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Citations for Stemcell Technologies :
7201 - 7250 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... hESCs were cultured in mTeSR1 media (Stem Cell Technologies) and propagated by passaging cells every 3 to 4 days using Accutase (eBioscience ...
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bioRxiv - Genomics 2020Quote: ... detached using Accutase (Stem Cell Technologies, Vancouver, Canada), quenched with full cold media ...
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bioRxiv - Genomics 2020Quote: KO cell lines generated using nucleofection of Cas9 RNP were detached with Accutase (Stem Cell Technologies, Vancouver, Canada) and stained with APC conjugated antibodies targeting B2M (2M2) ...
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bioRxiv - Genomics 2020Quote: ... 6% v/v lymphoprep (STEMCELL Technologies, Canada), 1M betaine (#B0300-1VL ...
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bioRxiv - Genomics 2020Quote: ... Cells were first differentiated into definitive endoderm using the STEMdiff Definitive Endoderm Kit (Stem Cell Technologies) for 72 hours and subsequently dissociated with GCDR and passaged as small clumps into growth factor reduced Matrigel-coated (Corning ...
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bioRxiv - Genomics 2020Quote: ... in mTeSR1 medium (Stem Cell Technologies) and passaged using gentle cell dissociation reagent (GCDR) ...
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bioRxiv - Developmental Biology 2020Quote: ... and maintained in mTESR1 media (Stem Cell Technologies) at 37°C with 5% CO2 ...
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bioRxiv - Developmental Biology 2020Quote: ... hPSC lines were maintained on feeder-free conditions in mTeSR1 medium (StemCell technologies, Vancouver, Canada) on six-well Nunclon surface plates (Nunc ...
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bioRxiv - Genomics 2020Quote: ... pre-coated culture dishes and fed with mTeSR (Stem cell Technologies) supplemented with 100 I.U./ml penicillin and 100 μg/ml streptomycin ...
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bioRxiv - Genomics 2020Quote: ... CD34+ human cord blood cells were obtained from StemCell Technologies, and long-term CD34+ HSPC cultures were established by overexpression of AML1-ETO or AML1-ETO-FKBP12F36V as previously described23 ...
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bioRxiv - Genomics 2020Quote: ... Aspirated off the supernatant and resuspended pellet in 1 ml Accumax (Stemcell Technologies, 07921) for 3 min at 37°C ...
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bioRxiv - Genomics 2020Quote: ... Tissues were minced into ~1-2 mm pieces and digested with 1× Collagenase/Hyaluronidase (STEMCELL Technologies) and 100 μg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: The Aldefluor kit (Stem Cell Technologies) was used to characterize the ALDH activity of 4T1 cells as described in (Pearce et al ...
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bioRxiv - Microbiology 2020Quote: ... containing MyeloCult H5100 (Stem Cell Technologies, cat#5150) supplemented with 1 μM hydrocortisone ...
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bioRxiv - Cancer Biology 2020Quote: ... Organoids were cultured in IntesticultTM Organoid Growth media (06005, Stem Cell Technologies). The Rho kinase inhibitor Y-27632 (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... 250 µl of IntesticultTM Organoid Growth media (06005, Stem Cell Technologies) (for APCmin organoids ...
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bioRxiv - Cancer Biology 2020Quote: ... 250 µl of IntesticultTM Organoid Growth media (06005, Stem Cell Technologies) (for APCmin organoids ...
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bioRxiv - Cell Biology 2020Quote: ... 1,000-1,200 sort-purified CD34-subpopulations were seeded into MethoCult H4435 (Stemcell Technologies) or H4230 supplemented with hIL-3 ...
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bioRxiv - Immunology 2020Quote: ... 8 weeks old males (Stem Cell Technologies #19765), according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... Semisolid culture medium was prepared by mixing methylcellulose (MethoCult™ SF M3236; Stem Cell Technologies), 15% fetal bovine serum ...
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bioRxiv - Genetics 2020Quote: ... Cryopreserved CD34+ cells were thawed and pre-stimulated for 48 h in StemSpan SFEM (StemCell Technologies) supplemented with 100 ng/mL SCF ...
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bioRxiv - Genomics 2020Quote: ... they were passaged using ReLeSR (STEMCELL Technologies, 05873) according to manufacturer’s guidelines.
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bioRxiv - Genomics 2020Quote: ... and batch fed daily with mTeSR (STEMCELL Technologies, 85850). Once the cells had reached confluency ...
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bioRxiv - Physiology 2020Quote: ... and 5 μM Xav-939 (Stem Cell Technologies). On day 5 ...
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bioRxiv - Physiology 2020Quote: ... containing 3 μM CHIR99021 (Stem Cell Technologies), 500 μg/mL BSA (Sigma Aldrich) ...
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bioRxiv - Physiology 2020Quote: ... Single-cell suspensions were plated at a density of 1.2 × 105 cells/cm2 and cultured overnight in mTeSR medium supplemented with 10 μM Y-27632 dihydrochloride (Stem Cell Technologies). Once the monolayer reached approximately 80% confluence (usually the following day) ...
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bioRxiv - Physiology 2020Quote: ... coated tissue culture dishes as per manufacturer recommendation with either mTeSR medium with supplementation or mTeSR PLUS medium with supplementation (Stem Cell Technologies). Contractile cardiomyocytes were differentiated using a high-density monolayer format as previously described (43) ...
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bioRxiv - Physiology 2020Quote: ... Undifferentiated hiPSCs were maintained on Vitronectin XF (5 μg/mL, Stem Cell Technologies) coated tissue culture dishes as per manufacturer recommendation with either mTeSR medium with supplementation or mTeSR PLUS medium with supplementation (Stem Cell Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: Flow sorted cells were seeded in serum-free methylcellulose-based medium with cytokines (MethoCult SFH4436, Stemcell technologies). 5 μM imiquimod or DMSO were added to the methocult at the time of seeding in a separate location within the media prior to vortexing ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 μg of DNase I (07900; StemCell Technologies) per mL of cell suspension was added to prevent clumping.
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bioRxiv - Cancer Biology 2020Quote: Frozen human bone marrow mononuclear cells were thawed and washed twice with Iscove’s Modified Dulbecco’s Medium (StemCell Technologies) containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2020Quote: ... and SepMate(tm) tubes (StemCell Technologies).
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bioRxiv - Cancer Biology 2020Quote: Fresh bone marrow was collected into EDTA and purified within 12 hours of collection by negative selection using the RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail according to the manufacturer’s protocol (15026, StemCell Technologies, Cambridge, UK), followed by density gradient centrifugations with Ficoll-Paque Premium (GE Healthcare Life Sciences ...
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bioRxiv - Developmental Biology 2020Quote: ... Colonies were passaged using the gentle cell dissociation reagent (07174, StemCell Technologies). All cell lines were regularly tested for Mycoplasma contamination by PCR.
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bioRxiv - Developmental Biology 2020Quote: HESCs (line H9) were maintained in mTeSR1 medium (05850, STEMCELL Technologies) on Matrigel-coated plates (356234 ...
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bioRxiv - Bioengineering 2020Quote: ... Blood was then washed with RBC Lysis Buffer (Stem Cell Technologies), then centrifuged at 16 000 g for 10 min ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... setting the instrument to deposit one cell into each well in a 96-well plate containing working medium with 10% CloneR cloning supplement (Stemcell Technologies). Filled plates were transferred to the 37°C incubator and left undisturbed for 48 hours after sorting ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... cells were maintained on 6-well plates in a humidified incubator at 37°C with 5% CO2 and passaged every 3-4 days (at ∼80% confluence) using ReLeSR (Stemcell Technologies), following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: Cells were dissociated using the CM dissociation kit (Stem Cell Technologies, #05025). After centrifugation for 3 min at 130g ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were grown on either Corning or Eppendorf tissue culture-treated plates coated with Vitronectin XF (Stem Cell Technologies #7180) and passaged using either TrypLE Express Enzyme (Gibco ...
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bioRxiv - Immunology 2020Quote: Isolated IEL were further enriched using an EasySep™ Mouse CD8α positive selection kit (STEMCELL technologies) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... an EasySep™ Release PE positive selection kit (STEMCELL Technologies) was used with a PE-conjugated anti-mouse CD8α antibody (BioLegend ...
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bioRxiv - Cancer Biology 2020Quote: ... EGF ligand was purchased from Stem cell technology.
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bioRxiv - Immunology 2020Quote: ... Monocytes were isolated from PBMC by negative selection using the EasySep™ Human Monocyte Isolation Kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... the EasySep Kit (StemCell Technologies, cat. 18000) was used according to manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... and Monocyte negative selection kit (STEMCELL Technologies, https://www.stemcell.com), respectively ...
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bioRxiv - Microbiology 2020Quote: ... PE positive selection kit (STEMCELL Technologies, https://www.stemcell.com) and Monocyte negative selection kit (STEMCELL Technologies ...
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bioRxiv - Microbiology 2020Quote: ... CD8 positive selection kit (STEMCELL Technologies, https://www.stemcell.com), PE positive selection kit (STEMCELL Technologies ...
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bioRxiv - Microbiology 2020Quote: ... monocytes and others (CD4− CD8− CD19− cells) using CD4 negative selection kit (STEMCELL Technologies, https://www.stemcell.com), CD8 positive selection kit (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... Dispase II (2.5 U/mL; STEMCELL Technologies), fatty acid free bovine serum albumin (2% ...