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Citations for Stemcell Technologies :
601 - 650 of 763 citations for Phosphatase Inhibitor Cocktail 2 Tubes 100X since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were plated into 96 well flat bottom plates in growth medium supplemented with 10 µM of ROCK inhibitor (Stem Cell Technologies). Cells were plated at low density (2,500 cells/well ...
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bioRxiv - Neuroscience 2023Quote: ... in 150 μl neural induction media and kept in suspension under rotation (95 rpm) in the presence of 50 μM ROCK inhibitor (Stem cell Technologies) and kept in suspension under rotation (85 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... and 2.8×105 cells/cm2 were plated on Geltrex-coated plates in mTesR+ supplemented with ROCK inhibitor Y-27632 (10uM, StemCell Technologies, 72302). Neuronal induction was started 24h later (day 0 ...
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bioRxiv - Genomics 2023Quote: ... single hESC cells were plated at a density of approximately 5×105 per each well of a 6 well plate in the presence of ROCK inhibitor and exposed to commercially available mesoderm induction media (Stemcell Technologies 05221).
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bioRxiv - Genomics 2023Quote: ... in the presence of ROCK inhibitor and exposed to commercially available mesoderm induction media as instructed by the supplier (Stemcell Technologies 05221). After four days of treatment cells were dissociated into single cells with Accutase (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... The cell culture medium was supplemented with 1:200 Rho-associated protein kinase inhibitor (ROCKi) Y-27632 (final concentration 5 µM, STEMCELL Technologies, 72302) on the first day after passage ...
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bioRxiv - Genomics 2023Quote: ... at 70-80% confluency using EZ-LiFT Stem Cell Passage Reagent and maintained for 24 hours in mTESR1 medium plus 10µM rock inhibitor Y-27632 (STEMCELL Technologies, cat. 72308). Cells were grown in mTESR1 and the media was changed every 24 hours until cells were 75-85% confluent ...
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bioRxiv - Neuroscience 2024Quote: ... the cell pellet was mixed gently with STEMdiff™ Neural Induction Medium (NIM)+1µl/ml ROCK inhibitor Y-27632 (#72302; Stem Cell Technology) and plated at 3×106 cell density per well into Nunclon Sphera 96 U bottom plates (#174929 ...
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bioRxiv - Microbiology 2020Quote: ... hNPCs were grown on Matrigel-coated culture plates in Neural Progenitor Medium 2 (StemCell Technologies) up to 95-100% confluency ...
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bioRxiv - Cancer Biology 2021Quote: ... Hu Recom IL-2 (CHO Expr.) were purchased from Stem Cell Technologies (Vancouver, BC, Canada). The ATPLite Luminescence Assay System and 96-well white ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×103 cells were resuspended in 1 ml of complete MammoCultTM Basal Medium (Stemcell Tech) and seeded in ultralow attachment plates ...
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bioRxiv - Bioengineering 2023Quote: ... media was replaced with 2 mL Hematopoietic Media A (STEMdiff Hematopoietic Basal Media (StemCell Technologies) with STEMdiff Hematopoietic Supplement A (200x ...
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bioRxiv - Neuroscience 2023Quote: ... that was coated with 2 ml of anti-adherence rinsing solution (Stem cell technologies: 07010) for 5 mins before plating the cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Common Rule (Category 4) or 2) de-identified donor leukapheresis products obtained from StemCell Technologies. CD3+ T-cell populations were isolated using RosetteSep Human T Cell Enrichment Cocktails (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Common Rule (Category 4) or 2) de-identified donor leukapheresis products obtained from StemCell Technologies. CD3+ T-cell populations were isolated using EasySep Human T Cell Isolation Kit (StemCell Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... Differentiation was initiated by changing to RPMI1640 medium containing B-27 (minus insulin) and the glycogen synthase kinase-3 (GSK3) inhibitor CHIR99021 (STEMCELL Technologies, cat. 72052) for 24 h ...
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bioRxiv - Cell Biology 2021Quote: ... A total number of 3.75 × 105 ThF iPSCs were plated in a T25 flask in the mTeSR1 medium with ROCK Inhibitor Y-27632 (10 µM, Stem cell Technologies, #72304). After 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... cells were plated on Matrigel (In Vitro Technologies, cat. # FAL354277) coated plates in StemFlex medium supplemented with 10 μM ROCK inhibitor (StemCell Technologies, cat. #. 72304). Three days after plating ...
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bioRxiv - Cancer Biology 2022Quote: H1 human ESCs were dissociated using accutase and plated as single cells in mTeSR™1 medium supplemented with ROCK inhibitor Y-27632 (10 μM, StemCell Technologies, Inc.) on matrigel to a final density of 20,000-30,000 cells per cm2 ...
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bioRxiv - Developmental Biology 2021Quote: Cells were grown on TeSR1 or ectoderm medium and treated with the Activin/NODAL/TGF-β pathway inhibitor 2mM A83-0159 (StemCell Technologies #72022) for 72h ...
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bioRxiv - Developmental Biology 2022Quote: ... NPC-iPS cells were treated with 10 µM Rho kinase inhibitor Y-27632 overnight before dissociated to single cells with Accutase (Stem Cell Technology 07922). About 10 million cells were electroporated with 10 μg of AAVS1 zinc finger nuclease-encoding plasmid and 30 μg of AAVS1-tdTomato with one electroporation of 250 V ...
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bioRxiv - Cell Biology 2021Quote: ... to disperse into single cells and replated in mTeSR1 supplemented with 1% P/S containing 10 µM Rock Inhibitor (Y-27632, Stem Cell Technologies #72308). A full protocol is available at the Allen Cell Explorer (www.allencell.org/methods-for-cells-in-the-lab SOP ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1 × 106 cells were seeded per well of an Aggrewell™800 plate in APEL2 medium supplemented with 10 μM Y-27632 ROCK inhibitor (#72307, StemCell Technologies Inc.) followed by 72h incubation with daily media change ...
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bioRxiv - Cell Biology 2023Quote: ... 3.3 x 105 cells and 2.6 x 105 cells per ml were seeded in 3 ml E8 medium supplemented with 10 µM Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (RI, STEMCELL Technologies, Vancouver, Canada), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Beta-Mercaptoethanol Thermo Fisher Scientific 21985023) with double the concentration of dual-SMAD inhibitors (8uM Dorsomorphin Sigma-Aldrich P5499, 8uM of A-83 STEMCELL Technologies 72022), initiating neural induction and termed Day 0 ...
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bioRxiv - Genetics 2023Quote: ... The remaining positive cells were bulk sorted in a single well of a Vitronectin coated 6-well plate containing Essential 8 medium and ROCK inhibitor (STEMCELL Technologies, Cat#72304) for the purpose of measuring editing efficiency.
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bioRxiv - Developmental Biology 2024Quote: ... Medium was changed every second day and serial dilutions were performed in mTeSR+ medium containing Rock-inhibitor Y-27632 and CloneR (1:1000, Stem Cell Technologies #05889). Rock-inhibitor was removed after two days and the medium containing CloneR was changed every second day until colonies were ready to be picked ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells are resuspended in N2B27 medium supplemented with ROCK inhibitor and seeded at a density of approximately 75 cells per microwell in 400 µm AggreWell plates (Stem Cell Technology, cat# 34425). Once cell structures appear solid ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 2% FBS and 1.5ug/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies). Cells were stained for 20-30 minutes on ice with 1:200 anti-CD11b-BV711 (Clone M1/70 ...
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bioRxiv - Genomics 2020Quote: ... Tissues were minced into ~1-2 mm pieces and digested with 1× Collagenase/Hyaluronidase (STEMCELL Technologies) and 100 μg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... with the addition of the following supplements 2% (v/v) NeuroCultTM SM1 Neuronal Supplement (StemCell Technologies), 1% (v/v ...
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bioRxiv - Genetics 2022Quote: ... neuronal differentiation media was switched after 2 weeks to BrainPhys™ Neuronal Medium (Stem Cell Technologies) supplemented with the same factors to promote optimal neuronal activity and maturation.
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bioRxiv - Developmental Biology 2022Quote: ... Half well equivalent was seeded into 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Neuroscience 2022Quote: ... were maintained in serum-free STEMdiff™ Neural Progenitor Medium 2 (Stem Cell Tech cat#08560) on Corning® Matrigel® hESC-qualified Matrix (Corning cat#354277 ...
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bioRxiv - Genomics 2021Quote: Tissues were minced into ~1-2 mm pieces and digested with 1× Collagenase/Hyaluronidase (STEMCELL Technologies) and 100 μg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... C3H10T1/2 cells and MSCs were treated with Mesencult ™ Osteogenic Stimulatory Kit (STEMCELL TECHNOLOGIES, #05504).
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bioRxiv - Neuroscience 2022Quote: ... on day 2 media was changed to Neurobasal A and DMEM/F12 1:1 (Stemcell technologies) supplemented with N2 0.5% ...
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bioRxiv - Developmental Biology 2023Quote: ... with 6 μM CHIR99021 in 1.2 mL STEMdiff™ APEL™2 medium (STEMCELL Technologies, # 05270) at the bottom of the chamber for 1-hour ...
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bioRxiv - Cell Biology 2023Quote: ... 5 ng/ml FGF-2 (RnD Systems) and 1 μM CHIR99021 (Stemgent or Stem Cell Technologies). Mesoderm induction required daily medium exchanges for 3 days ...
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bioRxiv - Genetics 2022Quote: ... HUDEP-2 cells were expanded in a basal medium composed of StemSpan SFEM (Stem Cell Technologies) supplemented with 10−6 M dexamethasone (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... The following day the medium was replaced with STEMdiff™ APEL™2 (StemCell Technologies, #05270), containing 8 μM CHIR-99021 (Tocris ...
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bioRxiv - Cell Biology 2023Quote: ... medium was changed to 2 mL of complete STEMdiffTM Astrocyte Differentiation Medium (ADM) (Stemcell Technologies, #08540). On day7 and day 14 ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1 µM CHIR99021, a GSK3β inhibitor that activates Wnt signaling and limits apoptosis (Delepine et al., 2021; Qian et al., 2016) (Stem Cell Technologies, cat. no. 72054) and cultured 4 more days ...
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bioRxiv - Developmental Biology 2021Quote: Unsorted cells at day 6 (4+2) of differentiation were cultured in MethoCult 04434 (Stem Cell Technologies) in 35 mm dishes ...
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bioRxiv - Bioengineering 2022Quote: ... Both UC3-4 and UCS-2 hiPSCs were frozen in mFreSR medium (Stem Cell Technologies, Vancouver, Canada) and stored under cryogenic conditions in a vapor phase liquid nitrogen dewar ...
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bioRxiv - Cancer Biology 2021Quote: ... For the first 2 days cells were also exposed to 3 μM CHIR-99021 (Stem Cell Technologies). From day 4 for 5 days ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides are then stained for 1-2 days in Carmine Alum (Stem Cell Technologies; Catalog No. 07070) at RT ...
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bioRxiv - Cell Biology 2019Quote: ... incubated for 2 min in NH4Cl red blood cell lysis solution (StemCell Technologies, cat. # 07800, Vancouver, Canada), and seeded into culture plates in αMEM supplemented with 15% FBS and antibiotics ...
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bioRxiv - Bioengineering 2023Quote: ... the media was fully replaced with 2 mL Hematopoietic Media B (STEMdiff Hematopoietic Basal Media (StemCell Technologies) + STEMdiff Hematopoietic Supplement B (200x ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were expanded by culturing in RPMI 1640 with B27 with 2 μM of CHIR99021 (Stemcell Technologies). Media was replaced daily for 6-7 days or until cells reached confluency ...