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Citations for Diagenode :
1 - 50 of 439 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... at 4°C for 13 cycles with 30 sec on and 30 sec rest in 15 ml Bioruptor Tubes (Diagenode Cat #C30010017) with 650 mg of beads (Diagenode Cat #C03070001) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell suspensions in 0.6 mL µtube were sonicated in water bath at 4°C during 12 min (15 sec. ON, 15 sec. OFF) with a Bioruptor apparatus (Diagenode) setted on high power and then clarified by 10 min centrifigation at 10 000 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were extracted by sonication executing 40 cycles of 15s on and 15 s off at 4 °C using a Bioruptor plus (model UCD-300, Diagenode). After lysis ...
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bioRxiv - Genetics 2024Quote: ... Lysates were sonicated for 4×15 minutes using a Bioruptor UCD-200 (Diagenode). Hybridization of probes was performed for 16 hours ...
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bioRxiv - Biophysics 2024Quote: ... cells were lysed by sonication with 15 cycles (15 s on/10 s off) using the Bioruptor Plus (Diagenode) at 4C ...
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bioRxiv - Plant Biology 2020Quote: ... samples were placed on ice for 10 min and sonicated for 6 cycles 15 sec “on” – 15 sec “off” using Bioruptor Plus (Diagenode). After that ...
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bioRxiv - Genomics 2021Quote: ... After sonication (15 sec on/ 30 sec off, 5 cycles, 4 C) using Bioruptor 300 (Diagenode) 5 µl were stained with DAPI to verify isolation of intact Macs ...
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bioRxiv - Immunology 2022Quote: ... Sonicated chromatin was diluted ten times in ChIP Dilution Buffer and immunoprecipitation was done overnight at 4°C by incubating 2.5 μl (7 μg) of anti-H3K27ac antibody (C15410196; Diagenode) precoated on 25-μl protein A-coated magnetic beads (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... and sonication bath (3×10 sec at 4°C) (Bioruptor, Diagenode). Lysates were centrifuged (14.000x g ...
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bioRxiv - Molecular Biology 2021Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Molecular Biology 2024Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysis was carried out by sonication for 15 min (15s ON/15s OFF, medium intensity, Bioruptor, Diagenode) at 4°C in 100 µL/5000 tardigrades pellet of the following solution 12mM sodium deoxycholate ...
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bioRxiv - Genomics 2021Quote: ... sonicated for 10 cycles at 4°C using BioruptorPico device from Diagenode. For immunoprecipitation of H3K4me1 ...
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bioRxiv - Genomics 2021Quote: ... Lysates were sonicated for 15 cycles (30 sec on / 30 sec off) at 4°C using a Bioruptor Pico sonicator (Diagenode). After sonication ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were sonicated using 40 cycles of 15s on/off at 4°C in the Bioruptor plus (model UCD-300, Diagenode). Further ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were sonicated using 40 cycles of 15s on/off at 4 °C in the Bioruptor plus (model UCD-300, Diagenode) after boiling at 99 °C for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... The suspension was sonicated for 15 cycles (30 seconds on/ 30 seconds off) at 4 ◦C (Diagenode Pico Bioruptor sonicator). Chromatin concentration was quantified using the Qubit 4 Fluorometer and Qubit dsDNA Broad Range kit ...
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bioRxiv - Genomics 2023Quote: ... and 10 µg of DNA were sheared to appropriate size range (15-20 kb) using Megaruptor 3 (Diagenode). The sheared DNA was concentrated by bead purification using PB Beads (PacBio) ...
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bioRxiv - Genetics 2020Quote: ... Resuspended pellets were shaken for 10 minutes at 4°C and lysed by sonication in a 4°C water bath (Diagenode Biorupter) for five minutes (30 seconds on/30 seconds off) ...
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bioRxiv - Microbiology 2023Quote: ... 570 µM PMSF) for 10 min at 4 °C with sonication (Bioruptor, Diagenode, 10 min on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by sonication (13 cycles, 30 sec on, 1 min off, Diagenode). Approximately 25 μg of chromatin was immunoprecipitated with 2 μg of antibodies ...
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bioRxiv - Biochemistry 2022Quote: ... samples were sonicated at 4°C in 30 s on/off intervals for 15 cycles using a Bioruptor ® Plus sonication instrument (Diagenode) at high-intensity settings ...
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bioRxiv - Immunology 2022Quote: ... and was sonicated for 4 rounds of 15 cycles of 30 seconds on/30 seconds off on high power (Diagenode Biorupter plus). After sonication ...
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bioRxiv - Neuroscience 2024Quote: ... and sonicated for 15 min (cycles of 30 sec ON and 30 sec OFF, 4°C, low frequency) in a Bioruptor Pico (Diagenode, Seraing, Belgium). Samples were subsequently incubated for 15 min at 70°C ...
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bioRxiv - Immunology 2024Quote: ... by sonication for 15 min (30 s on/off cycles) at 4 °C with high power in a Bioruptor device (Diagenode, Liège, Belgium). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... The homogenized samples were submitted to 10 cycles of 10 s at maximal intensity at 4⁰C in a sonicator bath (Bioruptor, Diagenode, USA). Then the homogenates were centrifuged at 10,000 g for 10 min at 4⁰C and the supernatants (NP40-soluble fraction ...
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bioRxiv - Microbiology 2020Quote: ... supernatant aspirated and suspended in chromatin shearing buffer for sonication (10 cycles of 15 sec ‘on’ and 30 sec ‘off’) with a Bioruptor (Diagenode) to 200-900bp size ...
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bioRxiv - Cancer Biology 2022Quote: ... 10-15 cycles of sonication (30 sec ON, 30 sec OFF) were carried using the Bioruptor Pico sonicator device (Diagenode). H3k27ac antibody was obtained from Abcam (ab4729 ...
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bioRxiv - Cell Biology 2020Quote: ... the lysate was diluted 10 times with lysis buffer and subjected to sonication for 15 min in a refrigerated water bath (Bioruptor, Diagenode). Part of the sonicated chromatin was treated with proteinase K and RNase A and analyzed by electrophoresis in a 1.5% agarose gel to control the size of the DNA fragments (200-500 bp) ...
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bioRxiv - Genomics 2023Quote: ... Lysates were sonicated for 10-15 cycles of 30s ON/30s OFF on high power using the Bioruptor Plus (Diagenode), then diluted in additional lysis buffer 3 and clarified by centrifugation for 10 min at max speed at 4°C ...
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bioRxiv - Systems Biology 2024Quote: ... Afterwards the lysate was sonicated for 15 cycles of 30 sec ON/OFF at 10 °C using a Bioruptor (Diagenode) and subsequently frozen at -80 °C ...
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bioRxiv - Biophysics 2022Quote: ... samples were sonicated for 15 min (30 sec on, 30 sec off at 10°C) with a Bioruptor Pico (Diagenode). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-10 μg of gDNA were either fragmented to a size of 10-50kb with the Megaruptor 3 (Diagenode, E07010001 and E07010003) or fragmented to a size of 15-25kb with a gTUBE (Covaris ...
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bioRxiv - Cancer Biology 2024Quote: ... 4-10 μg of gDNA were either fragmented to a size of 10-50kb with the Megaruptor 3 (Diagenode, E07010001 and E07010003) or fragmented to a size of 15-25kb with a gTUBE (Covaris ...
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bioRxiv - Molecular Biology 2024Quote: ... All samples were lysed the same day 2 weeks before proteomics analysis in 100µL iST-NHS-Lysis buffer (PreOmics GmbH) by sonication (Bioruptor Diagenode, 15s ON/15s OFF for 15 min), and heating at 95°C for 10min ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated chromatin was fragmented by sonication at 4°C for 10 minutes using Bioruptor (Diagenode). Run-on reactions were performed at 37°C for 5 minutes using biotinylated nucleotides (5 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were incubated 10 minutes at 4°C and then sheared using a Bioruptor (Diagenode, Belgium) for 4 times at 5 cycles and followed by 2 cycles at 30″ ON/OFF high power ...
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bioRxiv - Genetics 2021Quote: ... incubated on ice for 10 min and subsequently sonicated at 4 °C with a Bioruptor sonicator (Diagenode) at high power for 5 cycles with 30 s ON and 30 s OFF ...
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bioRxiv - Molecular Biology 2023Quote: ... in 15 ml sonication tubes (Diagenode, C01020031) using 350 mg/mL of sonication beads (Diagenode ...
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bioRxiv - Microbiology 2023Quote: ... sonicated for 15 min with a Bioruptor (Diagenode) and centrifuged for 5 min at 16,000 × g at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by sonication for 13 cycles (30 sec on/off) at maximum intensity in a Bioruptor 300 (Diagenode). Samples were heated to 58 ºC for 5 mins in a Thermomixer ...
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bioRxiv - Neuroscience 2024Quote: ... The nuclear pellets were lysed with lysis buffer (50 mM Tris-HCl, 10 mM EDTA, 1% SDS, pH 8) and then sonicated with Bioruptor (Diagenode, 15 cycles, 30s ON/30s OFF). The size of the DNA in the sheared chromatin fragments was checked before precipitation by a Tapestation system (Agilent 4200 ...
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bioRxiv - Cancer Biology 2021Quote: ... lysed in 1% SDS containing buffer supplemented with 1x protease and phosphatase inhibitors, and sonicated for 4 x 10 cycles (30 sec on, 30 sec off) in temperature-controlled Bioruptor 300 (Diagenode). Debris was removed by centrifuging at 14,000 rpm at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... was then added at a final concentration of 1 % for 15 min at RT and samples were sonicated over 20 min (10 × 30 s pulses) at 4°C to cleave genomic DNA (Bioruptor, Diagenode). Anti-MAZ antibody was from Bethyl Laboratories (A301-652A) ...
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bioRxiv - Developmental Biology 2021Quote: ... Tubes were sonicated for 10 mins (sonication cycle: 30 sec ON, 30 sec OFF) at 4 °C in a Bioruptor ultrasonication bath (Diagenode) at high energy setting ...
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bioRxiv - Developmental Biology 2020Quote: ... Tubes were sonicated for 10 mins (sonication cycle: 30 sec ON, 30 sec OFF) at 4 °C in a Bioruptor ultrasonication bath (Diagenode) at high energy setting ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were sonicated at 4°C for 10 min (30 sec ON, 30 sec OFF) at medium frequency (Bioruptor Diagenode). Then ...
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bioRxiv - Cell Biology 2022Quote: ... cells were sonicated for 10 cycles of 30 sec “on” and 30 sec “off” at 4°C with Bioruptor (Diagenode) and the protein concentration was measured using Bradford protein assay (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 x 106 flow-sorted chromosomes were pelleted at 10,000 g for 10 min at 4 °C before tagmentation in a 50 μl reaction (2.5 μl Tagmentase (loaded Tn5 transposase, Diagenode, C01070012), 25 μl 2X Tagmentation buffer (Diagenode ...
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bioRxiv - Molecular Biology 2021Quote: ... The sample was resupended in 300μl water and sonicated for 13 cycles (30sec on/off) using a Bioruptor Pico (Diagenode) to achieve a fragment size of approximately 200bp ...