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Citations for Stemcell Technologies :
351 - 400 of 763 citations for Phosphatase Inhibitor Cocktail 2 Tubes 100X since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Israel directly after surgery and were transported to our laboratory on ice in sterile tubes containing HypoThermosol® FRS medium (StemCell Technologies). Tissue was processed within 72 hours of surgery ...
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bioRxiv - Immunology 2020Quote: ... Remaining whole blood was diluted 1:1 with 1% RPMI (Table S4) and layered into a SEPMATE tube (STEMCELL Technologies, Cat#85450) pre-loaded with lymphoprep (Alere Technologies ...
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bioRxiv - Immunology 2023Quote: PBMCs from peripheral blood from study participants or buffy coats from blood bank donors were isolated using SepMateTM Tubes and LymphoprepTM separation media (both StemCell Technologies, USA) utilizing the density gradient technique per manufacturers’ recommendation ...
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bioRxiv - Immunology 2023Quote: ... PBMC were isolated from blood collected in ethylenediaminetetraacetic acid-anticoagulated (EDTA) tubes by lymphocyte separation medium density gradients (Stemcell technologies, cat# 07801) and resuspended in PRMI 1640 medium supplemented with 10% FCS ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were diluted 1:1 with phosphate-buffered saline (PBS) and MNC were enriched using SepMate-50 tubes (Stem Cell Technologies, 85460) and Pancoll separation solution (PAN Biotech ...
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bioRxiv - Cancer Biology 2024Quote: ... The tissue was finely minced and placed in 15 ml conical tubes with a dissociation solution composed of Collagenase/Hyaluronidase (Stem Cell Technologies) enzymes ...
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bioRxiv - Neuroscience 2019Quote: ... with medium changes every 2-3 days and passages 1-2 times per week using ReLeSR (Stem Cell Technologies). Heteroplasmy levels were regularly measured to insure they were retained across passage numbers ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10 µM of Rock inhibitor Y-27632 and 1x CloneR (Stemcell Technologies). Repair of CALRins5 and CALRdel52 mutation was verified by allele-specific or flanking PCR ...
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bioRxiv - Cell Biology 2022Quote: ... and plated in media containing 10 μM Rho-kinase inhibitor Y-27632 (StemCell Technologies). Embryonic body (EB ...
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bioRxiv - Neuroscience 2021Quote: ... in Essential 8 medium with ROCK inhibitor Y-27632 (10 μM, #72308, STEMCELL Technologies). After 24 h ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were exposed to the Wnt signaling inhibitor IWP-4 (STEMCELL Technologies, cat. 72552) for 2 days from days (D ...
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bioRxiv - Developmental Biology 2019Quote: ... Medium was further supplemented with 1μg/ml ROCK inhibitor Y-27632 (Stem Cell Technologies) and replaced every 48h ...
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bioRxiv - Microbiology 2020Quote: ... The inhibitors were removed and replaced with fresh PneumaCult™-ALI Medium (STEMCELL Technologies) prior to SARS-CoV-2 infection at an MOI of 0.1 ...
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bioRxiv - Genomics 2021Quote: ... with Rock inhibitor Y-27632 (10µM final concentration; Stem Cell Technologies; Catalog Number: 72304) on Costar non-treated 6 well polystyrene plates (Catalog Number ...
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bioRxiv - Neuroscience 2021Quote: ... and media with 10 μM Rho-associated kinase (ROCK) inhibitor (Y-27632, STEMCELL Technologies) was used for any single-cell dissociation ...
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bioRxiv - Bioengineering 2021Quote: ... coiled-coil containing protein kinase (ROCK) inhibitor (5 μM, StemCell Technologies, Vancouver, BC, Canada). Until 95% confluency was reached ...
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bioRxiv - Developmental Biology 2021Quote: ... supplemented for 3 days with 10 μM Rock inhibitor Y-27632 (StemCell Technologies, 78003).
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bioRxiv - Developmental Biology 2023Quote: ... supplemented with 10 μM Rho-kinase inhibitor (RockI; Y-27632 STEMCELL Technologies, cat. 72304). iPSCs were seeded at a density of 6×104 cells/cm2 and expanded for 3 days to achieve 80% confluency prior to differentiation ...
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bioRxiv - Microbiology 2023Quote: ... in StemFlex media supplemented with 10LJμM ROCK inhibitor Y-27632 (STEMCELL Technologies, Vancouver, Canada). From days 1-5 ...
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bioRxiv - Developmental Biology 2023Quote: ... was supplemented with 10 µM ROCK-Inhibitor (Catalog no. Y-27632, Stem Cell Technologies) during the first 4-days ...
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bioRxiv - Cancer Biology 2020Quote: GFP positive neural tube regions were dissected after a DispaseI-DMEM/F-12 treatment (Stem cell technologies 1U/ml #07923; 37°C, 30min). Single cell suspensions were obtained by 3 minutes incubation in Trypsin-EDTA 0.05% (Life technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... The epidermis was separated from the dermis by placing the strips in tubes containing 10 ml of 5U/ml Dispase II (Stem Cell Technologies, USA) and incubated at 4°C overnight and at 37°C for 2-3 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... from participants with HIV-1 on ART were isolated from whole blood by density gradient centrifugation using Lymphoprep™ and SepMate™ tubes (StemCell Technologies), and cryopreserved [41] ...
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bioRxiv - Cell Biology 2023Quote: ... buffy coat-PBS-citrate solution was added in portions of 25 ml to tubes filled with 10 ml LymphoprepTM (07851, StemCell technologies, Köln, Germany). Tubes were centrifuged (20 min at RT ...
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bioRxiv - Immunology 2023Quote: ... BMDC were resuspended in PBS in a 12 × 75 mm polypropylene tube and placed in a EasySep™ magnet (Stemcell Technologies, Vancouver, CAN) for 5 minutes ...
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bioRxiv - Bioengineering 2023Quote: Mononuclear cells from healthy human whole blood and CLL patients were isolated by density centrifugation using SepMate-50 tubes (STEMCELL Technologies, Vancouver, Canada). In short ...
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bioRxiv - Cancer Biology 2023Quote: ... and 2 H3.1-K27M (GSC3, GSC4) cellular models were grown in NeurocultTM medium supplemented with heparin (2 μg/mL, Stemcell Technologies), EGF/FGF (20ng/mL ...
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bioRxiv - Immunology 2021Quote: ... NK cell isolation was done by using the negative selection kit “RosetteSep™ Human NK Cell Enrichment Cocktail” (STEMCELL Technologies) according to the company’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: T cells were purified from normal donors by negative selection using the RosetteSep Human CD4+ T Cell Enrichment Cocktails according to the manufacturer’s protocols (StemCell Technologies). Primary cells were activated and expanded using Human T-activator CD3/CD28 Dynabeads (Cat # 11161D ...
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bioRxiv - Immunology 2019Quote: ... LV-CAR T cells were expanded by stimulation with an antibody cocktail (anti-CD3/CD28; STEMCELL Technologies; cat. no. 10971) combined with IL-2 (50 U/ml ...
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bioRxiv - Bioengineering 2021Quote: ... Primary human T cells were isolated using negative selection per the manufacturer’s instructions (RosetteSep Human T Cell Enrichment Cocktail, STEMCELL Technologies). T cells were counted using a Countess II FL Automated Cell Counter (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Total CD4+ T cells were separated by negative selection using RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies). Primary CD4+ T cells were plated at a concentration of 1,5×10 6 cells/mL left overnight for recovery and infected ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with a cocktail of lineage specific antibodies (CD5, CD11b, CD19, B220, Gr-1 and TER119, Stemcell Technologies) for negative selection of Lin-cells using an EasyStep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... DNTs were enriched by depleting CD4+ and CD8+ cells using RosetteSep™ human CD4- and CD8-depletion cocktails (Stemcell Technologies). The CD4 and CD8 depleted cells were cultured in 24-well plates pre-coated with 5◻μg/ml anti-CD3 antibody (OKT3 ...
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bioRxiv - Microbiology 2022Quote: ... Monocytes were isolated from whole blood by negative selection using RosetteSep human monocyte enrichment cocktail following the manufacturer’s protocol (StemCell Technologies). Using density gradient medium (Lymphoprep ...
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bioRxiv - Synthetic Biology 2020Quote: ... CD4+ and CD8+ T cells were isolated using the RosetteSep Human T cell enrichment cocktail (STEMCELL Technologies, 15022 and 15023), and PBMCs were isolated using Lymphoprep (STEMCELL Technologies ...
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bioRxiv - Biophysics 2020Quote: ... The cells were resuspended in 1 mL of medium contained 1x Stem Span Cytokine Cocktail CC100 (Stem Cell Technologies, Canada) in 24-well plate ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from healthy adults using the RosetteSep human CD4+ T cell enrichment cocktail (STEMCELL Technologies, 15062) followed by a density gradient isolation (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... CB MNC were enriched for CD34+ cells using the RosetteSepTM Human CB CD34 Pre-Enrichment Cocktail (Stem Cell Technologies, UK) as per manufacturer’s instructions ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from elutriated lymphocytes by negative selection using RosetteSep CD4+ T cell enrichment cocktail (StemCell Technologies) followed by Ficoll/Hypaque (Amersham Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were negatively isolated using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (Stem Cell Technologies # 15061) or the EasySepTM Human Naïve CD4+ T cell Isolation Kit (Stem Cell Technologies #17953 ...
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bioRxiv - Bioengineering 2023Quote: Primary human CD3+ T cells from 3 unique donors were isolated by negative selection (T Cell Enrichment Cocktail, Stemcell Technologies) from peripheral blood and were grown in RPMI + 10% FBS + 1% PS supplemented with 50 U/mL of IL2 ...
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bioRxiv - Cell Biology 2023Quote: ... Primary NK cells were isolated from peripheral blood by negative selection using RosetteSep human NK enrichment cocktail (Stemcell Technologies, 15065). Blood was layered onto a Ficoll-Paque (Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... 2 μg/mL Heparin (StemCell Technologies, Cat 07980), 1× antibiotic-antimicotic (Life Technologies Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... heparin (2 μg/ml; Stem Cell Tech, #07980), and Antibiotic-Antimycotic solution (0.1% ...
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bioRxiv - Genetics 2022Quote: ... 2 μM doxycycline and CloneR supplement (StemCell Technologies) to enhance the viability of single cells ...
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bioRxiv - Neuroscience 2020Quote: ... and heparin (2 μg/mL, StemCell Technologies, #07980).
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bioRxiv - Cancer Biology 2020Quote: ... 2 μg/mL Heparin (Stem Cell Technologies #07980) and L-glutamine (2mM ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 2% NeuroCult SM1 supplement (STEMCELL Technologies) and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... containing 2% NeuroCult SM1 supplement (StemCell Technologies, 05711), 1% L-glutamine (Corning ...