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Citations for Diagenode :
351 - 400 of 439 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... lysates were harvested in 1X RIPA buffer with 10% SDS and sonicated with a Bioruptor Plus (Diagenode). Protein was separated on NuPAGE 4-12% Bis-Tris gels (Invitrogen NP0336) ...
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bioRxiv - Genomics 2024Quote: Approximately 10 µg of genomic DNA was sheared to 30-50 kb using the Megaruptor 3 (Diagenode). The sheared DNA was treated with exonuclease to remove single-stranded ends ...
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bioRxiv - Genetics 2024Quote: ... The mixture was heated to 95°C for 10 minutes and sonicated in a Bioruptor (Diagenode, Belgium) (30 seconds sonication ...
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bioRxiv - Genomics 2024Quote: ... DNA was sheared to 10-20 kb with a Megaruptor 3 sonicator (Diagenode, Denville, New Jersey, USA) and treated with SMRTbell cleanup beads (PacBio ...
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bioRxiv - Evolutionary Biology 2023Quote: ... a maximum of 600 ng of DNA per accession was sheared at 4°C to a target average length of 400 bp using a Bioruptor® Pico sonication device (Diagenode, Denville, NJ, USA). Illumina libraries were prepared using a modified KAPA protocol with the KAPA LTP Library Preparation Kit (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... boiled at 95°C for 10min and then DNA was sheared by sonication (Bioruptor® Pico, Diagenode; 4 cycles of 30s ON + 90s OFF). In both cases ...
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bioRxiv - Molecular Biology 2021Quote: ... sample DNA was ethanol precipitated and sheared in 500 µL sonication buffer (50 mM Tris pH 8.0, 0.1% SDS, 10 mM EDTA) on a Bioruptor Pico sonicator (Diagenode). DNA was then concentrated on Amicon ultra 0.5 30K filter units (Millipore) ...
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bioRxiv - Genomics 2021Quote: ... A total of 10 μg of DNA was purified then sheared at 40kb using the Megaruptor system (Diagenode). Using SMRTBell template Prep Kit 1.0 (PacBio) ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were sheared by 10 cycles of sonication (30s on and 30 s off) in a Bioruptor (Diagenode) followed by centrifugation at 12K rpm for 10 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were sonicated for 10 minutes using a Bioruptor device together with protein extraction beads (Diagenode, Denville, NJ). Each lysate was diluted with 1080µl 50mM Tris pH7.4 ...
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bioRxiv - Genomics 2023Quote: 10 ug of C5 genomic DNA was sheared to 30 kb - 50 kb using the Megaruptor 3 (Diagenode). The sheared DNA was treated with exonuclease to remove single-stranded ends ...
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bioRxiv - Cell Biology 2023Quote: ... then fragmented by sonication (10 cycles of 30 sec ‘on’ and 30 sec ‘off’) using Bioruptor Pico (Diagenode). Sheared chromatin was cleared by centrifugation ...
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bioRxiv - Neuroscience 2024Quote: ... protein lysates were sonicated for 10 cycles (30 seconds on / 30 seconds off) using the Bioruptor Pico (Diagenode). Samples were centrifuged at 4°C for 30 minutes at 14,000g ...
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bioRxiv - Genomics 2024Quote: ... HMW DNA was sheared to an appropriate size range (∼10-30 kb) using a Megaruptor 3 (Diagenode, USA). The sequencing library was constructed using the SMRTbell Express Template Prep Kit 2.0 following manufacturer’s protocols ...
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bioRxiv - Neuroscience 2024Quote: ... Thawed samples were sonicated (2 cycles [10 s], 30 s between cycles, high setting; Bioruptor Next Gen, Diagenode) and heated (+90 °C for 7 min) ...
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bioRxiv - Cell Biology 2024Quote: ... and 4U/mL RNAse, and sonicated for 10 min (30’’ON/30’’OFF, max) using Bioruptor Plus (Diagenode). Lysates were cleared by centrifugation at 20,000 g at 4°C for 30 minutes ...
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Deep learning the collisional cross sections of the peptide universe from a million training samplesbioRxiv - Systems Biology 2020Quote: ... The samples were boiled at 95°C for 10 min and subsequently sonicated at maximum power (Bioruptor, Diagenode, Belgium). Proteolytic digestion was performed overnight at 37 °C by adding either i ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in 100 μL resuspension buffer (50 mM HEPES-NaOH pH 7.2, 150 mM KCl) and lysed for 10 minutes using the Bioruptor (Diagenode, 10 cycles ...
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bioRxiv - Genomics 2021Quote: ... Libraries were prepared from 10 ng of double-stranded purified DNA using the MicroPlex Library Preparation kit v2 (Diagenode) following the manufacturer’s protocol with some modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... which where lysed in a high-salt buffer (50mM Tris, 600mM NaCl, 10% glycerol, 0.2% NP40, 0.5mM DTT) followed by sonication to fragment chromatin (Diagenode Bioruptor). Buffers were supplemented with phosphatase and Complete ULTRA protease inhibitor cocktails (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were harvested in lysis buffer containing 0.1% Rapigest and sonicated (10 cycles of 30 seconds on, 30 seconds off in Bioruper, Diagenode). Approximately 30 μg of protein was resolved on a 4-20% Miniprotean TGX gel ...
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bioRxiv - Immunology 2020Quote: ... The cells were cross-linked with 1% formaldehyde for 10 minutes at RT and quenched with 2.5 M glycine (Diagenode) for 5 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: ... The DNA was sheared to a modal size of 10 kb using a Megaruptor 2 (Diagenode Diagnostics, Seraing (Ougrée), Belgium) ...
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bioRxiv - Cancer Biology 2024Quote: ... Crosslinking was terminated by the addition of 1/10 volume 1.25 M glycine for 5 min at room temperature followed by cell lysis and sonication (Bioruptor, Diagenode), resulting in an average chromatin fragment size of 200 bp ...
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bioRxiv - Genomics 2023Quote: ... Sonicate tissue using Diagenode Bioruptor Pico (10 cycles; 30 seconds on/ 30 seconds off) using 1.5mL microtubes (Diagenode C30010016) with 300uL per tube (several rounds needed) ...
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bioRxiv - Cell Biology 2023Quote: ... samples were not treated with benzonase during chromatin solubilization and sonication was carried out for 10 min (Diagenode Biorupter) as in 44 ...
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bioRxiv - Genomics 2024Quote: ... 5 μl of each annealed A and B transposome were combined with 10 μl of unloaded Tagmentase (Diagenode, C01070010), briefly vortexed ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were sonicated for 30 minutes at 4°C (30 seconds ON, 30 seconds OFF, output setting high) using a water bath sonicator (Diagenode Bioruptor® Plus; Cat. No. B01020001), transferred to new 1.5 ml tubes ...
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bioRxiv - Plant Biology 2020Quote: ... Nuclei were disrupted by sonication 10 times for 30 s each and 30 s of cooling in a Bioruptor (Diagenode). IPs were performed using α-GFP (Ab6556 ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was kept on ice and the pellet was resuspended in 200 µL OLB with 500 mM NaCl and sonicated for 10 min at high setting with 30 s / 30 s duty cycle on a Bioruptor sonicator (Diagenode) and centrifuged for 5 min at 20,000 × g at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were sonicated for 10 min at the high intensity setting (30 sec on/off) in a Bioruptor (Diagenode). The whole cell extract was adjusted to 25 mM MgCl2 and 5 mM CaCl2 and 1 µL DNAse I (30 U ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was sheared by sonication for 10 min at high settings (30 s on, 30 s off) using a Bioruptor Pico sonicator (Diagenode) to reduce sample viscosity ...
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bioRxiv - Genomics 2021Quote: ... we obtained 5 μg of high molecular weight genomic DNA (> 50% of fragments ≥ 40 kb) and sheared fragments to ~10 kb using the Megaruptor 3 (B06010003; Diagenode), followed by DNA repair and ligation of PacBio adapters using the SMRTbell Template Prep Kit v1.0 (100-991-900) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM TCEP was added and the pellet was sonicated in a Bioruptor Pico cooled by Minichiller 300 (both Diagenode). Chloroacetamide was added to a final concentration of 15 mM and samples were incubated for 10 min at 95°C with gentle agitation ...
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bioRxiv - Developmental Biology 2022Quote: ... in VFG media for 10 minutes at room temperature followed by a 5-minute quench with glycine (in True MicroChIP kit, Diagenode) at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Genomics 2022Quote: ... 20 mM Na Butyrate) and sonicated for 30 sec ON/OFF for 10 min in a Bioruptor®Pico (Diagenode) to generate 200-500 bp DNA fragments ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were incubated on ice for 10 minutes followed by sonication 5x for 30 sec on/off on high using a Bioruptor (Diagenode). Lysates were then spun at 10,000 x g for 10 minutes at 4°C ...
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bioRxiv - Genetics 2020Quote: S2 cells transfected with either mCherry-Nnk or Odj-mCherry for 72 hours were fixed with 1% paraformaldehyde for 10 minutes and sheared with Bioruptor sonicator (Diagenode) to obtain chromatin ...
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bioRxiv - Neuroscience 2020Quote: PFF transduction was conducted at 10 DIV by adding 2 ug/ml (96 well plate and coverslips) or 10 ug/ml (biochemistry) of PFFs diluted in PBS and sonicated with a BioRuptor (Diagenode) bath sonicator for 10 cycles of 30 seconds on ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were cross-linked for 10 min at room temperature with 1% formaldehyde before lysis followed by sonication of their chromatin (Bioruptor Plus, Diagenode) to obtain DNA fragments of 200–400 bp ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... To improve the proportion of DNA fragments at the optimal length for library preparation samples were sonicated for 10 cycles using the Bioruptor Pico Sonication Device (Diagenode). FFPE DNA samples were treated with the NEBNext FFPE DNA Repair Mix (NEB ...
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bioRxiv - Genomics 2020Quote: ... nuclei were isolated via sonication using a Bioruptor Pico (Diagenode; 9 cycles of 10 sec on and 30 sec off). Chromatin was then sheared in the recommended shearing buffer (27-30 cycles ...
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bioRxiv - Microbiology 2022Quote: ... Immunoprecipitation was done on chromatin corresponding to the remaining 10 000 cells using antibodies against H3K4me3 (Diagenode, cat no. C15410003) – 10% of the chromatin was used for input ...
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bioRxiv - Molecular Biology 2023Quote: ... and sonicated for 10 min (medium intensity, 30 s on/30 s off) in a Bioruptor UCD-200 system (Diagenode), and centrifuged at 21,000 x g for 5 min ...
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bioRxiv - Plant Biology 2023Quote: ... Nuclei were lysed in NLB (50 mM Tris-HCl pH8, 10 mM EDTA, 1% SDS and 1x Proteases Inhibitor cocktail. Chromatin was sonicated using a Diagenode Bioruptor (40 Hz ...
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bioRxiv - Immunology 2022Quote: ... with a freshly added protease inhibitor cocktail and sodium vanadate and subjected to 9 cycles (30s ON-10s OFF) of ultrasonication (Bioruptor Plus; Diagenode). The sonicated samples are then spun down ...
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bioRxiv - Neuroscience 2022Quote: ... A modified protocol for on-beads digest (sonication for 10*30s in a Bioruptor sonicator in presence of 50 mg Protein Extraction beads, C20000021, Diagenode) was used for tissue samples followed by a 3.5h digestion step ...
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bioRxiv - Molecular Biology 2022Quote: ... Protease inhibitor and RNase inhibitor) and sonicated for 10 cycles (30 secs on 30 secs off) on a Bioruptor (Diagenode) sonicator ...