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Citations for Stemcell Technologies :
6951 - 7000 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... from healthy buffy coat donors and incubated overnight with human recombinant Interleukin 15 (STEMCELL Technologies). NK cells were incubated with immune complexes ...
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bioRxiv - Immunology 2021Quote: ... Selection kit II (STEMCELL Technologies, #17877) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Primary human CD3+ T cells were isolated from donor PBMCs via immunomagnetic negative selection (Stem Cell Tech). For thaw and culture ...
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bioRxiv - Immunology 2021Quote: ... T cells at a concentration of 2e6/ml were re-stimulated with ImmunoCult™ Human CD3/CD28/CD2 (Stemcell Technologies cat 10970) at 6.25μl/ml ...
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bioRxiv - Immunology 2021Quote: ... T cells were stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stemcell Technologies cat 10990) with 6.25μl per ml culture media at 2e6cells/ml ...
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12-Lipoxygenase Governs the Innate Immune Pathogenesis of Islet Inflammation and Autoimmune DiabetesbioRxiv - Immunology 2021Quote: ... Naïve T cells were isolated and purified from spleen and lymph nodes of C57BL/6J mice using EasySep™ Mouse CD4+ T Cell Isolation Kit (Stemcell Technologies).
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bioRxiv - Immunology 2021Quote: ... PBMCs were isolated by density centrifugation using Lymphoprep (StemCell Technologies, 07801), and magnetic beads were used for negative selection of bulk B cells (StemCell Technologies ...
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bioRxiv - Immunology 2021Quote: ... and magnetic beads were used for negative selection of bulk B cells (StemCell Technologies, 19054) or naïve B cells (17254) ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated by magnetic bead negative selection using EasySep™ Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2021Quote: ... using Gentle Cell Dissociation Reagent (GCDR, STEMCELL Technologies #07174). Each cell line was maintained below passage 45 and mycoplasma contamination was not detected ...
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bioRxiv - Bioengineering 2021Quote: ... and dissociated into single cell suspensions using Accutase (STEMCELL Technologies, #07920). One hundred and fifty thousand cells were plated onto 24-well tissue culture plates (TPP ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were activated with 1X ImmunoCult Human CD3/CD28 T Cell Activator (#10971, Stemcell Technologies) and cultured for 4 days ...
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bioRxiv - Immunology 2021Quote: Assays were performed on purified human classical monocytes isolated using immunomagnetic negative sorting (EasySep Direct Human Monocyte Isolation Kit, StemCell Technologies, Cambridge, MA). As we have previously described 88 ...
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bioRxiv - Cell Biology 2021Quote: ... tissue digests were thawed and placed in PBS containing 0.1mg/ml DNAse I solution (Stem Cell technologies, Cat#07900) to digest DNA released from dead cells ...
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bioRxiv - Cell Biology 2021Quote: ... in mTeSR1 (Stem Cell Technologies #85850) supplemented with 1% penicillin/streptomycin (P/S ...
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bioRxiv - Cell Biology 2021Quote: ... to disperse into single cells and replated in mTeSR1 supplemented with 1% P/S containing 10 µM Rock Inhibitor (Y-27632, Stem Cell Technologies #72308). A full protocol is available at the Allen Cell Explorer (www.allencell.org/methods-for-cells-in-the-lab SOP ...
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bioRxiv - Genetics 2021Quote: Cells were grown in StemSpan (StemCell Technologies) supplemented with 0.5% P/S ...
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bioRxiv - Cell Biology 2021Quote: ... Some Man13 cells were also cultured in TeSR1 medium (Stem Cell Technologies) and then transferred to E6 ...
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bioRxiv - Cell Biology 2021Quote: ... Puromycin (2.5 μg mL-1; StemCell Technologies) was added for lines stably transduced with inducible mouse MLKL constructs ...
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bioRxiv - Cell Biology 2021Quote: ... derived from different mice using a previously described method23) and selected for genomic integration using puromycin (2.5 μg mL-1; StemCell Technologies) using established procedures28 ...
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bioRxiv - Cell Biology 2021Quote: ... cells reached 40-50% confluence and were placed in E8 medium with APEL2 medium (Stem Cell Technologies, Vancouver, Canada), supplemented with 8 μM CHIR99021 ...
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bioRxiv - Immunology 2021Quote: ... Spleens of recipient mice were collected 8 days after viral infection and CD8+ T cells were purified by magnetic bead selection (EasySep, STEMCELL Technologies). Fluorescence-activated cell sorting is used for the separation of tdTomato OT-I CD8+ cells from the CD8+ T cells of recipient WT mice.
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bioRxiv - Immunology 2021Quote: ... OT-I cells were isolated with magnetic CD8+ T cell selection beads (EasySep, STEMCELL Technologies). The purity of the CD8+ T cells was assessed by flow cytometry and was >98.5% in each experiment.
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bioRxiv - Immunology 2021Quote: ... Leukocyte populations were purified from buffy coats using Lymphoprep gradient medium (Stemcell Technologies) and MACS-purification (Miltenyi CD14- ...
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bioRxiv - Immunology 2021Quote: Human peripheral blood from de-identified healthy donors was purchased from STEMCELL Technologies (Vancouver, Canada), which collects and distributes de-identified human blood products with consent forms ...
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bioRxiv - Immunology 2021Quote: ... T cells were further enriched using the EasySep Human T Cell Isolation Kit (STEMCELL Technologies), as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and 35 nM UM171 (STEMCELL technologies). Ribonucleoprotein (RNP ...
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bioRxiv - Immunology 2021Quote: ... and 1 µM SR1 (StemCell Technologies). Cells were cultured at 37°C ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s protocol (EasySepTM Human Plasmacytoid DC Enrichment kit, STEMCELL Technologies Cat#19062). Enriched HSPC-pDCs were then primed for 1-3 days in RF10 (RPMI-1640 medium (Merck ...
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bioRxiv - Immunology 2021Quote: ... CD34+ HSPCs were cultured using serum free medium SFEM II (STEMCELL Technologies) supplemented with 20 U/mL penicillin and 20 μg/mL streptomycin (Penicillin-Streptomycin ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... density-gradient centrifugation and CD34+ cells were isolated using anti-CD34 immunomagnetic beads (positive selection) following the manufacturer’s instructions (EasySepTM Human cord blood CD34+ positive selection kit II, STEMCELL Technologies Cat#17896). CD34+ HSPCs were either freshly used or cryo-preserved until future use ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s protocol (EasySepTM Human Plasmacytoid DC Enrichment kit, STEMCELL Technologies Cat#19062). Obtained cells were phenotypically validated by flow cytometry.
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bioRxiv - Immunology 2021Quote: ... 6 tissue biopsies from each endoscopic region were placed in HypoThermosol medium (Stemcell Technologies 07935) and were transported on ice to the laboratory for further processing.
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bioRxiv - Immunology 2021Quote: ... Untouched circulating leukocytes were isolated using the EasySep™ RBC Depletion Reagent (StemCell Technologies). ETA (from severe patients ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from PBMCs using EasySep™ Human NK Cell Isolation Kit (Stemcell Technologies, BC). NK cells were co-cultured with K562-NL cells at different E:T ratios and treated with 10 μM of colistin for 5 hours at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Dept of Biochemistry) and 2ng/ml TGFβ (BioTechne) on 10µg/ml of Vitronectin XF-coated plates (StemCell Technologies) at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... hiPSCs were cultured on feeder-free conditions in mTeSR Plus medium (STEMCELL Technologies, Vancouver, Canada) on Matrigel-coated culture dishes (Corning ...
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bioRxiv - Cell Biology 2021Quote: ... organoids were generated using the STEMdiff™ Cerebral Organoid Kit (STEMCELL Technologies; Cat# 08571, 08570). iPSCs were dissociated into single cells using Gentle Cell Dissociation Reagent (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... iPSCs were dissociated into single cells using Gentle Cell Dissociation Reagent (STEMCELL Technologies, cat # 07174) for 8 minutes at 37°C ...
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bioRxiv - Cell Biology 2021Quote: The STEMdiff Trilineage differentiation kit (STEMCELL Technologies, cat# 05230) was used to functionally validate the ability of newly established iPSCs to differentiate into three germ layers ...
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bioRxiv - Cell Biology 2021Quote: ... Homogeneous and reproducible EBs were generated by using a 24-well plate AggreWell™ 800 (STEMCELL Technologies, cat # 34815). On Day 7 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were dissociated into single cells using Gentle Cell Dissociation Reagent (STEMCELL Technologies, cat # 07174) for 8 minutes at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Cell Biology 2021Quote: ... hiPSCs were then incubated in imaging media of phenol red-free mTeSR1 media (Stem Cell Technologies) with 1% penicillin streptomycin (Thermo Fisher ...
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were isolated from the PBMC fraction using EasySep® naïve CD4+ T cell isolation kits and memory CD4+ T cell enrichment kits (StemCell Technologies, Meylan, France) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... ESC/iPSCs were plated in iPS-Brew with 10 μM ROCK inhibitor (Stem Cell Technologies) onto Matrigel-coated (1 mg ml-1 ...
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bioRxiv - Genomics 2021Quote: ... cells were cultured in StemMacs iPS-Brew media (Miltenyi Biotech, Auburn, CA) and routinely passaged utilizing Gentle Cell Dissociation Reagent (STEMCELL Technologies) onto Matrigel-coated (1 mg ml-1 ...
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bioRxiv - Genomics 2021Quote: ... onto Matrigel-coated (1 mg ml-1) 6-well plates 6 using ReLeSR (STEMCELL Technologies). Cells were differentiated to CD43+ hematopoietic progenitors using the StemCell Technologies Hematopoietic Kit (Cat #05310) ...
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bioRxiv - Microbiology 2021Quote: PBMCs were isolated from healthy donors using a Lymphoprep density gradient (Cat# 07861, StemCell Technologies) prior to being cultured in RPMI medium supplemented with 10% FBS and 5 U/mL penicillin/streptomycin overnight to remove monocytes via adherence ...
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bioRxiv - Microbiology 2021Quote: ... and resting CD4+ T cells (rCD4s) (EasySep™ Human Resting CD4+ T Cell Isolation Kit, Cat # 17962, StemCell Technologies) were isolated from HIV-1 aviremic donors living with HIV-1 ...