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Citations for Diagenode :
201 - 250 of 439 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... boiled for 5 min at 95°C 1000rpm in 2% SDC buffer (2% SDC, 100 mM Tris-HCl pH=8.5) and sonicated (Diagenode Bioruptor, 15 * 30s at high intensity). Protein concentration was determined via BCA Protein Assay (Thermo ...
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bioRxiv - Neuroscience 2024Quote: ... PFFs were sonicated on high power (10 cycles of 30 sec on and 30 sec off) at 10°C with the Diagenode Bioruptor Plus (Diagenode B01020001, Denville, NJ, USA) prior to use.
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... 30 µg of chromatin and 4 μl of mouse anti-ERalpha monoclonal antibody (C15100066, Diagenode) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... For each sample the Ab coating mix was done with 4 μl anti-H3K4me3 (Diagenode, Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... with following appropriate antibodies at 4 °C o/n a rotating wheel: H3K27me3 (Diagenode, C15410195), RING1B (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... and then sonicated for 4 minutes (30 sec on / off) using a Biorruptor Pico (Diagenode). Protein concentrations were determined by the BCA method (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 s on / 30 s off) at 4°C using a water bath-sonicator (Diagenode). Chromatin fragments were reverse cross-linked overnight at 65°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were kept on ice for one hour before sonication at 4°C (Bioruptor, Diagenode) to an average DNA length of 200–500 bp by 15 pulses of 10 sec on and 10 sec off at high intensity ...
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bioRxiv - Molecular Biology 2023Quote: ... Unloaded Tn5 enzyme can be purchased from Diagenode (Cat:C01070010-10) and all sequences used for loading Tn5 are contained in Supp ...
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bioRxiv - Evolutionary Biology 2023Quote: ... then sheared at 10 kb using the Megaruptor1 system (Diagenode). Using SMRTBell template Prep Kit 1.0 and SMRTbell Barcoded Adaptater kit 8A or 8B kits (PacBio) ...
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bioRxiv - Cell Biology 2023Quote: ... by sonication (Bioruptor, 10 cycles, 30s on/off, Diagenode, Belgium). Proteins were subsequently reduced by 10min incubation at 95°C and alkylated with 10 mM chloroacetamide for 30min at 37 °C ...
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bioRxiv - Bioengineering 2024Quote: Unloaded Tn5 transposase proteins were acquired from Diagenode (#C01070010-10). Tn5 transposase was then loaded with oligos containing mosaic ends incorporating 13 base-pair unique molecular identifiers (UMIs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA concentration was normalized (except for low quality samples) and mechanically fragmented to 300-600 bp fragments using a Bioruptor Pico (Diagenode; 15s ON, 90s OFF, eight cycles). Low quality DNA were then reconcentred with AMPure XP beads and libraries were constructed according to the NEBNext® Ultra II DNA Library Prep for Illumina® kit recommendations (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... shear DNA and enhance cell disruption by using a water-bath (4°C) sonicator Bioruptor (Diagenode) at High Power for 40 min (sonication cycle ...
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bioRxiv - Genomics 2022Quote: ... Cell lysates were then sonicated 20 times at 4°C (Bioruptor Plus, Diagenode, 30/30 cycles) and centrifuged at 14000 rpm for 1 minute at 4°C to remove cellular debris ...
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bioRxiv - Molecular Biology 2022Quote: ... After sonication for 5 cycles of 30 seconds ON/OFF at 4 °C (Bioruptor Pico, Diagenode), the mixture was centrifuged (13 000xg ...
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bioRxiv - Physiology 2023Quote: ... 500µl volume) in a water bath sonicator set at 4°C (Bioruptor 300; Diagenode, Denville, NJ). After centrifuging at 10000g for 10 minutes at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... sonicated with Biorupter® Sonicator for 10 cycles (Cat#B01020001, Diagenode), and centrifuged at 13,000 rpm for 10 min ...
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bioRxiv - Immunology 2021Quote: ... 10 mM EDTA (pH 8.0)) and sonicated in TPX microtubes (Diagenode) in a 4°C-cooled water bath sonicator (Diagenode Bioruptor ...
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bioRxiv - Genomics 2020Quote: ... and 1 EDTA-free protease inhibitor tablet (Roche)/10 mL in H2O) before sonication with a Bioruptor Pico (Diagenode) for 5 minutes (30 seconds on ...
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bioRxiv - Neuroscience 2020Quote: ... medium intensity in a bath sonicator at 10 C (BioRuptor; Diagenode) and then rotated at 4 C for 20 minutes ...
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bioRxiv - Physiology 2020Quote: ... by sonication (Bioruptor, 10 cycles, 30 seconds on/off, Diagenode, Belgium) and proteins were digested as described previously (Ahrne et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... Sonication (10 cycles, 30sec on/off) was performed with Picoruptor (Diagenode) according to the Diagenode recommendation ...
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bioRxiv - Genomics 2023Quote: ... The samples were fragmented to 10 kb using Megaruptor 2 (Diagenode). For each fragmented sample ...
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bioRxiv - Cancer Biology 2023Quote: ... by sonication (Bioruptor, 10 cycles, 30 seconds on/off, Diagenode, Belgium) and proteins were digested as described previously70 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were sonicated for 10 cycles with Bioruptor Pico (Diagenode) until chromatin was sheared to an average length of 200 bp ...
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bioRxiv - Developmental Biology 2023Quote: ... sonicated for 10 cycles in a Bioruptor Pico™ sonicator (Diagenode) and diluted 1:1.5 ratio in equilibrium buffer (10mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were sonicated for 10 cycles (30s on/ 30s off; Diagenode). Protein concentration was measured using the BCA assay (Thermo Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... and RISC-Poor small sequencing libraries were constructed with CATS RNA-seq kit (Diagenode C05010041 Lot#4). Library primer dimers were removed by AMPureXP beads (Beckman) ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequently disrupted by 2 × 20” high intensity sonication cycles at 4°C in a BioRuptor (Diagenode). Protein concentration was determined using the Micro BCA™ protein assay kit according to manufacturer’s instructions (Thermo Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were disrupted by sonication (two cycles à 20 s at 4 °C) in a BioRuptor (Diagenode), and extracts were cleared by centrifugation at 15,000 x g for 1 min ...
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bioRxiv - Microbiology 2023Quote: ... the different antibodies were previously incubated at 4°C with protein G-coated magnetic beads (DiaMag, Diagenode), protease inhibitor cocktail and 0.1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were sonicated for 4 min (20 seconds on, 40 seconds off) using a Bioruptor Pico (Diagenode). After sonication samples were cleared with 1% triton X-100 and pelleted (16000 x g for 5 min at 4 °C) ...
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bioRxiv - Genomics 2022Quote: ... the nuclei were briefly sonicated for 5 seconds at 4°C in a Bioruptor Pico sonicator (Diagenode). Permeabilized nuclei were incubated for 10 minutes at 37°C in a thermoshaker ...
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bioRxiv - Neuroscience 2020Quote: ... and sonicated 10 times 30s on/off in a Bioruptor sonicator (Diagenode). Lysates were diluted 10-fold and quantified by BCA assay against a BSA standard curve in diluted lysis buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Chromatin was immunoprecipitated with 10 ug of H3K27Ac antibody (Diagenode cat# C15410196), 10 ug of ASCL1 antibody (abcam ab74065 ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by sonication (10 minutes, every 30 seconds) (Bioruptor Pico Diagenode, Belgium). Next ...
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bioRxiv - Biochemistry 2024Quote: ... The ultrasonic pyrolysis cycle included 10-sec ultrasonic pyrolysis (Diagenode, Seraing, Belgium) and 30-sec interval ...
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bioRxiv - Genomics 2020Quote: Preparation of reagents: ChIP Wash buffers 1 to 4 (from iDeal ChIP-seq kit for histones, Diagenode C01010173) are aliquoted into quarter module reservoirs divided by length ...
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bioRxiv - Plant Biology 2024Quote: ... slides were incubated over night at 4°C with primary-antibody (H3K9me2: Diagenode: C15200154; H3K27me1: Diagenode: C15410045-50) diluted in 1% BSA ...
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bioRxiv - Developmental Biology 2024Quote: ... The sonication of samples was then performed at 4°C using a Bioruptor® Plus sonication device (Diagenode) at high-intensity settings ...
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bioRxiv - Molecular Biology 2024Quote: ... They were subsequently sonicated for 4 cycles (30 sec on, 30 sec off) using the Bioruptor Pico (Diagenode) and centrifuged for 15 min at 14,000 x g ...
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bioRxiv - Physiology 2024Quote: ... and 500 µl volume) in a water bath sonicator set at 4°C (Bioruptor 300. Diagenode, Denville, NJ). After centrifuging at 10,000g for 10 min at 4°C ...
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bioRxiv - Genomics 2020Quote: ... total on-time 10 min) with a Biorupter® Pico Sonicator Device (Diagenode). 5 - 33 μg of sonicated chromatin was incubated with 4 μg ChIP-validated CTCF antibody (Active Motif ...
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bioRxiv - Cancer Biology 2020Quote: ... After sonication (30”on/30” off, 10’ total time) in a Bioruptor (Diagenode) to disrupt aggregates ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples in lysis buffer were boiled for 10 minutes and sonicated (Bioruptor, Diagenode) for 15 minutes at room temperature ...
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bioRxiv - Genomics 2024Quote: ... incubated for 10 min on ice and sonicated with a Bioruptor Pico (Diagenode) for five cycles of 30s on and 30s off ...
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bioRxiv - Genomics 2024Quote: ... 10 μg of HMW DNA were sheared with either Megaruptor 3 (Diagenode; #B06010003) or a needle (FINEJECT ® 26Gx1” 0.45x25mm ...
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bioRxiv - Genetics 2021Quote: ... ONT libraries were prepared using the EXP-NBD103 and SQK-LSK109 kits according to the manufacturer’s instructions and using 4 µg of 40Kb sheared DNA (Megaruptor, Diagenode) as input ...
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bioRxiv - Immunology 2021Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 × g ...