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Citations for Diagenode :
51 - 100 of 2740 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Chromatin samples were sheared using a sonicator set at “high” with 30 s pulse on/off cycles (Diagenode Bioruptor). Pulse cycles were optimised (9-15 cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were sonicated (5 times 5 min, 30 sec on/off) with a Bioruptor (Diagenode) and pelleted (13000 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... for 5 min at 4°C and lysates were sonicated (1x 5 min, 30 sec on/off) with a Bioruptor (Diagenode) followed by digestion with 40U RNaseI (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by 10 sec sonication with a Bioruptor (Diagenode) at low energy and incubation with 10 U Benzonase (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA was sheared to ∼400-500bp using a Bioruptor Pico Sonicator (Diagenode, Denville, NJ, USA), and used a HS D1000 ScreenTape run on a Tapestation 2200 (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were sonicated (5 times 5 min, 30 sec on/off) with a Bioruptor (Diagenode) and pelleted (13000 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin was sheared into ∼50–300 bp fragments by sonication using Bioruptor (Diagenode).
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bioRxiv - Molecular Biology 2024Quote: ... H3K9ac (Diagenode) and HA (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... Ctcf (Diagenode, C15410210), or Hnf4a (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2024Quote: ... and H2AK119Ub (Diagenode, C15410195 ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 7.8) where tagmentation by assembled Tn5 (Diagenode, C01070010) complex was performed ...
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bioRxiv - Genomics 2024Quote: ... using a Microplex v3 kit (Diagenode).
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bioRxiv - Genomics 2024Quote: ... a µChIPmentation protocol (Diagenode) was used ...
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bioRxiv - Molecular Biology 2024Quote: ... for a total volume of 100 μL and sonicated (4 cycles of 20sec/30sec (on/off time)) using a Bioruptor (Pico II, Diagenode, BE). The successful generation of fragments with a mean fragment size of 400 nucleotides was confirmed using a Bioanalyzer as per manufacturer’s instructions (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... and sonicated (6 cycles, high power for 30 s on and 30 s off at 4 °C, Bioruptor NextGen (Diagenode)) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were sonicated (settings: 3 – 4 x 30 sec on /30 sec off, 4°C, power high; Bioruptor, Diagenode) and lysates were cleared by centrifugation at 16,000 x g at 4°C for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The DNA was sheared by sonication (4x 7 cycles, 30s on / 30s off, power high; Bioruptor, Diagenode) at 4°C and lysates cleared by centrifugation at 16,100 x g at 4°C for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref: 901503; anti-H3K4me3 Cell Signaling, ref. 9751S; anti-H3K36me3 Diagenode, ref ...
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bioRxiv - Neuroscience 2024Quote: ... and sonicated with 30 cycles of 15 s ON-OFF using a BioRuptor (Diagenode, Denville, NJ-USA). The EV lysate was reduced with 10 mmol/L dithiothreitol at 56 °C for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref ...
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bioRxiv - Molecular Biology 2024Quote: ... the insoluble pellet was resuspended in RIPA buffer (Cold Spring Harbor Protocol) and followed by 15min sonication by Diagenode bioruptor with medium power for 30s on and 30s off at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... H3K27me3 (Diagenode, C1541095, 1:2000), Total H3 (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... The nuclear pellets were lysed with lysis buffer (50 mM Tris-HCl, 10 mM EDTA, 1% SDS, pH 8) and then sonicated with Bioruptor (Diagenode, 15 cycles, 30s ON/30s OFF). The size of the DNA in the sheared chromatin fragments was checked before precipitation by a Tapestation system (Agilent 4200 ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin was extracted and sheared with the Bioruptor sonicator (Diagenode). For the intended downstream normalization of ChIP samples ...
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bioRxiv - Neuroscience 2024Quote: ... The lysates were then sonicated with 3 × 10 cycles (30 s on and off) (Bioruptor, Diagenode) to generate chromatin fragments of ∼500 bp in length ...
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bioRxiv - Molecular Biology 2024Quote: ... C15410206 (Diagenode). Input and pulldown DNA was analyzed in triplicates by qPCR with iTaq Universal SYBR Green supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... buffer with added protease and phosphatase inhibitors and sonicated on high for 30 seconds off 30 seconds on for 3 minutes (Diagenode Bioruptor). The protein concentration was determined using the Micro BCA TM protein assay kit (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... in a 1.5 mL TPX microtube (Diagenode) and incubated on ice for 15 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were transferred into 15-ml TPX tubes and sonicated using Bioruptor Plus (Diagenode) with the following parameters ...
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bioRxiv - Molecular Biology 2024Quote: ... ChIP (Chromatin Immunoprecipitation) was performed using the reagents and protocol provided in the universal plant ChIP-Seq kit (Cat. No. C01010152; Diagenode, Ougree, Belgium) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by 10 min sonication (Diagenode Bioruptor). The extracts were then precleared ...
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bioRxiv - Molecular Biology 2024Quote: ... The sheared chromatin was immunoprecipitated using the DiaMag protein A-coated magnetic beads (Diagenode) and 2 µg of anti-HvHsfA6a antibodies ...
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bioRxiv - Developmental Biology 2024Quote: ... Ribosome profiling sequencing libraries were prepared using the D-Plex Small RNA-seq kit (C05030001, Diagenode) with slight modifications ...
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bioRxiv - Molecular Biology 2024Quote: ... Crosslinked samples were washed in phosphate buffered saline (PBS), resuspended in ChIP lysis buffer (0.5% SDS, 10mM EDTA, 50mM Tris-HCl pH8) and sonicated (Bioruptor Plus, Diagenode) to release 100-1000 bp fragments ...
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bioRxiv - Microbiology 2024Quote: ... The Mock (15 ng in 20 µl TE buffer) was randomly fragmented by BioRuptor (Diagenode, Liege, Belgium) at default intensity for 15 sec on and 90 sec off ...
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bioRxiv - Microbiology 2024Quote: ... and the samples were heated for 5 min at 95°C prior to sonication with a Bioruptor UCD-200 (Diagenode, Seraing, Belgium) device for ten cycles of 1 min pulse and 30 sec pause at high power ...
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bioRxiv - Microbiology 2024Quote: ... Immunoprecipitation was done with 2 µg of chromatin and 4 µg of antibody against H3K9me3 (Diagenode, C15410193, lot A2217P). Sequencing libraries were prepared from 5 ng of immunoprecipitated DNA as previously described [19 ...
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bioRxiv - Cell Biology 2024Quote: ... using a Bioruptor sonicator (Diagenode, Belgium). 10x 30 second cycles of sonication were used on a high setting ...
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bioRxiv - Cancer Biology 2024Quote: ... or H3K27me3 (Diagenode C15410195) overnight at 4 °C with rotation ...
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bioRxiv - Bioengineering 2024Quote: ... The cell lysates were heated at 95[ for 5 minutes and sonicated for 15 min using a Bioruptor Plus (Diagenode). The supernatant was collected after centrifugation at 18,000 g for 30 min at 4[ ...
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bioRxiv - Plant Biology 2024Quote: ... Chromatin was sheared using a Bioruptor Plus (Diagenode) (20 cycles of 30 seconds on and 30 seconds off ...
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bioRxiv - Molecular Biology 2024Quote: ... coupled to either CTD Ser2P (C15200005; Diagenode) or Ser5P (C15200007 ...
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bioRxiv - Molecular Biology 2024Quote: ... or Ser5P (C15200007; Diagenode) antibodies ...
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bioRxiv - Genomics 2024Quote: ... DNA was then sheared with Megaruptor 3 (Diagenode) (45/200/30 ...
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bioRxiv - Molecular Biology 2024Quote: ... or anti-Ser5P (C15200007-50, Diagenode). Then beads were washed twice for 15 min with Low Salt and High Salt buffers like for histones but including 1x of PhosSTOP ...
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bioRxiv - Microbiology 2024Quote: ... The DNA was sheared into ∼200 bp fragments by ultra-sonication (Bioruptor Plus Diagenode). Fragmented DNA samples were prepared for Illumina sequencing using the NEBNext Ultra I DNA Library Prep Kit for Illumina according to manufacturer’s instructions with the following modifications ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then subjected to Bioruptor® treatment (Diagenode) for 10 minutes (30 sec on ...
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bioRxiv - Microbiology 2024Quote: ... were added to the lysate and incubated at 37°C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Molecular Biology 2024Quote: ... Bioruptor Pico (Diagenode).
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bioRxiv - Cell Biology 2024Quote: ... Chromatin was sonicated using a Bioruptor Pico (Diagenode, B01060010), 5 cycles of 30 seconds “on” and 30 seconds “off” on ice-cold water ...