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Citations for Stemcell Technologies :
651 - 700 of 1526 citations for T complex protein 1 subunit epsilon CCT5 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Human T cells were purified (> 96% purity) by magnetic negative selection using an EasySep Human T Cell Isolation Kit (Cat#17951, Stemcell Technologies) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: CD4+ cells were isolated from freshly dissociated mouse spleen using the EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, 19852). CD4+ cells were then immediately used for bulk ATAC-seq ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated from thawed PBMCs by negative magnetic selection using the EasySep Human CD4 T Cell Enrichment Kit (STEMCELL Technologies) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... human and murine PBMCs underwent pre-activation through culturing in PBMCs culture medium supplemented with ImmunoCultTM Human CD3/CD28 T cell Activator (StemCell Technologies) and suspension in LymphoGrow II medium (Cytogen) ...
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were isolated from pooled splenic suspensions by magnetic negative selection using EasySep Mouse CD4+ T Cell Isolation Kit (StemCell Technologies) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were generated and enriched for CD4+ T cells by negative selection using EasySep magnetic bead kit (STEMCELL Technologies) and stained with anti-mouse CD4 (RM4-5 ...
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bioRxiv - Cell Biology 2023Quote: Primary human CD4+ and CD8+ T cells were isolated from commercially available whole human blood (STEMCELL Technologies Inc., USA, Cat# 70507.1) with EasySep Human CD4+ or CD8+ T Cell Isolation Kits (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2023Quote: ... Human CD8+ T cells were isolated by negative selection using the EasySep Human CD8 negative isolation kit (StemCell Technologies, Vancouver, BC).
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bioRxiv - Immunology 2024Quote: ... CD8+ T-cells were isolated from spleens of P14 donor mice by negative selection using the EasySepTM Mouse CD8+ T-cell isolation kit (STEMCELL Technologies). P14 cells were activated in vitro for 24-28 hours with 100U/mL recombinant IL-2 ...
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bioRxiv - Cell Biology 2024Quote: The CD4+ T-cell subpopulation was isolated from the CD14- fraction of PBMCs using the Easysep human CD4+ T-cell isolation kit (Stemcell Technology) as per the instructions of the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... ETRAMP-specific CD4+ T cells were enriched using APC- and PE-conjugated tetramers and EasySep APC and PE Positive Selection Kits (STEMCELL Technologies). Cells were then stained with fluorescent dye-conjugated antibodies (see Table I ...
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bioRxiv - Cancer Biology 2023Quote: CD8 T cells were isolated from the lymph node of naïve mice with unactivated Confetti cassette using EasySep™ Mouse CD8+ T Cell Isolation Kit (StemCell Technologies) and macrophages were sorted from the tumors via FACS sorting ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from peripheral lymph nodes (inguinal, axillary, brachial and superficial cervical) by negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (StemCell Technologies). CD4+ T cells were stimulated in 96 well flat-bottomed plates coated with 20mg/ml αCD3 (clone 145-2C11 ...
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bioRxiv - Immunology 2024Quote: ... CD4 T cells were isolated from the spleens of young (2- to 3-month-old) mice spleen using a magnetic microbeads negative selection kit (EasySep Mouse CD4 T Cell Isolation Kit; STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells from spleens were negatively enriched using an EasySep™ Mouse CD8+ T Cell Isolation Kit (#19853) according to the manufacturer’s instructions (STEMCELL Technologies). Enriched CD8+ T cells (92% - 96% purity ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells were isolated from spleens and lymph nodes of P14 Tcf7GFP or OTI Tcf7GFP mice using the EasySepTM Mouse CD8+ T cell Isolation Kit (Stemcell Technologies), following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: Human CD3+ T cells were obtained from buffy coats of healthy donors by negative selection using RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies) and stored at -80ºC until use ...
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bioRxiv - Immunology 2022Quote: ... 12 day-expanded cells were further expanded for 7 days with ImmunoCult Human CD3/CD28/CD2 T Cell Activator (25 μl/ml; STEMCELL Technologies), then rested overnight with reduced IL-2 as above (total 20-day culture) ...
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bioRxiv - Immunology 2022Quote: ... and B and T cells were isolated using EasySep Negative selection Mouse B and T cell Enrichment Kits according to the manufacturers protocol (StemCell Technologies). Cells were then suspended in transfer buffer (10 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... We used a method of MAIT cell expansion as previously described13 with the following modifications: instead of using base medium of Immunocult-XF T cell expansion medium (Stemcell Technologies) we used RPMI-1640 and substituted CTS serum (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... and purified CD4+ T cells were obtained by positive selection with the “EasySep Mouse CD4 Positive Selection II” kit (StemCell Technologies). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse T-cells were isolated from mouse PP/lymph nodes/spleens and tumors using EasySep Mouse CD8+ T Cell Isolation kit (StemCell Technologies). For FACS analysis ...
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bioRxiv - Genomics 2023Quote: Human CD8+ T cells were obtained from either pooled PBMC donors (ZenBio) using negative selection human CD8 isolation kits (StemCell Technologies) or directly from vials containing isolated CD8+ T cells from individual donors (StemCell Technologies) ...
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bioRxiv - Bioengineering 2023Quote: Human CD8+ T cells were purified from buffy coats from healthy donors at the NIH Blood Bank using EasySepTM Human CD8+ T Cell Isolation Kit (StemCell Technologies). Approximately 2×104 CD8+ T cells were plated in each well of a 96-well plate immediately following isolation ...
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bioRxiv - Immunology 2024Quote: ... OT-I+/- or total CD8+ T cells were purified from spleen and peripheral lymph nodes by negative selection using the EasySepTM Mouse CD8+ T cell isolation kit (#19753, StemCell Technologies). For in vitro experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then stimulated with 12 μL/mL ImmunoCult™(TM) Human CD3/CD28 T Cell Activator (STEMCELL Technologies, cat. #10971).
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bioRxiv - Microbiology 2024Quote: ... 00196979 for the CF donor) and cultured in T-25 flasks using PneumaCult–Ex Plus medium (P-Ex Plus) (Stemcell Technologies). Unless mentioned otherwise ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells (OT-I cells) were isolated by negative selection with the EasySepTM Mouse CD8 T cell isolation kit (Catalog #19853, Stemcell Technologies) following the manufacturers protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... EB-HSPCs, and primary mPBSCs from a healthy donor (female, 30 years) were differentiated using the StemDiff T Cell Kit (STEMCELL Technologies) with minor modifications to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... CD4+ T lymphocytes were isolated from PBMCs by positive selection using magnetic beads (EasySep Human CD4 positive selection kit II, StemCell Technologies) and activated using ImmunoCult human CD3/CD28 T cell activator cocktail (StemCell Technologies ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T were isolated from the spleen and LN of OT-II TCR Tg GFP+ mice using the Easysep Mouse CD4+ T cell isolation kit (Stemcell Technologies) or MojoSort Mouse CD4 Naïve T cell isolation kit (BioLegend ...
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bioRxiv - Immunology 2024Quote: Spleen and/or lymph node (LN) cells were purified by negative selection for total or naïve CD8+ or CD4+ T cells (Stem Cell Technologies). Bulk splenocytes or purified naïve T cells were plated with stimulatory anti-CD3ε and anti-CD28 antibodies with or without anti–IL-2 blocking antibody at indicated doses ...
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bioRxiv - Immunology 2024Quote: ... were irradiated with 2 Gy by 177Lu diluted in 3 mL of ImmunoCult-XF T cell Expansion Medium (cat. no. 10981; STEMCELL Technologies) supplemented with 50 IU/mL of IL-2 over 3 days and then examined by flow cytometry for viability as previously described 25.
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bioRxiv - Immunology 2024Quote: ... T cells were isolated from peripheral blood of healthy donors under a University of Wisconsin-Madison IRB-approved protocol (2017-1070) using the RosetteSep Human T Cell Enrichment Cocktail (cat. no. 15021, 15061; STEMCELL Technologies). The sequence for a third generation GD2-CD28-OX40-CD3ζ construct was generously provided by Malcolm Brenner (Baylor College of Medicine) ...
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bioRxiv - Immunology 2024Quote: ... γδ TCR+ cells were enriched by immunomagnetic negative selection using an EasySep Human Gamma/Delta T Cell Isolation Kit (STEMCELL Technologies). After purification ...
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bioRxiv - Immunology 2024Quote: ... at the final concentration of 0.3×106 cells/ml and stimulated with ImmunoCult™ Human CD2/CD3/CD28 T Cell Activator (Stemcell Technologies). In the next step ...
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bioRxiv - Microbiology 2024Quote: CD4+ T-cells were isolated from frozen PBMCs from 5 study participants using the EasySep Human CD4+ T-Cell Enrichment Kit (Stemcell Technologies). 2 million CD4+ T-cells were then cultured in 96-well plates in duplicate in 200 μL R10+ media supplemented with 100 U/mL IL-2 ...
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bioRxiv - Cancer Biology 2024Quote: CD8+ T cells were isolated from the mouse spleen and injected with mEERL cells with scramble or Marchf8 sgRNA using the CD8+ T-cell Isolation Kit (STEMCELL Technologies). The cells were labeled with 3 µM CFSE (Invitrogen) ...
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bioRxiv - Bioengineering 2024Quote: ... PBMCs were separated by gradient centrifugation by using Lymphoprep (Axonlab) and CD8+ T cells were further negatively selected by using the easySEP (Stemcell Technologies) magnetic bead isolation kit following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: CD8+ T cells were isolated from the spleens of C57BL/6J or mice with magnetic bead-based CD8 T cell isolation kit (Cat # 19853, StemCell Technologies). T cells from naïve mice were stained with 5µM Cell Trace Violet (Cat# C34557 ...
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bioRxiv - Bioengineering 2024Quote: ... CD8+/CD4+T cells (for OT-I/OT-II mice) were isolated from this suspension using the EasySep Mouse CD8+/CD4+ T Cell Isolation kit (STEMCELL Technologies; Cat#19852 for CD4+T ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a first step of negative selection by magnetic bead separation using EasySep Human Naïve CD4 T cell isolation kit (STEMCELL Technologies Inc., Canada) and FACS on a BD FACSAriaTM II using the following markers/staining ...
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bioRxiv - Immunology 2021Quote: OVA (SIINFEKL)-specific CD8+ T cells were isolated from the spleen and lymph nodes of healthy 6-8-week old OT-I mice using EasySep™ Mouse CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: Murine T-cells were isolated from spleens of healthy 6-8-week old C57BL/6 mice using EasySep™ Mouse CD4+ or CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2022Quote: ... Primary human MSC were sorted for CD317 expression and co-cultured with commercially sourced cryopreserved CD4+ human cord blood T cells (Stem Cell Technologies).
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bioRxiv - Immunology 2021Quote: ... C57BL/6.OT-I.Ubiquitin-GFP CD8+ T cells were enriched from single-cell suspensions of lymph nodes and spleens using mouse CD8-negative selection kits (Stem Cell Technologies). Enriched CD8 T cells (5×106 ...
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bioRxiv - Microbiology 2021Quote: ... and CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads per the manufacturer’s instructions (StemCell Technologies, Vancouver, BC) and were activated with phytohemagglutinin-L (10 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... the Negative Selection EasySep CD4+ T cell kit (17951) or RosetteSep Human CD4+ Cell Isolation Kit (15062) from Stemcell Technologies (Vancouver, Canada) according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2022Quote: ... CAR T cells were positively selected for truncated CD19 using the EasySep™ CD19 Positive Enrichment Kit I or II (StemCell Technologies) (for PSCA-CAR T cells ...
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bioRxiv - Immunology 2020Quote: ... Primary CD4+ T cells were isolated from peripheral blood mononuclear cells (PBMCs) and enriched via negative selection with an EasySep Human CD4+ T Cell Isolation Kit (Stemcell Technologies, Canada). Primary CD4+ T cells were cultured at 37°C in RPMI supplemented with 10% FBS ...