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Citations for Bio-Rad :
4501 - 4550 of 4995 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Nichols TPA lysates (5 x 107 spirochetes per lane) were resolved by SDS-PAGE using a 4-20% gradient Any kD Mini-Protean TGX gels (Bio-Rad) and transferred to 0.45 nm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: Equal amounts of bone marrow cell lysates underwent electrophoresis on a gradient SDS-PAGE gel (Criterion XT precast gel, 4-12%, BioRad, 3450123). Proteins were transferred onto nitrocellulose membranes and after blocking with Intercept (TBS ...
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bioRxiv - Cell Biology 2024Quote: SDS-PAGE of the peptide pull-down samples was performed using 4–20% Mini-PROTEAN TGX Stain-free Protein Gels (Bio-Rad). As a reference for molecular weights ...
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bioRxiv - Physiology 2024Quote: ... and 20 μg of proteins were separated by SDS-PAGE using 4-20% Criterion TGX Stain-Free Protein Gels (5678094, Bio-Rad). Total protein stains were activated by UV excitation using a ChemiDoc MP system (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... Total proteins (10 µg) were separated on a 4–20% SDS-PAGE gel (Bio-Rad, TGX Stain-Free™ precast gels) and transferred to a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2024Quote: ... Equal amounts of samples were run on SDS PAGE gels (BIO-RAD 4-12% gradient Bis-Tris or XT Precast gels with catalog numbers mples were run on SDS PAGE gels (BIO-RAD 4-12% gradient Bis-Tris or XT Precast gels with catalog numbers #3450123 or #3450124 ...
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bioRxiv - Genomics 2024Quote: ... the samples were treated with 5% 2-mercaptoethanol in Laemmli buffer and heated at 95°C for 5 minutes prior to loading into a 4-15% gradient SDS gel (Cat# 4568085, Bio-Rad) and run at 160v for 50-55 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... and gene-specific primer pairs (Table 4) on the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA). The mRNA levels were normalized with respect to Gapdh.
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bioRxiv - Biochemistry 2024Quote: ... The reaction was then passed through a P6 Micro Bio-Spin chromatography column (Bio-Rad, 1,000 x g, 4 min, rt), annealed in 1 x annealing buffer and prepared at 100 nM oligonucleotide concentration.
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bioRxiv - Biochemistry 2024Quote: ... 5 µL of the cleared lysates and 10 µL of washed beads after pull-down were applied on separate Mini-PROTEAN TGX 4-12% polyacrylamide gels (BIO-RAD), and run for 45 min at 200 V in Tris/Glycine/SDS buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... The same total protein amounts were loaded into each lane of 4–20% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Completed gels were transferred onto 0.2 um nitrocellulose membrane ...
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bioRxiv - Genetics 2024Quote: ... Samples were loaded at 15-20 µg per well onto a NuPAGE 4-12% Bis-Tris Acrylamide midi gel along with Precision Plus dual color ladder (BioRad, 1610374). The gel was run on an XCell4 Surelock Midi-Cell (ThermoFisher WR0100 ...
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bioRxiv - Cell Biology 2024Quote: ... The protein samples were separated on a 4–20% gradient Mini-Protean TGX Gel Stain-free (Bio-Rad, Hercules, CA, USA). The separated proteins were blotted on nitrocellulose membranes using the Trans-Blot Turbo RTA Transfer Kit (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: 40-50 µg of cleared cell lysates were separated on 4-20% gradient gels (Mini-Protean or Criterion TGX, Bio-Rad) by SDS-PAGE and transferred to nitrocellulose for immunoblotting ...
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bioRxiv - Cancer Biology 2024Quote: ... and equal amounts of protein were prepared to SDS-Page using a 4-20% gradient polyacrylamide gel (Bio-Rad, Hercules, CA) and transferred to a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2024Quote: ... About 100 μg of protein with 2x serial dilutions were loaded onto 4–20% TGX™ precast mini gels (BioRad, England) either with or without reduction with 50 mM dithiothreitol (DTT) ...
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bioRxiv - Biochemistry 2024Quote: ... 20% (v/v) glycerol and 0.01% (w/v) bromophenol blue) and loaded onto Mini-PROTEAN TGX™ 4–20% precast gels (Bio-Rad). As a reference ...
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bioRxiv - Cell Biology 2024Quote: Samples then were subjected to SDS-PAGE using a 4% to 15% Criterion TGX Stain-Free Precast gel (Bio-Rad, 5678084), then transferred onto a PVDF membrane (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: Samples were combined with Laemmli Sample buffer + 10% BME and separated on pre-cast 4-20% SDS- PAGE gels (Bio-Rad). Gels were transferred onto PVDF membranes (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were analyzed on either a 12% Bis Tris PAGE gel (for histone analytes) or a 4-12% Bis Tris PAGE gel (for all other analytes) using 1x MES running buffer (Bio-Rad) at 180 V ...
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bioRxiv - Cancer Biology 2024Quote: Western blotting was performed using 10 µg protein lysate (25 µg for detecting DARPins) mixed with 4 x Laemmli sample buffer (Bio-Rad) and diluted to 1 x with dH2O and addition of β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... The proteins were separated by SDS-polyacrylamide gel electrophoresis (4–15% Mini-PROTEAN® TGX™ Precast Protein Gels, BIO-RAD), transferred to nitrocellulose ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein lysates were centrifuged (20,000 g, 20 min, 4°C) and protein concentrations were obtained using Quick Start Bradford Dye Reagent (Bio-Rad, 5000205). Normalized protein samples were resolved on 4-20% tris-glycine gels and transferred to Amersham Hybond P 0.45 PVDF blotting membranes (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were resolved by SDS-PAGE on 4-15% gradient Mini-PROTEAN® TGX precast protein gel (Bio-Rad, cat. 4561086) in 1x Tris/glycine/SDS buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were boiled at +90 °C for 5 min prior to protein separation using precast SDS- PAGE gradient gels (4–20% Mini-PROTEAN TGX, Bio-Rad) and transferred onto nitrocellulose membranes with the semi-dry Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Resultant RNA-Protein (RNP) complexes were resolved by native polyacrylamide gel electrophoresis (4-15% Tris-Glycine gradient gels, Bio-Rad #5671084) at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... The plate was sealed with a pierceable seal (4titude; Wotton, UK) for 3 s at 180°C by using a plate sealer (BioRad PX-1; CA, USA) and maintained at 4°C during the LC-MS measurement ...
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bioRxiv - Microbiology 2020Quote: ... The plate was washed 3 times prior to adding 50 μl of 1:1000 diluted Goat anti-Mouse IgG H+L-HRP antibody (Bio-Rad, Cat# 172-1011) to the plate and incubated for 1 h at RT ...
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bioRxiv - Systems Biology 2021Quote: ... incubated 5 min at 65 °C and separated by 12% SDS-PAGE (65) in the Mini Protean 3 Apparatus (BioRad Laboratories, Hercules, CA, USA). The final concentration of proteins was 10 μg per lane ...
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Biallelic loss-of-function OBSCN variants predispose individuals to severe, recurrent rhabdomyolysisbioRxiv - Genetics 2021Quote: Frozen muscle biopsies were homogenised in modified Laemmli sample buffer as described previously.37 Samples were run in Bio-Rad Criterion 3–8 % tris-acetate gradient gels (Bio-Rad Laboratories, CA, USA) at room temperature ...
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bioRxiv - Genetics 2022Quote: ... the membranes were washed 3 times for 10 min each with TBST buffer at 24 °C and visualized using enhanced chemiluminescence reagents (Bio-Rad Laboratories, CA, USA). The images were captured using Image Quant LAS 4000 (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2021Quote: ... and O’nyong yong virus (ONNV) were subjected to a thermal gradient treatment from 30 to 60 °C for 3 h with a thermocycler (Bio-Rad T100 Thermal Cycler), after which samples were immediately titrated on Vero cells ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg RNA was transferred into the cDNA Ecodry Premix Kit prior to the quantitative PCR program being run on ABI QuantStudio 3 with iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, 1725121). Each reaction proceeded at 50 °C for 2 min ...
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bioRxiv - Cancer Biology 2024Quote: ... and the miR200c primers (upstream primer, 5’- TAATACTGCCGGGTAATGATGGA-3’) (Eurofins Genomics) on the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). U6 primers (TAKARA ...
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bioRxiv - Bioengineering 2023Quote: ... The plate was sealed with a pierceable seal (4titude; Wotton, UK) for 3 seconds at 180°C by using a plate sealer (BioRad PX-1; CA, USA) and maintained at 10°C during the LC-MS measurements ...
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bioRxiv - Microbiology 2023Quote: ... One microliter of each sample was mounted on a layer of 1.2% (w/v) agarose in 1:3 (vol/vol) TSB/PBS placed on a glass plate (Bio-Rad Mini-PROTEAN Short Plate) with a coverslip placed on top of each sample ...
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bioRxiv - Bioengineering 2023Quote: ... The plate was sealed with a pierceable seal (4titude; Wotton, UK) for 3 s at 180 °C using a plate sealer (PX-1; Bio-Rad, Hercules, CA, USA) and maintained at 10 °C during the LC-MS measurements ...
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bioRxiv - Molecular Biology 2024Quote: ... The same AAV2 ITR primers were used for ddPCR along with a FAM labeled probe 5 ’-CACTCCCTCTCTGCGCGCTCG-3 with the QX200 Bio-rad Laboratories’ droplet digital system (Bio-rad Laboratories, California, USA) and ddPCR supermix for probe (Bio-rad Laboratories ...
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bioRxiv - Plant Biology 2020Quote: ... and 0.4 μM final concentrations of the gene-specific PCR primers on a CFX Connect Real-Time System thermal cycler (Biorad, Hercules, California). The qPCR conditions were 95°C for 3’ ...
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of total protein per lysate were loaded onto Criterion XT 4-12% Bis-Tris polyacrylamide gels (Bio-Rad, Hercules, CA), transferred onto PVDF membranes (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... The monolayer was fixed with 4% paraformaldehyde and permeabilized with Triton-X100 and stained with mouse anti-NP IgG1 (clone AA5H, Bio-Rad Antibodies) followed by goat anti-mouse HRP (DAKO ...
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bioRxiv - Genetics 2021Quote: ... Forty micrograms of total protein from hippocampal nuclear lysates was boiled for 5-minutes to dissociate complexes before separation in a 4-20% gradient gel (Bio-Rad 4561096) for 1-hour at 150V in 1x Tris/Glycine/SDS (Bio-Rad 1610732) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were boiled at 95°C for 5 minutes with Laemmli buffer and 20 μg of each was loaded into a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BioRad, Hercules, CA). Gels were run at 100 V for 30-60 minutes and then transferred to PVDF membranes for 2 hours at 250 mV ...
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bioRxiv - Developmental Biology 2021Quote: ... Additional pre-absorption controls were performed in which the anti-IL-6 antibody was incubated overnight at 4 °C with a 10 fold molar excess of recombinant chicken IL-6 (1.67 μM; Bio-Rad Laboratories, Inc.) before immunolabeling fixed sections of chick ocular tissues ...
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bioRxiv - Neuroscience 2021Quote: ... samples heat denatured and 20 µg of total protein was loaded per lane on 4-20% Tris-HCl polyacrylamide gels (BioRad, California, USA). After transfer to PVDF ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotinylated proteins were then eluted by boiling the magnetic beads in 30 μL 4 χ Laemmli sample buffer (Bio-Rad, Cat.# 1610747) supplemented with 20 mM DTT and 2 mM biotin.
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bioRxiv - Cell Biology 2022Quote: Protein homogenates (5 or 15 μg/lane) were size fractionated on 4–15% or 15% Criterion TGX gels (Biorad Laboratories, Oslo, Norway) and transferred to 0.45 μM PVDF-membranes (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... for the experiments done on 12-well plate) for each sample on SDS-PAGE using 4–20% Mini-PROTEAN® TGX gels (Bio-Rad), and immunoblotted as described (36) ...
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bioRxiv - Physiology 2020Quote: ... tissue biopsies were transferred into the assay medium for a 4h incubation (pre-incubation medium supplemented with 4 mg/ml glucose and 4.3% Alamar Blue solution (Bio-rad, Hercules, CA)) ...
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bioRxiv - Biochemistry 2020Quote: ... The samples collected before and after UV-crosslinking were subsequently analysed by SDS-PAGE in 4-20% gradient gels (Bio-Rad TGX). Gels were subsequently transferred onto a PVDF membrane and FLAG epitopes were detected by Sigma M2 antibodies followed by ECL imaging.