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Citations for Diagenode :
301 - 350 of 439 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... and 1 mM DTT) and sonicated for 3x 10 seconds using a Bioruptor Pico (Diagenode). Cells were then rotated at 4°C for 20 mins before the lysates were diluted with 500 µl of no salt buffer (50 mM Tris-HCl ...
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bioRxiv - Genomics 2020Quote: ... Chromatin was sheared 10 × 30 sec on/30 sec off with a Bioruptor Pico (Diagenode). Supernatant with chromatin extract was isolated after a 10 minutes centrifugation at 14.000g at 4°C ...
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bioRxiv - Physiology 2021Quote: ... 10% glycerol) supplemented with protease and phosphatase inhibitor cocktails and sonicated in a Bioruptor (Diagenode). Protein lysates were quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific ...
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bioRxiv - Genomics 2022Quote: ... and fragmented by a Bioruptor Pico (10 cycles, 30 sec on/30 sec off; Diagenode). The sonicated solution was diluted (1:5 ratio ...
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bioRxiv - Genomics 2023Quote: DNA was sheared to an appropriate size range (10-20 kb) using Megaruptor 3 (Diagenode) followed by Ampure bead purification ...
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bioRxiv - Genomics 2023Quote: DNA was sheared to an appropriate size range (10-20 kb) using Megaruptor 3 (Diagenode) followed by SMRTbell cleanup beads ...
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bioRxiv - Immunology 2024Quote: ... Samples were boiled for 10 min at 95 °C and sonicated in a Bioruptor (Diagenode) for 10 cycles of 30 sec on/30 sec off ...
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bioRxiv - Plant Biology 2024Quote: ... Sheared chromatin (10 cycles, 30 s ON/ 30 s OFF using a Bioruptor® (Diagenode)) extracted from 100 mg material was equally divided between input and samples immunoprecipitated with anti-histone H3 (Merc ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysis and nuclei isolation were carried out in 10 mL of LB1 buffer (Diagenode) by rotating 20 min at 4 C ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was then de-crosslinked and eluted for 4 hours at 65C before being purified using IPure beads (Diagenode Cat# C03010014). The purified DNA was then subjected to sequencing library preparation or qPCR analysis ...
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bioRxiv - Plant Biology 2021Quote: ... The IP of the methylated DNA was performed overnight at 4°C using Protein A Dynabeads coated with anti-5mC (Diagenode, C15200081) or anti-IgG (Diagenode ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were incubated by rotating overnight at 4°C with primary antibody or irrelevant IgGs and then incubated with protein-A agarose beads (Diagenode; C03020002) previously blocked with 0.5% BSA for 3 hr at 4 °C with rotation ...
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bioRxiv - Systems Biology 2021Quote: ... 750 U/µl Sm DNAse) and sonicated (5 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). HA or Streptactin beads were added to cleared lysates and samples were incubated for 3 hours at 4°C under constant rotation ...
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bioRxiv - Systems Biology 2021Quote: ... 750 U/µl Sm DNAse) and sonicated (5 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). Following normalization of protein concentrations of cleared lysates ...
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bioRxiv - Genomics 2021Quote: ... pH 7.4) aided by homogenization by passing the cells through various gouge needles and sonication using the Bioruptor® sonication system (Diagenode) for 15 cycles of 30 s ON / 30 s OFF at high setting ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: Membranes disruption was carried by sonication at 4 °C (2x10 min, 30 sec on, 30 sec off, high setting, Diagenode Bioruptor). Extracts were cleared by centrifugation (15,000 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... for a total volume of 100 μL and sonicated (4 cycles of 20sec/30sec (on/off time)) using a Bioruptor (Pico II, Diagenode, BE). The successful generation of fragments with a mean fragment size of 400 nucleotides was confirmed using a Bioanalyzer as per manufacturer’s instructions (Agilent ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196; H3K4me3, Diagenode 3C15410003). Reverse-crossed IP sample were pulled down with streptavidin C1 beads (Life Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... RNA samples were incubated overnight at 4°C with 12.5 µg of anti-5-hmC antibody (Diagenode rat monoclonal MAb-633HMC) or without antibody as negative control in IP buffer (750 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: Immunoprecipitation was performed using the IP star robot at 4°C (indirect 200 µl method, SX-8G IP-Star® Compact Automated System, Diagenode) with an anti-BrdU antibody (10 μg ...
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bioRxiv - Microbiology 2021Quote: 60,000 iATII cells or Calu-3 cells were incubated for 1 h at 4°C with equal protein concentrations of control rabbit IgG (Diagenode, Cat#C15410206) or 1/200 dilution of rabbit anti-ACE2 (Abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... were then incubated overnight at 4°C using primary antibodies for H3K27ac and SET in iC buffer from iDeal ChIP-seq kit for Histones (Diagenode, cod. C01010051). The next day ...
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bioRxiv - Plant Biology 2023Quote: ... Slides were blocked with 4% BSA for 30 minutes at 37°C in a moist box followed by primary (anti-H3K9m2, C15410060, Diagenode, 1:500) and secondary (Alexa Fluor 488 Goat anti-Rabbit IgG1 Secondary Antibody ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg total RNA was incubated overnight at 4 °C with Dynabeads™ Protein G magnetic beads pre-bound with anti-Ψ (Diagenode) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... followed by sonication at high power for 10 cycles of 30s ON/OFF (Bioruptor Plus; Diagenode). Lysates were clarified by centrifugation at 10,000g for 15min ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were fixed for 10 minutes at RT in 11% formaldehyde solution in fixation buffer (Diagenode), diluted 1:10 in RPMI with 10% FBS ...
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bioRxiv - Developmental Biology 2020Quote: ... split into two Eppendorf tubes and sonicated for 10 minutes in a Bioruptor® Pico (Diagenode) under standard conditions (30s on ...
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bioRxiv - Physiology 2021Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of antibody (H3K4me3 (Diagenode C15410003), H3K4me1 (abcam ab8895 and H3K27ac (Diagenode C15410196) ...
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bioRxiv - Cell Biology 2022Quote: ... The lysates were sonicated (30 s/30 s, 10 cycles, high performance) by Bioruptor (Diagenode; B01020001), followed by centrifugation at 21000xg for 20 min at 20°C ...
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bioRxiv - Plant Biology 2022Quote: ... 50 μM MG132) were added and the samples were sonicated for 10 min on Bioruptor (Diagenode) at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10 ng sheared to an average fragment size of ∼300 bp (Diagenode Bioruptor Pico; B01060010) for enzymatic conversion / library preparation ...
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bioRxiv - Neuroscience 2024Quote: ... The lysates were then sonicated with 3 × 10 cycles (30 s on and off) (Bioruptor, Diagenode) to generate chromatin fragments of ∼500 bp in length ...
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bioRxiv - Neuroscience 2023Quote: ... then sonicated for 10 cycles of 30 s each on/off in a Bioruptor plus (Diagenode) in presence of 50 mg Protein Extraction beads (C20000021 ...
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bioRxiv - Cell Biology 2023Quote: ... for 10 cycles 30 sec ON/30 sec OFF in 1.5 mL Diagenode tubes (Diagenode; #C30010016) to generate 100–400 bp DNA fragments ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated on ice for 10 minutes and sonicated using Bioruptor Pico sonicator (Diagenode) for 23 cycles (30s ON/30s OFF) ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼10-20 million cells per shearing tube were sheared by sonication using either the Bioruptor (Diagenode UCD-200 ...
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bioRxiv - Microbiology 2024Quote: ... The cells were sonicated (10 cycles, 30 sec high-power pulse) using a Bioruptor Plus (Diagenode) and centrifuged (10,000 x g ...
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bioRxiv - Genomics 2024Quote: ... USA) with 10 μg DNA sheared using the Megaruptor 2 system (Diagenode, Liège Science Park, Belgium) to obtain an average fragment size of 15-20 kb ...
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bioRxiv - Cell Biology 2024Quote: ... sonicated at high performance for 10 cycles (30 s on/off) using Bioruptor Plus Ultrasonicator (Diagenode), and spun down for 20 minutes at room temperature at 20000 g ...
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bioRxiv - Immunology 2024Quote: ... We transferred cells into 1.5 ml sonication tubes (200 µL/tube) and sonicated 30 sec on/30 sec off for 6 cycles at 4 °C using Bioruptor® Pico (Diagenode, Denville, NJ). We centrifuged fragmented chromatin at 20,800 g for 10 min at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting chromatin was incubated overnight at 4°C with histone modification antibodies (H3K27ac, Abcam ab4729; H3K27me3, Diagenode C15410195 and H3, Abcam ab1791) pre-conjugated with Dynabeads Protein G ...
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bioRxiv - Developmental Biology 2024Quote: ... 1% PIC) and sheared to 100-600bp fragments (average 250bp) at 4°C in a Bioruptor® Pico sonication device (Diagenode: Denville, NJ) for 5 cycles (30 sec on ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclear extracts were collected and sonicated for 10 minutes to obtain 300bp chromatin fragments (Diagenode Bioruptor Pico). Equal volumes of sheared chromatin were immunoprecipitated with rabbit AR-V7 antibody (RevMab 31-1109-00) ...
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bioRxiv - Neuroscience 2021Quote: ... and chromatin was sheared using a Bioruptor Pico sonicator (10 sonication cycles 30’’ ON/ 30’’ OFF, Diagenode). Sheared chromatin was incubated under constant rotation at 4°C O/N with Protein A-coated magnetic beads ...
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bioRxiv - Physiology 2022Quote: ... Chromatin from approximately 10 million cells was sheared using a sonication device (Bioruptor Pico, #2013-2019, Diagenode) and optimized to produce ~400bp fragments ...
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bioRxiv - Genomics 2022Quote: ... 10 μg of DNA was purified then sheared to 20 kb fragments using the megaruptor1 system (Diagenode). For this library ...
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bioRxiv - Genomics 2021Quote: ... Cells were then sonicated for 10 cycles of 30 seconds on/off on a Bioruptor Pico (Diagenode) at 4°C ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of human antibody (H3K4me3 [RRID:AB_2616052, Diagenode C15410003] ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin from 10 million cells was sonicated to between 200-600 bp using a Bioruptor Plus (Diagenode). 100µL of Dynabeads Protein G (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were sonicated for 10 cycles (30 seconds on / 30 seconds off) using the Bioruptor Pico (Diagenode), followed by a centrifugation at 4°C for 20 min at 14000g ...