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Citations for Stemcell Technologies :
4501 - 4550 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... after blood samples were diluted 1:1 with PBS during B cell isolation using RosetteSep Human B-Cell Enrichment Cocktail (Stem Cell Technologies). SARS-CoV-2 RBD-specific IgG ELISA tests with the plasma were performed in the lab of Dr ...
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bioRxiv - Immunology 2022Quote: CD34+ cells were isolated from human cord blood (Stemcell Technologies #17896) and cultured for 2 days in expansion media (Miltenyi #130-100-473) ...
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bioRxiv - Microbiology 2022Quote: ... 100 µL of 100 µg/mL of DNase 1 (STEMCELL technologies, Canada) was added to 0.5 mL of stationary phase culture ...
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bioRxiv - Microbiology 2022Quote: ... 100 µL of 100 µg/mL of DNase 1 (STEMCELL technologies, Canada) was added to 0.9 mL of liquid culture (incubated for 3.7 h ...
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bioRxiv - Immunology 2022Quote: ... until a final volume of 200 ml and layered per 25 ml on top of 10 ml Lymphoprep™ (07851, StemCell technologies, Köln, Germany) in 50 ml centrifugal tubes ...
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bioRxiv - Immunology 2022Quote: ... gradient centrifugation using SepMate-50 tubes (Stemcell Technologies Inc) following the manufacturer’s protocol ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... Dzierzak (Erasmus University MC, Rotterdam, The Netherlands) and maintained in 50% Myelocult M5300 medium (Stem Cell Technologies, Grenoble, France), 35% α-MEM ...
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bioRxiv - Immunology 2022Quote: ... and 30 μl of additional biotinylated anti-CD3 (STEMCELL Technologies, 18051) was added to the sample to improve purity ...
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bioRxiv - Immunology 2022Quote: ... NK cells were then isolated from these PBMCs using a magnetic negative selection kit (STEMCELL Technologies, 17955). Briefly ...
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bioRxiv - Immunology 2022Quote: ... CD19+ cells were purified from C57BL/6 wild-type mice using the EasySep magnetic separation system (Stemcell Technologies, Vancouver, Canada) and were activated with lipopolysaccharide (10 μg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... AP positive colonies were then manually picked and seeded for further expansion onto Matrigel-coated wells of 24-well plates with mTeSR-1 (STEMCELL Technologies) maintenance medium with 10 µM Y-27632 ROCK inhibitor ...
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bioRxiv - Developmental Biology 2022Quote: ... medium was replaced by 1 ml of a 1:1 mixture of UPC medium and reprogramming medium TeSR-E7 (STEMCELL Technologies). From day 4 to day 18-20 ...
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bioRxiv - Immunology 2022Quote: ... These cells were then cultured in MethoCult medium (M3231, Stem Cell Technologies) supplemented with 10 ng/ml GM-CSF ...
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bioRxiv - Immunology 2022Quote: ... All CD19+ B cells were isolated (STEMCELL Technologies EasySep Mouse B Cell Isolation kit ...
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bioRxiv - Immunology 2022Quote: ... All CD19+ B cells were isolated (STEMCELL Technologies EasySep Mouse B Cell Isolation kit, STEMCELL Technologies) and mRNA was extracted and purified (PureLink RNA Mini Kit ...
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bioRxiv - Immunology 2022Quote: ... Mononuclear cells were purified by using SepMate™ (Stemcell technologies, Cambridge, MA, USA) using FICOLL ...
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bioRxiv - Cell Biology 2022Quote: ... with feeder-free mTeSR™1 maintenance medium (Stemcell Technologies, 85850). The enzyme-free Gentle Cell Dissociation Reagent (Stemcell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... The enzyme-free Gentle Cell Dissociation Reagent (Stemcell Technologies, 100-0485) was used to dissociate iPSCs into cell aggregates for routine passaging.
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bioRxiv - Immunology 2022Quote: ... Human PBMCs were isolated by using Lymphoprep (Stemcell Technologies, Vancouver, BC, Canada) gradient centrifugation ...
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bioRxiv - Cancer Biology 2022Quote: The ALDEFLUOR kit (StemCell Technologies, Durham, NC, USA) was used to analyze the population with high ALDH enzymatic activity according to the manufacturer’s intructions ...
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bioRxiv - Genetics 2022Quote: ... Anti-nestin mouse antibody (StemCell Technologies, 60091, 1:1000), Alexa Fluor 488-anti-Mouse (A11029 ...
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bioRxiv - Genetics 2022Quote: ... Control and deletion NPCs were grown in STEMdiff™ Neural Progenitor Medium (StemCell Technologies, Vancouver, Canada) on PDL/laminin coated plates prior to harvesting ...
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bioRxiv - Genetics 2022Quote: ... iPSCs were treated with 1 U/ml dispase (StemCell Technologies) and transferred to uncoated T-25 flasks in mTESR media supplemented with 10 μM ROCK inhibitor ...
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bioRxiv - Genetics 2022Quote: ... neuronal differentiation media was switched after 2 weeks to BrainPhys™ Neuronal Medium (Stem Cell Technologies) supplemented with the same factors to promote optimal neuronal activity and maturation.
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bioRxiv - Genetics 2022Quote: ... neural rosettes were selected using the STEMdiffTM Neural Rosette Selection Reagent (Stemcell Technologies) and replated on new coated plates ...
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bioRxiv - Genetics 2022Quote: ... STEMdiff™ Neural Induction Medium + SMADi (Stemcell Technologies) was used per manufacturer instructions to generate NPCs from embryoid bodies (EB) ...
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bioRxiv - Genetics 2022Quote: ... AggrewellTM plates were treated with Anti-Adherence Rinsing solution (Stemcell Technologies) as per manufacturer instructions ...
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bioRxiv - Genetics 2022Quote: ... the iPSCs were dissociated into a single-cell suspension with Accutase (StemCell Technologies) and resuspended in FACS buffer (D-PBS with 10 μM ROCK inhibitor) ...
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bioRxiv - Genetics 2022Quote: ... iPSCs were enzymatically dissociated using Accutase (Stemcell Technologies). 5 – 6×10^6 iPS were seeded per single well of an AggreWellTM 6-well plate ...
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bioRxiv - Genetics 2022Quote: ... and harvested and replated on day 6 on matrix-coated tissue culture plates and cultivated for 7 days in STEMdiffTM Neural Induction Medium (Stemcell Technologies) with daily medium changes ...
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bioRxiv - Genetics 2022Quote: ... 5µM SB431542 (StemCell Technologies) and 0.25µM LDN-193189 HCl (StemCell Technologies) ...
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bioRxiv - Genetics 2022Quote: ... and 0.25µM LDN-193189 HCl (StemCell Technologies). On day 3 ...
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bioRxiv - Genetics 2022Quote: ... AggreWellTM plates (Stemcell Technologies) were used ...
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bioRxiv - Cancer Biology 2022Quote: ... Splenocytes derived from OT1 mice were prepared as described above and naïve CD8+ T cells were isolated using EasySepTM Mouse Naïve CD8+ T cell isolation kit (STEMCELL Technologies) following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... the whole BM samples were incubated with Human B RosetteSep (Stemcell Technologies, CAN) following the manufacturer ‘instructions.
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bioRxiv - Cell Biology 2022Quote: ... in complete mTeSR media (StemCell Technologies). They were split every five days or when colonies began to merge ...
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bioRxiv - Cancer Biology 2022Quote: ... single-cell suspensions were depleted of lineage-committed hematopoietic cells using a Lineage Cell Depletion Kit according to manufacturer’s protocol (EasySep™, StemCell Technologies, Inc.). Lineage-depleted bone marrow was then stained with an antibody cocktail comprised of a lineage cocktail (BioLegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... and the EasySep Mouse NK Cell Isolation Kit (StemCell Technologies, cat no. 19855) was used to isolate NK cells through negative selection ...
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bioRxiv - Cancer Biology 2022Quote: ... Each well had 1 mL StemSpan SFEM (StemCell Technologies, Inc.) with 20 ng/mL recombinant murine SCF (PeproTech ...
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bioRxiv - Cancer Biology 2022Quote: ... RBC-lysed 50,000 whole bone marrow cells were seeded in 1.5mL MethoCult M3434 (Stem Cell Technologies) with no additional supplemental cytokines in triplicate on 6 well plates and scored on day 8 ...
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bioRxiv - Cancer Biology 2022Quote: ... single-cell suspensions were depleted of lineage-committed hematopoietic cells using a Lineage Cell Depletion Kit according to manufacturer’s protocol (EasySep™, StemCell Technologies, Inc.). 2.5-3.0 × 106 lineage-depleted bone marrow was then washed in PBS and then resuspended in 135 μL Buffer T to which 15 μL of Dre mRNA (at 1 μg/μL ...
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bioRxiv - Cancer Biology 2022Quote: ... single-cell suspensions were depleted of lineage-committed hematopoietic cells using a Lineage Cell Depletion Kit according to manufacturer’s protocol (EasySep™, StemCell Technologies, Inc.). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... hiPSC were dissociated with accutase and seeded at a density of 35×103 cells/cm2 on Matrigel-coated dishes in mTeSR1 (STEMCELL Technologies) supplemented with 10 mM ROCKi Y-27632 10mM (Merck Millipore ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were isolated from PBMCs using an EasySep Human Monocyte Isolation Kit (all STEMCELL Technologies).
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bioRxiv - Cancer Biology 2022Quote: ... red blood cells were lysed using ammonium chloride (Stem Cell Technologies) and cells were washed and resuspended in PBS 2% FBS for counting ...
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bioRxiv - Bioengineering 2022Quote: ... and SCF (StemCell Technologies) in 90% RPMI + 10% heat inactivated FBS + 0.1 mM beta-mercaptoethanol (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were fed on any other day and passaged weekly using RelesR (catalog#05872, Stemcell Technologies, Vancouver, Canada) dissociation reagent in accordance to their manual ...
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bioRxiv - Neuroscience 2022Quote: ... hiPSCs dissociated with collagenase IV (Stem Cell Technologies) were cultured in suspension to form embryoid bodies (EB ...
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bioRxiv - Molecular Biology 2022Quote: ... or CD3-CD28 ImmunoCult (StemCell Technologies). Aliquots of cell culture supernatants were taken at 7 days post challenge for measurement of cytokine secretion using Luminex multiplex assays as described below ...
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bioRxiv - Neuroscience 2022Quote: ... 10 ug/mL GDNF (Stem cell technologies # 78058), 1μg/mL laminin (Life technologies # 23017-015) ...