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Citations for Stemcell Technologies :
701 - 750 of 1526 citations for T complex protein 1 subunit epsilon CCT5 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: 1 × 105 memory CD4+ T cells freshly purified from PBMC by negative selection and magnetic separetion(EasySep™ Human memory CD4+ T cell enrichment kit, STEMCELL Technologies) were cocultured at 1:1 ratio for 6 days with anti-CD3/CD28–coated beads (Dynabeads ...
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bioRxiv - Immunology 2020Quote: ... C57BL/6 mice by use of magnetic separation with the EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: T lymphocytes were isolated from the spleen of C57BL/6J mice using a negative selection isolation kit (EasySep™ Mouse T Cell Isolation Kit, StemCell Technologies). Cells were plated in a 48 wells plate (Corning ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were isolated from SPLNs of 5 donors from each group using a mouse CD4+ T cell isolation kit following manufacturer’s instructions (STEMCELL Technologies, Vancouver, BC). CD4+ T cells from NT and CPZ donors were injected intraperitoneally into RAG1 KO recipient mice ...
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bioRxiv - Immunology 2021Quote: ... CD4+ CD62L+ naïve T cells were isolated from 8-12 week-old mice using CD4 negative enrichment kits (Stemcell Technologies, Vancouver, Canada) and CD62L microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... T cells at a concentration of 2e6/ml were re-stimulated with ImmunoCult™ Human CD3/CD28/CD2 (Stemcell Technologies cat 10970) at 6.25μl/ml ...
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12-Lipoxygenase Governs the Innate Immune Pathogenesis of Islet Inflammation and Autoimmune DiabetesbioRxiv - Immunology 2021Quote: ... Naïve T cells were isolated and purified from spleen and lymph nodes of C57BL/6J mice using EasySep™ Mouse CD4+ T Cell Isolation Kit (Stemcell Technologies).
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bioRxiv - Bioengineering 2022Quote: ... via Ficoll-Paque (Cytavia) gradient selection for peripheral blood mono-nuclear cells followed by human CD8+ T cell negative magnetic selection (Stem Cell Technologies). Primary CD8+ T cells were thawed in supplemented RPMI 1640 2-12 hours prior to the start of experiments.
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bioRxiv - Cancer Biology 2022Quote: ... Naïve CD8+ T cells were harvested from CD1 wildtype P25 mice and processed using the EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit (Stemcell Technologies, 19858). Following isolation ...
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bioRxiv - Immunology 2024Quote: ... splenic P14 TM were enriched at indicated time points after LCMV Arm by removal of APC- or PE-labeled CD4+ T and CD19+ B cells with anti-APC/PE magnetic microbeads (Miltenyi Biotec & STEMCELL Technologies) and 5×103 P14 TM were transferred i.v ...
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bioRxiv - Bioengineering 2024Quote: ... CD3+ primary human T-cells were isolated by negative selection as per manufacturer’s instructions (Cat # 15021, 15061, RosetteSep Human T-cell Enrichment Cocktail, STEMCELL Technologies, Vancouver, Canada). T-cell pellets were resuspended in dilution medium and counted using a hemocytometer with 0.4% Trypan blue viability stain (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... T cells from the transgenic mouse splenocytes were enriched by magnetic isolation using the EasySepTM Mouse T Cell Isolation Kit (Stem Cell Technologies). T cells were centrifuged and resuspended in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... After an additional 48 hours non-adhered cells were discarded and adhered T-ALL cells were separated from glioblastoma cells using EasySep Human CD3 positive Selection kit II (Stem Cell Technologies) and viability was determined by trypan blue staining (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were subsequently isolated from the PBMC population by immunomagnetic negative selective with the EasySep Human T Cell Isolation kit (StemCell Technologies, Canada). Isolated T cells with >95% viability and >95% purity were either cryopreserved or directly cultured for subsequent experiments ...
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bioRxiv - Immunology 2023Quote: ... Human CD4+ T cells were isolated from approximately 2 x 108 elutriated lymphocytes to approximately 90% purity by negative selection using RosetteSep human CD4+ T cell enrichment cocktail (15062; Stem Cell Technologies), using Ficoll/Hypaque (45-001-749 ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T lymphocytes were then purified from resting PBMCs by negative selection using immunomagnetic beads per the manufacturer’s instructions (StemCell Technologies, Vancouver, BC). CD4+ T lymphocytes were activated with phytohemagglutinin-L (10 µg/mL ...
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bioRxiv - Immunology 2023Quote: CD63+ DCs or CD63- control DCs isolated from the LNs of Balb/c mice were sorted by FACS as described previously and cultured with naïve CD4+ T cells magnetically isolated from Fox3-GFP+ mice (C57Bl/6 background) using EasySep Mouse Naïve Cd4+ T cell isolation kit (Stemcell Technologies, Cat# 19765) at a ratio of 1:5 DC:T cell for 5 days in RPMI containing 10% FBS ...
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bioRxiv - Genomics 2023Quote: 50 U/mL IL-2 (Amerisource Bergen, #10101641) and stimulated with 6.25 uL/mL ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies, #10990). Following activation and electroporation ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from pooled splenic cell suspensions by magnetic negative selection using EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells that passed through 70-uM cell strainer were purified with EasySepTM Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies Inc). For T-cell proliferation assays ...
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bioRxiv - Cancer Biology 2023Quote: ... Naïve CD8+ T-cells were isolated from the thawed CD14-population on Day 6 using the EasySep Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies Cat. #19258) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads per the manufacturer’s instructions (StemCell Technologies, Vancouver, BC) and were activated with phytohemagglutinin-L (10 µg/mL ...
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bioRxiv - Immunology 2024Quote: Primary human CD8+ T lymphocytes were isolated from buffy coats of anonymous healthy donors and purified (>95% purity) by negative selection using the RosetteSep™ Human CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies). Buffy coats were collected at the Siena University Hospital after obtaining written informed consent from each donor and processed following standard ethical procedures outlined in the Declaration of Helsinki ...
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bioRxiv - Immunology 2024Quote: A minimum of 5×104 cells were kept for mononuclear cell lysis from PBMC and SFMC samples before CD4+ T cells enrichment via magnetic negative selection (EasySep™, StemCell Technologies). Tregs were then sorted from CD4+ T cells on live (FVD- ...
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bioRxiv - Microbiology 2024Quote: ... Primary CD4+ T cells were purified from resting PBMCs by negative selection using immunomagnetic beads per the manufacturer’s instructions (StemCell Technologies, Vancouver, BC) and were activated with phytohemagglutinin-L (PHA-L ...
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bioRxiv - Biochemistry 2024Quote: ... CD4+ and CD8+ T cells were then purified by negative selection and magnetic separation (Stem Cell Technologies Cat# 17952 and Cat# 17953). Purified human CD4+ and CD8+ T cells were activated by plate-bound anti-human CD3 (2 µg/mL ...
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bioRxiv - Immunology 2024Quote: ... Remaining unstained cells was restimulated with 12.5 uL/mL Immunocult Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies, catalog no. 10970). Forty-eight hours post-restimulation ...
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bioRxiv - Synthetic Biology 2024Quote: ... 12053231) and CD4+ and CD8+ T cells were isolated using a magnetic bead-based negative selection kit (easySEP, Stem Cell technology, 17951) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 10 x 106 PBMCs were enriched for B cells using CD3 T cell depletion via the EasySep™ Human CD3 Positive Selection Kit II (StemCell Technologies) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... cones obtained from the Oklahoma Blood Institute (Oklahoma City, OK) using negative selection with the EasySep™ Human T Cell Isolation Kit (cat. no. 17951; STEMCELL Technologies). Alternatively ...
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bioRxiv - Immunology 2024Quote: ... Mario-CAR T cells were selected using the EasySep™ Human PE Positive Selection Kit II (Stem Cell Technologies, Cat-no. 17654) with PE NGFR antibody (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... the mice were irradiated (350 rads) and injected intravenously with CD3+ T cell-depleted cord blood (EasySep Human CD3 Positive Selection Kit, 18051, Stem Cell Technologies) containing approximately 105 human CD34+ hematopoietic stem cells (HSC ...
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bioRxiv - Immunology 2024Quote: ... Mice received 1x106 CAR+ or an equivalent number of non-engineered T cells in 200 µL of CryoStor® CS10 cryopreservation medium (StemCell Technologies) intravenously ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were enriched from peripheral blood of HLA-A2− subjects using the Rosettesep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies) and CD25+ cells were separated using CD25 MicroBeads II (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2024Quote: ... BT-002-010) once a week and 7ul/mL ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stem Cell Technologies, 10970) every other week ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse CAR-19 cells were generated first by isolating T cells from spleens of BALB/c mice followed by CD3 negative selection (Stemcell Technologies #19851A). T cells were activated and transduced with m1928z-mCherry vector as previously described (45).
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bioRxiv - Cancer Biology 2024Quote: Tumor-infiltrating lymphocytes were purified from dissociated MC38-derived tumors using the EasySep Mouse T Cell Isolation Kit (STEMCELL Technologies, Vancouver, Canada), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Mouse naïve CD4 T and B cells were isolated using mouse CD4 and B cell negative selection kits (Stemcell Technologies, Cambridge, MA). CD4+Foxp3GFP+ Tregs or CD4+Foxp3 GFP− naïve CD4 T cells were sorted from total CD4 T cells from LNs and spleens of WT or LTα−/− Foxp3-GFP mice using a FACS Aria II (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with a cocktail of lineage specific antibodies (CD5, CD11b, CD19, B220, Gr-1 and TER119, Stemcell Technologies) for negative selection of Lin-cells using an EasyStep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then incubated overnight at 4°C with primary antibody (beta-tubulin III 1:500; STEMCELL Technologies 60052) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 1.5 nM bone morphogenetic protein 4 (BMP4, Stemcell technologies) was added to the medium for another three days ...
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bioRxiv - Cancer Biology 2021Quote: Human primary total T cells (CD4 and CD8) were isolated from normal healthy donors following leukapheresis by negative selection using RosetteSep kits (Stemcell Technologies, Vancouver, CA). All specimens were collected with protocol approved by University Review Board and written informed consent was obtained from each donor ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were purified from peripheral blood samples by the negative selection EasySepTM Human T cell Enrichment Kit (Catalog #17951, StemCell Technologies, Vancouver, Canada). Enriched T cells were stained with Cell Proliferation Dye eFluorTM 450 (Catalog #65-0842 ...
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bioRxiv - Bioengineering 2021Quote: ... After a 24 hr culture out of thaw the PBMCs were activated with ImmunoCult™ human CD3/CD28 T cell activator reagent from STEMCELL Technologies (#10971) for 3 days according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Preparation of cells: Human CD4+ cells were isolated from buffy coats using EasySep™ Human CD4+ T Cell Isolation Kit (Stemcell Tech, # 17952), resuspended with complete RPMI (cRPMI ...
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bioRxiv - Bioengineering 2021Quote: ... CD3+ T cells or CD56+CD3-NK cells were isolated from the PBMC population using the EasySep Human T Cell Isolation Kit or EasySep Human NK Cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: Primary human CD8+ T cells were isolated from buffy coats of healthy donors and purified (>95%) by negative selection using the RosetteSep™ Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies, #15063), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from spleens of WT C57BL/6 mice by negative selection using the EasySep™ Mouse CD8+ T cell isolation kit (Stem Cell Technologies) according to the manufacturer’s protocol and cultured in complete media ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from spleen and peripheral lymph nodes from indicated mice by negative selection using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies Inc). 1.75 x 105 cells were cultured for 4 days in RPMI1640 supplemented with 10% heat-inactivated FCS ...