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Citations for Stemcell Technologies :
8501 - 8550 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: Cells in semi-solid media were prepared using the Neurocult NCFC Assay Kit (Stem Cell Technologies) per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... in mouse neural stem cell (mNSC) media consisting of NeuroCult Proliferation media (Stem Cell Technologies) supplemented with heparin ...
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bioRxiv - Immunology 2019Quote: ... Collagenase Type IV (STEMCELL Technologies, Catalog #07909); ROCKi ...
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bioRxiv - Cell Biology 2019Quote: ... with PneumaCult-Ex Medium (Stem Cell Technologies) supplemented with ROCK Inhibitor (5 μM ...
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bioRxiv - Cell Biology 2019Quote: ... and hydrocortisol solution (96 ng/ml; Stem Cell Technologies). Under these conditions ...
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bioRxiv - Neuroscience 2019Quote: ... a single retina (divided into 8 uniform pieces) was added to 500 uL Accumax (STEMCELL Technologies). Each retina was chemically digested for 70 mins with agitation on a nutator ...
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bioRxiv - Genomics 2019Quote: ... Undifferentiated hESCs were aggregated by preparing a single cell suspension in mTeSR media (STEMCELL Technologies) at 1×106 cells/mL and overnight culture in six-well ultra-low attachment plates (Costar ...
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bioRxiv - Genomics 2019Quote: ... were seeded onto Matrigel®-coated six-well plates at a density of 50,000 cells/cm2 and maintained in mTeSR1 media (StemCell Technologies) for 3-4 days with media changed daily ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 ng/ml human recombinant epidermal growth factor (StemCell Technologies), 1% Penicillin/Streptomycin (Gibco ...
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bioRxiv - Physiology 2019Quote: ... and cultured on plates coated with 10µg/ml Vitronectin XF™ (STEMCELL Technologies). Colonies were regularly passaged by short-term incubation with 0.5mM EDTA in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Then a warm solution containing 20 µM blebbistatin (final concentration in cell medium; Stemcell Technologies, 72402) or DMSO (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2019Quote: ... and cultured at 37°C (5% CO2) for 7 days in 300 µL StemSpan™ SFEM (#09650 Stemcell Technologies) supplemented with 100 ng/mL SCF (Peprotech ...
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bioRxiv - Bioengineering 2019Quote: ... Initial HSPC isolation was performed using defined protocols of EasySep™ Mouse Hematopoietic Progenitor Cell Enrichment Kit and Magnet (#19756, #18000, Stemcell Technologies, CA). A secondary isolation further enriched the HSPC population by collecting the Lin− Sca-1+ c-kit+ (LSK ...
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bioRxiv - Bioengineering 2019Quote: ... MSCs were isolated from bone following commercially available defined protocols of MesenCult™ Proliferation Kit with MesenPure™ (#05512 Stemcell Technologies, CA). The MSCs were cultured in one T25 flask per mouse and allowed to expand for two weeks prior to collection (zero passage) ...
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bioRxiv - Microbiology 2019Quote: ... freshly isolated PBMCs obtained from healthy donor peripheral blood (lymphocyte separation media; StemCell Technologies) were subjected to CD14+ magnetic bead positive selection using the MojoSort Human CD14 Selection Kit (BioLegend) ...
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CUX1 and IκBζ mediate the synergistic inflammatory response to TNF and IL-17A in stromal fibroblastsbioRxiv - Immunology 2019Quote: ... Neutrophils (PMNs) purified from blood using the neutrophil isolation kit (Stem Cell Technologies) and peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Cancer Biology 2019Quote: ... Low density CD3-CD19-CD11b-cells simultaneously depleted of neutrophils and red cells were isolated on Lymphoprep using RosetteSep™ and the >90% pure CD34+ cells isolated using EasySep (both STEMCELL Technologies). Cells were stored frozen at −176 °C in DMSO with 90% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2019Quote: ... Prior to use for transplanting mice the cells were thawed in Iscove’s modified Dul-becco medium with 10% FBS (both STEMCELL Technologies) and 10 mg/mL DNase I (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2019Quote: ... and re-suspended in Hanks balanced salt solution (STEMCELL Technologies) with 2% FBS.
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bioRxiv - Cancer Biology 2019Quote: Red blood cell lysis was first performed using Ammonium Chloride solution from STEMCELL Technologies for 10 minutes on ice ...
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bioRxiv - Genomics 2019Quote: ... ES cells were grown on 0.1% gelatincoated culture dishes (Stem Cell Technologies, Vancouver, BC, Canada).
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bioRxiv - Genetics 2019Quote: ... The electroporated cells were resuspended with Stemspan II media with CC100 cytokine cocktail (Stem Cell Technologies). Two guides targeting GFI1B coding regions (sgRNA1 ...
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bioRxiv - Genetics 2019Quote: Umbilical cord blood Lineage negative (Lin-) and CD34+ cells were obtained using StemSep system according to the manufacturer’s protocol (Stem Cell Technologies, Canada). Lin-CD34+ cells were sorted to obtain HSC (CD34+38-/lowCD45RA-CD90+) ...
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bioRxiv - Genetics 2019Quote: ... 500 CD34+ HSPCs were plated in 1ml methylcellulose media (# H4034, Stem Cell Technologies). Primary CFU-C colonies were counted after 14 days ...
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bioRxiv - Genetics 2019Quote: ... in MethoCult-M3434 semisolid culture medium (STEMCELL TECHNOLOGIES). Colonies were scored on day 3 for erythroid colony-forming units (CFU-Es ...
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bioRxiv - Genetics 2019Quote: ... 5×104/mL bone marrow and 5×105/mL spleen cells were cultured with MegaCult-C Kit (STEMCELL TECHNOLOGIES) for 9 days ...
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bioRxiv - Immunology 2019Quote: ... ImmunocultTM-XF T cell expansion medium (STEM CELL Technologies; Vancouver, Canada). Control comparisons were performed using RPMI 1640 medium with L-glutamine and phenol red (Thermo Fisher ...
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bioRxiv - Cancer Biology 2019Quote: ... Tumors were mechanically dissociated and digested for 1 h with 1x collagenase/hyaluronidase (10X Solution, Stem Cell Technology, Cambridge, MA) containing DNase I (2 U/ml ...
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bioRxiv - Genomics 2019Quote: ... CD34+ HSPCs were incubated in StemSpan SFEM II medium with StemSpan Myeloid Expansion Supplement (Stem cell technologies, Vancouver, Canada) for 10 days ...
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bioRxiv - Genomics 2019Quote: Mouse bone marrow cells (2.5×104) suspended in methylcellulose semisolid medium (Methocult M3231) (Stem Cell Technologies) were plated in 35-mm culture dishes in the presence of 0.5 % FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×105 cells were seeded in 35mm dishes in duplicate in H4100 base methocult (StemCell Technologies, Inc.), supplemented with 10% FBS and the recommended volume of IMDM in the presence increasing concentrations of rigosertib ...
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bioRxiv - Cancer Biology 2019Quote: ... G797 cells were maintained in serum-free NeuroCult™ NS-A Basal Medium (STEMCELL Technologies Canada Inc) supplemented with N2 ...
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bioRxiv - Neuroscience 2019Quote: ... The hPSCs were passaged approximately once per week with ReLeSR media (STEMCELL Technologies). All hPSC studies were approved by the Embryonic Stem Cell Research Oversight (ESCRO ...
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bioRxiv - Neuroscience 2019Quote: ... in mTeSR1 media (STEMCELL Technologies). Human PSCs from passage number 15 to 30 were used ...
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bioRxiv - Immunology 2019Quote: ... PBMCs were then isolated using Lymphoprep (STEMCELL Technologies, cat. nr. 07801) according to protocol and used in subsequent experiment (lymphocyte population analysis ...
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bioRxiv - Immunology 2019Quote: for CyTOF and cytokine analysis: Venous blood was obtained by venipuncture and prepared for peripheral blood mononuclear cells (PBMCs) using the SepMate system (STEMCELL Technologies) which were cryopreserved until used for the following studies.
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bioRxiv - Neuroscience 2019Quote: Neurons were differentiated from the WA09 (H9) pluripotent stem cell line maintained in mTesr1 medium (Stem Cell Technologies, Vancouver, BC, Canada). Stem cells were plated in 6-well plates coated in Matrigel in feeder-free conditions with daily media changes and split 1:6 one day before reaching confluency to ensure maintenance of pluripotency ...
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bioRxiv - Microbiology 2019Quote: ... coated plates and cultured in mTESR1 medium (Stem Cell Technologies) for 3 days with daily medium changes ...
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bioRxiv - Cancer Biology 2019Quote: ALDH1 enzymatic activity was determined using the ALDEFLUOR kit (Stem Cell Technologies) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2019Quote: ... dispase (5 U/ml) (Stemcell Technologies), DNase I (2000 U/ml ...
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bioRxiv - Immunology 2019Quote: ... Total CD4+ T cells were isolated by negative selection using magnetic beads (StemCell Technologies), and incubated with Fixable Viability Dye eFluor 780 (eBioscience ...
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bioRxiv - Cancer Biology 2019Quote: ... We then collected the cells and plated them at the same concentration (using RPMI) into cytokine enriched methylcellulose media for colony formation (MethoCultTMGF H4434, StemCell Technologies, Vancouver, CA). After 7 days at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: Patient-derived PB and/or BM MNC were plated at 4 x 104 cells per well in 96-well plates in 200 µL of RPMI supplemented with 10% fetal bovine serum and 10X StemSpan (StemCell Technologies #02691) (Pardanani et al. ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were maintained in RPMI supplemented with StemSpan (StemCell Technologies #02691) for 72 hours before experiments were conducted.
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bioRxiv - Immunology 2019Quote: ... using antibody-coated magnetic beads with a RoboSep instrument (StemCell Technologies). Purified CD4+ T-cells were stimulated for 3 days with concanavalin A (5μg/mL ...
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bioRxiv - Immunology 2019Quote: ... Tissue was digested in collagenase IV (STEMCELL Technologies) for 1 hour at 37 degrees Celsius and then mechanical dissociated via 40-uM filter ...
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bioRxiv - Immunology 2019Quote: ... Bulk T cells were isolated from PBMCs using Easysep Human T Cell Isolation Kit (STEMCELL Technologies) and activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors (Trima Apheresis, Blood Centers of the Pacific) by Ficoll centrifugation with SepMate tubes (STEMCELL Technologies). Bulk T cells were isolated from PBMCs using Easysep Human T Cell Isolation Kit (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2019Quote: ... coated tissue culture plates at ∼40,000 cells/cm2 in mTeSR1 (StemCell Technologies) until confluency reached 85-95% ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×107/ml of PBMCs were used for isolation of CD4+ CD127low CD25+ Regulatory T cells using the EasySep™ CD4+ CD127low CD25+ Human Regulatory T Cell Isolation Kit (STEMCELL Technologies, MA) following the manufacturer’s protocol.