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Citations for Stemcell Technologies :
301 - 350 of 1526 citations for T complex protein 1 subunit epsilon CCT5 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Primary CD8+ and CD4+ T cells were isolated by negative selection through either RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) or RosetteSepTM Human CD4+ T Cell Enrichment Cocktail (StemCell technologies ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were immediately processed to isolate CD4+ T cells by negative selection using the EasySep Human CD4+ T Cell Isolation Cocktail (StemCell Technologies, Cat. #17952) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... CD8+ and CD3+ T cells were isolated from PBMCs using the EasySep™ Human CD8+ T Cell Isolation Kit and EasySep™ Human T Cell Isolation Kit (both from STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated from tumor-free wild type B6 mice using the mouse CD8 T cell isolation kit (STEMCELL Technologies cat #19853), labeled with Cell Trace Violet (Thermo Fisher cat #C34557 ...
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bioRxiv - Immunology 2022Quote: ... Splenic T cells were enriched using a magnetic-based commercial CD8 T cell negative selection kit (StemCell Technologies, BC, Canada; Supplemental Figure 3A) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... CD4+ T cells were isolated from frozen PBMC tubes using the EasySep™ Human CD4+ T Cell Isolation Kit (Stem Cell Technologies, #17952) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... splenic murine t cells were isolated from C57BL/6 mice using EasySep™ Mouse T Cell Isolation Kit (STEMCELL Technologies; Cat. No. 19851). Glioma cells were labeled with Cr51 (0.05 mCi ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T-cells were isolated from the single cell suspension using the EasySep Mouse CD8+ T-cell isolation kit (Stem Cell Technologies, 19853) and the EasyEights EasySep magnet (Stem Cell Technologies ...
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bioRxiv - Microbiology 2024Quote: ... human PBMCs (AllCells) were thawed and CD4+ T cells were isolated using the EasySep™ Human CD4+ T Cell Isolation Kit (Stem Cell Technologies). Naive CD4+ T cells were resuspended in complete RPMI (cRPMI ...
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bioRxiv - Immunology 2020Quote: ... all from BD Biosciences; anti-CD38-FITC (clone AT1; 2.5 μL; cat. # 60131FI) from STEMCELL Technologies; anti-CD161-BV421 (clone HP-3G10 ...
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bioRxiv - Synthetic Biology 2022Quote: Primary CD4+ and CD8+ T cells were isolated from blood of anonymous donors by negative selection using the Human CD4+ T cell isolation kit and Human CD8+ T cell isolation kit (STEMCELL Technologies #17952 and #17953). T cells were cryopreserved in RPMI1640 (UCSF cell culture core ...
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bioRxiv - Immunology 2023Quote: ... Purification of CD8+ T cells for sorting or cell culture was performed using a mouse CD8+ T cell isolation kit (Stem Cell Technologies, Cat# 19853), following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2022Quote: ... CD4+ T cells were isolated from the spleen of C57/BL6 mice using the CD4 T cell isolation kit from Stemcell Technologies (# 19852; Cologne, Germany) and subsequently naïve T helper cells (CD3+ ...
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bioRxiv - Immunology 2023Quote: Single cell suspensions of splenocytes were prepared and CD4+ T cells were isolated using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies; Cat # 19852A). CD4+ T cells were plated in a round bottom 96-well plate at 2 × 105 cells/well and activated using Mouse T Activator CD3/CD28 Dynabeads™ (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were isolated from healthy donor-derived PBMCs using the EasySep™ Human CD8+ T Cell Isolation Kit (STEMCELL Technologies, Cat. # 17953). CD8+ T-cells were then activated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: ... OT-I T cells (CD8+) were isolated from spleens of OT I mice using EasySep™ Mouse CD8+ T Cell Isolation Kit (Stem Cell Technologies, 19853A). Dendritic cells (DCs ...
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bioRxiv - Molecular Biology 2024Quote: ... Total CD4+ T cells were isolated by immunomagnetic negative selection using the EasySep™ Human CD4+ T Cell Isolation Kit (STEMCELL Technologies, cat. #17952) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... murine T cells were freshly isolated from NOD.Nr4a1GFP/Cre mice using the EasySep™ Mouse T Cell Isolation Kit (STEMCELL Technologies; Cat. No. 19851) per manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were allowed to recover in antibiotic-free medium containing 1 µg/mL DNase I solution (STEMCELL Technologies, Cambridge, MA) at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... (2) ImmunoCult™-XF T Cell Expansion Medium (STEMCELL Technologies) was supplemented with 1X Penicillin Streptomycin solution (ICM) ...
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bioRxiv - Cancer Biology 2020Quote: ... CD8+ T-cell isolation kit was from STEM cell technologies. Anti-PD1 (clone RMP1-14 ...
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bioRxiv - Microbiology 2020Quote: ... Immunocult human CD3/CD28 T cell activator (STEMCELL Technologies, USA) was used to stimulate the PBMCs as a positive control for immunological activation.
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bioRxiv - Bioengineering 2021Quote: ... Primary human peripheral blood CD8+ T cells (Stemcell Technologies - #70027) were expanded using fresh complete ImmunoCult™-XF T Cell Expansion Medium (Stemcell Technologies - #10981 ...
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bioRxiv - Immunology 2020Quote: EasySep separation kits (StemCell Technologies, T Cells: #19661, Neutrophils: #19257) were used to isolate CD3+ T cells or neutrophils directly from human whole blood ...
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bioRxiv - Immunology 2020Quote: ... Using a CD4+ T cell enrichment kit (StemCell Technologies, USA), CD4+ T cells were isolated from bulk splenocytes by negative selection ...
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bioRxiv - Immunology 2023Quote: ... or ImmunoCult Human CD3/CD28 T cell activator (Stemcell Technologies) was added at a dilution of 1:500.
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bioRxiv - Immunology 2023Quote: ... with EasySep Human CD4+ T Cell Isolation Kit (STEMCELL Technologies). Enriched CD4+ cells were stained for 30 min on ice with antibodies against anti-CD4 ...
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bioRxiv - Molecular Biology 2024Quote: ... Pan-T cells were obtained from StemCell Technologies (Catalog: 70024).
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Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... EasySep™ Human T-cell-negative isolation kit (StemCell Technologies) was used to isolate peripheral blood T cells from healthy donor leukopaks ...
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bioRxiv - Cancer Biology 2022Quote: ... Human T cells from anonymous donors sourced by STEMCELL Technologies were isolated by negative selection for CD3+ populations as per manufacturer’s instruction (EasySep ...
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bioRxiv - Bioengineering 2022Quote: Using StemSpan™ T Cell Generation Kit (STEMCELL Technologies, Inc.), CD34+ HSPCs were cultured in StemSpan™ SFEM II containing Lymphoid Progenitor Expansion Supplement on plates coated with Lymphoid Differentiation Coating Material for 14 days ...
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bioRxiv - Immunology 2023Quote: ... or EasySepTM Mouse T Cell Isolation Kit (#19851, StemCell Technologies) according to the manufacturer’s instructions and let to recover in cRPMI (10% FCS ...
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bioRxiv - Cancer Biology 2024Quote: Human T cells were isolated from a leukopak (Stemcell Technologies) using the Easy Sep Human T cell enrichment kit (Stemcell technologies) ...
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bioRxiv - Immunology 2024Quote: The EasySep Mouse CD8+ T Cell Isolation Kit (STEMCell technologies) was used to purify naïve OT-1 CD8+ T cells for proteomic analysis.
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bioRxiv - Immunology 2024Quote: ... the RosetteSep human CD4+ T cell enrichment cocktail (STEMCELL Technologies) was used prior to density gradient centrifugation ...
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bioRxiv - Immunology 2024Quote: ... T cells were maintained in Immunocult-XF media (STEMCELL Technologies) supplemented with 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... containing ImmunoCult™-XF T Cell Expansion Medium (StemCell Technologies) and 50 U/mL Penicillin-Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... in ImmunoCult-XF T Cell Exp Medium (Stemcell Technologies, 10981) supplemented with 300IU/ml recombinant human IL-2 (Stemcell Technologies ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... splenic T-lymphocytes were negatively selected from total splenocytes through use of EasySep Mouse T-cell Isolation Kit (Stem Cell Technologies #19851, Vancouver, BC, Canada). Samples were validated for >90% viability and purity before subsequent use.
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bioRxiv - Immunology 2021Quote: ... Memory CD4+ or CD8+ T cells were either isolated from PBMCs by EasySep Human Memory CD4+ or CD8+ T cell enrichment kit (STEMCELL Technologies, Cat#: 19157 or 19159), or isolated from purified CD4+ or CD8+ T cells by CD45RO microbeads (see Suppl ...
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bioRxiv - Immunology 2021Quote: Untouched total T or isolated CD4+ or CD8+ T cells were purified from buffy coats or whole blood with RosetteSep Human T or CD4+ or CD8+ T cell enrichment cocktails (STEMCELL Technologies, Cat#: 15061, 15062, 15063). Memory CD4+ or CD8+ T cells were either isolated from PBMCs by EasySep Human Memory CD4+ or CD8+ T cell enrichment kit (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... PBMC samples were thawed on the day of activation and T cells were purified via the EasySep™ Human CD8+ T Cell Enrichment Kit (StemCell Technologies Inc, cat#19053). Isolated T cells were seeded in the CD3/CD28 precoated well plates at a concentration of 500,000 cells/mL with 1 mL/well in ImmunoCult™-XF T Cell Expansion Medium (StemCell Technologies Inc ...
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bioRxiv - Immunology 2024Quote: T cells were extracted from OT-II mouse spleens and magnetically sorted using a T-cell extraction kit from Stem Cell Technologies (catalog number 19851). Cells were incubated in culture media (RPMI+ 10% HIFBS + Pen/Strep + 1 mM HEPES + 50 μM beta mercaptoethanol ...
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bioRxiv - Cell Biology 2024Quote: ... The antibodies used are anti-TRA-1-60-AF488 (STEMCELL Technologies, 60064AD.1), anti-CXCR4-PE (BioLegend ...
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bioRxiv - Immunology 2020Quote: Primary CD4+ T-cells for in-vitro HIV-1 infection were isolated from total blood of healthy individuals using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies Inc. ...
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bioRxiv - Pathology 2023Quote: ... CD4+ T cells were isolated from 40 ml of peripheral blood from HIV-1-infected individuals on ART by immunodensity negative-selection using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies). Cells were plated at a concentration of 1-3×106/ml in RF10 and stimulated with 2 ng/ml PMA for 16 h when indicated ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were isolated from lymph nodes of C57BL/6 Rag1-/+ OT-1-/+ mice and purified using the EasySep™ mouse CD8+ T cell isolation kit (STEMCELL Technologies) by negative selection ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then resuspended at 1 million/mL in either Immunocult-XF T-cell Expansion Medium (Cat # 10981, STEMCELL Technologies, Vancouver, Canada) or TEXMACs Cell Culture Medium (Cat # 130-097-196 ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... CCR6low and CCR6high memory subgroups and cultured at 1 × 106 cells/ml in 24 well plates in ImmunoCult XF T cell expansion medium (Stem Cell Technologies) supplemented with 10% FBS and IL-2 (200 units/ml) ...
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bioRxiv - Genomics 2024Quote: ... on Day -1 CD4+ T cells are isolated from blood (300 ml) of HIV seronegative donors by negative selection (StemCell Technologies, Inc). On Day 0 ...