Labshake search
Citations for Stemcell Technologies :
551 - 600 of 763 citations for Phosphatase Inhibitor Cocktail 2 Tubes 100X since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... and PINK1 p.I368N-2# (1067#) were cultured using mTeSR (STEMCELL Technologies, Catlog #85850) media on Matrigel (Corning ...
-
bioRxiv - Immunology 2023Quote: ... Isolated cells were cultured for 1-2 weeks in StemSpan Medium (Stemcell Technologies) supplemented with 10 ng/ml interleukin (IL)-3 ...
-
bioRxiv - Cancer Biology 2023Quote: ... the media was supplemented with 2 μg/ml heparin (STEMCELL Technologies; Cat #07980). When cells reached confluency ...
-
bioRxiv - Synthetic Biology 2023Quote: ... supplemented with Human Recombinant IL-2 (12.5 ng/mL) (Stemcell Technologies, Cat.#78036), with initial seeding at 1×106 alive cells/mL ...
-
bioRxiv - Synthetic Biology 2023Quote: ... supplemented with Human Recombinant IL-2 (12.5 ng/mL) (Stemcell Technologies, Cat.#78036), IL-7 (5 ng/mL ...
-
bioRxiv - Neuroscience 2023Quote: ... with 2 % (v/v) of SM1 Neuronal Supplement (5711, STEMCELL Technologies Inc., Germany) and 0,5 % (v/v ...
-
bioRxiv - Bioengineering 2024Quote: ... supplemented with 10 ng/mL of Human Recombinant IL-2 (STEMCELL TECHNOLOGIES, #78220) and ImmunoCult™ Human CD3/CD28 T Cell Activator (STEMCELL TECHNOLOGIES ...
-
bioRxiv - Neuroscience 2024Quote: ... Wash Solution 2 was composed of 96% BrainPhys Neuronal Medium (StemCell Technologies: 5790), 1X N2 (ThermoFisher ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2-3 × 106 hESCs suspended in the neural induction medium with the ROCK inhibitor Y27632 were seeded on AggreWell 800 plates (STEMCELL Technologies) for embryoid body (EB ...
-
bioRxiv - Cell Biology 2020Quote: ... Cell suspension-matrigel mixture was placed in a transwell and incubated in growth media with 10 μM ROCK inhibitor (Cat#72252; STEMCELL Technologies) in a 24 well plate for 48 hours ...
-
bioRxiv - Biochemistry 2019Quote: ... collected by 5 min centrifugation at 300 × g and resuspended in mTeSR1 supplemented with 10 µM Rock inhibitor Y-27632(2HCl) (72304, StemCell Technologies) for the first day.
-
bioRxiv - Developmental Biology 2022Quote: ... single hESCs were seeded into a spinner flask at a density of 1e6 cells/mL in mTeSR1 media containing 10 μM Rock inhibitor Y-27632 (STEMCELL Technologies) to allow formation of clusters ...
-
bioRxiv - Developmental Biology 2022Quote: ... Matrigel-coated wells of a 24-well plate with UPC medium supplemented with 10 µM Y-27632 ROCK inhibitor (STEMCELL Technologies) and incubated at 37°C and 5% CO2 ...
-
bioRxiv - Neuroscience 2020Quote: ... The iPSCs were subjected to monolayer Dual SMAD inhibition by changing the media to Neural Induction Media (NIM) which is composed of NBM supplemented with small molecules SB431542 (10μM, an inhibitor of TGFβ pathway) (72232, Stem Cell Technologies) and LDN193189 (0.1μM ...
-
bioRxiv - Developmental Biology 2021Quote: ... Transfected cells were resuspended in pre-warmed Essential 8™ medium supplemented with 10 µM ROCK-inhibitor (Y-27632) (Stemcell Technologies) and seeded into two wells of a Matrigel® coated 6-well plate ...
-
bioRxiv - Developmental Biology 2021Quote: ... Frozen vials of iPSC were thawed into mTeSR plus media supplemented with 10 µM ROCK inhibitor (Y27632, STEMCELL Technologies, catalog #72304) and the media changed after 2 days.
-
bioRxiv - Neuroscience 2022Quote: ... Harvested spheroids then were transferred into 10-cm ultra-low adhesion plates in Essential 6 medium containing dual SMAD pathway inhibitors [100 nM LDN193189 (STEMCELL Technologies) and 10 μM SB431542] and 2.5 μM XAV939 (Cayman) ...
-
bioRxiv - Cell Biology 2022Quote: ... and cultured in new 6-well plate using mTeSR™ plus medium supplemented with 10 μM Y-27,632 Rock inhibitor (StemCell Technologies). Pluripotency of all lines was established by analyzing the expressions of canonical stemness genes (SOX2 ...
-
bioRxiv - Developmental Biology 2022Quote: ... For HLC differentiation cells were seeded onto Matrigel™ coated 24-well tissue culture plates at 130,000 cells/ cm2 in mTeSR™ Plus containing 10 μM ROCK inhibitor (STEMCELL Technologies). After 24 hours the differentiation protocol was commenced using STEMdiff™ Definitive Endoderm Kit (STEMCELL Technologies ...
-
bioRxiv - Cell Biology 2020Quote: ... at 37℃ for 10 min and then were seeded on to a vitronectin-coated six-well plate at 3×105 cell/well in mTeSR1 supplemented with 5μM ROCK inhibitor (Y-27632, Stem Cell Technology) for 24 h ...
-
bioRxiv - Bioengineering 2021Quote: ... Cells (∼20 x 105 cells/cm2) were replated using the medium in which they were last cultured plus 5 μM ROCK inhibitor (#Y-27632, StemCell Technologies) and 5% Gibco™ KnockOut™ Serum Replacement (KSR ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were re-plated in mTeSR1 medium supplemented with 1% penicillin-streptomycin (Thermo Fischer Scientific) and 10 mM Rho-associated protein kinase (ROCK) inhibitor (Stemolecule Y-27632, STEMCELL Technologies) for 24 hr ...
-
bioRxiv - Neuroscience 2021Quote: ... Nucleofected cells were transferred to a new matrigel-coated well in a 6-well plate in 4mL of E8 medium containing 10μM ROCK inhibitor Y-27632 (StemCell Technologies #72307). Medium was replaced the following day with fresh E8 ...
-
bioRxiv - Neuroscience 2020Quote: ... when they reached high confluency and re-plated in mTeSR 1 with RHO/ROCK Pathway Inhibitor Y27632 (Stem Cell Technologies, 72302). Patient donors are identified with a code with one numeral (DEL_1 ...
-
bioRxiv - Bioengineering 2020Quote: ... the hiPSCs were collected and seeded at 1.5 × 105 cells/cm2 cell density on glass coverslips with Rho-associated coiled-coil containing protein kinase (ROCK) inhibitor supplemented mTeSR media (5 μM, StemCell Technologies). The culture was maintained with daily media changes until 90% confluency was reached ...
-
bioRxiv - Cell Biology 2022Quote: 6×107 hiPS cells were mixed with lentiviral Dolcetto library to achieve estimated 0.3 MOI and seeded on 2x Vitronectin coated 5-layer flasks at 36K cells/cm2 density in mTeSR-Plus medium with Rock Inhibitor (Ri, 10μM, Y-27632, Stemcell technologies, 72304). The next day ...
-
bioRxiv - Cell Biology 2023Quote: ... The detached colonies were then pelleted at 300g(av) and reseeded with mTeSR Plus medium supplemented with 10 µM Rock Inhibitor (Y-27632, STEMCELL Technologies). At passage 10 ...
-
bioRxiv - Genomics 2023Quote: ... human iPSCs were plated at cell density of 3.5×105 cells/well on Matrigel coated 12-well-plates using mTeSR™ medium supplemented with 5µM ROCK inhibitor (Y-27632, STEMCELL Technologies) for 24 h ...
-
bioRxiv - Bioengineering 2023Quote: ... and then incubated overnight in media supplemented with 5 µM of the Rho-associated protein kinase (ROCK) inhibitor Y-27632 (STEMCELL Technologies).
-
bioRxiv - Cell Biology 2024Quote: ... the cells were resuspended and 50,000-100,000 cells were transferred into fresh matrix-coated plates with mTeSR Plus media containing 10 μM Y-27632 (ROCK inhibitor, STEMCELL Technologies). Fresh media without ROCK inhibitor was fed the next ...
-
bioRxiv - Cancer Biology 2021Quote: ... heparin (2 mg/mL 0.2% Heparin Sodium Salt in PBS; STEMCELL technologies, Cat#07980), antibiotic-antimycotic (1X ...
-
bioRxiv - Neuroscience 2022Quote: ... supplemented with 1% N2 supplement and 2% NeuroCult S1 supplement (STEMCELL Technologies, Vancouver, BC). After one day media was removed and replaced with a thin layer of phosphate buffered saline ...
-
Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Cells were fed with 2 ml of HemA medium (HPC differentiation kit, StemCell Technologies) on day 0 and half-fed with 1 ml on day 2 ...
-
bioRxiv - Neuroscience 2021Quote: ... culture medium was replaced with 2 mL/well iHSC Medium A (StemCell Technologies #05310) on differentiation Day 0 ...
-
bioRxiv - Developmental Biology 2022Quote: ... 5 ng/mL FGF-2 (RnD Systems) and 1 µM CHIR99021 (Stem Cell Technologies) with daily medium exchange for 3 days ...
-
bioRxiv - Immunology 2020Quote: ... and 2.5μl/ml anti-CD3/23/2 ImmunoCult T cell activator (StemCell Technologies #10970). Medium was changed on day 3 and samples were assessed by flow cytometry and ELISA on day 8.
-
bioRxiv - Immunology 2019Quote: ... Spin EB differentiation medium: STEMdiff™ APEL™2 Medium (STEMCELL Technologies,Catalog # 05270).
-
bioRxiv - Genetics 2020Quote: ... HUDEP-2 cells were cultured in a base medium of SFEM (Stemcell Technologies 9650) containing to a final concentration of dexamethasone 1uM (Sigma D4902-100MG) ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 ng/ml FGF-2 (RnD Systems) and 1 μM CHIR99021 (Stem Cell Technologies). Mesoderm induction required daily medium exchanges for 3 days ...
-
bioRxiv - Immunology 2020Quote: ... + 10 ng/ml IL-15 and 10 IU of IL-2 (Stemcell Technologies, Canada). The culture media was replenished every three days with fresh media containing 10 IU of IL-2 and 10 ng/ml IL-15 ...
-
bioRxiv - Immunology 2022Quote: ... + 10 ng/ml IL-15 and 10 IU of IL-2 (Stemcell Technologies, Canada). The culture media was replenished every three days with fresh media containing 10 IU of IL-2 and 10 ng/ml IL-15 ...
-
bioRxiv - Bioengineering 2023Quote: ... Gentle cell dissociation reagent and APEL(2) Medium were purchased from Stem Cell Technologies. Primary antibodies used were mouse anti-Oct 3/4 (Santa Cruz Biotechnology – SC-5279) ...
-
bioRxiv - Developmental Biology 2023Quote: ... and colonies gently lifted by 2 min incubation in ReLeSR (Stem Cell Technologies, #05872) at 37°C ...
-
bioRxiv - Neuroscience 2023Quote: GNAO1+/G203R#2 hiPSCs were cultured in mTeSR™1 medium (Stem Cell Technologies) on Matrigel (Corning)-coated plates following the manufacturer’s guidelines ...
-
bioRxiv - Cell Biology 2024Quote: ... iPSCs were then treated with 2 ml pre-warmed Accutase (AT104, Stem Cell Technologies) and the vessel was incubated at 37ºC for 10 mins ...
-
bioRxiv - Cell Biology 2020Quote: ... at 37°C for 5 minutes and replated in a ratio of 1:6 or 1:9 on new Matrigel-coated plates in E8 supplemented with 5 μM ROCK inhibitor Y-27632 (Stemcell Technologies, Canada) for 24h ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were harvested with GCDR and plated in Essential-E8 supplemented with 10 uM Rho-kinase inhibitor Y27632 (Stem Cell Technologies, 72304) on VTN coated 6-well culture dish to yield approximately 80% confluence next day ...
-
bioRxiv - Neuroscience 2019Quote: ... 8 x 105 cells were resuspended in 100 μl Ingenio-solution (Mirrus) supplemented with Rock inhibitor (10 μM, Y-27632, Stem Cell Technologies) and 2.5 μg of CRISPR:hSpCas9 plasmid and 2.5 μg linearized DNA targeting plasmid and immediately pulse with program DC100 (using a 4D-Nucleofector ...
-
bioRxiv - Genetics 2021Quote: ... Cells were resuspended in 1 mL pre-warmed Neuronal Expansion Media with Rock inhibitor (Y-27632 compound, Stem Cell Technologies, cat# 72304) at a final concentration of 10 μM and a cell count performed ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cells were passaged in clumps of 8-10 cells and seeded in mTeSR supplemented with the Rho-associated protein kinase inhibitor γ-27632 (STEMCELL Technologies) at 10 µM for the first day to improve survival ...