Labshake search
Citations for Stemcell Technologies :
6901 - 6950 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Neutrophils were isolated from the leukocyte-rich plasma layer using a Easysep Neutrophil enrichment kit (STEMCELL Technologies) and placed in RPMI media ...
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bioRxiv - Immunology 2021Quote: ... negative selection was performed using EasySep Human T cell isolation kit (#17951, Stemcell technologies, Vancouver, Canada). The CD3+ T cells were labelled with 1µM Cell Trace Violet dye (#C34557 ...
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bioRxiv - Immunology 2021Quote: PBMCs were isolated from peripheral blood using Lymphoprep (Stemcell technologies, Vancouver, Canada) density gradient medium ...
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bioRxiv - Immunology 2021Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×10^6 T cells/ml in serum-free StemCell Immunocult™-XF media (StemCell Technologies, Vancouver, Canada) with clinical grade 20U/ml human IL-2 (Novartis)
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bioRxiv - Immunology 2021Quote: ... Enriched cells were stained 30 min on ice in a RoboSep buffer (StemCell Technologies) containing following phenotyping antibodies ...
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bioRxiv - Immunology 2021Quote: ... plasmablasts were enriched from the whole blood (day 7 after second vaccination) by negative selection using custom direct human plasmablasts isolation kit containing paramagnetic beads and antibodies for negative selection (StemCell Technologies). Enriched cells were stained 30 min on ice in a RoboSep buffer (StemCell Technologies ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were enriched from whole blood (day 10 after first, and day 7 after second vaccination) using direct PBMCs isolation kit (StemCell Technologies). For singe-cell antibody secretion and paired antibody sequencing studies ...
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bioRxiv - Immunology 2021Quote: ... and differentiated in ALI culture conditions with PneumaCultTM-ALI Medium (STEMCELL Technologies). All the human tissue-related work was approved by the ethics committee of the Seventh Affiliated Hospital of Sun Yat-sen University ...
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bioRxiv - Immunology 2021Quote: ... with 500 μl of fresh serum-free growth medium (05001, STEMCELL Technologies) or CIPS-containing medium ...
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bioRxiv - Immunology 2021Quote: ... and expanded using PneumaCultTM-Ex Plus Medium (STEMCELL Technologies). After progenitor cell expansion ...
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bioRxiv - Immunology 2021Quote: ... The tissue pieces were dissociated with Dispase I (STEMCELL Technologies, Vancouver, Canada) and centrifuged to collect dissociated cells ...
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bioRxiv - Immunology 2021Quote: Memory and effector CD4 T cells were purified from total splenocytes isolated from immunized mice using EasySepTM Mouse Memory CD4+ T Cell Isolation Kit (StemCell Technologies) as per manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... Samples were minced and incubated with Stem Cell Spleen dissociation media (Stem Cell Technologies, Vancouver, British Columbia, Canada) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated using ImmunoCult™ Human CD3/CD28 T Cell Activator antibodies (StemCell Technologies).
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bioRxiv - Immunology 2021Quote: ... Cells were lifted from the plate by 15 minute incubation at 37°C with Accutase™ (StemCell Technologies), then washed with PBS + 2% FCS ...
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bioRxiv - Neuroscience 2021Quote: ... Isotype control was stained using control antibody (STEMCELL Technologies, 60070AD.1, 0.2 ug/ul) at 1:378 dilution for 10 min at 4 °C protected from light ...
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bioRxiv - Immunology 2021Quote: PBMCs were isolated from whole-blood specimens using Lymphoprep density gradient medium according to the manufacturer’s protocol (StemCell Technologies, Vancouver, BC, Canada). LN specimens were obtained by FNA biopsies of 1–2 lymph nodes in the inguinal chain area ...
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bioRxiv - Immunology 2021Quote: ... In vitro experiments were performed using human mononuclear cells from peripheral blood or synovial fluid (SF) isolated by centrifugation in density gradient medium (STEMCELL Technologies). Human monocytes were purified by positive selection with Human CD14 microbeads (Miltenyi) ...
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bioRxiv - Immunology 2021Quote: ... following informed written consent (Stemcell Technologies). Bulk T cells were isolated from Leukopaks using EasySep magnetic selection following manufacturers’ recommended protocol (Stemcell Technologies cat 17951) ...
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bioRxiv - Immunology 2021Quote: ... Bulk T cells were isolated from Leukopaks using EasySep magnetic selection following manufacturers’ recommended protocol (Stemcell Technologies cat 17951). Immediately following isolation ...
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bioRxiv - Immunology 2021Quote: ... samples were subjected to EasySep™ dead cell removal per manufacturer’s protocol (Stemcell Technologies). If the suspension had considerable debris ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were isolated from blood using LymphoprepTM (StemCell Technologies), and then cryopreserved in 90% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: PBMCs from three independent donors were isolated from leukapheresis products using Lymphoprep density gradient centrifugation and SepMate PBMC isolation tubes (StemCell Technologies), under the Weatherall Institute of Molecular Medicine ...
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bioRxiv - Immunology 2021Quote: ... CD3+ T cells or CD4+ T cells were isolated via Lymphoprep and RosetteSep (both STEMCELL Technologies). For Treg isolation ...
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bioRxiv - Immunology 2021Quote: ... 5mL of whole blood was enriched for CD4+ cells using RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technology). CD4+ cells were frozen in 10% DMSO/FBS and stored in liquid nitrogen ...
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bioRxiv - Microbiology 2021Quote: ... and CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads per the manufacturer’s instructions (StemCell Technologies) and were activated with phytohemagglutinin-L (10 μg/mL ...
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bioRxiv - Microbiology 2021Quote: Both primary and BCi-NS1.1 airway epithelial cells were first expanded in Pneumacult-Ex or Pneumacult-Ex Plus medium (#05008, #05040; StemCell Technologies), and differentiated in Pneumacult-ALI medium (#05001 ...
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bioRxiv - Immunology 2021Quote: ... Monocytes were isolated by negative selection using the monocyte enrichment kit from STEMCELL Technologies (Cambridge, MA). Cell purity was confirmed by flow cytometry using CD14+ staining and was found to be >90% ...
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bioRxiv - Immunology 2021Quote: ... followed by enrichment of either effector/memory T cells (Human Memory CD4 T cell Enrichment kit; EasySep) or Treg cells (Human CD4+CD127loCD49d− Treg Enrichment Kit; EasySep) (all from StemCell Technologies).
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bioRxiv - Immunology 2021Quote: ... The selected cloned cell lines secreting mAb H5.28 or H5.31 were grown initially in hybridoma growth medium (ClonaCell-HY medium E from STEMCELL Technologies). Prior to antibody expression and purification ...
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bioRxiv - Immunology 2021Quote: Purified CD4+CD25− T cells were magnetically isolated from spleen and peripheral lymph node mononuclear cells using an EasySep magnetic T cell negative isolation kit (Stem Cell Technologies, Inc.) with addition of a biotin anti-mouse CD25 antibody (0.5 μg/mL ...
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bioRxiv - Immunology 2021Quote: ... Resting B cells were isolated using the EasySep Negative Selection- Mouse B Cell Isolation Kit (STEMCELL Technologies), which depletes for non-B cell markers and activated CD43+ B cells ...
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bioRxiv - Immunology 2021Quote: ... Naive CD4+ T cells were isolated by negative selection with the EasySep Human Naïve CD4+ T Cell Isolation Kit (STEMCELL Technologies) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and HetaSep (STEMCELL Technologies) to remove red blood cells ...
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bioRxiv - Microbiology 2021Quote: ... were detached using dispase (STEMCELL Technologies) and maintained in human embryonic stem cell (hESC ...
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bioRxiv - Microbiology 2021Quote: ... Cells were maintained in feeder-free conditions in mTeSR1 culture medium (Stem Cell Technologies) on matrigel ES (Corning ...
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bioRxiv - Microbiology 2021Quote: Nasal brushings were placed in PneumaCult-Ex Plus medium (STEMCELL Technologies, Cambridge, UK) and cells dissociated from the brush by gentle agitation ...
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bioRxiv - Immunology 2021Quote: ... and Treg isolation kits were purchased from Stem Cell Technologies (Vancouver, Canada). Recombinant IL-2 ...
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells (PBMCs) were isolated at RT using SepMate PBMC Isolation Tubes (STEMCELL Technologies) by the UCSF Biospecimen Resource Program ...
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bioRxiv - Immunology 2021Quote: ... The pellet was washed twice each time with 20 mL of EasySep buffer (STEMCELL Technologies) followed by centrifugation at 400 rcf for 10 min with brakes on ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in PBS and incubated in PBS + 50 nM dasatinib (Stem Cell Technologies, Canada) + DNAse at 37°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... Remaining blood was diluted with 8mL of DPBS and slowly added to a SepMate-50 tube pre-filled with 15mL of Lymphoprep (STEMCELL Technologies). The tube was then centrifuged at 800 rcf for 20 min with brakes off ...
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bioRxiv - Immunology 2021Quote: ... In vitro experiments were performed using human plasmacytoid dendritic cells (pDCs) purified from PBMCs by negative selection with the EasySep Human Plasmacytoid DC enrichment kit (STEMCELL Technologies). pDCs were cultured in RPMI 1640 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... CD4+CD45RA+ naïve T cells were isolated using negative enrichment kits (Stemcell Technologies, Vancouver, Canada) per manufacturer’s instructions and confirmed >90% pure by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... and treated with Gentle Cell Dissociation Reagent (STEMCELL Tech) for 15 min at room temperature (RT ...
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bioRxiv - Immunology 2021Quote: ... and 0.1 U/ml dispase (STEMCELL Technologies, #07913) in DMEM supplemented with 10% FCS ...
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bioRxiv - Immunology 2021Quote: ... Th17 cells were enriched (StemCell Technologies; 17862) from healthy donor human PBMCs and cultured in 96 well plates in IMDM supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... naïve CD4+ T cells were enriched (StemCell Technologies; 19555) from healthy donor human PBMCs and cultured in 96-well plates ...
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bioRxiv - Immunology 2021Quote: ... Human NK cells were isolated the evening before using RosetteSep Human NK cell Enrichment cocktail (STEMCELL Technologies) from healthy buffy coat donors and incubated overnight with human recombinant Interleukin 15 (STEMCELL Technologies) ...
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bioRxiv - Immunology 2021Quote: ... counted and treated with 10 U mL−1 DNase I (STEMCELL Technologies, #07900) for 15 min at RT to prevent cell clumping ...