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Citations for Stemcell Technologies :
7101 - 7150 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Colon organoids were harvested for immunoblotting by dissolving the Matrigel with 500 µl Gentle Cell Dissociation Reagent (Stemcell Technologies, UK) for 1 minute ...
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bioRxiv - Cell Biology 2020Quote: Colon organoids from K8flox/flox;Villin-Cre-ERT2 mice were isolated according to a modified previously described method (Stemcell Technologies, UK). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... domes diluted 1:1 with advanced DMEM/F12 media (Stemcell Technologies, UK), with 50 μl domes per well ...
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bioRxiv - Cell Biology 2020Quote: ... Cerebral organoids were generated and grown using the STEMdiff Cerebral Organoid Kit (Stem Cell Technologies, 08570) according to manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... The buffy coat was first separated by Ficoll-Paque Plus solution and the NK cells were then purified from the Peripheral Blood Mononuclear Cells (PBMCs) by negative selection using the EasySep Human NK Cell Enrichment Kit (STEMCELL Technologies), according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Naïve CD4 T cells were isolated from CB mononuclear cells (CBMCs) obtained using Lymphoprep (StemCell Technologies Inc., Canada), followed by a first step of negative selection by magnetic bead separation using EasySep Human Naïve CD4 T cell isolation kit (STEMCELL Technologies Inc. ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a first step of negative selection by magnetic bead separation using EasySep Human Naïve CD4 T cell isolation kit (STEMCELL Technologies Inc., Canada) and FACS on a BD FACSAriaTM II using the following markers/staining ...
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bioRxiv - Cell Biology 2020Quote: ... suspended in Hanks’ Balanced Salt Solution (HBSS; STEMCELL Technologies) supplemented with 2% FBS and 1.5ug/mL anti-human CD32 antibody (Clone IV.3 ...
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bioRxiv - Cell Biology 2020Quote: ... CD34+ cells were enriched by EasySep™ from the light-density fraction of Lymphoprep™ or RosetteSep™-depleted CD11b+;CD3+;CD19+ cells (STEMCELL Technologies) and then used after cryopreservation in DMSO and fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: Frozen CB cells were thawed by drop-wise addition to Iscove’s Modified Dulbecco’s Medium (IMDM) (STEMCELL Technologies) supplemented with 10% FBS and 10 µg/mL DNase (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... and then used after cryopreservation in DMSO and fetal bovine serum (FBS, STEMCELL Technologies). Three pools of cord bloods consisting of collections from 646 ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 2% FBS and 1.5ug/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies). Cells were stained for 20-30 minutes on ice with 1:200 anti-CD11b-BV711 (Clone M1/70 ...
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bioRxiv - Cell Biology 2020Quote: ... and 3 units/mL EPO (STEMCELL Technologies) in the presence of 1 µM GSK-J4 (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... The media was removed from the pellet and 200 ⎧l Accumax (STEMCELL technologies 07921) was added and incubated for 20 mins at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... and 50 ng/mL EGF (StemCell Technologies). The media was changed every 2-3 days ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were resuspended gently in 0.5 mL RPMI media before following the negative selection EasySep™ Mouse Neutrophil Enrichment Kit (StemCell Technologies). Neutrophils were labeled with 5 μM CellTracker (CFMDA ...
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bioRxiv - Cell Biology 2020Quote: ... plates at a concentration of 3×104 cells/cm2 in mTeSR1 medium (Stem Cell Technologies, Vancouver, CA) supplemented with 10μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Cell Biology 2020Quote: ... 200 μM/L sodium butyrate (NaB, Stem Cell Technologies) and 50 μg/ml ascorbic acid (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... medium was changed to mTeSR1 (Stemcell Technologies), with subsequent medium changes every second day ...
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bioRxiv - Cell Biology 2020Quote: ... cells were patterned into definitive endoderm using the StemDiff Definitive Endoderm kit (STEMCELL Technologies, #05110). On day 3 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were dissociated with Gentle Cell Dissociation Reagent (STEMCELL Technologies 07174 and passaged 1:4 (C1) or 1:6 (C2 ...
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bioRxiv - Developmental Biology 2020Quote: ... H9 ES cells (at 80-90% confluency) were enzymatic detached using dispase (1 mg/ml, Stemcell Technologies, 07923). Detached cells were transferred into medium at 3:1 ratio of mTeSR1 to Neural Induction Medium (NIM ...
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bioRxiv - Developmental Biology 2020Quote: ... coated dishes in mTeSR1 medium (Stemcell Technologies, 05850). Retinal organoids were generated based on a previously described protocol 30 with modifications ...
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bioRxiv - Developmental Biology 2020Quote: ... with SM1 (Stemcell Technologies, 05711) and N2 supplements (ThermoFisher ...
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bioRxiv - Developmental Biology 2020Quote: ... retinal organoid cells were cultured in RDM or BrainPhys neuronal medium (Stemcell Technologies, 05790) with SM1 (Stemcell Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... and 200 nM ascorbic acid (Stemcell Technologies,72132) till desired time ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 mM dibutyryl cyclic-AMP (Stemcell Technologies, 73882), and 200 nM ascorbic acid (Stemcell Technologies,72132 ...
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bioRxiv - Developmental Biology 2020Quote: ... 20 ng/ml GDNF (Stemcell Technologies, 78058), 1 mM dibutyryl cyclic-AMP (Stemcell Technologies ...
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bioRxiv - Genomics 2020Quote: ... The cells were passaged every 3–4 days using Accutase (Stemcell Technologies). The iPS cells conversion to EPS as previous reported47.
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bioRxiv - Genomics 2020Quote: Human Embryonic stem cells (H1 – WiCell, WA01, lot # WB35186) were cultured in mTeSR1 media (StemCell Technologies, 85850) under feeder-free conditions on Matrigel H1-hESC-qualified matrix (Corning ...
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bioRxiv - Genomics 2020Quote: ... with mTeSR1 (STEMCELL Technologies) at 37°C and 5% CO2 in a humidified atmosphere incubator ...
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bioRxiv - Genomics 2020Quote: ... H1 cells were daily fed with fresh mTeSR1 media and passaged every 4-5 days using ReLeSR reagent (StemCell Technologies, 05872). Cells were dissociated into single cells with TrypLE Express (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... The resuspended vesicles were layered onto a 6-18% Optiprep™ 11-step discontinuous velocity gradient (Stemcell Technologies, Vancouver, Canada), and centrifuged for 90 min at 176,000 × g in a SW40Ti rotor as previously described (48 ...
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bioRxiv - Developmental Biology 2020Quote: ... with 10µM ROCK inhibitor (StemCell Technologies, Y-27632). For screening of TBX5 exon 3 non-homologous end-joining (NHEJ ...
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bioRxiv - Developmental Biology 2020Quote: ... The culture was allowed to reach 80-90% confluency and induced with the Stemdiff Cardiomyocyte Differentiation Kit (Stemcell Technologies #05010), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were detached by Accutase Cell Detachment Solution (Stemcell Technologies #07920), diluted with 1X DPBS without Ca2+/Mg2+ and singularized using a P1000 filtered tip ...
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bioRxiv - Developmental Biology 2020Quote: ... and cultured in mTeSR-1 (StemCell Technologies Cat #05850) with 10µM ROCK inhibitor (StemCell Technologies ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Pluripotent human stem cells were grown in the absence of MEF for four passages in mTeSR1 (StemCell Technologies, 05850) and seeded on Geltrex (invitrogen ...
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bioRxiv - Genomics 2020Quote: ... 1 µM PD0325901 (Stemcell Technologies), 0.033% BSA solution (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... 3 µM CHIR99021 (Stemcell Technologies), 1 µM PD0325901 (Stemcell Technologies) ...
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bioRxiv - Genomics 2020Quote: ... with 2 mM L-Glutamine (Stemcell Technologies), 1x 2-Mercaptoethanol (Millipore) ...
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bioRxiv - Genomics 2020Quote: ... 1x NEAA (Stemcell Technologies), 3 µM CHIR99021 (Stemcell Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 10 μM Y-27632 (Stem Cell Technologies, Vancouver, Canada). The human iPSC line used in this study was the BC1 reprogrammed from peripheral blood mononuclear cells of a healthy male collected under a Johns Hopkins Institutional Review Board-approved clinical study (69).
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bioRxiv - Immunology 2020Quote: Peripheral blood samples from pediatric cancer and healthy patients were collected in EDTA tubes and peripheral blood mononuclear cells (PBMCs) were isolated from blood samples using SepMate tubes (Stemcell technology) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Freshly isolated HSPCs were resuspended in StemSpan medium (Stem Cell Technologies) supplemented with 50 ng/mL stem cell factor (SCF ...
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bioRxiv - Developmental Biology 2020Quote: ... Two conventional and established conditions were used as indicated throughout the figures and manuscript: TeSR conditions on Matrigel coated plates (Stem Cell Technologies) or FGF/KSR conditions on MEF substrate ...
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bioRxiv - Developmental Biology 2020Quote: ... hiPSCs were passaged using an enzyme-free passaging reagent (ReleSR, StemCell Technologies) and plated as small clumps.
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bioRxiv - Developmental Biology 2020Quote: hiPSCs were cultured in mTESR1 (StemCell Technologies) media or E8 medium (made in house ...
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bioRxiv - Developmental Biology 2020Quote: ... 2016) were cultivated using standard feeder-free conditions in mTeSR1 (StemCell Technologies) on Matrigel-(Corning ...