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Citations for Bio-Rad :
4951 - 4995 of 4995 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... CD45-positive and CD3-positive cells were centrifuged at 400 x g for 7 minutes at 4 ºC and counted with a TC20™ Automated Cell Counter (Bio-Rad Laboratories, S.A), before proceeding to single cell RNA sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... a mixture of 250 μL of this cell suspension and 1000 ng of a vector plasmid digested with XbaI and KpnI enzymes was chilled on ice for 5–10 minutes within a 4-mm width cuvette compatible with the Gene Pulser®/MicroPulser™ system (BioRad, Hercules, CA). The cell-DNA mixture was then electroporated using the GenePulser XCell™ device (BioRad ...
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bioRxiv - Biophysics 2024Quote: ... before boiling the sampling in a reducing sample buffer and separation on a 4-15 % mini-PROTEAN Precast SDS-PAGE (Bio-Rad, Cat No 4561085). Transfer to PVDF membrane was performed using a Trans-Blot pack (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... and 15ug of denatured protein lysate was separated on a 4-15% Mini-Protean TGX Precast Protein Gel (Cat# 4561086, Bio-Rad Laboratories, Inc. USA). After transfer to 0.2 um PVDF membrane using TransBlot Turbo Transfer system (Cat# 1704272 ...
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bioRxiv - Cell Biology 2024Quote: ... and boiled for 5 min before loading into a 12% or 4-20% acrylamide pre-cast SDS- PAGE gels (Bio-Rad, 4561045 or 4568094) for electrophoresis ...
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bioRxiv - Cell Biology 2024Quote: ... The lysates were centrifuged at 16,100 rcf for 15 minutes at 4 °C and protein concentration was quantified using the DC™ Protein Assay kit (Bio-Rad, Hercules, CA).
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bioRxiv - Biochemistry 2024Quote: Cell lysates containing 1X SDS buffer were homogenized with 27-G syringe and whole lysates were run on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4568096) or 6/10/12% home-made polyacrylamide gels before being transferred to PVDF membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: Western blotting was performed using 4–20% Mini-PROTEAN® TGX™ precast protein gels and the Trans-Blot® Turbo™ transfer system (Bio-Rad). The following antibodies ...
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bioRxiv - Cell Biology 2020Quote: Equal amounts of protein were loaded onto 12% (v/v) resolving SDS-PAGE gels with 4% (v/v) polyacrylamide stacking gels using Precision Plus Protein™ dual colour standards (Bio-Rad, Hercules, CA, USA). The gels were run in a Mini-Protean® Tetra Cell system (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Purified proteins were eluted in SDS-PAGE sample loading buffer and separated in 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad# 4561094, 4561096) either for western blot (WB ...
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bioRxiv - Biochemistry 2021Quote: Cell lysates were prepared using RIPA buffer (see lysis and click chemistry methods below) and run on SDS Tris-Glycine gels (Precast 4 - 20% Bio-Rad, or hand-casted 20%). Gels were transferred to low-background PVDF membranes using the BioRad TransBlot Turbo system and blocked with 5% milk powder in TBS + 0.1% Tween 20 (TBST ...
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bioRxiv - Microbiology 2020Quote: Twenty-fold concentrated viral stocks or purified rgp120 were separated by SDS-PAGE under reducing conditions using a 4-15% Mini-PROTEAN TGX precast gel (Bio-Rad Laboratory Inc. Hercules, CA), and then transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2022Quote: ... and were heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (Bio-Rad, 4–15% Tris/Glycine) and then transferred onto 0.2 µm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were clarified at 13,200 rpm for 15 min at 4°C prior to quantification by Lowry assay (Bio-Rad cat # 5000113 and cat # 5000114). Whole cell lysates were loaded into 4-20% Criterion TGX Precast 18 well gels (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS and incubated overnight at 4°C in primary antibodies: anti-human actin-β primary monoclonal antibody produced in mouse (1:100; Bio-Rad Laboratories Inc. MCA57766A) and non-muscle myosin II produced in rabbit (1:100 ...
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bioRxiv - Physiology 2020Quote: ... Amplification reactions and disassociation curves of 4-fold dilution series of cDNA were carried out on a CFX384 machine (Bio-Rad, Bio-Rad, Mississauga, ON), for determination of primer efficiencies ...
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bioRxiv - Physiology 2020Quote: ... and an equal amount of total protein (30 μg) per sample was subjected to standard SDS-PAGE using Mini-PROTEAN® TGX™ 4-20% 12-wells gels (Bio-Rad, Hercules, CA). Gels were electroblotted onto PVDF membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... resolved by SDS-PAGE (4-15% acrylamide, 150V) and transferred to PVDF membranes using the Trans-Blot® Turbo™ Transfer System (Bio-Rad Laboratories, CA). Membranes were incubated for 1 hr in 5% milk (in TBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... pH 9.5). Quantification of Western blot images (SFig. 1-4) was carried out using ChemiDoc™ XRS+ Image Lab™ Software (Bio-Rad Laboratories AB, Sweden); statistical analysis was performed with GraphPad Prism software (version 5.0 ...
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bioRxiv - Developmental Biology 2020Quote: ... was performed using the primer pairs listed in Table 4 and iTaq Universal SYBR Green Master Mix on a CFX384™ Real-Time PCR detection system (Bio-Rad Laboratories, Hercules, CA). Fold changes in gene expression were calculated by the ΔΔCt method using the weighted average of Ct values for the housekeeping genes Pol2ra ...
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bioRxiv - Neuroscience 2021Quote: ... or 40 μg (splenocytes of WT and Pik3cd−/− mice) of lysate were loaded onto 4-15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories B.V.). Transfer was performed onto Immobilon®-P PVDF Membranes (Merck KGaA) ...
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bioRxiv - Neuroscience 2022Quote: ... and protein standards were mixed with Laemmli buffer and loaded on 4-15% Criterion TGX Stain-Free protein gels in 1×□TGS running buffer (all from Bio-Rad, Marnes-la-Coquette, France) and transferred to a 0.20μm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: For each lane, ∼20 μg protein were run on a 4-12Bis-Tris Criterion XT Precast Gel (#3450123, Bio-Rad (Bio-Rad, Hercules, CA, USA) next to the Precision Plus Protein Dual Color Standard (catalog#161-0374 ...
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bioRxiv - Neuroscience 2023Quote: ... from cells or brain tissue were separated on SDS polyacrylamide gels (4–15% Mini-PROTEAN® TGX Stain-Free™ gels, Bio-Rad, Hercules, USA) and transferred onto a membrane (nitrocellulose or PVDF ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from cell extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on 4-20% gradient gels (Bio-Rad #4561095; Hercules, CA, USA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 μg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 µg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... 15 micrograms of total protein were separated using stain-free 4–15% gradient Tris-glycine SDS-polyacrylamide gels (Mini PROTEAN® TGX Stain-Free™ Gels, Bio-Rad Laboratories, CA) and transferred onto PVDF membranes (Amersham Hybond ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 20 μg of proteins were analyzed in a polyacrylamide gel (4–20% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, Marnes-la-Coquette, France) and separated by electrophoresis ...
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bioRxiv - Biophysics 2024Quote: ... the solution was heated and slowly cooled from 80°C to 4°C (ramp rate of -1°C per 5 s) in a standard thermocycler (Bio-Rad CFX96 Real-Time System) prior to each experiment.
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bioRxiv - Neuroscience 2024Quote: ... 50 µL of media was reduced and denatured prior to gel electrophoresis on a 4-15% tris-glycine gel (#4561084, Bio-Rad Laboratories, Hercules, CA, USA) for 2 hours @ 175 mV ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then incubated overnight at 4°C with antibody cocktail consisting of rat anti-F4/80 (1:100) (catalog #: MCA497; Bio-Rad Laboratories; Hercules, CA, USA), rabbit anti-vimentin (1:100) ...
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bioRxiv - Biochemistry 2024Quote: ... 20 to 30 µg of total protein was separated by SDS-PAGE on a pre-cast 4–15% Criterion™ Protein Gel (Bio-Rad Laboratories, Cat#5671084) followed by an overnight transfer and western blot analysis.
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bioRxiv - Neuroscience 2024Quote: ... were separated under reducing conditions by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) (4-20% Criterion TGX Stain-free gels, Bio-Rad Laboratories, Hercules, CA, USA) and transferred to Polyvinylidene fluoride (PVDF ...
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bioRxiv - Genetics 2024Quote: ... 30μg total protein from tissue samples with 4X Laemmli buffer was heat denatured at 99°C for 5 minutes and ran on resolving SDS PAGE gel (Biorad 4-20% precast gel #4561096). Sperm protein samples were prepared using a low volume of lysis buffer (∼100) ...
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bioRxiv - Biochemistry 2024Quote: ... Equal amounts of protein were subjected to 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (cat. no. 1620112; Bio-Rad Laboratories, Hercules, CA, USA). The membranes were blocked using Intercept® (TBS ...
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bioRxiv - Immunology 2021Quote: Proteins (20 µg per well) were separated in 4–15% Criterion™ TGX™ Precast Midi Protein Gels with 26 wells (Bio-Rad, Hercules, CA, USA) at a constant voltage of 120 V ...
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bioRxiv - Microbiology 2020Quote: ... 8.4 μL of PCR Probe water and 10 μL of the 2x commercial reaction mixture (SYBR® Green Master Mix, Bio-Rad. A LightCycler96® (Roche) device was used and PCR conditions applied were ...
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bioRxiv - Neuroscience 2020Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min before loading into a 4 – 20% Criterion TGX precast Midi protein gel (Bio-Rad, Hercules, CA, USA; Cat# 5671094) for SDS-PAGE followed by wet transfer to a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2021Quote: ... Beads were incubated 5 min at 95 °C in this solution and 20 µL of supernatant was analyzed on gradient gels (BioRad 4-12% TGX pre-cast gels). The modified proteins were detected with an infrared imager (Odyssey ...
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bioRxiv - Biochemistry 2021Quote: The proteins extracted from Ni-NTA resin were subjected to SDS-PAGE on 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Hercules, CA, USA) using the standard procedure ...
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bioRxiv - Microbiology 2022Quote: ... The lysates were adjusted to 5 µg of total soluble proteins in native sample buffer and then loaded onto a 4-20% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad, Cat. No. 4561094). Protein electrophoreses were performed in TG buffer (60 mM Tris ...
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bioRxiv - Cancer Biology 2023Quote: ... Ten micrograms of total protein extract underwent electrophoresis in stain-free 4–15% gradient Tris-glycine SDS-polyacrylamide gels (Mini PROTEAN® TGX Stain-Free™ Gels, Bio-Rad Laboratories, Hercules, CA) and transferred onto PVDF membranes (Hybond-C Extra ...
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bioRxiv - Immunology 2021Quote: ... with 0.5 μl of b-mercaptoethanol per well and heated at 70°C for 10 minutes before separation on a polyacrylamide gel (Bio-Rad Mini-PROTEAN TGX Gel 4-15%) and transferred to a PVDF membrane ...
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bioRxiv - Immunology 2024Quote: ... were then separated by size via SDS-polyacrylamide gel electrophoresis (SDS-PAGE) using a 4-20% Midi-PROTEAN® TGX Stain-Free™ gel (Bio-Rad Laboratories; Cat. No. 17000436). Trans-Blot® Turbo Transfer System™ (Bio-Rad Laboratories ...