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Citations for Stemcell Technologies :
8151 - 8200 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: EasySep separation kits (StemCell Technologies, T Cells: #19661, Neutrophils: #19257) were used to isolate CD3+ T cells or neutrophils directly from human whole blood ...
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bioRxiv - Immunology 2020Quote: ... Following enumeration using 3% acetic acid with methylene blue (StemCell Technologies), splenocytes were resuspended in phosphate-buffered saline and forty to sixty million cells were injected either intraperitoneally or intravenously (via retro-orbital injection under isoflurane anesthesia ...
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bioRxiv - Immunology 2020Quote: Specific cell types were isolated from the PBMC fraction using the following kits (STEMCELL Technologies Inc.) per manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: Whole blood was collected into EDTA tubes and then diluted with an equal volume of phosphate-buffered saline (PBS) + 2% fetal bovine serum (FBS) and layered over Ficoll using SepMate™-50 tubes (STEMCELL Technologies Inc., Vancouver, Canada). Cells were centrifuged at 1200×g for 10 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... and SepMateTM 50 mL tubes (STEMCELL Technologies) (Grievink et al. ...
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bioRxiv - Immunology 2020Quote: ... and either the Big Easy or Easy eight magnets (StemCell technologies) using protocols from the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... T lymphocytes were purified by negative-selection using Rosette tetramers (Stemcell Technologies Inc) and the purity was monitored by flow cytometry ...
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bioRxiv - Immunology 2020Quote: ... Using a CD4+ T cell enrichment kit (StemCell Technologies, USA), CD4+ T cells were isolated from bulk splenocytes by negative selection ...
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bioRxiv - Immunology 2020Quote: ... respectively (STEMCELL technologies), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Preliminary experiments were also performed after isolation of monocytes using the EasySep human monocyte enrichment kit with CD16 depletion (StemCell Technologies) and the EasySep human monocyte isolation kit (StemCell Technologies ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were isolated from PBMCs by negative magnetic selection using the EasySep human monocyte enrichment kit without CD16 depletion (StemCell) following the manufacturer’s instructions and using a Big Easy magnet or Easy 50 magnet (StemCell Technologies, Vancouver, Canada). Enriched monocytes were generally >80% CD14-positive ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated with gradient centrifugation using Lymphoprep (StemCell Technologies) and the red blood cells were removed by inclubating the cells with RBC lysis buffer (Tonbo Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... and the EasySep human monocyte isolation kit (StemCell Technologies) with equivalent results ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from de-identified human leukapheresis-processed blood (New York Biologics, Inc) by centrifugation through Lymphoprep (Stem Cell Technologies.) density gradient ...
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bioRxiv - Immunology 2020Quote: ... infection efficiency was determined by GFP expression and infected cells were isolated using RoboSep mouse CD8a positive selection kit II (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2020Quote: EasySep™ Direct Human NK cell Isolation Kit (StemCell Technologies);
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bioRxiv - Immunology 2020Quote: ... And pNK cells were isolated from whole blood by negative selection using the EasySep™ Direct Human NK cell Isolation Kit (StemCell Technologies). Following isolation ...
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bioRxiv - Immunology 2020Quote: ... CD4+ or CD3+ cells were enriched from leukopheresis products through negative selection (RosetteSep, STEMCELL Technologies) before density gradient centrifugation ...
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bioRxiv - Neuroscience 2020Quote: ... plates in mTeSR1 medium (Stemcell Technologies). Lack of genomic integration of Sendai virus into iPSCs was confirmed by PCR and karyotype analysis confirmed the expected chromosomal copy numbers 15 ...
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bioRxiv - Immunology 2020Quote: Human cord-blood CD34+ cells were isolated by magnetic sorting using EasySep human CD34 positive selection kit (Stem Cell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Cells were cryopreserved in Cryostor CS10 (Stemcell Technologies) in aliquots of 200,000 cells each ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were resuspended in SFEM II (StemCell Technologies) with 5 μg/mL purified anti-CD28 (clone CD28.2 ...
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bioRxiv - Immunology 2020Quote: ... CD34+ cells were first thawed and rested for 48 h in SFEM II (StemCell Technologies) with 75 nM StemRegenin 1 (StemCell Technologies) ...
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bioRxiv - Immunology 2020Quote: ... CD4T cells were negatively isolated from the digested spleen and LNs of indicated donor mice with Mouse CD4T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) according to the manufacturer’s protocol and labeled with 1.0 μM CFSE (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... T-cells were obtained from PBMCs using the EasySep Human T-cell Isolation Kit (Stemcell Technologies). MLRs were set up in 96 well plates ...
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bioRxiv - Immunology 2020Quote: ... and removed following separation by using an EasySep™ magnet (STEMCELL technology, Canada) according to manufacturer’s instructions [28] ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were isolated using the EasySep Human Mono Isolation Kit (STEMCELL technology, Canada) [28] ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T-cells were isolated using a RosetteSep(tm) kit (Stemcell Technologies, Vancouver, BC) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T-cells were isolated from the pooled cell suspensions using an EasySep(tm) Mouse CD4+ T Cell Isolation Kit from Stemcell Technologies (Vancouver, Canada) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Total CD4 T cells from donor mice were purified from spleens using STEMCELL EasySep CD4 T Cell Isolation Kit according to manufacturer’s instruction (STEMCELL Technologies). Depending on individual experiment ...
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bioRxiv - Cell Biology 2020Quote: NSC and GSC lines were grown in NeuroCult NS-A basal medium (StemCell Technologies) supplemented with B27 (Thermo Fisher) ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated with EasySep Human CD4 T Cell Isolation Kit (STEMCELL Technologies, 17952).
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were purified from non-adherent cells using Human Resting CD4+ T cell Isolation Kit (STEMCELL Technologies, 17962). A specific reagent to isolate resting T cells was selected to avoid the presence of pre-activated CD4+ T cells in sequencing samples ...
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bioRxiv - Immunology 2020Quote: ... we treated CD4+ T cells with either αCD3αCD28 (ImmunoCult™ Human CD3/CD28 T Cell Activator; STEMCELL Technologies, 10971) or IFN I (1:1000 ...
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bioRxiv - Immunology 2020Quote: Cells were isolated using a human negative selection-based NK isolation kit (RosetteSep, STEMCELL Technologies). Purified NK cells were then cultured in stem cell serum-free growth medium (CellGenix GMP SCGM ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated using the CD4+ Negative Selection Kit (StemCell Technologies, Cat# 19752) followed by fluorescence-activated cell sorting ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were obtained by negative selection using the EasySep Human CD4+ Enrichment kit (Stem Cell Technologies), and stimulated with anti-CD3-coated magnetic beads (Dynabeads M-450 tosyl-activated ...
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bioRxiv - Immunology 2020Quote: ... B cells were enriched by negative selection using EasySep mouse B cell enrichment kit as per manufacturer’s instructions (StemCell Technologies Inc., Tukwila, WA, USA). Enriched B cells were then resuspended in FACS buffer and stained and sorted as described previously(Carbonetti et al. ...
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bioRxiv - Immunology 2020Quote: ... 3.5 nM UM171 (StemCell Technologies), 40 ng/mL SCF ...
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bioRxiv - Immunology 2020Quote: Purified CD34+ bone marrow cells from healthy individuals were either purchased directly from StemCell Technologies or isolated from fresh human bone marrow ...
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bioRxiv - Immunology 2020Quote: ... with 75 nM StemRegenin 1 (StemCell Technologies), 3.5 nM UM171 (StemCell Technologies) ...
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bioRxiv - Immunology 2020Quote: ... Isolated CD3+ cells and tumor-infiltrating lymphocytes (TILs) were maintained in Human T Cells Expansion (STEMCELL Technologies, Vancouver, Canada) medium supplemented with 5% of heat-inactivated human AB serum (Omega Scientific ...
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bioRxiv - Immunology 2020Quote: ... naive CD4+ T cells were isolated using a RoboSep CD4+ Naïve T cell negative selection kit (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2020Quote: ... Cells were cultured in BrainPhysTM medium (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2020Quote: Primary CD4+ T-cells for in-vitro HIV-1 infection were isolated from total blood of healthy individuals using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2020Quote: ... Memory human CD8+ T cells were purified from healthy donor blood samples using the EasySep Human Memory CD8+ T cell enrichment kit (StemCell Technologies), cells were routinely ∼90% CD8+RO+RA-.
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bioRxiv - Immunology 2020Quote: Total human CD8+ T cells were purified from healthy donor blood samples using the EasySep Negative human CD8+ T cell isolation kit (StemCell Technologies). CD8+ T cells were routinely ∼90% pure ...
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bioRxiv - Immunology 2020Quote: ... Total mDC and pDC were negatively enriched from PBMCs using the EasySep™ Human Pan-DC Pre-Enrichment Kit (StemCell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Total T-cells were purified from the same donor’s PBMC using the EasySep™ Human T cell Isolation kit (StemCell Technologies). Purity of total CD4+ and CD8+ T-cells was ∼94% (Supplementary Fig 2) ...
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bioRxiv - Immunology 2020Quote: ... prepared using the CD4 Positive Selection kit II (StemCell Technologies, Tullamarine, VIC, Australia), were used as antigen presenting cells ...