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Citations for Stemcell Technologies :
4601 - 4650 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: iPSC derived astrocytic cells (hiAC) were differentiated from NPCs by small molecule differentiation in STEMdiff™ Astrocyte Differentiation Media (Stem Cell Tech #cat100-0013) on Corning® Matrigel® coated tissue culture treated plates ...
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bioRxiv - Bioengineering 2022Quote: ... were purchased from StemCell Technologies (Vancouver, CA). G418 (Cat ...
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bioRxiv - Bioengineering 2022Quote: ... 1 mL of gentle cell dissociation reagent (GDR) (Stem Cell Technologies) was added for 10-15 minutes ...
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bioRxiv - Cell Biology 2022Quote: CFU assays in PHZ-treated and retrovirally-infected spleen progenitors were conducted using spleen suspensions at 2 × 104 cells/mL mixed with Methocult M3434 (STEMCELL Technologies) containing Epo ...
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bioRxiv - Immunology 2022Quote: ... Total blood DCs were enriched with EasySep human pan-DC preenrichment kit (Stemcell Technologies 19251). DC enriched fraction were stained with antibodies specific for HLA-DR APCeFluor780 ...
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bioRxiv - Bioengineering 2022Quote: ... were either coated with 70 µL of 2% agarose in water or PBS or treated for 20 min with 100 µL of anti-adherence rinsing solution (StemCell Technologies 07010). The wells were washed with PBS ...
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bioRxiv - Neuroscience 2022Quote: ... medium was refreshed every 7 days by replacing half of the medium with Brainphys neuronal medium supplemented with 2% NeuroCult SM1 neuronal supplement (Stem cell technologies, Köln, Germany) and 1% pen/strep (BP-complete medium).
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bioRxiv - Neuroscience 2022Quote: ... supplemented with SM1 (Stem Cell Technologies, Vancouver, BC, Canada) and 0.5 mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were differentiated into hematopoietic progenitor cells (HPCs) using the STEMdiff Hematopoietic Kit (StemCell Technologies). Floating HPCs were then collected and plated at 50,000 cells/mL in STEMdiff Microglia Differentiation media (StemCell Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Floating HPCs were then collected and plated at 50,000 cells/mL in STEMdiff Microglia Differentiation media (StemCell Technologies) for 24 days ...
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bioRxiv - Neuroscience 2022Quote: ... followed by rapid maturation in STEMdiff Microglia Maturation media (StemCell Technologies) for at least 4 days ...
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bioRxiv - Neuroscience 2022Quote: Microglia were differentiated using the commerically available differentiation kit (STEMCELL Technologies #05310, #100-0019, #100-0020) based on a previously published protocol [39] ...
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bioRxiv - Neuroscience 2022Quote: hiPSCs were cultured at 37ºC and 5% CO2 in a humidified incubator (BINDER C150) in mTeSR1 medium (STEMCELL Technologies, #85850) on Matrigel (Corning ...
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bioRxiv - Immunology 2022Quote: ... Cells were plated at 1 × 106 cells/ml in T cell growth media (StemCell Technology, # 10981) and activated using ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (StemCell Technology ...
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bioRxiv - Immunology 2022Quote: ... and activated using ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (StemCell Technology, #10970) with or without 2µM of NU74411 for 48 hours.
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bioRxiv - Immunology 2022Quote: Mouse HSCs were isolated by negative selection from 5-8 week old female Balb/C mouse bone marrow using EasySep™ mouse hematopoietic progenitor cell isolation kit (Stemcell Technologies) following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Splenocyte-derived CD4+ T cells were isolated using Easysep Mouse CD4+ T cell isolation kit (19852, STEMCELL Technologies), and then 2e5 cells/well were plated in a 96 well plate ...
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bioRxiv - Immunology 2022Quote: ... Lm-OVA-specific CD8+ OT-I T cells were isolated from the spleen of infected mice by positive selection using the EasySep mouse CD90.1 positive selection kit (StemCell Technologies) as previously described (2 ...
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bioRxiv - Immunology 2022Quote: Splenic macrophages were magnetically labeled and isolated using StemCell EasySep PE Positive Selection Kit II in concert with an F4/80 PE-conjugated antibody (StemCell Technologies). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Mononuclear cells (MNCs) were enriched using Lymphoprep density gradient medium (Catalog #07811) and SepMate tubes (Catalog #85450; STEMCELL Technologies). Briefly ...
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bioRxiv - Immunology 2022Quote: ... CD14+ monocytes were magnetically isolated (#100-0694 EasySep human positive selection kit II, STEMCELL Technologies) and the resulting cell suspension was passed through a 70 μm cell strainer.
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bioRxiv - Immunology 2022Quote: ... naïve CD8+ T cells were purified from spleen and peripheral lymph nodes by negative selection (StemCell Technologies, Vancouver, BC) as previously described (2) ...
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bioRxiv - Immunology 2022Quote: ... eNK cells added to T cell cultures were removed (EasySep Human CD56 Positive Selection Kit II, STEMCELL Technologies) prior to analysis ...
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bioRxiv - Immunology 2022Quote: ... NK cells were either isolated from cryopreserved PBMCs by either negative selection using the EasySep NK cell enrichment kit (StemCell technologies) or by depletion of T cells with human CD3 MicroBeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... NK cells were removed from cultures (EasySep Human CD56 Positive Selection Kit II, StemCell Technologies). Remaining CD4 T cells were analyzed by flow cytometry to confirm removal of NK cells by staining for CD56 ...
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bioRxiv - Immunology 2022Quote: ... Total CD4 T cells from PBMCs were isolated using the EasySep Human CD4+ T cell enrichment kit (StemCell technologies). Prior to infection ...
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bioRxiv - Immunology 2022Quote: ... and CD8+ T cells were subsequently isolated using the Stemcell human CD8+ isolation kit (Stemcell Technology) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were isolated from splenocytes of OT-I mice using a magnetic bead isolation kit (Stemcell Technology) and cultured following a previously published protocol (44) ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells from spleens were negatively enriched using an EasySep™ Mouse CD8+ T Cell Isolation Kit (#19853) according to the manufacturer’s instructions (STEMCELL Technologies). Enriched CD8+ T cells (92% - 96% purity ...
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bioRxiv - Immunology 2022Quote: CD3ε+ T cells were isolated using negative selection Human T cell Isolation Kit (StemCell Technology, #17951). Cells were plated at 1 × 106 cells/ml in T cell growth media (StemCell Technology ...
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bioRxiv - Immunology 2022Quote: ... FACS sorting was performed on an Influx-2 on pre-enriched CD4+ T cells (enrichment using an EasySep CD4+ T cell isolation kit (StemCell Technologies)) ...
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bioRxiv - Immunology 2022Quote: ... was prepared by addition of 0.5ml of Anti-Adherence Rinsing Solution (STEMCELL Technologies, no 07010) and centrifuged at 3,000 x g for three minutes to remove bubbles from the microwells ...
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bioRxiv - Microbiology 2022Quote: ... 1% Penicillin/Streptomycin and 16 ng/ml hepatocyte growth factor (HGF, Stem Cell Technologies). When selecting cells transduced by lentivirus ...
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bioRxiv - Microbiology 2022Quote: ... and neutrophils wee purified using an antibody-based negative selection kit (Stem Cell Technologies). Purified neutrophils were >84% viable (using Zombie Violet ...
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bioRxiv - Microbiology 2022Quote: ... and medium in the basal chamber was replaced with PneumaCult ALI Maintenance Medium (StemCell Technologies), including Antibiotic/Antimycotic Solution (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... the cells were thawed and passaged once in PneumaCult-Ex Medium (StemCell Technologies, Cologne, Germany) and then seeded on transwell inserts (12-well plate ...
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bioRxiv - Neuroscience 2022Quote: ... The cell mixture underwent centrifugation at 200g for 10min and then cultured using NeuroCult proliferation medium (Cat#05702, Stem Cell Technologies, Vancouver, Canada). The cells were maintained at standard conditions in a humidified 37 C° incubator containing 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... NSPC cultures were then passaged every 7 days by enzymatic disaggregation with Accutase (cat# 7920, Stem Cell Technologies, Vancouver, Canada). The medium was supplemented with Neurocult proliferation supplement (cat# 05702 ...
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bioRxiv - Neuroscience 2022Quote: ... Heparin solution 2 μg/ml (cat# 07980, Stem Cell Technologies, Vancouver, Canada), EGF 20 ng/ml (Cat#315-09 ...
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bioRxiv - Neuroscience 2022Quote: ... The medium was supplemented with Neurocult proliferation supplement (cat# 05702, Stem Cell Technologies, Vancouver, Canada) at 9:1 ratio ...
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bioRxiv - Neuroscience 2022Quote: ... Transduced cells were dissociated with Accutase (StemCell Technologies) and plated onto Matrigel-coated plates in mTeSR1 (StemCell Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... 1x N2 supplement B (StemCell Technologies) and 0.3% dextrose (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... and 1:1000 ROCKi (StemCell Technologies), and were kept in a warm metal bath to facilitate the thawing ...
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bioRxiv - Neuroscience 2022Quote: ... human PDGF-AA (10 ng/ml) and PDGF-BB (10 ng/ml; Shenandoah) and heparin (2 ng/ml; Stem Cell Technologies).
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bioRxiv - Neuroscience 2022Quote: ... human PDGF-AA (10 ng ml−1) and PDGF-BB (10 ng ml−1; Shenandoah) and heparin (2 ng ml−1; Stem Cell Technologies). For all patient-derived cultures ...
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bioRxiv - Neuroscience 2022Quote: ... in embryoid body media consisting of complete StemFlex supplemented with 10 μM Y27632 (Stemcell Technologies), 50 ng/mL BMP-4 ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.5 μM Purmorphamine (PMA, Stem Cell Technologies). Clumps of cells were then transferred to 10 cm Petri dishes and cultured in suspension for six days on a shaker at 80 RPM at 37 °C/5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... medium was switched to BrainPhys (5790 STEMCELL Technologies) supplemented with 1% N2 (17502-048 Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... cAMP (1mM, 100-0244 Stemcell Technologies), Ascorbic acid (200 nM ...
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bioRxiv - Neuroscience 2022Quote: ... or mTeSR Plus (StemCell Technologies). Medium was replaced every other day and cells were passaged ~1:10 onto fresh Matrigel-coated vessels using ReLeSR (StemCell Technologies ...