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Citations for Stemcell Technologies :
8251 - 8300 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... at a density of 9000 cells per well in 100 μl of Embryoid Body Formation Medium (STEMdiff Cerebral organoid Kit, STEMCELL Technologies) with either addition of 10 μM Y-27632 (Tocris ...
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bioRxiv - Cell Biology 2019Quote: ... Purified Collagen 1 (STEMCELL Technologies Inc), diluted in 1:5 DBPS diluted complete medium ...
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bioRxiv - Cell Biology 2019Quote: ... In either situation cells were resuspended in APEL 2 medium (Stem Cell Technologies) containing 10µM ROCKi ...
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bioRxiv - Cell Biology 2019Quote: ... For this we used a 50/50 mix between standard KOSR medium and mTESR medium (Stem Cell Technologies) and the addition of 20µM Synthechol (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: ... Primary CD4+ T cells were purified from the blood of healthy donors by negative selection using the Human CD4+ T Cell Enrichment cocktail (StemCell Technologies). These primary cells were cultured in complete RPMI 1640 medium.
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bioRxiv - Microbiology 2019Quote: ... or the EasySep Human CD4+ T Cell Isolation Kit (STEMCELL Technologies, Vancouver, BC, Canada, Cat. #17952) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... were purchased from STEMCELL Technologies and cultured in X-Vivo 15 media (Lonza).
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bioRxiv - Microbiology 2019Quote: ... or RosetteSep Human B cell enrichment cocktail (STEMCELL Technologies) and then isolated with Ficoll-Paque Plus (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... gently layered onto room temperature Ficoll (Biosciences AB, Uppsala, Sweden Cat #:17-1440-03 Lot #: 10253776) in Sepmate-50 tubes (STEMCELL Technologies, Vancouver ...
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bioRxiv - Neuroscience 2019Quote: ... cells were rinsed with phosphate-buffered saline (PBS) (5 ml) and detached by addition of ‘Gentle Dissociation Reagent’ (3 ml) (STEMCELL Technologies; Cat. No. 07174) stored at room temperature ...
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bioRxiv - Neuroscience 2019Quote: Human iPSCs at low passage number were cultured in mTeSR medium (STEMCELL Technologies; Vancouver, BC, Canada; Cat. No. 85850) in 10-cm culture dishes (Thermo-Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... were seeded to Matigel coated 12-well plate at a density of 70-80% confluency in mTeSR plus medium (STEMCELL technology) with 10 μM of Y-23632 (Rock inhibitor ...
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bioRxiv - Genomics 2019Quote: CD4+ T cells were enriched from PBMCs by negative MACS selection using the EasySep™ Human CD4+ T Cell Isolation Kit (STEMCELL Technologies SARL, Grenoble, France), according to manufacturer’s instructions.
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bioRxiv - Genomics 2019Quote: ... and passaged at 80-85 % confluency using Gentle Cell Dissociation Reagent (StemCell Technologies). For evaluation of pluripotency by flow cytometry ...
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bioRxiv - Genomics 2019Quote: ... and 1 mg/mL heparin (StemCell Technologies) and replaced every other day ...
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bioRxiv - Genomics 2019Quote: ... coated T25 flasks in mTeSR1 medium (StemCell Technologies, Vancouver, Canada) and passaged at 80-85 % confluency using Gentle Cell Dissociation Reagent (StemCell Technologies) ...
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bioRxiv - Genomics 2019Quote: ... and a negative surface marker SSEA1(StemCell Technologies, 60060PE). Cells were washed with DPBS followed by data acquisition on a SONY SH800 flow cytometer.
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bioRxiv - Genomics 2019Quote: ... and for 1 h at 4 0C with a combination of conjugated antibodies to surface proteins TRA-1-60 (StemCell Technologies, 60064PE), TRA-1-81(StemCell Technologies ...
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bioRxiv - Genomics 2019Quote: ... the medium was changed to STEMdiff APEL2 medium (StemCell Technologies) containing 10 μM CHIR99021 (Tocris 4423) ...
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bioRxiv - Genomics 2019Quote: ... TRA-1-81(StemCell Technologies, 60065AZ), key transcription factors OCT3/4 (SantaCruz Biotechnology ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 ng/mL Recombinant Human Epidermal Growth Factor (EGF) (StemCell Technologies, Cat 02633), 10 ng/mL Recombinant Human Basic Fibroblast Growth Factor (Bfgf ...
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bioRxiv - Cancer Biology 2019Quote: ... 10 ng/mL Recombinant Human Basic Fibroblast Growth Factor (Bfgf) (StemCell Technologies, Cat 02634), 2 μg/mL Heparin (StemCell Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... 2 μg/mL Heparin (StemCell Technologies, Cat 07980), 1× antibiotic-antimicotic (Life Technologies Gibco ...
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bioRxiv - Immunology 2019Quote: ... antibody and negative selection was performed by using an EasySep Biotin Selection Kit (Stemcell Technologies) following manufacturers instructions ...
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bioRxiv - Physiology 2019Quote: Monocytes were isolated by the University of Pennsylvania Human Immunology Core from healthy apheresis donors with institutional review board approval by RosetteSep™ human monocyte enrichment cocktail (Stem Cell Technologies, Vancouver, Canada), and MΦs were differentiated by 12-days adherence culture in RPMI 1640 media containing 10% human serum and 1x cell culture pen/strep antibiotic mix (Gibco) ...
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bioRxiv - Molecular Biology 2019Quote: ... CD4+ T-cells were isolated using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies Inc. ...
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bioRxiv - Physiology 2019Quote: ... Single cell suspensions were seeded on plates coated with 10µg/ml Vitronectin XF™ (STEMCELL Technologies) in maintenance media supplemented with 10µM ROCK Inhibitor Y-27632 (Selleckchem ...
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bioRxiv - Developmental Biology 2019Quote: ... gamma irradiated MEF feeder cells (Stem Cell Technology, #00323). Two days after nucleofection ...
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bioRxiv - Synthetic Biology 2019Quote: T cells were purified from normal donors by negative selection using the RosetteSep Human CD4+ T Cell Enrichment Cocktails according to the manufacturer’s protocols (StemCell Technologies). Primary cells were activated and expanded using Human T-activator CD3/CD28 Dynabeads (Cat # 11161D ...
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bioRxiv - Cancer Biology 2019Quote: ... STO-609 (#73862) was purchased from STEMCELL Technologies (Vancouver, BC, Canada).
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bioRxiv - Immunology 2019Quote: ... Pure cell populations were isolated using EasySep Human negative selection kits (STEM CELL technologies, >95% purity). In addition ...
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bioRxiv - Cancer Biology 2019Quote: ... the resulting cells were cultured in NeuroCult medium (StemCell Technology, Vancouver, BC, Canada) supplemented with 0.2% heparin (StemCell Technology) ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 0.2% heparin (StemCell Technology), hEGF (20 ng/mL ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were maintained in feeder free conditions, batch fed daily with Essential 8(Chen et al., 2011) or mTeSR⅄1 (STEMCELL Technologies, 85850) cell culture media ...
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bioRxiv - Cell Biology 2019Quote: ... Passaging of cells was performed by clump dissociation using ReLeSR (STEMCELL technologies, 05873) following manufacturer guidelines.
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bioRxiv - Neuroscience 2019Quote: ... with mTeSR1 media (Stem Cell Technologies) and passaged weekly using 1mM EDTA or Accutase (Sigma) ...
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bioRxiv - Developmental Biology 2019Quote: ... and iPSCs were maintained in feeder-free conditions with mTeSR1 (STEMCELL Technologies); see Supplemental Information ...
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bioRxiv - Developmental Biology 2019Quote: ... CMs were differentiated using STEMdiff Cardiomyocyte differentiation kits (STEMCELL Technologies). CM Calcium imaging was captured by loading cells with Fluo-4 dye and taking images at 4 Hz for 30 seconds ...
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bioRxiv - Microbiology 2019Quote: Peripheral blood mononuclear cells (PBMCs) were processed from Leukopak (STEMCELL Technologies, Cat. No. 70500) using the recommended protocol for cell isolation without density centrifugation ...
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bioRxiv - Developmental Biology 2019Quote: ... The lines were grown and expanded in feeder-free conditions using mTeSR1 medium (STEMCELL Technologies), at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2019Quote: HSPCs were isolated using Easy Sep Mouse Hematopoietic Progenitor Cell Isolation Kit (StemCell Technologies) according to manufactures’s protocol ...
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bioRxiv - Immunology 2019Quote: Isolated HSPCs from Apoe−/−Il27ra+/− and Apoe−/−Il27ra−/− mice infused with Ang II were sorted and plated at 1×103 cells in 1ml of MethoCult® GF M3434 (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... in mTeSR1 medium (StemCell Technologies) with daily medium change ...
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bioRxiv - Biochemistry 2019Quote: ... collected by 5 min centrifugation at 300 × g and resuspended in mTeSR1 supplemented with 10 µM Rock inhibitor Y-27632(2HCl) (72304, StemCell Technologies) for the first day.
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bioRxiv - Biochemistry 2019Quote: ... Embryoid bodies (EBs) were generated from feeder-free iPSCs by incubating colonies with Collagenase Type IV (7909, StemCell Technologies) for 10 min ...
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bioRxiv - Biochemistry 2019Quote: ... Resulting NPCs were passaged using Accutase (StemCell Technologies) and split at a maximum ratio of 1:4 ...
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bioRxiv - Cancer Biology 2019Quote: ... cultured and expanded in Myelocult™ medium (Stemcell Technologies) supplemented with 1% penicillin-streptomycin (Life technologies) ...
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bioRxiv - Immunology 2019Quote: ... and the EasySep Biotin Positive Selection Kit (Stemcell Technologies) according to product instructions ...
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bioRxiv - Neuroscience 2019Quote: ... ImmunoCult Human CD3/CD28 T cell Activator and 20ng rhIL2 (Stemcell Technologies) was added to culture medium ...
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bioRxiv - Neuroscience 2019Quote: ... anti-human CD40 antibody (clone 5C3) and 20 ng rhIL4 (Stemcell Technologies) was added to culture medium ...