Labshake search
Citations for Stemcell Technologies :
4851 - 4900 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... monocytes were purified using a CD14 positive selection kit (StemCell Technologies, Cat # 17858). Monocytes (3.6 × 106 cells ...
-
bioRxiv - Cell Biology 2022Quote: hematopoietic progenitors (CD34+CD43+ for iPSC and CD34+ for primary cells) were plated in human methylcellulose medium H4434 (STEMCELL technologies), containing recombinant human cytokines and scored for the presence of colonies 14 days after.
-
bioRxiv - Cell Biology 2022Quote: ... DTA or NNMTi iPS cells were resuspended to single cells and seeded into well of Aggrewell 400 plate (Stemcell Technologies #34415) in 1 mL of complete blastoid media ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in mtesR-plus medium with 10 µm Y-27632 (STEMCELL Technologies, Inc.) for 24 h before this was replaced with complete MTesR plus medium.
-
bioRxiv - Cell Biology 2022Quote: Antibodies and fluorochromes used: Mouse hematopoietic progenitor cell isolation cocktail (Stem Cell Technologies 19856), CD48-APC (Thermo-Fischer 17-0481-82) ...
-
bioRxiv - Cell Biology 2022Quote: ... Red blood cells were removed using the ammonium chloride solution: 5 ml of 0.8% Ammonium Chloride (StemCell Tech. 07800) was added to the suspension and incubated for 10 min with intermittent mixing ...
-
bioRxiv - Cell Biology 2022Quote: ... lineage depletion was performed as follows: added 20 µl of the EasySep mouse hematopoietic progenitor cell isolation cocktail (Stem Cell Technologies 19856), incubated for 15 min ...
-
bioRxiv - Cell Biology 2022Quote: ... or with Accutase (both obtained from STEMCELL Technologies, Inc.). Then ...
-
bioRxiv - Cell Biology 2022Quote: ... which comprised of StemSpan SFEM II medium with Stemspan CD34+ supplement (STEMCELL Technologies, Inc., Vancouver, Canada). Cells were maintained for 4 days at 5% CO2 at 37°C before further use.
-
bioRxiv - Cell Biology 2022Quote: ... 800ul of cleared media or cell extracts were mixed with 200ul of OptiPrep Density Gradient Medium (Stemcell Technologies: 7820). 900ul of the mixture were transferred to a polycarbonate ultracentrifuge tubes (Beckman Coulter:343778) ...
-
bioRxiv - Developmental Biology 2022Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
-
bioRxiv - Developmental Biology 2022Quote: ... HUES8 was maintained using mTeSR1 (Stem Cell Technologies, 85850) in 500ml spinner flasks (Corning ...
-
bioRxiv - Developmental Biology 2022Quote: Single cell suspension of the hPGCLC differentiation culture was generated by incubating the cells with Accutase (Stem Cell Technologies) for 15 min at 37°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... We have obtained comparable hPGCLC differentiation using 10µM Y-27632 (Stem Cell Technologies) instead of RevitaCell ...
-
bioRxiv - Developmental Biology 2022Quote: ... and suspended in mTeSR plus (Stemcell Technologies, 100-0276) medium containing 10μM Y27632 ROCK inhibitor (Axon Medchem ...
-
bioRxiv - Developmental Biology 2022Quote: H9 hESCs were cultured in mTESR-1 medium supplemented with 10mM ROCKi (Y27632; StemCell Technology 72304) for 24hrs ...
-
bioRxiv - Developmental Biology 2022Quote: ... Dispase II at 5 U/ml stock solution in Hanks’ balanced saline was from Stemcell Technologies (cat. no. 07913). The following chemicals were prepared and used according to the manufacturers’ guidelines ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged 1:6 using RELESR (StemCell Technologies 05872) for detachment of hESCs ...
-
bioRxiv - Developmental Biology 2022Quote: ... using mTESR-1 medium (StemCell Technologies 85850) and grown for 5-8 days with daily medium replacement ...
-
bioRxiv - Cell Biology 2022Quote: ... and ectodermal lineage we used the STEMdiff Trilineage Differentiation Kit (Stemcell Technologies, Vancouver, Canada), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... The iPSC lines were cultured on tissue culture plastic coated with vitronectin (0.5 μg/cm2; Stemcell Technologies, Vancouver, Canada) in StemMACS iPS-Brew XF (Miltenyi Biotec GmbH ...
-
bioRxiv - Developmental Biology 2022Quote: ... the cells were transferred into STEMdiffTM Neural Progenitor Medium (Stem Cell Technologies, #05833) for expansion and freezing.
-
bioRxiv - Developmental Biology 2022Quote: ... and mesoderm using the STEMdiffTM Trilineage Differentiation Kit (Stem Cell Technologies, #05230) according to the manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2022Quote: ... in STEMdiffTM Neural Progenitor Medium (Stem Cell Technologies, #05833).
-
bioRxiv - Developmental Biology 2022Quote: H9 hESCs were differentiated into early neural progenitor cells (eNPCs) with STEMdiffTM Neural Induction Medium (Stem Cell Technologies, #05835) following the manufacturer’s instructions for the monolayer protocol variant ...
-
bioRxiv - Developmental Biology 2022Quote: KOLF2 iPS cells were grown under feeder-free conditions on Matrigel-coated plates in TeSR™-E8™ (Stem Cell Technologies, #05990).
-
bioRxiv - Developmental Biology 2022Quote: ... the cells were dissociated and plated at a density of 3×104 cells/cm in STEMdiff™ Neuron Maturation Kit (Stem Cell Technologies, #08510) medium on a double layer of poly-L-ornithine (Sigma-Aldrich #P3655 ...
-
bioRxiv - Developmental Biology 2022Quote: Cells were passaged around every five days at 80-90% confluence using Accutase (Stem Cell Technologies, #07920) for detaching and DMEM/F-12 + GlutMAX (Invitrogen ...
-
bioRxiv - Developmental Biology 2022Quote: ... by plating them at 125.000 cells/cm2 on Matrigel and growing the cells in STEMdiff™ Neuron Differentiation Kit (Stem Cell Technologies, #08500) medium for one week with daily medium changes.
-
bioRxiv - Developmental Biology 2022Quote: ... and frozen in STEMdiffTM Neural Progenitor Freezing Medium (Stem Cell Technologies, #05838).
-
bioRxiv - Developmental Biology 2022Quote: ... in mTeSR™1 medium (Stem Cell Technologies, #85850). Medium changes were performed daily ...
-
bioRxiv - Developmental Biology 2022Quote: Embryoid body (EB) formation from hESCs (H1 line) were performed using Aggrewell™800 plates (#27865, StemCell Technologies Inc.) and APEL2 medium (#05270 ...
-
bioRxiv - Developmental Biology 2022Quote: ... iPSCs were routinely passaged with acctuase (# 07920, StemCell Technologies Inc.), while hESCs were routinely passaged with collagenase (#07909 ...
-
bioRxiv - Developmental Biology 2022Quote: ... while hESCs were routinely passaged with collagenase (#07909, StemCell Technologies Inc.). Cells were routinely tested for mycoplasma using the MycoAlert Mycoplasma Detection Kit from Lonza (LT07-118).
-
bioRxiv - Developmental Biology 2022Quote: Neural conversion of iPSCs was performed using STEMdiff™ Neural Induction Medium (#05831, StemCell Technologies Inc.) in combination with a monolayer culture method according to the manufacturer’s technical bulletin (#28044) ...
-
bioRxiv - Developmental Biology 2022Quote: ... (#05871/05852, StemCell Technologies Inc.). iPSCs were routinely passaged with acctuase (# 07920 ...
-
bioRxiv - Developmental Biology 2022Quote: ... single cell suspensions were prepared by treatment of hES cells with accutase (#07920, StemCell Technologies Inc.) and 1 × 106 cells were seeded per well of an Aggrewell™800 plate in APEL2 medium supplemented with 10 μM Y-27632 ROCK inhibitor (#72307 ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 1 × 106 cells were seeded per well of an Aggrewell™800 plate in APEL2 medium supplemented with 10 μM Y-27632 ROCK inhibitor (#72307, StemCell Technologies Inc.) followed by 72h incubation with daily media change ...
-
bioRxiv - Developmental Biology 2022Quote: ... and APEL2 medium (#05270, StemCell Technologies Inc.) following the guidelines of the manufacturer’s technical manual (#29146) ...
-
bioRxiv - Developmental Biology 2022Quote: ... cells were plated at single-cell density to obtain colonies of single clones using CloneR (Stemcell Technologies)-supplemented media ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 10nM LDN193189 (StemCell Technologies). The neural induction medium was replaced daily for 7 days ...
-
bioRxiv - Cell Biology 2022Quote: ... or A83-01 (100 nM; Stemcell Technologies, Vancouver, Canada) (Tojo et al. ...
-
bioRxiv - Cell Biology 2022Quote: ... and 1x CloneR (Stemcell Technologies, Vancouver, Canada) on laminin 521 (5 μg/mL ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were cultured in the presence of 3 μM CHIR-99021 (Stem Cell Technologies). Then cells were cultured for 1 day without cytokines ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were harvested and frozen at −150°C in CryoStor® CS10 cell freezing medium (StemCell Technologies). Cell count and viability were evaluated using NC-200 NucleoCounter (Chemometec) ...
-
bioRxiv - Cell Biology 2022Quote: ... in mTeSR1™ medium (StemCell Technologies) at 37°C in a 5% CO2 incubator with daily media changes and were passaged every 4-5 days using TrypLETM (ThermoFisher) ...
-
bioRxiv - Cell Biology 2022Quote: ... based enzymatic dissociation method was employed for passaging and 10 µM Y-27632 (Stem Cell Technologies, 72307) was supplied on the first day of each passage.
-
bioRxiv - Cell Biology 2022Quote: ... they were cultured during 24 hours in the presence of 10 μM of Rock inhibitor Y-27632 (StemCell Technologies).
-
bioRxiv - Developmental Biology 2022Quote: ... medium was gradually switched to Brainphys neuronal medium (Stemcell technologies, cat. no. 05790) and supplemented with BDNF (20 ng/ml ...
-
bioRxiv - Developmental Biology 2022Quote: ... the AggreWell-400 (STEMCELL Technologies) was prepared according to the manufacturer’s instructions ...