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Citations for Stemcell Technologies :
601 - 650 of 1526 citations for T complex protein 1 subunit epsilon CCT5 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... CD8+ T cells were isolated from PBMCs by positive magnetic selection using EasySep Human CD8 Positive Selection Kit (StemCell Technologies). CD4+ T cells were isolated from PBMCs by negative magnetic selection using EasySep Human CD4 T cell Isolation Kit (StemCell Technologies) ...
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bioRxiv - Biochemistry 2020Quote: CD8+ or total T cells were isolated from spleens with an EasySep(tm) T Cell Isolation Kit according to the manufacturer’s instructions (STEMCELL Technologies).
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bioRxiv - Bioengineering 2021Quote: ... the cuvettes were allowed to rest for 15 minutes before the T cells were gently moved to antibiotic-free medium containing 1µg/mL DNase (StemCell Tech) at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Naive human CD4+ T cells were isolated using the EasySep Human Naïve CD4 TCell Iso Kit II (Stem Cell Technologies) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... or total T cells using EasySep isolation kits (Catalog Nos. 19765, 19853, and 19851; STEMCELL Technologies Inc., Vancouver, BC, Canada). Negative selection was confirmed by flow cytometry analysis to > 90% purity ...
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bioRxiv - Immunology 2021Quote: Naïve CD8 T cells were isolated from young female (6w-8w) B6 mouse lymph nodes and spleens with the EasySep Mouse Naïve CD8 T Cell Isolation Kit (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads per the manufacturer’s instructions (StemCell Technologies) and were activated with phytohemagglutinin-L (10 μg/mL ...
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bioRxiv - Immunology 2020Quote: Total CD4 T cells from donor mice were purified from spleens using STEMCELL EasySep CD4 T Cell Isolation Kit according to manufacturer’s instruction (STEMCELL Technologies). Depending on individual experiment ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads according to the manufacturer’s instructions (StemCell Technologies). The cells were activated with phytohemagglutinin-L (10 μg/mL ...
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bioRxiv - Immunology 2023Quote: OVA-specific CD8+ T cells were isolated from the spleens and lymph nodes of OTI/Thy1.1 mice by negative selection (Stemcell Technologies) and labeled with CFSE for proliferation tracking ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were enriched for CD4+ cells using either the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies), or MojoSortTM CD4 T cell isolation kit (BioLegend) ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by sorting of cells using the SONY SH800 sorter or obtained with the naïve human CD4+ T cell isolation kit (StemCell Technology). For T helper cell culture ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were purified from spleen and peripheral lymph nodes by negative selection (StemCell Technologies, Vancouver, BC, Canada). Isolated naïve CD8+ cells (1 x 106 cells/mL ...
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bioRxiv - Immunology 2023Quote: Naïve CD4 T cells were isolated from the pooled spleens and LNs of 4 week old Thy1.1 expressing C57BL/6J mice using the EasySep Mouse Naive CD4 T cell Isolation Kit (StemCell Technologies). Isolated cells were stained with eFluor 450 viability dye (eBiosciences ...
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bioRxiv - Immunology 2024Quote: ... Tregs were seeded at an approximate density of 1.2 million cells/cm2 in ImmunoCult-XF T cell expansion medium (STEMCELL Technologies) supplemented with 50 μM β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were either left unactivated or activated with Immunocult Human CD3/CD28/CD2 T cell activator per manufacturer’s instructions (StemCell Technologies) for 24 or 48hrs ...
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bioRxiv - Immunology 2022Quote: ... lymph nodes and spleen from 16-24-week-old female or male C57Bl/6-Foxp3gfp × Thy1.1 mice were collected and CD4+ T cells isolated by negative selection (STEMCELL Technologies). Tregs were sorted as live CD4+CD8-Thy1.1+Foxp3gfp+ using a MoFlow® Astrios (Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: CD4+ T naïve T cells from the spleens of WT mice were isolated by negative selection using the EasySep Mouse Naïve CD4+ T cell Isolation kit (StemCell Technologies). T cells were activated with 1 μg/ml plate-bound anti-CD3 (BioLegend ...
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bioRxiv - Molecular Biology 2023Quote: Naïve CD4+ T cells from spleen and lymph nodes were enriched by negative selection using EasySepTM Mouse Naïve CD4+ T cell isolation kit (STEMCELL Technologies). 100,000 cells/well (50,000 CD45.1- cells and 50,000 CD45.1+ cells ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were enriched from spleen and lymph nodes of CD45.1+ mice by negative selection using EasySepTM Mouse CD4+ T cell isolation kit (STEMCELL Technologies), and FACS sorted as CD4+CD25- CD44loCD62Lhi cells ...
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bioRxiv - Immunology 2022Quote: ... and used directly for total CD4+ T cell enrichment by negative magnetic selection using Easysep magnetic separation kits (STEMCELL Technologies). Cell suspension was stained with anti-CD4 (RPA-T4) ...
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bioRxiv - Immunology 2024Quote: ... lymph nodes and spleen from female B6-Foxp3gfp Thy1.1 mice were collected and CD4+ T cells were isolated by negative selection (STEMCELL Technologies). Tregs were sorted as live CD4+CD8–Thy1.1+Foxp3gfp+ or CD4+CD8–Thy1.1+CD25+CD62L+ cells using a Moflo Astrios cell sorter (Beckman Coulter) ...
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bioRxiv - Immunology 2024Quote: CD8 T cells were purified from spleens of CD45.1 infected mice using the EasySep Mouse CD8 T cell isolation kit (Stemcell Technologies). The enriched CD8 cell suspension was then labeled with fluorescent antibodies (CD8 ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were isolated from peripheral blood of healthy donors with Human T Cell Enrichment Kit (Stem Cell Technology #19051). Human T cells were cultured in RPMI-1640 medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: Naïve CD4+ or CD8+ T cells were isolated from mouse spleen and lymph nodes by using a mouse naïve CD4+ or CD8+ T cell isolation kit (STEMCELL Technologies). Purified naïve CD4+ or CD8+ T cells were labeled with CellTrace Violet (Invitrogen ...
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bioRxiv - Immunology 2024Quote: Naïve CD4+ T cells were purified from mouse spleen and lymph nodes by using a mouse naïve CD4+ T cell isolation kit (STEMCELL Technologies) and polarized following the manufacturer’s protocol for Immunocult Mouse Th1 or Th2 differentiation supplements (STEMCELL Technologies) ...
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bioRxiv - Bioengineering 2024Quote: ... Bavarian Red Cross) obtained from healthy and anonymous donors using EasySep T Cell Isolation Kit (cat. # 17951, Stem Cell Technologies). For CAR-T cell generation ...
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bioRxiv - Bioengineering 2020Quote: ... and plated onto Matrigel®-coated six-well culture plates in mTeSR™1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y27632 (ROCK inhibitor; Stem Cell Technologies) at a density between 25,000 and 50,000 cells cm-2 ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells were isolated from peripheral blood of donor 2395 via negative selection using the RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primary human CD4+ T cells were isolated from anonymous donor blood after apheresis by negative selection (STEMCELL Technologies #15062 and 15023). T cells were cryopreserved in RPMI-1640 (Corning #10-040-CV ...
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bioRxiv - Immunology 2021Quote: ... primary naïve CD4+ T cells were isolated from the spleen of OT-II mice (B6.Cg-Tg(TcraTcrb)425Cbn/J) using EasySep Mouse CD4+ T cell isolation kit (Stemcell Technologies) after homogenization with a 70 µm cell strainer and resuspending the cells in PBS supplemented with 2 % FCS and 1 mM EDTA ...
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bioRxiv - Synthetic Biology 2021Quote: Primary CD4+ and CD8+ T cells were isolated from anonymous donor blood after apheresis by negative selection (STEMCELL Technologies #15062 & 15063). Blood was obtained from Blood Centers of the Pacific (San Francisco ...
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bioRxiv - Bioengineering 2022Quote: T cells were isolated from human donor whole blood samples by negative selection using the RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies). Isolated T cells were seeded in a fresh complete ImmunoCult™-XF T cell expansion medium (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2022Quote: CD8+ T-cells were aseptically isolated from the spleen and lymph nodes of C57BL/6 or FVB (6-8 weeks old) mice using the negative selection Easysep mouse CD8+ T-cell isolation kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: CD8+ T cells were isolated from the spleens or mesenteric lymph nodes (MLNs) using the EasySep Mouse CD8+ T Cell Isolation Kit (StemCell Technologies). Where indicated ...
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bioRxiv - Immunology 2021Quote: Primary human CD4+ T cells were isolated from PBMCs using a Human resting CD4+ T Cells Isolation Kit I according to the manufacturer instructions (STEMCELL Technologies). Briefly ...
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bioRxiv - Synthetic Biology 2020Quote: ... Regulatory T cells were isolated using the EasySep human CD4+CD127lowCD25+ regulatory T cell isolation kit according to manufacturer’s protocol (STEMCELL Technologies, 18063). Primary T cells were cultured in X-Vivo 15 media (Lonza ...
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bioRxiv - Immunology 2020Quote: ... Resting CD4+ T cells (rCD4s) were isolated from PBMC by magnetic negative selection using the EasySep Human Resting CD4+ T cell Isolation Kit (StemCell Technologies). In some experiments ...
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bioRxiv - Genomics 2021Quote: ... CD8+ T cells were purified from thawed PBMCs by negative selection using EasySep Human CD8+ T Cell Enrichment Kit (Cat#19053, STEMCELL Technologies) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: Aliquots of fresh blood or frozen ficoll-separated peripheral blood cells were fractionated using EasySep Human Myeloid Positive Selection and EasySep Human T Cell Enrichment kits (StemCell Technologies. DNA was extracted from the fractionated subsets using MagMAX DNA Multi-Sample Ultra 2.0 Kit (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... were isolated from perioperative human patients’ brain tissues (Nervous System Tumor Bank) using EasySep Human CD8 T Cells Isolation Kit (STEMCELL Technologies) following the tissue digestion as previously described [68 ...
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bioRxiv - Immunology 2021Quote: Memory and effector CD4 T cells were purified from total splenocytes isolated from immunized mice using EasySepTM Mouse Memory CD4+ T Cell Isolation Kit (StemCell Technologies) as per manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... followed by enrichment of either effector/memory T cells (Human Memory CD4 T cell Enrichment kit; EasySep) or Treg cells (Human CD4+CD127loCD49d− Treg Enrichment Kit; EasySep) (all from StemCell Technologies).
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bioRxiv - Immunology 2021Quote: ... Spleens of recipient mice were collected 8 days after viral infection and CD8+ T cells were purified by magnetic bead selection (EasySep, STEMCELL Technologies). Fluorescence-activated cell sorting is used for the separation of tdTomato OT-I CD8+ cells from the CD8+ T cells of recipient WT mice.
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bioRxiv - Cancer Biology 2020Quote: ... The MNCs were pre-cleared of T cells and Monocytes by consecutive immunomagnetic negative selection (CD3 and CD14-EasySep StemCell Technologies) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... T cells were seeded at 2 × 106 cells/mL (typically 10×106 cells per well of a 6-well plate) and activated with ImmunoCult T Cell activator (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: NK-92 (ATCC®, CRL-2407™) were cultured in the ImmunoCult-XF T Cell Expansion Medium (STEMCELL Technologies Inc., USA) with the addition of Human Recombinant Interleukin 2 (IL-2 ...
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bioRxiv - Biochemistry 2023Quote: CD8+ T lymphocytes were enriched from lung cell suspensions prepared according to the manufacturer’s instructions with the CD8+ T cell isolation kit (Stem Cell Technologies). Purified CD8+ T cells were incubated in ELISpot plates in the presence of medium or a pool of S1 peptides (1 μg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were either harvested to assess levels of exhaustion marker expression by flow cytometry or DMSO-treated cells were purified using the mouse CD8 T cell isolation kit (StemCell Technologies). Enriched mouse CD8+ T cells were then treated with either DMSO alone or soquelitinib at concentrations ranging from 0 to 10 μM with additional two rounds of stimulation with the OVA peptide ...
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bioRxiv - Cancer Biology 2023Quote: CD8+ T cells were isolated from the spleens and lymph nodes of OTI mice using the EasySep Mouse CD8+ T Cell Isolation Kit (StemCell Technologies). For direct co-culture experiments ...