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Citations for Stemcell Technologies :
8301 - 8350 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Molecular Biology 2019Quote: ... in mTeSR1 medium (Stem cell Technologies) and cultured in a 37°C incubator with 20% O2 and 5% CO2 ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were routinely passaged as clumps using gentle Cell Dissociation Reagent (Stemcell Technologies) approximately every 3-4 days ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were detached using Accutase (Stemcell Technologies) and plated on mTeSR1 supplemented with 10 µM of ROCK inhibitor (Y-27632 ...
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bioRxiv - Developmental Biology 2019Quote: ... Organoids were cultured in IntestiCult medium (#06005, Stemcell technologies) supplemented with Primocin antibiotic (1:50 ...
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bioRxiv - Immunology 2019Quote: Monocytes were enriched from the same leukopoiesis products as T-cells using RosetteSep kits (Stemcell Technologies), and were then cultured at 1-2×106/ml in 12 well-plates with differentiation media containing 50 ng/ml Interleukin 4 (IL-4 ...
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bioRxiv - Developmental Biology 2019Quote: ... Single ES cells were seeded onto aggrewells in a 24 well plate (Stem Cell Technologies, Cat. No. 34411) at 1.5 × 10^6 cells per well in EB-medium ...
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bioRxiv - Immunology 2019Quote: OT1 and OTII T cells were isolated from LN of TCR transgenic mice using either a CD8 or CD4 StemSep enrichment kit (STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2019Quote: ... B cells were purified using the EasySep negative selection mouse B cell enrichment kit (Stem Cell Technologies). B cell purity was assessed by flow cytometry using anti CD19 or B220 antibodies (we routinely observed over 98% purity) ...
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bioRxiv - Developmental Biology 2019Quote: ... and plated as a single cell suspension in mTeSR1 medium (STEMCELL Technologies) containing 10 μM Y27632 (Wako ...
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bioRxiv - Developmental Biology 2019Quote: ... mesoderm and endoderm lineages using STEMdiff™ Trilineage Differentiation Kit (STEMCELL Technologies). hPSCs reaching 70–80% confluency were harvested with TrypLE™ Select Enzyme (1X ...
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bioRxiv - Bioengineering 2019Quote: ... Homogeneous and reproducible EBs were generated by using 24-well plate AggreWell™ 800 (Stem Cell Technologies, catalog 34815). In each well ...
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bioRxiv - Bioengineering 2019Quote: Brain organoids were generated using the STEMdiff™ Cerebral Organoid Kit (Stem Cell Technologies) using the manufacturer protocol with some modifications [1,8,11,13] ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (Stem Cell Technologies). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Immunology 2019Quote: ... and Mononuclear cells were incubated with 1 ml of erythrocytes and the RossetteSep™ CD8+T cell enrichment cocktail to obtain Total CD8+T cells (15063; StemCell Technologies, Canada). The memory cells were eliminated using magnetic beads (8803 ...
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bioRxiv - Immunology 2019Quote: ... RosetteSep™ cocktail (15028, StemCell Technology, Vancouver, Canada) was added (50 µl/ml of blood ...
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bioRxiv - Clinical Trials 2019Quote: ... tumors were digested in collagenase IV (STEMCELL Technologies) for 1 hour at 37°C before being mechanically dissociated via passage through a 40 µM filter ...
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bioRxiv - Cell Biology 2019Quote: ... Hematopoietic stem cells were enriched using the EasySep Mouse Hematopoietic Progenitor Cell Enrichment kit (Stemcell Technologies) and 1*106 HSCs were administered via tail vein injection to recipient mice ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were passaged using dispase (STEMCELL Technologies) every 3 days ...
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bioRxiv - Developmental Biology 2019Quote: ES017 hESCs were grown in mTeSR1 (STEMCELL Technologies) in tissue culture dishes coated with Matrigel (Corning ...
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bioRxiv - Immunology 2019Quote: ... An ImmunoCult human CD3/CD28 T-cell activator (STEMCELL Technologies; cat. no. 10971) was used to activate positive control T cells according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... LV-CAR T cells were expanded by stimulation with an antibody cocktail (anti-CD3/CD28; STEMCELL Technologies; cat. no. 10971) combined with IL-2 (50 U/ml ...
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bioRxiv - Genetics 2019Quote: ... in mTeSR1 medium (STEMCELL Technologies). The direct differentiation of iPSCs into dopaminergic neurons was conducted as described before (49 ...
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bioRxiv - Microbiology 2019Quote: Human monocytes were negatively selected from buffy coats using the RosetteSep Monocyte Enrichment Kit (1mL/10mL buffy coat; StemCell Technologies) and differentiated into moDC ...
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bioRxiv - Neuroscience 2019Quote: ... and on day 12 Neural Rosette Selection reagent (STEMCELL Technologies) was used to select neural progenitor cells (NPCs ...
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bioRxiv - Neuroscience 2019Quote: ... in mTeSR1 (STEMCELL Technologies). cExN and cIN differentiation of iPSCs was performed using previously described protocols (33) ...
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bioRxiv - Cancer Biology 2019Quote: ... ALDH activity in the cells was measured by flow cytometry using the ALDEFLUOR kit (Stemcell technologies, Vancouver, BC, Canada). Cells with low and high levels of ALDH enzymatic activity (respectively ALDH- and ALDH+ cells ...
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bioRxiv - Genomics 2019Quote: ... supplemented with 12 µM CHIR-99021 (STEMCELL Technologies). Approximately 60 hours later ...
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bioRxiv - Genomics 2019Quote: ... iPSCs were dissociated with 1:3 Accutase and plated onto 24-well plates pre-coated with 0.5% GelTrex in mTeSR1 supplemented with 10uM Y-27632 ROCK Inhibitor (STEMCELL Technologies). 24 hours later another layer of GelTrex at 1.5% was added in mTeSR1 media ...
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bioRxiv - Genomics 2019Quote: ... They were passaged using 1:3 Accutase (STEMCELL Technologies).
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bioRxiv - Genomics 2019Quote: ... in Modified Tenneille’s Special Recipe 1 (mTeSR1, STEMCELL Technologies), supplemented with 1% Penicillin/Streptomycin (Gibco ...
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bioRxiv - Pathology 2019Quote: Peripheral blood samples were processed using a Ficoll-Paque PLUS gradient in a SepMateTM (Stem Cell Technologies) and frozen in 10% DMSO (Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated through Lymphoprep density gradient separation (Stemcell Technologies). Cells were used after cryopreservation.
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bioRxiv - Developmental Biology 2019Quote: H9 cells (WiCell, SLA# 16-W0026) were cultured in mTESR1 (Stem Cell Technologies). Cultured cells were replenished with fresh media every day ...
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bioRxiv - Immunology 2019Quote: ... Cells were then permeabilized with 0.3% Triton-X for 15 min followed by 30 min block in 0.1% Triton-X + 5% normal rat serum (Stem Cell Technologies). Cells were incubated in Drp1 p616 Ab overnight at 4°C in 0.1% Triton-X + 1% BSA and then in secondary antibodies (AF488 Goat anti-Rabbit) ...
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bioRxiv - Neuroscience 2019Quote: ... primitive neuroepithelia structures were formed and neural rosettes were selected with STEMDiff Neural Rosette Selection reagent (Stem Cell Technologies) and re-culture in suspension in NIM plus B27 supplement (Thermo Scientific ...
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bioRxiv - Cancer Biology 2019Quote: Fresh or culture progeny from total BM were counted and 5000 cells/well of a 24-well plate were resuspended in Methocult (M3434, Stem Cell Technologies, Vancouver, BC, Canada). Colonies were counted at day 7 ...
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bioRxiv - Immunology 2019Quote: ... and then purified with EasySep Human Neutrophil Kit (STEMCELL Technologies Inc. Vancouver, Canada), following manufacturers protocol ...
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bioRxiv - Immunology 2019Quote: ... neutrophils were isolated using a density gradient with HetaSep (STEMCELL Technologies Inc. Vancouver, Canada) and then purified with EasySep Human Neutrophil Kit (STEMCELL Technologies Inc ...
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bioRxiv - Immunology 2019Quote: ... according to the culture and differentiation protocols from Stemcell Technologies (Stemcell Technologies Inc. Cambridge, MA). A megakaryoblastic cell line (Meg-01 ...
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bioRxiv - Immunology 2019Quote: ... according to the culture and differentiation protocols from Stemcell Technologies (Stemcell Technologies Inc ...
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bioRxiv - Immunology 2019Quote: Cord blood CD34+ hematopoietic stem cells were purchased and cultured in StemSpan II media with the MK supplemental cytokines (StemSpan II, Stemcell Technologies, Inc.), according to the culture and differentiation protocols from Stemcell Technologies (Stemcell Technologies Inc ...
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bioRxiv - Neuroscience 2019Quote: ... and mTeSR1 medium (Stem Cell Technology) and up to three clones per individual were validated ...
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bioRxiv - Immunology 2019Quote: ... T cells were extracted from spleen and CD4+ T cells were then purified using EasySep immunomagnetic negative selection (Stem Cell Technologies). Cells were then activated on plates as mentioned ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were purified using negative enrichment kits (Stem Cell Technologies).
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bioRxiv - Immunology 2019Quote: ... cells were separated with Lymphoprep (Stem Cell Technologies), following the manufacturer’s instructions and stained for flow cytometry ...
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bioRxiv - Immunology 2019Quote: Total CD8+ T cells were isolated from the peripheral blood of 2 healthy donors using EasySep™ Human CD8+ T Cell Enrichment Kit (StemCell Technologies). Isolated CD8+T cells were activated with Dynabeads™ Human T-Activator CD3/CD28 beads (Invitrogen ...
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bioRxiv - Biophysics 2019Quote: ... and SM1 (NeuroCult, Stem Cell Technologies). Mixed neuron and astrocyte cell suspension was plated at a concentration of 200,000 cells/mL onto glass bottom 35 mm petri dishes (CellVis ...
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bioRxiv - Biophysics 2019Quote: ... Cells were washed and resuspended in phenol red-free Neurobasal Medium (Lifetech) supplemented with L-glutamine (Stem Cell Technologies) and SM1 (NeuroCult ...
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bioRxiv - Neuroscience 2019Quote: ... The supernatant was subsequently plated in a 96-well V-bottom at a density of 20,000 cells with Neural Induction Medium (StemCell Technologies) supplemented with dual SMAD inhibitors SB321542 (10 μM ...