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Citations for Bio-Rad :
4851 - 4900 of 4995 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Samples were separated by SDS-PAGE electrophoresis at 100 volts (V) for 1 h on 4–20% Criterion™ TGX Stain-Free™ Protein Gels (1656001, Bio-Rad) and 1x SDS-page running buffer (192 mM glycine ...
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bioRxiv - Microbiology 2024Quote: ... Samples were boiled at 95°C for 5 min and resolved in 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). Proteins were transferred to a nitrocellulose membrane and probed overnight at 4°C with primary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Twenty micrograms of protein per sample were loaded into each lane of 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). The proteins were transferred to nitrocellulose membrane using semidry Trans- Blot® Turbo™ Transfer system (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... samples were placed on a heat block (+90°C) for 10 minutes and separated by SDS–polyacrylamide gel electrophoresis (4–20% Mini-PROTEAN TGX gels, Bio-Rad, 456-1096). Proteins were transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Physiology 2024Quote: ... Thirty µg of total protein from each sample was loaded into a 4–15% gradient Criterion Tris-Glycine extended (TGX) Stain Free gel (#5678085, Bio-Rad, Gladesville, Australia), separated via electrophoresis at 200 V ...
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bioRxiv - Neuroscience 2024Quote: ... 10-20 µg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... SDS-PAGE was performed by loading 20 µg of each sample into 4 – 15% Mini-PROTEAN® TGX™ Precast Protein Gels (#4561083, Bio-Rad) which were ran in tris/glycine/SDS running buffer (#1610772 ...
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bioRxiv - Cancer Biology 2024Quote: ... 30 ug of protein was loaded in each lane of a 4– 15% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad 4561083). After SDS-PAGE ...
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bioRxiv - Microbiology 2024Quote: ... A third of the total volume of each supernatant were boiled and run on 4 to 12% Tris/Glysine polyacrylamide gels (BioRad, Cat.#456-1093). Following electrophoresis ...
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bioRxiv - Physiology 2024Quote: ... Lysates were denatured at 95°C for 15 min and then electrophoresed on 4%–20% TGX stain-free polyacrylamide gel (Bio-Rad, Hercules, CA) in 1 × Tris/Glycine/SDS buffer (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... Both samples were reduced with 100 mM DTT and separated on a 4–20% TGX Stain-Free™ precast gels (Bio-Rad Laboratories). Proteins were then transferred to a nitrocellulose membrane and subjected to blotting using the NCR13 antibody.
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bioRxiv - Systems Biology 2024Quote: ... From each sample 20 µg of total protein was loaded and separated on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad), followed by electroblotting onto membranes of Trans-Blot Turbo Midi 0.2 µm PVDF Transfer Packs (Bio-Rad) ...
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bioRxiv - Bioengineering 2024Quote: ... were separated on a 4–10% gradient sodium dodecyl sulfate-polyacrylamide gel at 120 V for 90 min using a PowerPac Basic setup (Bio-Rad Laboratories, Inc.). Next ...
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bioRxiv - Physiology 2024Quote: ... Western blot preps (15 µL) were pipetted onto SDS gels (4-15% Criterion TGX Stain-free gels, Bio-Rad Laboratories; Hercules, CA, USA), and proteins were separated by electrophoresis at 180 V for 50 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein were loaded into each lane of a 4–20% gradient precast acrylamide SDS-PAGE gel (Bio-Rad, Cat #: 4561096). Gels were loaded with 2µL of Precision Plus ProteinTM Standard Dual Color ladder (BioRad ...
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bioRxiv - Immunology 2024Quote: Protein samples were prepared in Laemmli buffer and heated at 95°C for 5 min prior to resolution by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). Proteins were transferred onto nitrocellulose membranes (Biorad Trans blot turbo) ...
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bioRxiv - Genomics 2024Quote: ... A total of 10 µg of denatured protein was loaded onto Criterion TGX long shelf-life Precast Gels (4-15 %, Bio-Rad, Cat#5671083) using 1X Tris/Glycine/SDS buffer (Bio-rad ...
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bioRxiv - Genetics 2024Quote: ... 20-40µg of total proteins were separated with SDS-PAGE in 4-15% polyacrylamide gel and then transferred to polyvinylidenedifluoride (PVDF) membranes (Bio-Rad, Hercules, CA). The membranes were blocked with 5% non-fat milk and immunoblotted with primary antibodies against CFTR (769 from the UNC distribution program ...
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bioRxiv - Biochemistry 2024Quote: rhMOG was electrophoretically separated under reducing conditions using Mini-Protean 4-15% TGX Precast gels and a Mini-Protean Tetra Cell (Biorad, Hercules, California, USA). For total protein staining ...
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bioRxiv - Biochemistry 2024Quote: ... samples containing 20 µg of protein were electrically resolved to their pore size limit on precast 4-15% gradient PAG (Bio-Rad, UK, #4561085) in running buffer (25 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... Between 20 and 50 μg of protein was used for standard SDS– polyacrylamide gel electrophoresis procedures with Mini-Protean TGX gels (4 to 15%; Bio-Rad, 456-1084). An iBlot2 quick transfer system (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were heated to 95°C for 5 minutes and equal concentrations of samples (15μg for whole cell lysates and 5μg for nuclear fractions) were resolved on Criterion 4-20% gels (Bio-Rad, Cat# 5671093, and 5671094) and transferred to nitrocellulose (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... 20-40 µg protein were separated by SDS-PAGE on 4-15% Mini-PROTEAN® TGX Stain-Free™ Gels (Bio-Rad®) and transferred onto PVDF membranes using Trans-Blot Turbo RTA Mini LF PVDF Transfer Kit (Bio-Rad®) ...
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bioRxiv - Biochemistry 2023Quote: ... Volumes containing 10 µg of protein were resolved using SDS-PAGE (NuPAGE 3-12% gels, Life Technologies or 10% acrylamide Tris-Glycine, Bio-Rad for desmin fibrillar aggregates) and transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... Protein samples were heated for 3 min at 95°C and loaded on a 12-well Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad Laboratories, Inc, Hercules, CA, USA). SDS-PAGE followed by densiometric analysis using the software ImageJ32.
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bioRxiv - Microbiology 2022Quote: ... and 1’,3’-bis[1-palmitoyl-2-oleoyl-sn-glycero-3-phospho]-glycerol (16:0-18:1 Cardiolipin) were spotted using a Hamilton syringe onto a nitrocellulose membrane (Biorad trans-blot turbo RTA Midi 0.2 µm) to yield 10 ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was incubated with amylose resin for 1 h at 4°C and loaded onto a 20 ml chromatography column (Bio-Rad, Hercules, CA, USA). After washing 3 column volumes with wash buffer (50 mM Tris ...
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bioRxiv - Developmental Biology 2020Quote: ... 5μg of either whole brain lysate or tubulin-enriched protein fraction was loaded per lane and run on 4-20% Mini-Protean TGX Stain-Free precast gels (4568093; Bio-Rad Laboratories, Hercules, CA) at 150mV for 1hr ...
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bioRxiv - Cell Biology 2020Quote: Experiments were run using 26-well gels (Criterion TGX Precast Gels, 15 μl, 1.0 mm, 4-20%, Bio-Rad Laboratories, Inc. Cat No 5671095). 13 wells were loaded by one researcher and the other set of 13 wells were loaded by another researcher for each set of experiments ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was diluted 18-fold and 4 µL was used in a reaction for SYBR green detection with SsoAdvanced SYBR Green supermix (Bio-Rad, catalog no. 1725270) on a CFX384 machine (Bio-rad) ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was diluted 20-fold and 4 µL was used in a reaction for SYBR green detection with SsoAdvanced SYBR Green supermix (Bio-Rad, catalog no. 1725270) on a CFX384 machine (Bio-rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... Reducing SDS sample buffer was added and proteins resolved on 4%-15% Mini-PROTEAN TGX Stain-Free Precast Gels (Bio-Rad, California, United States) or 3%-8% NuPAGE Tris-Acetate Protein Gels (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of the resulting solution was then applied to either 4-12% Criterion™ XT Bis-Tris Protein Gels (BioRad, Hercules, CA, USA) or NuPAGE™ 4 – 12% Bis-Tris Mini Protein Gels (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... and one-dimensional electrophoresis was carried out for 4 hours at 75-100 volts in a Tris-glycine-SDS (Bio-Rad, catalog 161-0732) running buffer ...
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bioRxiv - Physiology 2020Quote: ... and equal amounts of total protein (15 or 20 μg) were loaded in different wells on Criterion™ 4-20% TGX Stain-Free™ Precast Gels (Bio-Rad, Australia). A stain-free system (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein lysates (25 μg) were separated in equal volume on 4 – 20% Mini-PROTEAN TGX™ Precast Protein Gels (Bio-Rad, cat. # 4561096) and transferred to an Immun-Blot polyvinylidene difluoride membrane (PVDF cat ...
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bioRxiv - Microbiology 2020Quote: ... Cell suspensions were clarified by centrifugation (20 000 x g, 10 minutes, 4°C) and total soluble protein quantified58 using a kit (Bio-Rad Protein Assay Kit) and BSA as a standard.
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bioRxiv - Cancer Biology 2020Quote: ... Samples were denatured for 5 min at 95°C and gel was run using a 4-20% Mini-PROTEAN ® TGX precast protein gels (Bio-Rad, No. 4561094). Next ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and electroporated in a 4-mm cuvette using an exponential pulse at 250 V and 950 mF in a Gene Pulser electroporation device (Bio-Rad Laboratories, Feldkirchen, Germany). We used a plasmid encoding a CMV-mCherry-Cas9 expression cassette and a human SAMHD1 gene specific gRNA driven by the U6 promoter ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins extracts were boiled at 95°C and then separated onto Mini-PROTEAN ® TGX™ precast protein SDS-PAGE (4-15% gradient) gels (BIO-RAD, #4561083). Proteins were transferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were clarified for 5 min at 10,000 × g at +4□C and protein concentration was determined using DC Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA). For immunoprecipitation ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of mESC lysate were separated on 4–12% or 10% SDS-PAGE gels (pre-cast NuPage® GE healthcare or Mini-Protean TGX Stain free precast gels, Biorad, Hercules, CA) and transferred to nitrocellulose (GE healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were solubilized in Laemmli sample buffer and heated at 95°C for 10 min to denature proteins and separated via SDS-PAGE for 40–60 min at 270 V using 4–20% stain-free gradient gels on Criterion electrophoresis cell (Bio-Rad Laboratories, Richmond, CA). Thereafter ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein lysates (25 µg) were separated in equal volume on 4 – 20% Mini-PROTEAN TGX™ Precast Protein Gels (Bio-Rad, cat. # 4561096) and transferred to an Immun-Blot polyvinylidene difluoride membrane (PVDF cat ...
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bioRxiv - Neuroscience 2021Quote: ... These samples were run on a 4–20% SDS-PAGE and electroblotted onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad Laboratories, Hercules, CA, USA) from the gel by a semidry blotting apparatus (Bio-Rad Laboratories) ...
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bioRxiv - Bioengineering 2021Quote: ... incubated for 5 min at 95°C and used as template in the qPCR reaction in a final 10−4 dilution with the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA). The qPCR was run in a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Physiology 2020Quote: ... Amplification reactions and disassociation curves of 4-fold dilution series of cDNA were carried out on a CFX384 machine (Bio-Rad, Bio-Rad, Mississauga, ON), for determination of primer efficiencies ...
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bioRxiv - Immunology 2020Quote: ... of protein were incubated in 1X Laemmli buffer with 0.1 M dithiothreitol at 65°C for 15 min and electrophoresed on 4-15% gradient TGX gels and transferred to PVDF membranes (Bio-Rad Laboratories, Mississauga, Canada). The membranes were blocked with Tris-buffered saline with 0.05% Tween 20 (TBST) ...
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bioRxiv - Biophysics 2020Quote: ... Pure ClyA-AS type-I (12-mer) nanopores were obtained using native PAGE on a 4-15% gradient gel (Bio-Rad, Veenendaal, The Netherlands) and subsequent excision of the correct oligomer band.
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bioRxiv - Genetics 2020Quote: ... diluted samples were boiled for 5min at 95 °C and subsequently separated on a 4-15% Criterion™ TGX Stain-Free™ Protein Gel (Bio-Rad Laboratories) and ran in a Criterion™ Cell geltank (Bio-Rad Laboratories ...