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Citations for Stemcell Technologies :
501 - 550 of 763 citations for Phosphatase Inhibitor Cocktail 2 Tubes 100X since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Cells were plated in mTeSR1 + 1% P/S and 10 µM Rock Inhibitor (Y-27632, StemCell Technologies, #72308). A full detailed protocol for cell line maintenance can be found at www.allencell.org/sops SOP ...
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bioRxiv - Developmental Biology 2022Quote: ... The cell pellet was resuspended in 1ml of FC medium with 7.5nM ROCK inhibitor (Y27632, STEMCELL Technologies 72304). After adding the cell mixture dropwise to the AggreWell ...
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bioRxiv - Developmental Biology 2023Quote: ... and kept on Rho-associated protein kinase (ROCK) inhibitor Y-27632 (final concentration 5 μM, StemCell Technologies, #72302) for one day ...
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bioRxiv - Cancer Biology 2020Quote: ... Pellet is resuspended in 2-5ml accutase™ (StemCell Technologies, 07920), depending on the sample amount ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were collected with 2 mL mTeSRplus (Stem Cell Technologies, 05825), span down and resuspended in 1 mL mTeSRplus ...
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bioRxiv - Cancer Biology 2020Quote: ... Pellet is resuspended in 2-5ml accutase™ (StemCell Technologies, 07920), depending on the sample amount ...
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bioRxiv - Bioengineering 2021Quote: ... additionally supplemented with cytokines (Human Recombinant IL-2; Stemcell Technologies - #78036). To stimulate the cells ...
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bioRxiv - Cancer Biology 2022Quote: ... PHA was washed and IL-2 (50 U/ml, StemCell Technology) was added to the medium and replaced every 2 days for a time period of 5 days.
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bioRxiv - Neuroscience 2023Quote: ... Each plate was then split 1:2 using ReLeSR (StemCell Technologies), with half of the cells used for DNA extraction for Sanger sequencing ...
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bioRxiv - Physiology 2019Quote: Monocytes were isolated by the University of Pennsylvania Human Immunology Core from healthy apheresis donors with institutional review board approval by RosetteSep™ human monocyte enrichment cocktail (Stem Cell Technologies, Vancouver, Canada), and MΦs were differentiated by 12-days adherence culture in RPMI 1640 media containing 10% human serum and 1x cell culture pen/strep antibiotic mix (Gibco) ...
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bioRxiv - Immunology 2021Quote: Untouched total T or isolated CD4+ or CD8+ T cells were purified from buffy coats or whole blood with RosetteSep Human T or CD4+ or CD8+ T cell enrichment cocktails (STEMCELL Technologies, Cat#: 15061, 15062, 15063). Memory CD4+ or CD8+ T cells were either isolated from PBMCs by EasySep Human Memory CD4+ or CD8+ T cell enrichment kit (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were cultured in mTeSR plus medium supplemented with 10 µM Rho-associated protein kinase (ROCK) inhibitor (Stemcell Technologies). After overnight incubation ...
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bioRxiv - Cell Biology 2022Quote: ... they were cultured during 24 hours in the presence of 10 μM of Rock inhibitor Y-27632 (StemCell Technologies).
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bioRxiv - Neuroscience 2022Quote: ... The iPSCs were thawed and incubated in mTeSR1 media supplemented with 0.5 µM Rock Inhibitors (StemCell Technologies, cat.no. 72304) for 24 hours at 37°C ...
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bioRxiv - Genomics 2022Quote: ... and quenched with DMEM/F-12 GlutaMAX supplemented with 10 µM of ROCK inhibitor (Y-27632, StemCell Technologies, #72304) and 0.033 mg/ml of DNase (DNase (D2) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were cultured in the presence of TGFβ and BMP inhibitors (LDN193189, #72142, Stem Cell Technologies; SB431542, #1614, Tocris) to trigger cortical precursors for cortical neuron differentiation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and cultured overnight in mTeSR Plus medium supplemented with 10 µM Y-27362 ROCK inhibitor (Stem Cell Technologies, Canada). Differentiation was induced on day 0 by first washing cells with PBS when the monolayer reached approximately 80% confluence ...
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bioRxiv - Developmental Biology 2024Quote: ... and were plated in a 6-well plate containing 10 µM Rock inhibitor Y-27632 (Stem Cell Technologies, 72302). Twenty-four hours post transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 million cells were passaged per each well using Accutase (StemCell Technologies) into the STEMdiff SMADi neural induction medium (StemCell Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10 ng μL-1 recombinant human IL-2 (StemCell Technologies) and activated with the ImmunoCult Human CD3/CD28 T Cell Activator (StemCell Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 ng/ml basic-FGF and 2 μg/ml Heparin (StemCell Technologies). L0125 and L0512 GBM tumorspheres were kindly provided by Dr ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Cells were passaged every 2-3 days using accutase (Stemcell Technologies, #07920). Cells were then collected by centrifugation at 1200 rpm for 3 minutes and counted before seeding ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 ng/ml basic-FGF and 2 μg/ml Heparin (StemCell Technologies). S24 Tdtomato/GFP cells were kindly gifted by Dr ...
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bioRxiv - Neuroscience 2022Quote: ... Heparin solution 2 μg/ml (cat# 07980, Stem Cell Technologies, Vancouver, Canada), EGF 20 ng/ml (Cat#315-09 ...
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bioRxiv - Molecular Biology 2023Quote: HUDEP-2 cells were grown in StemSpan SFEM (STEMCELL Technologies, Vancouver, Canada) supplemented with 50ng/mL stem cell factor ...
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bioRxiv - Cell Biology 2023Quote: ... 5% CO2 for 2 weeks in supplemented mTeSR medium (STEMCELL Technologies #85850). For KO of C3orf58 or SLC39A9 in iPSC ...
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bioRxiv - Cancer Biology 2023Quote: ... and fed IL-2 (50 U/mL; Stem Cell Technologies, Vancouver, Canada) every 48 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... HUDEP 2 cells were cultured in Stemspan Media (SFEM II - STEMCELL Technologies) with 50ng/ml SCF (ImmunoTools) ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µM (for iPSC) or 5 µM (for ESC) XAV939 (Stemcell Technologies), and 10 µM Y27632 ...
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bioRxiv - Bioengineering 2023Quote: ... with the addition of Human Recombinant Interleukin 2 (STEMCELL Technologies, Cat# 78036.3). To prepare 3 mg/ml collagen-I gel ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were passaged 1-2 times per week with ReLeSR (STEMCELL Technologies) when they reached 80-90% confluence ...
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bioRxiv - Genomics 2019Quote: ... iPSCs were dissociated with 1:3 Accutase and plated onto 24-well plates pre-coated with 0.5% GelTrex in mTeSR1 supplemented with 10uM Y-27632 ROCK Inhibitor (STEMCELL Technologies). 24 hours later another layer of GelTrex at 1.5% was added in mTeSR1 media ...
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bioRxiv - Developmental Biology 2021Quote: ... colonies were disaggregated into single cells using Accutase and plated in the presence of Rock Inhibitor (StemCell Technologies Y-27632) on matrigel-coated plates ...
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bioRxiv - Developmental Biology 2022Quote: ... cultured in either H9 hESC or DEF-CS media for 48 hours with 10µM ROCK inhibitor (Y-27632, STEMCELL Technologies) for first 24 hours and maintained in 20% O2/5% CO2/37°C ...
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bioRxiv - Genomics 2019Quote: ... and quenched with complete STEMflex media containing 10-µM RHO/ROCK pathway inhibitor (Ri) Y-276932 (STEMCELL Technologies Cat#72304). Cells were subsequently spun down at 300 rcf for 5 minutes and resuspended in media supplemented with Ri ...
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bioRxiv - Developmental Biology 2023Quote: ... 1x penicillin-streptomycin and 10ng/ml FGF2 (Department of Biochemistry, University of Cambridge) and 10µM ROCK inhibitor Y-27632 (72304, STEMCELL Technologies). For RSeT cells ...
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bioRxiv - Bioengineering 2023Quote: ... Embryoid bodies formed via hanging drop approach with each droplet containing 3-4×104 human single PSCs in 20 μl were cultured hanging on TESR-E8 supplemented with Polyvinyl Alcohol (PVA) at 4mg/ml (SigmaAldrich) and rock inhibitor (Y-27632) at 10μM (StemCell Technologies) at the lid of Petri dishes ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... counted and added at 1.2 million cells per microwell in AggreWell media containing 10 μM ROCK inhibitor (StemCell technologies 72302). Media was carefully changed the following day to remove ROCK inhibition ...
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bioRxiv - Genomics 2023Quote: ... resuspending cell pellet in mTeSR Plus Basal Medium supplemented with 0.1% dihydrochloride ROCK Inhibitor (Stemcell technologies; Cat. No. Y-27632), counting and plating onto Geltrex-coated plates at the desired number.
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bioRxiv - Neuroscience 2023Quote: ... was pipetted into the cuvette and incubated for 10 minutes at 37°C and then transferred to a fresh well of a Matrigel coated 6-well plate with 2ml of mTeSR1 supplemented with ROCK-inhibitor (10μM, StemCell Technologies). Single GFP-positive cells were sorted in a 96-well plate with mTeSR plus CloneR (StemCell Technologies) ...
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bioRxiv - Cell Biology 2019Quote: ... In either situation cells were resuspended in APEL 2 medium (Stem Cell Technologies) containing 10µM ROCKi ...
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bioRxiv - Bioengineering 2021Quote: ... and 100ng/mL human recombinant Fibroblast Growth Factor 2 (FGF2, STEMCELL Technologies, 78003.1) in RPMI 1640 for 2 days ...
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bioRxiv - Bioengineering 2021Quote: ... and 100ng/mL human recombinant Fibroblast Growth Factor 2 (FGF2, STEMCELL Technologies, 78003.1) for 2 days ...
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bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human recombinant Fibroblast Growth Factor 2 (FGF2, STEMCELL Technologies, 78003.1), 20 ng/mL animal component-free human recombinant Glial Cell Derived Neurotrophic Factor (GDNF ...
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bioRxiv - Microbiology 2021Quote: ... About 2×105 cells were washed twice in Robosep buffer (#20104, Stemcell Technologies) and fixed in 300 μl CellFix buffer 1X (#340181 ...
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bioRxiv - Neuroscience 2021Quote: ... medium was replaced with 2 mL/well iHSC Medium B (StemCell Technologies #05310). An additional 1 mL/well of iHSC Medium B was added every other day ...
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bioRxiv - Cancer Biology 2020Quote: ... thawed organoids were rinsed with 2% fetal bovine serum (FBS, from STEMCELL Technologies) in Hank’s Balanced Salt Solution (HF) ...
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bioRxiv - Genetics 2020Quote: ... Expansion phase medium for HUDEP-2 cells consists of SFEM (Stemcell Technologies #09650) base medium supplemented with 50 ng/ml recombinant human SCF (R&D systems #255-SC) ...
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bioRxiv - Genomics 2020Quote: HUDEP-2 cell lines were cultured in StemSpan™ SFEM II (STEMCELL Technologies) along with 50 ng/ml SCF (ImmunoTools) ...
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bioRxiv - Genomics 2021Quote: ... 2×107 T cells were aliquoted into CryoStor cryopreservation media (Stem Cell technologies) for long-term storage.