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Citations for Stemcell Technologies :
251 - 300 of 763 citations for Phosphatase Inhibitor Cocktail 2 Tubes 100X since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Mononuclear cell subsets were obtained by isolation of PBMCs by gradient centrifugation in SepMate tubes (STEMCELL Technologies; cat. no. 85460), with Ficoll-Paque PLUS (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... The rest of the whole blood layer was diluted with an equal volume of PBS and then overlaid on 4.5 ml of Ficoll in a 15 ml SepMateTM tube (STEMCELL Technologies) and spun at 1200 rpm for 10 min at 20 °C to separate peripheral blood mononuclear cells (PBMCs) ...
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bioRxiv - Genomics 2022Quote: ... PBMC isolation was accomplished using SepMate tubes according to the manufacturer’s instructions with a few modifications (Stemcell Technologies, Cambridge, MA). Each 9 mL of blood was diluted in 15 mL of room temperature PBS and gently mixed for 15 min on a nutator ...
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bioRxiv - Immunology 2023Quote: ... uncoagulated whole blood from male and female donors (age range: 21-66) was spun at 1,200 × g in 50-mL SepMate tubes (STEMCELL Technologies) containing Ficoll-Paque PLUS (Cytiva ...
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bioRxiv - Immunology 2023Quote: ... Diluted blood was overlaid carefully on SepMate-15 tubes each containing up to 4 mL Lymphoprep (STEMCELL Technologies, catalog #07851). The layered blood was centrifuged at 1,200 × g for 10 minutes at RT ...
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bioRxiv - Immunology 2024Quote: ... Freshly harvested organ tissue from male and female bats was washed in PBS and cut into 3-5 mm small pieces before placing up to four individual pieces into a 1 ml cryostorage tube containing 0.5ml-1ml of Cryostore10 freezing medium (Stemcell Technologies). Vials were immediately transferred to a cryostorage container (Mr ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μg/ml Heparin (STEMCELL Technologies), 20 ng/ml bFGF (Millipore) ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 μg/ml heparin (StemCell Technologies), epidermal growth factor (EGF ...
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bioRxiv - Neuroscience 2020Quote: ... 2% NeuroCult SM1 supplement (STEMCELL Technologies), 2 mM GlutaMax.
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bioRxiv - Developmental Biology 2020Quote: ... 10ng/mL FGF-2 (StemCell Technology), and 5nM Rock inhibitor ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 µM SB431542 (StemCell Technologies). Cells were maintained in NbM for 6 days after which they were passaged with 1 U/mL Dispase (StemCell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... 2% SM1 (Stemcell Technologies, Vancouver, Canada), and 5 μM cytosine β-d-arabinofuranoside (AraC ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 μg/ml heparin (Stemcell Technologies), 50 units/ml penicillin ...
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bioRxiv - Neuroscience 2023Quote: ... B27 (2%v/v, Stemcell technologies), N2 (0.5%v/v ...
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bioRxiv - Neuroscience 2024Quote: ... 2% NeuroCult SM1 (05711, Stemcell technologies), 1% N2 Supplement (Thermo Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... and 10µM Y-27332 ROCK inhibitor (Stem Cell Technologies, removed after 24h). At cellular confluence ...
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bioRxiv - Developmental Biology 2022Quote: ... supplemented with 10 μM ROCK inhibitor (Y-27632, STEMCELL Technologies, Catalog# 12604013). Next day ...
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bioRxiv - Neuroscience 2020Quote: ... and LDN193189 (0.1μM, an inhibitor of BMP pathway) (72142, Stem Cell Technologies). The cells were subjected to neural induction for 12-15 days by changing the NIM every day ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with 5 nM Y-27632 dihydrochloride ROCK inhibitor (Stem Cell Technologies). The iPSCs were subsequently fed daily for 3 consecutive days with Neuronal Induction Medium (DMEM/F12 medium (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... the pan-PKC inhibitor Gö6983 was purchased from STEMCELL Technologies (Vancouver, Canada) and the classical PKC inhibitor Gö6976 was purchased from Merck (Darmstadt ...
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bioRxiv - Neuroscience 2023Quote: ... in mTeSR1 medium containing 10 μM Y-27632 ROCK inhibitor (STEMCELL Technologies) for the formation of embryoid bodies ...
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bioRxiv - Developmental Biology 2023Quote: ... and 10 µM ROCK-Inhibitor (Catalog no. Y-27632, Stem Cell Technologies). Organoids were maintained by hard splitting every week ...
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bioRxiv - Developmental Biology 2024Quote: ... supplemented with 50 µM Rock inhibitor Y-27632 (Stem Cell Technologies, 72302) were plated in 96 well V-bottom plate (low-binding ...
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bioRxiv - Cell Biology 2024Quote: ... using StemFlex media supplemented with 10 μM ROCK inhibitor (Stemcell Technologies 72304). We cultured the cells to 75% confluence ...
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bioRxiv - Cell Biology 2023Quote: ... with the addition of 1 μg/L of human interleukin 2 (IL-2, STEMCELL Technologies Inc., USA).
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bioRxiv - Cell Biology 2020Quote: T and B cell lineage depletion was performed using RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail (Stemcell Technologies) and Ficoll-Paque density gradient centrifugation from routine red cell exchange apheresis performed at Texas Children’s Hospital or Columbia University New York Presbyterian Hospital ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were enriched by Lymphoprep and RosetteSep Human CD4+ T Cell Enrichment Cocktail (both STEMCELL Technologies). CD4-enriched cells were then CD25-enriched using CD25 MicroBeads II (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... The minced tissue was then placed in an enzyme cocktail consisting of 1U/ml dispase (Stemcell Technologies, 07913), 2,000U/ml collagenase I (Worthington ...
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bioRxiv - Cancer Biology 2024Quote: ... CD3+ T-cell populations were isolated using RosetteSep Human T Cell Enrichment Cocktails (Stemcell Technologies, 15023 and 15022) and EasySep Human CD4 T Cell Iso Kit (Stemcell Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... gently layered onto room temperature Ficoll (Biosciences AB, Uppsala, Sweden Cat #:17-1440-03 Lot #: 10253776) in Sepmate-50 tubes (STEMCELL Technologies, Vancouver ...
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bioRxiv - Molecular Biology 2021Quote: PBMC were purified from blood samples from GD patients using Lymphoprep™ reagent and SepMate™ tubes (Stemcell Technologies, Vancouver, Canada). Lymphoprep™ was added to the lower compartment of the SepMate tube ...
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bioRxiv - Molecular Biology 2021Quote: ... the pooled samples were diluted 1:1 with PBS+2% FBS and loaded into SepMate-15™ tubes with 4.5 mL of Lymphoprep™ with a density of 1.077g/mL (StemCell Technologies). Cells were centrifuged at 1200x g for 20min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... PBMC and plasma samples were isolated after whole blood centrifugation 1200 g for 10 minutes at room temperature in SepMate tubes (STEMCELL Technologies). Plasma was stored at −80°C until analysis ...
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bioRxiv - Neuroscience 2021Quote: ... 2.5 mL of cell suspension was transferred to a sterile 5 mL round-bottom flow cytometry tube and placed in the EasySep magnet (STEMCELL Technologies) for 5 min ...
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bioRxiv - Immunology 2022Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
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bioRxiv - Immunology 2019Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors (Trima Apheresis, Blood Centers of the Pacific) by Ficoll centrifugation with SepMate tubes (STEMCELL Technologies). Bulk T cells were isolated from PBMCs using Easysep Human T Cell Isolation Kit (STEMCELL Technologies ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were collected from whole blood samples of healthy donors using SepMate™ tubes (STEMCELL Technologies, Canada) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: Peripheral blood mononuclear cells (PBMCs) were separated from whole blood samples of healthy donors using SepMate™ tubes (STEMCELL Technologies, Canada) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood using density gradient centrifugation with Lymphoprep in SepMate tubes (Stemcell Technologies) in a biosafety level 2+ facility according to the manufactory’s instruction ...
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bioRxiv - Immunology 2021Quote: PBMCs from three independent donors were isolated from leukapheresis products using Lymphoprep density gradient centrifugation and SepMate PBMC isolation tubes (StemCell Technologies), under the Weatherall Institute of Molecular Medicine ...
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bioRxiv - Immunology 2021Quote: ... Remaining blood was diluted with 8mL of DPBS and slowly added to a SepMate-50 tube pre-filled with 15mL of Lymphoprep (STEMCELL Technologies). The tube was then centrifuged at 800 rcf for 20 min with brakes off ...
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bioRxiv - Cell Biology 2020Quote: Peripheral blood was collected from a healthy volunteer into a BD Vacutainer tube and mononuclear cells were isolated using the SepMate kit (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... PBMC and plasma samples were isolated after whole blood centrifugation 1200 g for 10 minutes at room temperature in SepMate tubes (STEMCELL Technologies). Plasma was stored at -800C until analysis ...
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bioRxiv - Genetics 2022Quote: ... Peripheral blood and bone marrow mononuclear cells were isolated using Ficoll Paque Plus solution and density gradient centrifugation using SepMate tubes (StemCell Technologies). All samples were stored in vapor-phase liquid nitrogen after cryopreservation with 10% dimethyl sulfoxide until analysis ...
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bioRxiv - Developmental Biology 2023Quote: ... NMP-MSCs were suspended as a sphere in a 15-ml conical tube and cultured in MesenCult-ACF Chondrogenic Differentiation medium (STEMCELL Technologies) for 4 weeks.
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bioRxiv - Microbiology 2023Quote: ... from participants with HIV-1 on ART were isolated from whole blood by density gradient centrifugation using LymphoprepTM and SepMateTM tubes (StemCell Technologies), and cryopreserved ...
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bioRxiv - Immunology 2023Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin (R1) and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... A total volume of 10-20 ml of fresh blood was collected in EDTA separator tubes and peripheral blood mononuclear cells (PBMC) were immediately isolated using Lymphoprep and SepMate-50 (Stemcell Technologies) centrifugation tubes ...
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bioRxiv - Immunology 2023Quote: Different blood compartments (plasma, PBMCs, and RBCs) were separated by density gradient centrifugation using SepMate-15 tubes (STEMCELL Technologies, catalog #85415) to reduce blood processing time and user variability ...