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Citations for Diagenode :
401 - 439 of 439 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... third instar larval wing discs were prepared by boiling samples for 10 minutes in Laemmli SDS-PAGE loading buffer followed by sonication using the Bioruptor Pico sonication system (Diagenode) for 10 cycles (30 sec on ...
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bioRxiv - Immunology 2023Quote: ... Cells were then lysed to isolate chromatin and sonicated for 10-20 min (depending on cell type) using a Bioruptor (Diagenode). Fragmentation of sonicated chromatin to lengths of 200-500 bp was confirmed by agarose gel electrophoresis.
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bioRxiv - Developmental Biology 2023Quote: ... Samples (∼200 µL) were then subjected to sonication (30 sec on/off cycles, 10 cycles) using a Bioruptor Pico sonication instrument (Diagenode). Whole cell lysates were cleared by centrifugation and protein concentrations were determined using the DC Protein Assay (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysis was obtained by pulsed ultrasonication (10 cycles, 30” On, 30” Off, at High intensity using Diagenode Bioruptor 300), complete lysis was monitored by phase contrast microscopy ...
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bioRxiv - Immunology 2023Quote: ... Nuclei preparation and shearing were performed as described previously53 with 10 cycles sonication (30s ON/30s OFF, Bioruptor Pico, Diagenode). PU.1 was immunoprecipitated overnight at 4°C using a polyclonal anti-PU.1 antibody (1:25 ...
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bioRxiv - Molecular Biology 2023Quote: Libraries were then prepared from 10 ng of RPFs using the D-Plex Small RNA-Seq kit for Illumina (Diagenode) and the D-Plex Unique Dual Indexes for Illumina – set A (Diagenode) ...
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bioRxiv - Immunology 2023Quote: ... or ChIP proceeded by sonicating with for 40 min (30 sec on/off 10 min cycles) in a Bioruptor UCD-200 (Diagenode). Lysates were cleared and 10% taken for input samples.
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bioRxiv - Neuroscience 2023Quote: ... At the last step of the protocol both nuclear and cytoplasmic samples were sonicated for 10 cycles (30 seconds on / 30 seconds off) using the Bioruptor Pico (Diagenode). For the preparation of the nuclear extraction and fractionation buffer the reagents used were HEPES (Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... 1x protease inhibitor) and sonicated for 10 cycles (sonication cycle: 30 sec ON, 30 sec OFF) using Bioruptor Plus (Diagenode). The samples were centrifugated at 14000x g for 10 minutes at 4°C and the supernatant containing the soluble chromatin was transferred to a fresh low-binding tube ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Lysis was performed by sonication (10-20 cycles of 30 seconds ON and 30 seconds OFF) in a Bioruptor Plus (Diagenode). For embryo collection ...
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bioRxiv - Neuroscience 2024Quote: ... The extracts were centrifuged for 10 min at 16,000g and supernatant was sonicated for 10 cycles with 30 sec on and 30 sec off per cycle on a Bioruptor Pico Sonicator (Diagenode). After sonication ...
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bioRxiv - Neuroscience 2024Quote: ... were subjected to 3 cycles of sonication (30sec ON/30sec OFF for 10 min, at 200W, high setting, Bioruptor, Diagenode). Triton X-100 was added to the sonicated lysate to a final concentration of 1% ...
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bioRxiv - Neuroscience 2024Quote: ... were subsequently subjected to 3 cycles of sonication (30s ON/30s OFF for 10 minutes at 200W, high setting, Bioruptor, Diagenode). Triton X-100 was added to a final concentration of 1% ...
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bioRxiv - Neuroscience 2024Quote: ... and sonicated for four cycles of 10 minutes (30 seconds ON 30 seconds OFF) at high setting using a Bioruptor Plus sonication device (Diagenode). The resultant fragmented chromatin was centrifuged at 10.000 g for 10 minutes and the supernatant was collected ...
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bioRxiv - Cell Biology 2024Quote: ... The extracts were centrifuged for 10 mins at 16,000g and the supernatant sonicated for 10 cycles (30 sec on, 30 sec off per cycle) on a Bioruptor Pico Sonicator (Diagenode). After sonication ...
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bioRxiv - Molecular Biology 2021Quote: ... A portion of sheared DNA (10%) was kept as input and the rest of the sheared DNA was immunoprecipitated with α-5-methylcytosine antibody (Diagenode), bound to magnetic beads ...
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bioRxiv - Microbiology 2020Quote: ... was added to the lysate and incubated at 37 °C for 10 min before sonication (2 × 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Microbiology 2022Quote: ... were added to the lysate and incubated at 37 °C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Biochemistry 2022Quote: ... heated to 95°C for 10 min shaking (1000 rpm) followed by 2 cycles à 20 s of sonication in BioRuptor (Diagenode). Aliquots containing 50 μg protein were transferred into a cartridge and cooled ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... The resuspended cell mixture was vortexed gently for 10 min and sonicated for five cycles of 30 s with a Bioraptor Plus (Diagenode SA) at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... the lysate was sonicated for 10 minutes (30 sec-on / 30 sec-off) in a Bioruptor instrument (Diagenode, Denville, NJ, USA) and then centrifuged at 15,000 rpm for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... The DNA was diluted to 100 µL at a concentration of 10 ng / µL and then sheared using a Biorupter® Pico (Diagenode) with 13 cycles of 30 s on followed by 30 s off and quantified using an Agilent D1000 ScreenTape (Agilent Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... were added to the lysate and incubated at 37°C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Genomics 2023Quote: ... Samples were sonicated for 10-12 minutes (high intensity, 30s on/30s off) in a Bioruptor Pico system (Diagenode, Denville, NJ) at 4°C ...
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bioRxiv - Genomics 2023Quote: ... A portion of sheared DNA (10 %) was kept as input and the rest of the sheared DNA was immunoprecipitated with α-5-methylcytosine antibody (Diagenode), bound to magnetic beads ...
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bioRxiv - Cell Biology 2022Quote: ... The suspension was incubated on ice for 10 minutes and vortexed briefly prior to sonication with a bath type sonicator (Diagenode, #B01020001). Sonication was performed on their high power setting at 4°C with five cycles of 30 seconds on and 30 seconds off ...
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bioRxiv - Cell Biology 2023Quote: ... a total of 10 ng of dsDNA was purified using the MicroPlex Library Preparation kit v3 (C05010001, Diagenode s.a., Seraing, Belgium), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then sonicated using a Bioruptor (60 sec ON/30 sec OFF, 10 cycles, high intensity at 20 °C) (Diagenode, Belgium), then heated to 95 °C for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... An input of 50 ng of genomic DNA was sheared to 6 kb - 10 kb using the Megaruptor® 3 (Diagenode) or g-TUBE (Covaris) ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were incubated on ice for 10 min and lysates were prepared by sonication in Bioruptor® Plus sonication device (Diagenode). The following steps were performed as indicated previously ...
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bioRxiv - Molecular Biology 2024Quote: ... the nuclear pellet was resuspended in 300 µl IP buffer and sonicated four times for 10 min (30 s on mode, 30 s off mode) using the Bioruptor Plus (Diagenode, Belgium). Sheared chromatin was collected by centrifugation and a 10-µg sample was used for IP ...
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bioRxiv - Microbiology 2021Quote: ... boiled at 95°C for 10 min and sonicated for 5 min using a Bioruptor® Plus sonication device (Diagenode, Liège, Belgium). Protein lysates were then run on a precast NuPAGE Bis-Tris 4–12% SDS–PAGE (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... cells were disrupted by sonication for 10 min (alternating 20 s off and 40 s off) using a Bioruptor Plus (Diagenode, Seraing, Belgium). Cell debris was removed by centrifugation at 14,000 rpm for 1 h at 4 °C and the supernatant was retained ...
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bioRxiv - Microbiology 2022Quote: ... was added to digest the proteins released by sonication during 10 minutes applying irradiation cycles of 30 seconds at the low irradiation setting (Bioruptor ultrasonicator, Diagenode, Lièges Belgique). Importantly ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were briefly cooled on ice and further sonicated for 10 min using 30 s on/30 s off iterations at maximum intensity in a Bioruptor ultrasonication device (Diagenode, Seraing, Belgium). Proteins were precipitated by the methanol/chloroform precipitation method (Wessel and Flügge ...
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bioRxiv - Neuroscience 2024Quote: Single snap-frozen soleus muscles derived from 3-month-old mice were pulverized at −80°C and proteins were extracted using 50 µl of a 4% SDS in PBS lysis buffer combined with 10 min sonication (30 seconds on, 30 seconds off) in a Bioruptor sonication bath (Diagenode, Denville, USA) at 14°C ...
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bioRxiv - Genomics 2024Quote: ... A total of 10 µg genomic DNA was sheared to a target fragment size of 19 kb using a Megaruptor 2 device (Diagenode, Denville, USA). In order to remove small fragments (<8kb) ...
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bioRxiv - Genomics 2021Quote: ... ChIPseq libraries were prepared from 10 ng of double-stranded purified DNA using the MicroPlex Library Preparation kit v2 (C05010014, Diagenode s.a., Seraing, Belgium), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% SDS and 0.5% N-lauroylsarcosine) and the chromatin was fragmented by sonication for 10 cycles on Bioruptor (Diagenode high settings, 30’’ on/off). Chromatin was separated from cell debris using centrifugation and stored at -80C for a maximum of 2 months.