 °C for 80 minutes in the SW41 rotor ...
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bioRxiv - Systems Biology 2022Quote: ... The cell culture in a 48-well microplate (Iwaki) was suspended in 10 ml of ISOTON II (Beckman Coulter). Every 100 to 500 μl of the suspended solutions were flowed for particle analysis ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Linear 10–30% glycerol gradients were prepared in 11□mm□×□34□mm polyallomer centrifuge tubes (Beckman Coulter, Brea, CA) with HEM buffer [25 mM HEPES (pH 7.5) ...
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bioRxiv - Neuroscience 2022Quote: ... myelin was layered onto 3 mL ice cold 0.85 M sucrose solution in a 10 mL ultracentrifuge tube (Beckman) before centrifugation at 75 000 x g for 30 min at 4 °C on a Beckman Ultracentrifuge with MLA-55 rotor (acceleration 7 ...
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bioRxiv - Plant Biology 2023Quote: ... were added and incubated for 10 minutes at room temperature and purified with 0.4X AMPure XP beads (Beckman Coulter). 1D2 Adapters (ONT ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 mL of 30% and 1.8 mL 10% of iodixanol solutions and floated into the gradient by ultracentrifugation in a SW60Ti rotor (Beckman) at 192,000 x g ...
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bioRxiv - Developmental Biology 2023Quote: ... The cytosolic lysate was layered on 10%-50% sucrose gradients and centrifuged at 246,000 g for 65 min in SW55 swinging bucket rotor (Beckman). Continuous UV absorbance was monitored with Triax flowcell monitor (BioComp).
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bioRxiv - Plant Biology 2023Quote: ... were added and incubated for 10 minutes at room temperature and purified with 0.4X AMPure XP beads (Beckman Coulter). Adapter Mix II (ONT ...
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bioRxiv - Plant Biology 2023Quote: ... were added and incubated for 10 minutes at room temperature and purified with 0.5X AMPure XP beads (Beckman Coulter), and Long Fragment buffer (ONT ...
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bioRxiv - Plant Biology 2023Quote: ... were added and incubated for 10 minutes at room temperature and purified with 0.4X AMPure XP beads (Beckman Coulter) using Long Fragment buffer (ONT ...
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bioRxiv - Cell Biology 2022Quote: ... milk was prevented from cooling down and was processed within 20 minutes after collection by 2 rounds of centrifugation at 3,000xg for 10 minutes at 23°C (Beckman Coulter Allegra X-12R ...
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bioRxiv - Molecular Biology 2023Quote: ... 72 μL of 10% sarkosyl was added to 650 μL of each sample in ultra-centrifuge tubes (Beckman Coulter) and samples were rotated end-over-end at room temperature for one hour ...
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bioRxiv - Biophysics 2024Quote: ... Eluted GEMs were further analyzed by gradient sedimentation through a 10-60% glycerol gradient with a SW60 Ti (Beckman) rotor ...
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bioRxiv - Biochemistry 2024Quote: ... The crude extract was clarified by high-speed centrifugation (60 min at 14000 rpm at 10°C) by Beckman Coulter centrifuge ...
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bioRxiv - Genomics 2024Quote: ... 10 μg of resuspended DNA was subjected to a bead cleanup step (Genfind v3.0, Beckman Coulter, Brea, CA, USA) and 5 μg of DNA were used for HiFi library preparation ...
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bioRxiv - Molecular Biology 2024Quote: ... and the supernatant was loaded onto a 10-50% sucrose gradient followed by ultracentrifugation (Optima XPN-100, Beckman Coulter) at 1,90,000 x g (SW41Ti rotor ...
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bioRxiv - Neuroscience 2024Quote: ... Then 10 barcoded single mitochondria were pooled together and cleaned up by 1.5X AMpure XP beads (Beckman Coulter, A63881). 50 ng of the pool was input into a library using TruSeq Nano DNA kit (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... 10% and 0% in buffered sucrose on top of one another in 5 ml Ultra-Clear centrifuge tubes (Beckman). Gradients were centrifuged for 16 h at 100,000 x g and 4 °C ...
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Syntaxin-2 inhibits excessive phagocytic uptake but promotes phagolysosomal clearance in macrophagesbioRxiv - Cell Biology 2024Quote: ... followed by sequential topping with 2 mL each of 35, 25, and 10% sucrose (all prepared in 3 mM imidazole, pH 7.4) in SW40 tubes (344060, Beckman). The samples were then centrifuged at 100,000 g for 1 hr at 4°C using a SW40Ti rotor (Beckman) ...
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bioRxiv - Immunology 2024Quote: Phenotype and proliferation were evaluated with flow cytometry using Gallios 10-colour flow cytometer (Beckman Coulter, California, United States) or Cytoflex LX flow cytometer (Beckman Coulter ...
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bioRxiv - Neuroscience 2020Quote: ... for further depletion of cell debris and spun at 10,000 × g for 30 min at 4°C (AH-650 rotor, Beckman Ultra-Clear Tube with 5 ml capacity). The pellet was resuspended in 100 μl PBS and considered to be the “10K pellet” (10K or 10K fraction) ...
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bioRxiv - Immunology 2023Quote: ... UC-EVs were obtained by centrifuging 50 mL of conditioned media at 100,000 × g for 70 min at 4 °C (AH-629/36 rotor, Beckman Ultra-Clear Tubes with 36 ml capacity). Pellet was re-suspended in 250 µL of DPBS ...
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bioRxiv - Microbiology 2024Quote: ... The inner and outer membranes were precipitated through a first ultracentrifugation step (107 minutes at 55,000 rpm with a Beckman TLA-100.3 fixed-angle rotor, 4°C). To solubilize the inner membrane ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then resuspended in 0.1% BSA containing 10 µg/µl Hoechst 33258 and analyzed for senescence in a Cytoflex S cytometer (Beckman Coulter). For liver tissue ...
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bioRxiv - Cell Biology 2020Quote: ... they were mixed (total 160 μl), applied into a 10-mm path-length cell (105.201-QS, Hellma Analytics) in a DU720 spectrophotometer (Beckman Coulter), and then immediately measured with monitoring the optical density changes at 400 nm (ΔOD400 ...
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bioRxiv - Developmental Biology 2020Quote: ... at 37°C for 5 min and then fractionated on sucrose gradient (10-50 %) by ultracentrifugation at 39,000 rpm in a SW41-Ti rotor (Beckman coulter). RNA from monosome fraction was DNase treated and fragments of 28-30 nucleotides were size selected after running on a 15 % TBE-Urea gel ...
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bioRxiv - Cell Biology 2020Quote: ... and layered onto a continuous 10-50 w/w % sucrose gradient prepared in a 2.2 ml ultra-clear tube (11×34 mm, Beckman Coulter) using a two-step program in Gradient Master 108 machine ...
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bioRxiv - Molecular Biology 2020Quote: ... Debris was clarified by centrifuging at 18,000 ×g followed by fractionation on a discontinuous sucrose gradient (10-50 %) by ultracentrifugation at 39,000 rpm for 3 h in an SW41-Ti rotor (Beckman coulter). Monosome fractions were collected by pumping of Fluorinert FC-40 and using a fraction collector by measuring UV trace ...
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bioRxiv - Microbiology 2021Quote: ... by centrifugation at 4,000 x g for 10 minutes at room temperature in a tabletop centrifuge (Beckman Coulter, Pasadena, CA). Plasmid DNA was isolated from each cell pellet using the Purelink®HiPure Plasmid Maxiprep DNA Purification Kit (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... The experiments were carried out at 10 °C and 43,000 rpm in a XL-I analytical ultracentrifuge (Beckman-Coulter Inc.) equipped with both UV-VIS absorbance and Raleigh interference detection systems ...
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bioRxiv - Cell Biology 2021Quote: ... coli cells obtained from 500 mL culture were harvested using centrifugation for 10 min at 4800 rpm (Beckman, Allegra-30XR), and were subjected to freezing in liquid nitrogen ...
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bioRxiv - Microbiology 2021Quote: ... the wafer clump was collected using a pipette tip and resuspended in 3 mL of 2x Buffer C and mixed with 3 mL of 1x Buffer C (20 mM MES-Tris, 10 mM MgCl2, 50 mM KCl, pH 6.9) and pelleted in the ultracentrifuge (Beckman 50Ti). The resulting pellet was then resuspended in 200 µL of 1x Buffer C with 10% glycerol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Samples were centrifuged at 13,000 rpm (14,300 rcf) for 10 min using a Microfuge®18 Centrifuge (Beckman Coulter, USA). The supernatant was measured using a Zetasizer Nano ZS (Malvern Instruments Ltd. ...
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bioRxiv - Molecular Biology 2022Quote: ... Percoll in STE and loaded on six linear 10-35% Percoll gradients in STE in polycarbonate tubes for SW28 rotor (Beckman). Gradients were centrifuged for 1 h at 24,000 rpm ...
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bioRxiv - Immunology 2022Quote: ... for 30 min at 37°C and for 10 min at 80°C followed by a 1.2X AMPure XP beads (Beckman Coulter) cleanup ...