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Citations for Stemcell Technologies :
7351 - 7400 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... and for the dispersed phase the cells were used in suspension in mTeSR™l (STEMCELL Technologies) medium with Rock Inhibitor (Y-27632) ...
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bioRxiv - Bioengineering 2020Quote: ... Fluorescence microscopy (Keyence Epi-Fluorescence microscope BZ-X710, Keyence, Japan) was performed using anti-dextran FITC antibody (Stemcell Technologies, Vancouver, Canada) co-incubated with T cells for 24 h in a chamber slide for live cell imaging ...
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bioRxiv - Bioengineering 2020Quote: ... CD4pos and CD8pos T cells were isolated by negative selection using EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies), respectively ...
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bioRxiv - Bioengineering 2020Quote: ... Primary astrocyte cultures were passaged using Accutase (Stemcell Technologies).
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bioRxiv - Bioengineering 2020Quote: ... Cells were washed with PBS (-/-) and harvested with 1X Accutase (Stemcell Technologies) incubation at 37 °C for 10 min ...
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bioRxiv - Bioengineering 2020Quote: ... A hypoxic environment was created by placing the cell culture vessels in a hypoxia incubator chamber (STEMCELL Technologies, Cambridge MA), which was then filled with a gas mixture containing 2% O2 ...
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bioRxiv - Bioengineering 2020Quote: PBMCs (STEMCELL Technologies, Cambridge MA) were thawed into RPMI media (RPMI 1640 ...
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bioRxiv - Bioengineering 2020Quote: ... Media was either a 50:50 blend of conditioned media collected from perivascular hydrogel cultures and Stemspan SFEM (Stemcell Technologies) with a final concentration of 100ng mL-1 SCF (Peprotech ...
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bioRxiv - Bioengineering 2020Quote: ... Initial enrichment for HSPC was performed with the EasySep Mouse Hematopoietic Progenitor Cell Enrichment Kit and Magnet (Stemcell Technologies, CA). Further isolation was performed to obtain a population of the Lin− Sca-1+ c-kit+ (LSK ...
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bioRxiv - Bioengineering 2020Quote: T-cells were isolated with the EasySep Human T-cell isolation Kit (STEMCELL Technologies) from frozen human peripheral blood mononuclear cells obtained from healthy donors ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were thawed the day of use and kept in StemSpan SFEM II media (StemCell Technologies) with CD34 supplement (StemCell Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... supplemented with 1 μM hydrocortisone (StemCell Technologies), 2 mM L-glutamine (ThermoFisher) ...
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bioRxiv - Bioengineering 2020Quote: ... were cultured in Myelocult 5100 (StemCell Technologies) supplemented with 1 μM hydrocortisone (StemCell Technologies) ...
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bioRxiv - Bioengineering 2020Quote: HSPC (CD34+) were isolated from cord blood using the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (StemCell Technologies) according to the manufacture’s guidelines ...
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bioRxiv - Bioengineering 2020Quote: ... with CD34 supplement (StemCell Technologies) and 1 μM of StemRegenin (StemCell Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... and 1 μM of StemRegenin (StemCell Technologies) before loading into the microfluidic device ...
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bioRxiv - Biochemistry 2020Quote: CD8+ or total T cells were isolated from spleens with an EasySep(tm) T Cell Isolation Kit according to the manufacturer’s instructions (STEMCELL Technologies).
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bioRxiv - Biochemistry 2020Quote: ... In short, TJP1-GFP(ZO-1-GFP) WTCs (Allen Institute, Seattle, WA) were plated in TeSR (Stem Cell Technologies) at 200,000 cells/well of Matrigel-coated 24-well plates on day (−1) ...
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bioRxiv - Immunology 2020Quote: ... all from BD Biosciences; anti-CD38-FITC (clone AT1; 2.5 μL; cat. # 60131FI) from STEMCELL Technologies; anti-CD161-BV421 (clone HP-3G10 ...
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bioRxiv - Immunology 2020Quote: ... anti-CD38-FITC (clone AT1; 5 μL; cat. # 60131FI) from STEMCELL Technologies; and anti-CXCR5-PE (clone MU5UBEE ...
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bioRxiv - Cell Biology 2020Quote: ... PBMC were isolated from heparinized venous blood using a Lymphoprep™ medium (STEMCELL Technologies, #07851) according to standard density gradient centrifugation methods ...
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bioRxiv - Neuroscience 2020Quote: Differentiation into cerebral organoids was performed with a commercially available differentiation kit from Stemcell Technologies (Catalog number 08570-1) based on previously reported protocols [8 ...
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bioRxiv - Neuroscience 2020Quote: ... LIN28 and shRNA against p53 [28] in feeder- and serum-free conditions using TeSR-E7 medium (Stem Cell Technologies). Subsequently ...
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bioRxiv - Neuroscience 2020Quote: ... carrying genes for tdTomato and the murine cell-surface protein CD90.2/Thy1.2 driven by the endogenous POU4F2 (BRN3B) promoter were clonally propagated in mTeSR-1 media (StemCell Technologies, Cambridge, MA) on growth factor-reduced Matrigel substrate (Corning ...
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bioRxiv - Neuroscience 2020Quote: ... motor neuron progenitors (MEPs) were dissociated with Accutase (Stem Cell Technologies) and re-plated at 1:6 ratio on Matrigel-coated plate in neural medium supplemented with 3μM CHIR99021 (Tocris) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were spun down (120 × g) for 3 min and resuspended in the required amount of mTeSR (Stem Cell Technologies) + Rock Inhibitor (RI) ...
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bioRxiv - Neuroscience 2020Quote: ... the glucose concentration of LN was adjusted to 10 mM and the following supplements were added freshly before use: 20 ng/ml BDNF (Stem Cell Technologies), 20 ng/ml GDNF (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... 20 ng/ml GDNF (Stem Cell Technologies) and 1 mM db-cAMP (Santa Cruz) ...
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bioRxiv - Neuroscience 2020Quote: ... organoids were either cultured in HN+A or were transferred gradually to Low-Nutrient medium (LN) consisting of BrainPhys Neuronal Medium (Stem Cell Technologies), 2% B27+A (50X ...
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bioRxiv - Neuroscience 2020Quote: ... and the medium was replenished twice a week by replacing half of the volume with BrainPhys neuronal medium (Stemcell Technologies) supplemented with SMI and antibiotics ...
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bioRxiv - Neuroscience 2020Quote: Embryoid bodies (EB) were obtained as described [36] and using tri-lineage differentiation kit (Stem Cell Technologies). Germ layer differentiation was assessed by immunochemistry.
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bioRxiv - Biophysics 2020Quote: ... coated plates in chemically defined medium (mTeSR1™, StemCell technologies), and passaged using Dispase (StemCell technologies) ...
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bioRxiv - Biophysics 2020Quote: ... differentiation was initiated using STEMdiff™ Cardiomyocyte (CM) differentiation and Maintenance Kit (StemCell technologies). At day 15 ...
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bioRxiv - Biophysics 2020Quote: ... and passaged using Dispase (StemCell technologies). For differentiation ...
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bioRxiv - Biophysics 2020Quote: ... cell monolayers were maintained for 20-30 days in STEMDiff cardiomyocte maintenance media (STEM CELL Technologies), on EStim classic 48 well MEA plates (Axion Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... Skin was disinfected with 10% iodine in phosphate saline buffer (PBS) and incubated with 2U/ml of dispase (STEMCELL technologies) overnight at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... CD8+ T cells were isolated using RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The models were propagated in a proprietary neurosphere culture medium (NeuroCult; STEMCELL Technologies) supplemented with additional growth factors (20 ng/mL epidermal growth factor ...
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bioRxiv - Cancer Biology 2020Quote: ... and passaged by dissociating to single cells (5-minute treatment with 1X Accutase at 37 °C; STEMCELL Technologies) and resuspending at a concentration of 100k-300k cells/mL.
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bioRxiv - Cancer Biology 2020Quote: ... 25,000 cells were plated in duplicate in MethoCult GF M3434 medium (Stem Cell Technologies) and cultured at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... CellSep Human CD19+ selection kit (Stem Cell Technologies) was used to enrich for CD19+ cells ...
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bioRxiv - Cancer Biology 2020Quote: ... Viability enrichment was performed using the EasySep Dead Cell Removal (Annexin V) Kit (StemCell Technologies) as per manufacturers protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were adjusted to 1×105 cells/ml final concentration in H4230 methylcellulose medium (STEMCELL Technologies) supplemented with 10% 5637-CM as a stimulant and then plated in 35-mm petri dishes in duplicate and allowed to grow for 7 days in a humidified CO2 incubator (5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... frequency and statistics analyses were performed using L-calc software (Stemcell technologies). A p value of less than 0.05 indicates significance ...
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bioRxiv - Cancer Biology 2020Quote: ... 50U/mL DNase I (STEMCELL Technologies) at 37°C for 1h ...
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bioRxiv - Cancer Biology 2020Quote: GFP positive neural tube regions were dissected after a DispaseI-DMEM/F-12 treatment (Stem cell technologies 1U/ml #07923; 37°C, 30min). Single cell suspensions were obtained by 3 minutes incubation in Trypsin-EDTA 0.05% (Life technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... epidermal growth factor (EGF, 10 ng/ml; Stem Cell Technology) and basic fibroblast growth factor (bFGF ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 μg/ml heparin (StemCell Technologies), epidermal growth factor (EGF ...
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bioRxiv - Cancer Biology 2020Quote: Organoids were generated using the STEMdiff Cerebral Organoid Kit from Stemcell Technologies (Cat. 08570), which is based on the Lancaster et al.(Lancaster and Knoblich 2014 ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were incubated with ALDEFLUOR staining (STEMCELL TECHNOLOGIES) for 30 minutes at 37°C ...