Labshake search
Citations for Stemcell Technologies :
6851 - 6900 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... and maintained in mTesr1 medium (STEMCELL Technologies) every day ...
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bioRxiv - Biophysics 2021Quote: ... cells were maintained in mTesr media (Stem Cell Technologies) and three days before differentiation ...
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bioRxiv - Cancer Biology 2021Quote: ... as previously described by Sato et al.79 Organoid culture media (OCM) Human Intesticult™ (Stemcell Technologies Inc, Canada) containing 0.2% Primocin™ (Invivogen ...
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bioRxiv - Cancer Biology 2021Quote: ... recombinant mouse Interleukin-3 (6 ng/ml) and recombinant human Interleukin-6 (10 ng/ml) (all from Stem Cell Technologies). 200ul fresh SFEM medium with cytokines was added on Day 5 and 8 and cells were counted on Day 5 and 10.
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bioRxiv - Cancer Biology 2021Quote: ... Total CD8+ T cells were isolated from unfractionated PBMCs using the EasySep Human CD8+ T Cell Isolation Kit (Stem Cell Technologies) with a DynaMag-2 magnet (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... SCLC aggregates were generated using Aggrewell 800 (Stem Cell Technologies). 3×106 N2N1G cells were added to each well and centrifuged for 5 min at 100xg ...
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bioRxiv - Cancer Biology 2021Quote: ... and immersed in EpiCult-B Medium (STEMCELL Technologies, 05610) supplemented with 10 ng/mL human recombinant EGF (PeproTech ...
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bioRxiv - Cancer Biology 2021Quote: ... before undergoing tissue dissociation with a GentleMACS™ dissociator (Miltenyi Biotech) using the manufacturer’s recommended protocol with media including Collagenase Type IV (STEMCELL Technologies, Cambridge MA) and DNAse I (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... Blood-soaked tissue samples were washed with an ammonium chloride solution (Stemcell Technologies, Canada) to lyse present erythrocytes ...
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bioRxiv - Microbiology 2021Quote: ... dead cells were removed by Dead Removal cocktail (Annexin V, STEMCELL Technology). Live cells were then used 10X GEM generation ...
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bioRxiv - Molecular Biology 2021Quote: ... neurospheres were dissociated to single cell suspension (Stemcell Technologies) and transfected with the oligonucleotides using Neuromag 500 (OZ Biosciences).
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bioRxiv - Molecular Biology 2021Quote: H9 hESCs were purchased from the National Collection of Authenticated Cell Cultures (Shanghai, Catalog SCSP-302) and were maintained in mTeSR (Stem Cell Technology) on Matrigel-coated plates at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... differentiation of hESCs cardiomyocytes was performed using the STEMdiffTM Cardiomyocyte Differentiation Kit (Stem Cell Technology), whereas analysis of cardiomyocyte induction efficiency was conducted via FACS using the cTnT+ primary antibody (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: Single cell suspensions were prepared from multiple OT-I and OT-I;IL-7Rα449F mice spleens and CD8 T cells were purified using the CD8 T cell negative selection kit (EasySep™ Mouse CD8+ T Cell Isolation Kit) from Stem Cell Technologies. 1×106 cells were transferred I.V ...
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bioRxiv - Immunology 2021Quote: ... CD8+ and CD4+ cells were enriched from the non-adherent fraction by untouched negative selection (Stemcell Technologies, Cat 17952 and Cat 17953). To generate moDC ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from whole blood from healthy donors (as mentioned above) by negative selection using RosetteSep (STEMCELL Technologies) then separated using a ficoll gradient ...
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bioRxiv - Immunology 2021Quote: The 7D9 hybridoma line was cultured in ClonaCell™-HY Medium E (STEMCELL Technologies) prior to antibody production ...
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bioRxiv - Immunology 2021Quote: ... Untouched CD8+ T cells were magnetically isolated using EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, Grenoble, France) or MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend ...
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bioRxiv - Immunology 2021Quote: Naïve CD8 T cells were isolated from young female (6w-8w) B6 mouse lymph nodes and spleens with the EasySep Mouse Naïve CD8 T Cell Isolation Kit (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Cells from all mice were pooled by tissue type and B cells were selected from lymph node and spleen using a mouse Pan-B negative selection kit (Stemcell Technologies, Vancouver, BC, Canada). Generation of natively paired heavy and light chain libraries ...
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bioRxiv - Immunology 2021Quote: ... or by using the EasySep™ mouse CD19 positive selection kit (STEMCELL Technologies) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and human BAFF(71) in 80 mL StemCell medium A (StemCell Technologies) containing 6.3 µg/mL of CpG (phosphorothioate-modified oligodeoxynucleotide ZOEZOEZZZZZOEEZOEZZZT ...
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bioRxiv - Immunology 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by density centrifugation from peripheral blood leukocyte separation medium (STEMCELL Technologies). In vitro experiments were performed using human plasmacytoid dendritic cells (pDCs ...
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bioRxiv - Immunology 2021Quote: ... CD4+ CD62L+ naïve T cells were isolated from 8-12 week-old mice using CD4 negative enrichment kits (Stemcell Technologies, Vancouver, Canada) and CD62L microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... PBMC were cryopreserved in Cryostor10 (StemCell Technologies #07930) or 90% FBS (ThermoFisher #10438026 ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: Total CD4+ T cells were purified with EasySep Mouse T Cell Isolation Kit (Stemcell Technology). Total splenocytes suspended in MACS buffer (2% FBS in DPBS ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were purified from whole blood of anemic and non-anemic patients by density separation using Lymphoprep (STEMCELL Technologies).
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bioRxiv - Immunology 2021Quote: ... Collected PBSCs were subjected to density gradient centrifugation using Lymphoprep (STEMCELL Technologies) to remove dead cells and debris ...
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bioRxiv - Immunology 2021Quote: Human B cells were enriched from cryopreserved PBMCs using the EasySep™ Human B Cell Enrichment Kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from EDTA-treated blood by density centrifugation (Lymphoprep, STEMCELL Technologies Inc). After washing ...
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bioRxiv - Immunology 2021Quote: Naive P14 CD8+ T cells from wildtype mice were isolated by negative selection (EasySep™, Stemcell Technologies). Naive P14 Thy1.1+ Il2ra−/− cells were isolated from 4-5 week old mice by depleting CD44hi cells (Biolegend biotin anti-mouse/human CD44 ...
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bioRxiv - Immunology 2021Quote: ... and PBMC’s were isolated by density gradient centrifugation (Lymphoprep, StemCell Technologies). Residual palatine tonsil samples were acquired from pediatric patients ...
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bioRxiv - Immunology 2021Quote: ... Tonsil mononuclear cells were isolated by density gradient centrifugation (Lymphoprep, StemCell Technologies). PBMC’s from pediatric donors were obtained during surgery with informed consent granted by a parent/guardian ...
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bioRxiv - Immunology 2021Quote: De-identified peripheral blood units were purchased from StemCell Technologies (Vancouver, Canada). Peripheral blood samples from 25 healthy donors (11 male and 14 female ...
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bioRxiv - Immunology 2021Quote: ... The preparation was centrifuged (200 g; 7 min) and the pellet resuspended in PneumaCult-Ex Plus expansion medium (Stemcell Technologies), then seeded onto 25 cm2 tissue culture flasks pre-coated with 30 µg/mL Type I collagen (PureCol ...
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bioRxiv - Immunology 2021Quote: ... apical medium was removed and basolateral medium was switched to PneumaCult-ALI-S (Stemcell Technologies). Cells were maintained at air-liquid interface until fully differentiated ...
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bioRxiv - Immunology 2021Quote: ... CD4 T cells (non-irradiated) were isolated by magnetic bead selection (Cell Stemcell Technologies). Cells were cultured for 72 hours and flow cytometric analysis used to assess proliferation as measured by CFSE dilution.
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bioRxiv - Immunology 2021Quote: ... 100μL of Isolation Cocktail and 100μL of Rapid Spheres (Easy Sep™ Direct Human Total Lymphocytes Isolation Kit: 19655, StemCell Technologies) were added to the remaining whole blood ...
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bioRxiv - Immunology 2021Quote: Whole blood was collected in heparin containing vacuutubes and 12μL of 0.5M EDTA with 1mL of PBS+2% FBS and 50μL of RBC of EasySep RBC Depletion spheres (EasySep ™ RBC Depletion Reagent: 18170, Stem Cell Technologies) were added ...
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bioRxiv - Immunology 2021Quote: ... CD34+ cells were isolated using the EasySep Human CD34+ Positive Selection Kit (Stemcell Technologies) to > 90% purity as previously described (Supplemental Figure 1)20 ...
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bioRxiv - Immunology 2021Quote: ... 1 μg/ml low-density lipoprotein (LDL; Stemcell Technologies), 60 μM ascorbic acid (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... then processed with EasySep Mouse Neutrophil Enrichment Kit (StemCell Technologies) to isolate neutrophils by negative selection ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in 100-200 μl of Iscove’s Modified Eagle Medium (IMDM) supplemented with 20% serum substitute (BIT 9500; Stemcell Technologies), 1 μg/ml low-density lipoprotein (LDL ...
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bioRxiv - Immunology 2021Quote: ... and CD4+ T cells were pre-activated with either CD3/CD28/CD2 tetrameric antibody complexes (ImmunoCult by STEMCELL Technologies) or 1:1 aAPCs for 5 d ...
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bioRxiv - Immunology 2021Quote: ... CD3/CD28 tetrameric antibody complexes (ImmunoCult by STEMCELL Technologies), or a combination of plate-bound anti-CD3 (OKT3 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were cultivated in StemFlex™ cell culture medium supplemented with ROCK inhibitor (STEMCELL Technologies) and HDR enhancer (Integrated DNA Technologies).
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bioRxiv - Molecular Biology 2021Quote: ... into the STEMdiff SMADi neural induction medium (StemCell Technologies). Cells were passaged this way once a week ...
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bioRxiv - Molecular Biology 2021Quote: ... by density gradient centrifugation (Stemcell Technologies, Vancouver, Canada). For T-cell experiments ...
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bioRxiv - Immunology 2021Quote: ... Blood was then incubated with Hetasep (STEMCELL Technologies) according to manufacturer protocols to deplete red blood cells and enrich leukocytes ...
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells were isolated using Lymphoprep (Stemcell technologies, Canada). Monocytes were positively selected by anti-CD14-coated magnetic beads (Miltenyi biotec ...