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Citations for Stemcell Technologies :
501 - 550 of 1526 citations for T complex protein 1 subunit epsilon CCT5 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... CD4+ T lymphocytes were purified using immunomagnetic beads as per the manufacturer’s instructions (StemCell Technologies). CD4+ T lymphocytes were activated with phytohemagglutinin-L (PHA-L ...
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bioRxiv - Neuroscience 2021Quote: ... Isotype control was stained using control antibody (STEMCELL Technologies, 60070AD.1, 0.2 ug/ul) at 1:378 dilution for 10 min at 4 °C protected from light ...
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bioRxiv - Bioengineering 2023Quote: ... The following primary antibodies were used: beta-tubulin III (STEMCELL Technologies 60052; 1:1000), GFAP (Dako GA524 ...
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bioRxiv - Immunology 2021Quote: ... T-cells were isolated with an EasySep CD3+ enrichment kit from StemCell Technologies (Vancouver, BC Canada), per the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: Primary human T cells were isolated by negative selection for CD3+ populations (STEMCELL technologies, Easy Sep) from anonymous healthy human donor blood collected by leukopheresis and purchased from STEMCELL technologies ...
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bioRxiv - Immunology 2021Quote: ... Human CD8+ T cells were cultured in ImmunoCult cell culture media (Stemcell Technologies, Cat no. 10981) supplemented with 35 ng/ml human IL-2 (Peprotech ...
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bioRxiv - Bioengineering 2021Quote: ... 25 µL/mL of ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stemcell Technologies - #10970) were used ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were enriched using an EasySep magnetic negative selection kit (Stem Cell Technologies #19853) as per the manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: from CD4-T cells following the protocol described in the EasySep PE-selection kit (STEMCELL technologies). Cells from the negative fraction (CD4+CD25- ...
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bioRxiv - Cancer Biology 2020Quote: ... Immuno-magnetic T and B cell depletion was performed using the EasySep magnetic system (StemCell Technologies), prior to setting up in vitro cultures ...
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bioRxiv - Immunology 2021Quote: ... negative selection was performed using EasySep Human T cell isolation kit (#17951, Stemcell technologies, Vancouver, Canada). The CD3+ T cells were labelled with 1µM Cell Trace Violet dye (#C34557 ...
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bioRxiv - Immunology 2023Quote: ... human CD4+ T cells were purified from frozen peripheral blood mono-nuclear cells (PBMCs) (STEMCELL Technologies) with EasySep Human CD4+ T Cell Isolation Kit (STEMCELL Technologies) ...
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bioRxiv - Bioengineering 2024Quote: ... Tregs were isolated using the EasySep human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (StemCell Tech.) and cultured in X-Vivo15 ...
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bioRxiv - Immunology 2022Quote: ... and CD8+ T cells were subsequently isolated using the Stemcell human CD8+ isolation kit (Stemcell Technology) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... responder CD4+ T cells were isolated from OTII BL/6 mice by negative selection (STEMCELL Technologies). Splenocytes from wild-type or HLA-A2+ BL/6 mice were depleted of Thy1.2+ cells (STEMCELL Technologies) ...
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bioRxiv - Bioengineering 2023Quote: ... ImmunoCult-XF T Cell Expansion Medium and immunomagnetic negative selection kits were purchased from STEMCELL Technologies. FITC-dextran molecules (500 kDa ...
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bioRxiv - Genomics 2024Quote: ... as described above for Jurkat T cells) supplemented with 50 U/ml of IL2 (StemCell Technologies), or cryopreserved in BamBanker Freezing Media (Bulldog Bio) ...
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bioRxiv - Immunology 2024Quote: ... splenocytes were isolated were magnetically enriched using the CD4+ T cell negative selection kit (STEMCELL Technologies). For analysis at 7 ...
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bioRxiv - Bioengineering 2024Quote: ... then dissociated and Cd4+/CD8+ T cells were isolated using EasySepTM Mouse isolation kit (Stemcell Technologies) according to manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: ... voluntary healthy blood donors utilizing the RosetteSep Human CD8+ T cell Enrichment Cocktail (STEMCELL technologies, Germany) negative selection kit following the manufacturers instruction ...
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bioRxiv - Cell Biology 2024Quote: ... suspension cultures of 1.0×105 primary human peripheral blood-derived CD4+ T cells (Stem Cell Technologies) were grown in RPMI-1640 with 10% FBS 1% P/S (Gibco ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells were purified from CD45.1+ CD45.2+ or CD45.2+ SMARTA splenocytes by negative selection (STEMCELL Technologies) and stimulated in 12-well plates pre-coated with 8 μg/mL anti-CD3 (145-2C11 ...
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bioRxiv - Immunology 2021Quote: ... isolated T-IEL were further enriched using an EasySep™ Mouse CD8α positive selection kit (STEMCELL technologies) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cells were left alone or activated using Immunocult human CD3/CD28 T Cell Activator (Stem Cell Technologies) for 15 min ...
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bioRxiv - Immunology 2020Quote: ... CAR-transduced T cells were enriched by positive selections using anti-CD19 magnetic beads (Stem Cell Technologies). Cultures were propagated for 14-16 days before applying to assays or get cryo-preserved ...
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bioRxiv - Immunology 2020Quote: Naïve CD4+ T cells were negatively isolated from fresh PBMCs by EasySep (Stem Cell Technologies; >95% purity). Cells were stimulated (Ellis et al. ...
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bioRxiv - Immunology 2020Quote: ... or αCD3/CD28 per manufacturer’s instructions (ImmuCult human CD3/CD28 T cell activator; StemCell Technologies, Cambridge, MA). After stimulation ...
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bioRxiv - Immunology 2020Quote: ... were purified via negative selection (EasySep™ Mouse CD4+ T cell Isolation Kit, STEMCELL Technologies, Lot: 18H94653). Purified CD4+ T cells were cultured for 72 hours with interleukin-12 (IL-12 ...
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bioRxiv - Genomics 2021Quote: ... Frozen vials of primary human CD4+CD45RO+ memory T cells were purchased from STEMCELL Technologies (Cat #70031).
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bioRxiv - Immunology 2020Quote: ... Control T cells were isolated from from leukapheresis products from healthy donors (STEMCELL Technologies, Vancouver, BC, Canada). This study was approved by the Research Ethics Board of the BC Cancer Agency/University of British Columbia (certificates H16-00291-A021 ...
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bioRxiv - Immunology 2020Quote: ... For Il2ra time course naïve CD4+ T cells were enriched from splenocytes using EasySep kit (StemCell Technologies). Approximately 100,000 cells were activated using 2ug/ml plate bound anti-CD3/CD28 ...
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bioRxiv - Immunology 2021Quote: Naive P14 CD8+ T cells from wildtype mice were isolated by negative selection (EasySep™, Stemcell Technologies). Naive P14 Thy1.1+ Il2ra−/− cells were isolated from 4-5 week old mice by depleting CD44hi cells (Biolegend biotin anti-mouse/human CD44 ...
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bioRxiv - Immunology 2021Quote: Primary human CD3+ T cells were isolated from donor PBMCs via immunomagnetic negative selection (Stem Cell Tech). For thaw and culture ...
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bioRxiv - Immunology 2023Quote: ... Lymph node CD8α+ T cells were isolated and sorted using the CD8α Positive Selection Kit (Stemcell Technologies). Isolated cells were washed and resuspended at 1 x 106 cells/ml in stimulation media ...
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bioRxiv - Genomics 2023Quote: Primary CD25-CD4+ effector T cells were isolated from fresh Human Peripheral Blood Leukopaks (STEMCELL Technologies, #70500) from healthy donors ...
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bioRxiv - Immunology 2023Quote: ... and monocytes were isolated from PBMCs by EasySep Human T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada), MACSxpress Whole Blood NK Cell Isolation Kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Cells were enriched for CD8+ population using the EasySep Human CD8+ T Cell Isolation Kit (Stemcell Technologies) and CD8+ cells were used for analysis.
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bioRxiv - Immunology 2023Quote: ... Tissue was collected in ImmunoCult-XF T cell Expansion Medium (immunoCult-XF; STEMCELL Technologies, Vancouver, BC, Canada) with 1% penicillin/streptomycin (P/S ...
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bioRxiv - Cell Biology 2024Quote: ... The T cells were cultured for 48 hours in ImmunoCult medium (STEMCELL Technologies Canada Inc., Vancouver, BC) for the control condition ...
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bioRxiv - Bioengineering 2024Quote: Primary human CD3+ T cells were isolated by negative selection from cryopreserved PBMCs (STEMCELL Tech, CAT#70025) using the EasySep Human T-cell Isolation Kit (STEMCELL Tech ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T-lymphocytes were supplemented with the following: TH1: 15 ng/mL IL-12 (StemCell Technologies #78028.1), 150 ng/µLIL-2 (StemCell Technologies #78081) ...
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bioRxiv - Immunology 2024Quote: ... PBMC from three individual donors were CD8+ T-cell depleted using Rosette Sep cocktail (StemCell Technologies Inc.), pooled ...
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bioRxiv - Bioengineering 2024Quote: Primary human T cells were isolated from fresh human peripheral blood leukopaks (cat. # 70500.2, Stem Cell Technologies) or buffy coats (cat ...
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bioRxiv - Biochemistry 2024Quote: ... primary CD8+ T cells were isolated from blood of anonymous donors by negative selection (STEMCELL Technologies, #17953). T cells were cultured in human T cell medium (HTCM ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were cryopreserved at 2 × 107 cells per ml in CryoStor CS10 cryopreservation medium (StemCell Technologies) until use ...
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bioRxiv - Biophysics 2023Quote: ... and the media further supplemented with 30 U mL-1 of human recombinant IL-2 protein (STEMCELL Technologies, USA). T cells were incubated in a humidified incubator with 5% CO2 at 37°C and were split to 0.5×106 cells mL-1 when their concentration surpassed 2.5×106 cells per well or when medium became depleted (turning yellow in color) ...
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bioRxiv - Cell Biology 2020Quote: ... Naïve CD4 T cells were isolated from CB mononuclear cells (CBMCs) obtained using Lymphoprep (StemCell Technologies Inc., Canada), followed by a first step of negative selection by magnetic bead separation using EasySep Human Naïve CD4 T cell isolation kit (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells and CD19+ B cells were negatively isolated using magnetic bead based separation (EasySep, StemCell Technologies). CD14+ monocytes were positively selected (EasySep ...
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bioRxiv - Bioengineering 2020Quote: ... as per the manufacturer’s instructions and were enriched for CD4+ or CD8+ T cells using RosetteSep (STEMCELL Technologies) T cell enrichment as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... counted and CD8+ or CD4+ T cells were magnetically separated with appropriate separation kits (Stemcell Technologies, Vancouver, Canada).