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Citations for Stemcell Technologies :
4751 - 4800 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and cells were passaged once a week using Gentle Cell Dissociation reagent (StemCell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... human neuron-astrocyte co-cultures were maintained in neuron maintenance medium (1X BrainPhys Basal (StemCell Technology), 1X B27 with Vitamin A (ThermoFisher) ...
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bioRxiv - Bioengineering 2022Quote: ... and accutase were purchased from Stemcell Technologies (Cambridge, MA, USA). RPMI 1640 was purchased from Caisson Labs (Smithfield ...
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bioRxiv - Bioengineering 2022Quote: ... PBMCs were isolated using density gradient centrifugation in a 50mL SepMate tube (StemCell Technologies) following manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... at a density of 3,000,000 cells per well with 2mL of AggreWell Medium (STEMCELL Technologies) supplemented with Rho Kinase Inhibitor (Y-27632 ...
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bioRxiv - Bioengineering 2022Quote: ... aggregates were transferred onto a 37 µm filter (STEMCELL Technologies) by carefully aspirating with a p1000 wide-bore pipette out of the AggreWell plate ...
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bioRxiv - Bioengineering 2022Quote: ... and then aggregated in AggreWell 800 24-well plates (STEMCELL Technologies) at a density of 3,000,000 cells per well with 2mL of AggreWell Medium (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... High-density monolayers were cultured for 48 hours in mTeSR medium (STEMCELL Technologies) before induction (day 0 ...
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bioRxiv - Cell Biology 2022Quote: ... and resuspended in mTeSR-E8 media supplemented with 10μM Rock inhibitor (Ri) Y-27632 (Stemcell technologies, 72304). Prepare 0.5M cell/ml cell suspension in mTeSR+E8 +Ri media ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10μM Rock inhibitor (Ri) Y-27632 (Stemcell technologies, 72304). 0.5M cells were aliquoted and mixed with 200μl virus suspension and seeded on one well of 6 well-plate (in replicate) ...
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bioRxiv - Cell Biology 2022Quote: 6×107 hiPS cells were mixed with lentiviral Dolcetto library to achieve estimated 0.3 MOI and seeded on 2x Vitronectin coated 5-layer flasks at 36K cells/cm2 density in mTeSR-Plus medium with Rock Inhibitor (Ri, 10μM, Y-27632, Stemcell technologies, 72304). The next day ...
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bioRxiv - Cell Biology 2022Quote: hiPSC cells were harvested using accutase (Stemcell technologies, 07920) and resuspended in mTeSR-E8 Media (Stemcell technologies ...
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bioRxiv - Cell Biology 2022Quote: ... hIPSC lines were cultured on Vitronectin-XF (Stemcell Technologies, 07180) coated plates and in mTeSR-Plus Media (Stemcell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... coated plates and in mTeSR-Plus Media (Stemcell Technologies, 100-0276). For maintenance ...
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bioRxiv - Cell Biology 2022Quote: ... they were passaged every four days as clumps using ReLeSR (Stemcell Technologies, 05872) according to the producer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Bone marrow-derived GMPs from C57BL/6J mice were isolated by FACS sorting and cultured in Methocult GF M3434 (03434, Stem Cell Technologies) for 14 days ...
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bioRxiv - Cell Biology 2022Quote: ... and resuspended in mTeSR-E8 Media (Stemcell technologies, 05990) supplemented with 10μM Rock inhibitor (Ri ...
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bioRxiv - Cancer Biology 2022Quote: CD34-positive and Lin-negative cells from PBMC were single-cell sorted into 96-well plates containing 100 μL of free medium consisting of StemSpam SFEMII (Stemcell Technologies) with human cytokines SCF ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted in 2.25 mL of complete Basal Epicult media formulated according to manufacture instructions (Epicult Basal Medium Stemcell Technologies #05610 ...
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bioRxiv - Cancer Biology 2022Quote: ... glands were first digested overnight (∼16 hours) with gentle agitation at 37 °C in 250 μL Gentle Collagenase (Stemcell Technologies #07919) diluted in 2.25 mL of complete Basal Epicult media formulated according to manufacture instructions (Epicult Basal Medium Stemcell Technologies #05610 ...
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bioRxiv - Biochemistry 2022Quote: Peripheral blood mononuclear cells (PBMCs) were grown in StemSpan SFEMII supplemented media (STEMCELL Technologies, CA) which are distinct since they can be observed to float in culture ...
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bioRxiv - Bioengineering 2022Quote: ... until a final volume of 200 ml and layered per 25 ml on top of 10 ml LymphoprepTM (07851, StemCell technologies, Köln, Germany) in 50 ml centrifugal tubes ...
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bioRxiv - Cancer Biology 2022Quote: ... non-TC-treated 96-well U-bottom plates were treated with 100 μL of anti-adherence rinsing solution (StemCell Technologies 07010) for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... all the unwanted cells were labelled with magnet conjugated antibody cocktail which is separated using their EasySep magnetic stand leaving CD4 or CD8 cell in suspension using the vendor supplied EasySep Human CD4/CD8 T Cell Iso Kit (Stem Cell Technologies, 17952 for CD4 and 17953 for CD8). This negative selection approach results in isolation of untouched CD8 or CD4 T cells and stored frozen with 10% DMSO (MP Biomedicals ...
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bioRxiv - Cancer Biology 2022Quote: Primary T cells were isolated from LeukoPaks obtained from Stem Cell Technologies (200-0092). CD8 and CD4 cells were isolated separately using their EasySep™ Human CD8+/CD4+ T Cell Isolation Kit as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... AML patient-derived CD34+ leukemia stem cells (LSCs) were cultured in StemSpan SFEM (StemCell Technologies) supplemented with rhIL-3 (50 ng/mL) ...
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bioRxiv - Cancer Biology 2022Quote: ... using ALDEFLUOR (Stem Cell Technologies, Seattle, WA) according to the manufacturer’s instructions ...
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Synthetic lethal targeting of TET2-mutant hematopoietic stem and progenitor cells by XPO1 inhibitorsbioRxiv - Cancer Biology 2022Quote: ... Tet2 +/− and Tet2−/− mice were purified by flow cytometry and plated in methylcellulose medium (Methocult M3434, Stem Cell Technologies). Total number of colony-forming units (CFU ...
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bioRxiv - Cancer Biology 2022Quote: ... and SepMate tubes for density gradient centrifugation (Stemcell technologies). Cells were washed ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs were isolated from whole blood using lymphoprep (Stemcell technologies) and SepMate tubes for density gradient centrifugation (Stemcell technologies) ...
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bioRxiv - Cancer Biology 2022Quote: Cells were plated in methylcellulose medium (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... cells were dissociated with Accutase (CC# 07930, STEMCELL Technologies) and passaged into Matrigel- coated 10 cm culture plates for neural differentiation ...
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bioRxiv - Cancer Biology 2022Quote: ... Red blood cells were removed by immunomagnetic depletion using 50μL of EasySep RBC Depletion Reagent (Stemcell Technologies) per mL of sample according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8+ T cells were isolated using EasySep Human CD8+ T Cell Isolation Kit (Stemcell Technologies) by immunomagnetic negative selection according to manufacturer instructions.
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with NeuroCult™ Proliferation Supplement (STEMCELL Technologies), 20 ng/mL EGF (PeproTech Inc.) ...
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bioRxiv - Cancer Biology 2022Quote: ... The t(8;21)-positive patient primary cells were co-cultured on MSCs feeders and cultivated with SFEM II containing 1X Human Myeloid Expansion Supplement II (StemCell Technologies).
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bioRxiv - Cancer Biology 2022Quote: ... We grew cells in a 5% CO2 incubator in a serum-free NeuroCult NS-A basal medium (STEMCELL Technologies) supplemented with NeuroCult™ Proliferation Supplement (STEMCELL Technologies) ...
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bioRxiv - Biophysics 2022Quote: ... and then acclimated to the mTeSR1 culture medium (ST-85850, STEMCELL Technologies) until differentiation began ...
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bioRxiv - Cancer Biology 2022Quote: ... purified cells were incubated overnight in StemSpan (STEMCELL Technologies) supplemented with 100 ng/μl each of human SCF ...
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bioRxiv - Cancer Biology 2022Quote: ... and transferred in Methocult SF H4436 (Stem cell technologies) for 5 to 7 days.
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bioRxiv - Cancer Biology 2022Quote: CFC assays from ES and iPS cells from EB or BL-CFC were plated at 10×10^3 cells/mL into MethoCult GF (H4435 StemCell Technologies). Hematopoietic CFC was counted at day 19.
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bioRxiv - Cancer Biology 2022Quote: ... 10 ng/ml IL-3 (STEMCELL Technologies; Cat. No. 78042) was directly added to the medium to support growth in the absence of an oncogene ...
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bioRxiv - Cancer Biology 2022Quote: Tumours and matched lung metastases were dissociated into single cells using mechanical disruption followed by enzymatic digestion by a collagenase-hyaluronidase solution (StemCell Technologies; 07912) for 1 h at 37°C without mechanical agitation ...
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bioRxiv - Cancer Biology 2022Quote: ... 500 cells were seeded in 35-mm dish in methylcellulose media (H4435, Stem Cell Technologies, Vancouver, Canada). Colonies were photographed and scored 14 days later using ImageJ software (LOCI ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 nM hydrocortisone (STEMCELL Technologies; Cat. No. 07926), 30 nM sodium selenite (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... and FLT3-L (StemCell Technologies) and 1% penicillin/streptomycin to drive the differentiation to mature myeloid cells ...
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bioRxiv - Cancer Biology 2022Quote: ... and the hematopoietic progenitors were isolated by magnetic negative selection (Mouse Hematopoietic Progenitor Cell Isolation Kit, StemCell Technologies), and cultivated in SFEM II media supplemented with 50ng/ml of murine IL6 ...
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bioRxiv - Cancer Biology 2022Quote: ... EpiCult™ Plus Medium (STEMCELL tech) + 50 mg ml-1 Hydrocortisone (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated for 14 days and read on StemVision (Stem Cell Technologies) for the final colony count.
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bioRxiv - Cancer Biology 2022Quote: ... and EasySepTM Magnet (StemCell Technologies, Cat# 18000). If the cell pellet appeared red ...