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Citations for Stemcell Technologies :
4451 - 4500 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... The inhibitors and activin (STEMCELL Technologies 78001) are used at optimized concentration (10uM for A 83-01 and CPTH6 ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Cells were passaged every 2-3 days using accutase (Stemcell Technologies, #07920). Cells were then collected by centrifugation at 1200 rpm for 3 minutes and counted before seeding ...
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bioRxiv - Developmental Biology 2022Quote: ... NPC-iPS cells were treated with 10 µM Rho kinase inhibitor Y-27632 overnight before dissociated to single cells with Accutase (Stem Cell Technology 07922). About 10 million cells were electroporated with 10 μg of AAVS1 zinc finger nuclease-encoding plasmid and 30 μg of AAVS1-tdTomato with one electroporation of 250 V ...
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bioRxiv - Developmental Biology 2022Quote: ... organoids were treated from days 3 - 5 with ROCKi-free neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 μM and smoothened agonist (SAG ...
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bioRxiv - Cell Biology 2022Quote: ... hPSC-CMs were dissociated using STEMdiff Cardiomyocyte Dissociation Medium (Stemcell Technologies) for 15 min at 37℃ ...
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bioRxiv - Cell Biology 2022Quote: ... positive immunomagnetic selection of CD45+ cells were performed with EasySep Human CD45 Depletion Kit II (StemCell Technologies, Canada) according to the manufacturer instructions (post-isolation purity 96-99%) ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse CD8+ T cells were isolated from the mouse spleens using EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19853A) according to manufacturer instructions ...
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bioRxiv - Pathology 2022Quote: ... and 5 µM SB431542 (StemCell Technologies). On Days8-14 ...
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bioRxiv - Pathology 2022Quote: ... 2 µM CHIR99021 (StemCell Technologies), and 10 ng/mL Recombinant Human BMP-7(Peprotech) ...
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bioRxiv - Pathology 2022Quote: ... Then the cells were dissociated with Accutase (Stemcell Technologies) and plated onto low attachment V-type 96-well plate at 20 000 cells per well or AggreWell800 plate (Stemcell Technologies ...
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bioRxiv - Physiology 2022Quote: ... Human iPSCs were grown in mTeSR1 basal medium supplemented with mTeSR1 5x supplement (StemCell Technologies, UK) and 100 U/mL penicillin/streptomycin on cell culture plates coated with DMEM/F12 (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... at an optimised density of 0.8-1.3×106 cells/well in mTeSR1 supplemented with 10 µM Y-27632 (StemCell Technologies, UK). Once cells had attached (6+ hours) ...
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bioRxiv - Pathology 2022Quote: ... 1 IU/mL Heparin (StemCell Technologies), 100 nM LDN193189 (StemCell Technologies) ...
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bioRxiv - Pathology 2022Quote: ... 200 µM Ascorbic Acid (StemCell Technologies), and 1µM Dibutyryl-cAMP (StemCell Technologies).
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bioRxiv - Pathology 2022Quote: ... and disassociated by ReLeSR™ (Stemcell Technologies). After washed with fresh stem cell medium with 10 µM Y-27632 ...
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bioRxiv - Pathology 2022Quote: ... and plated onto low attachment V-type 96-well plate at 20 000 cells per well or AggreWell800 plate (Stemcell Technologies) to form aggregates ...
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bioRxiv - Pathology 2022Quote: ... iPSCs were pretreated with 50 μM Y-27632 (Stemcell Technologies) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... NPCs were first generated using STEMdiffTM SMADi Neural Induction kit (Stemcell technologies) and generated neural progenitor cells were expanded using STEMdiff TM Neural Progenitor Medium ...
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bioRxiv - Neuroscience 2022Quote: ... or mTESR+ medium (StemCell Technologies) with 100 U/mL penicillin and 100µg/ml streptomycin ...
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bioRxiv - Neuroscience 2022Quote: ... BP (LN): BrainPhys Neuronal Medium (Stem Cell Technologies), 2% B27+A (50X ...
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bioRxiv - Genetics 2022Quote: ... individual iPSC colonies were picked manually and transferred to Matrigel (Corning)-coated maintained in mTeSR medium (Stem Cell Technologies). Cells were tested routinely to be mycoplasma-negative ...
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bioRxiv - Systems Biology 2022Quote: ... with mTeSR Plus (Stem Cell Technologies, 100-0275) and supplemented with penicillin/streptomycin (P/S ...
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bioRxiv - Systems Biology 2022Quote: ... Organoids were washed three times with HBSS without Ca2+/Mg2+ (STEMCELL technologies, 37250). Single cell suspensions were obtained by adapting the protocol described in (Cowan et al. ...
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bioRxiv - Systems Biology 2022Quote: ... Stem cell lines were cultured in mTeSR1 (Stem Cell Technologies, 05851) with mTeSR1 supplement (Stem Cell Technologies ...
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bioRxiv - Systems Biology 2022Quote: ... with mTeSR1 supplement (Stem Cell Technologies, 05852) or mTeSR Plus (Stem Cell Technologies ...
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bioRxiv - Physiology 2022Quote: ... added into the methylcellulose culture medium (StemCell Technologies, MethoCult™ M3334 ...
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bioRxiv - Systems Biology 2022Quote: ... or mTeSR Plus (Stem Cell Technologies, 100-0274) with mTeSR Plus (Stem Cell Technologies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... passaged once in PneumaCult-Ex Medium (StemCell technologies), and seeded on transwell inserts (12-well plate ...
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bioRxiv - Immunology 2022Quote: Primary human CD8+ T cells were isolated from buffy coats of healthy donors and purified (>95%) by negative selection using the RosetteSep™ Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies, #15063), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Infectious units per million were estimated by limiting dilution statistics using L-Calc™ Limiting Dilution Software (STEMCELL Technologies).
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were harvested from Leukocyte Reduction System (LRS) Cones provided by STEMCELL Technologies and cryopreserved ...
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bioRxiv - Immunology 2022Quote: ... erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep™ Rat CD4+ T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting ...
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bioRxiv - Immunology 2022Quote: 5e4 total bone marrow cells and 2.5e5 total splenocytes were plated in Methocult media M3434 (StemCell Technologies) supplemented with hemin ...
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated using the density gradient centrifugation method (GE Healthcare, STEMCELL Technology). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Primary human CD8+ were isolated from healthy donor blood with commercially available negative selection kits (RosetteSep Human CD8+ T cell Enrichment Cocktail, STEMCELL technologies, UK) following the manufacturers instruction ...
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bioRxiv - Immunology 2022Quote: ... CD4+ Human T cells were enriched from PBMCs using EasySep™ Human CD4+ T Cell Isolation Kit (StemCell Technologies) according to the manufacturers protocol ...
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bioRxiv - Immunology 2022Quote: Untouched PMNs were isolated from bone marrow in donor mice of the indicated genotype (age 6–8 wk) using EasySep mouse neutrophil enrichment kit (Stemcell Technologies Inc.). In a subset of experiment PMNs from C57/J animals were incubated with 1 mM Dimethyloxalylglycine (DMOG ...
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bioRxiv - Genomics 2022Quote: ... iPSCs were dissociated with Gentle Cell Dissociation Reagent (StemCell Technologies) and cultured on with E8 medium supplemented with 5 μM Y-27632 (SelleckChem) ...
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bioRxiv - Immunology 2022Quote: mLN DCs were isolated and purified (CD11c Positive Selection Kit II, STEMCELL Technologies) 2 days after inoculation of WT mice (T ...
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bioRxiv - Immunology 2022Quote: ... successfully transduced cells were enriched based on CD19t expression on day 11-15 using EasySep™ Human CD19 Positive Selection Kit II (Stemcell Technologies, Inc., Cat#17854), and the resulting CAR+ T cells were cryopreserved at day 15 or 17 ...
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bioRxiv - Immunology 2022Quote: ... Naive human CD4+ T cells were isolated using the EasySep Human Naïve CD4 TCell Iso Kit II (Stem Cell Technologies) according to the manufacturers’ instructions ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... CD3-CD19- mononuclear cells were enriched by Lymphoprep (Stem Cell Technologies, Grenoble, France) density gradient centrifugation followed by Magnetic-activated cell sorting using biotin MicroBead kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: Primary B cells were purified using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies) and stimulated with anti-IgM (20µg/mL ...
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bioRxiv - Immunology 2022Quote: ... Naïve splenic B lymphocytes were purified by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies). Primary B cells from each transgenic mouse line were cultured in RPMI 1640 with L-glutamine supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2022Quote: ... NK cells were isolated using the EasySep Human NK Cell Isolation Kit (Stem Cell Technologies) following manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells were isolated by negative magnetic selection from either B*08:01+ or B*35:01+ donor blood using kits from StemCell Technologies (catalog #17953). To screen for antigen-specific CTLs ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were enriched using an EasySep magnetic negative selection kit (Stem Cell Technologies) as per the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were enriched using an EasySep magnetic negative selection kit (Stem Cell Technologies #19853) as per the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... Immunomagnetic negative selection (Easy Sep NHP, STEMCELL Technologies, Cambridge, MA) was used to enrich in CD4+ or CD8+ T cells from PBMCs that were cultured in X-vivo 15 media (Lonza ...
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bioRxiv - Immunology 2022Quote: ... Enriched CD4+ and CD8+ T cells were activated with ImmunoCult NHP CD2/CD3/CD28 T-cell activator (STEMCELL Technologies) and incubated 3 days at 37°C in humidified 5% CO2.