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Citations for Bio-Rad :
4801 - 4850 of 4995 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and mixed 1:1 with 2X Laemmli buffer followed by SDS-PAGE using 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels and staining with Coomassie Brilliant Blue R-250 (Bio-Rad) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
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bioRxiv - Microbiology 2022Quote: ... Marcy l’Etoile, France), sheep blood agar with colistin and nalidixic acid (CNA, bioMérieux) and chromogenic agar (Uriselect 4, Bio-Rad, Marnes-la-Coquette, France). Bacterial growth was determined after 18 h of incubation at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min prior to loading into into a 4-15% Criterion TGX precast Midi protein gel (Bio-Rad, Cat#5671094) for SDS-PAGE followed by wet transfer to a nitrocellulose membrane ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lysates were loaded onto 4-12% Criterion XT precast gels and resolved by SDS-PAGE under reducing conditions (Bio-Rad Laboratories, Hercules, CA). The proteins were then electro-blotted onto a 0.45 µm nitrocellulose membrane ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins immunoprecipitated with anti-FLAG antibody were separated by SDS-PAGE (4-15% Mini-PROTEAN®TGX™ Precast Protein Gels (Bio-RAD)) ...
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bioRxiv - Neuroscience 2022Quote: Protein sample was loaded into each well of a 4-20% Mini-PROTEAN TGX Precast protein 15-well gels (Bio-Rad, cat#4561096). Protein was transferred using transfer buffer (20% MeOH in TBS ...
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bioRxiv - Pathology 2024Quote: ... 15 µg of proteins were resolved on Criterion TGX Stain-Free 4-15%,18 and 26-well gel (Bio-Rad Laboratories, #5678084, #5678085) in a Criterion large format electrophoresis cell (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... 4 µl of the diluted cDNA was added to a Master mix containing 4 µl SsoAdvanced Universal SYBR® Green Supermix (Bio-rad #1725270) and 0.5 µl of a 10 µM stock of each Forward and Reverse primer (Table 5 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... A total of 20 μg of protein was separated using a 4%–15% Criterion™ XT tris-glycine protein gel (BioRad, United States) and then transferred to nitrocellulose membrane (BioRad ...
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bioRxiv - Physiology 2024Quote: ... Then we separated proteins into 4-15% gradient SDS-PAGE gels and transferred them to PVDF immuno-blot membranes (Bio-Rad, Hercules, CA). The primary antibodies S6 (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: Nuclear lysates of inputs and affinity-purified elution were loaded onto 4-15 % Criterion TGX Stain-Free precast polyacrylamide gels (BIO-RAD, Cat# 5678085) and transferred to nitrocellulose membranes which were subsequently blocked by 5% BSA for 1Chour at RT ...
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bioRxiv - Immunology 2024Quote: ... of cells lysates was subjected to electrophoresis on a sodium dodecyl sulfate (SDS) 7.5% or 4-20% polyacrylamide gel (4561024 or 4561095, respectively, Bio-Rad, California, United States). Proteins were semi-dry transferred to PVDF membranes (1704159SP5 ...
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bioRxiv - Molecular Biology 2024Quote: ... Twenty micrograms of whole cell extracts were resolved on 4−12% SDS-polyacrylamide gels and transferred onto Ready gel blotting sandwiches immune-blot PVDF membranes (Biorad, Hercules, CA, USA) using a XCell II™ Blot Module (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 μg protein were subjected to electrophoretic run using a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories), transferred to PVDF membranes using the Trans-Blot® Turbo™ Midi PVDF Transfer Packs (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2023Quote: Proteins within tailocins were separated using a 4-20% sodium dodecyl sulfate-polyacrylamide gradient gel (Mini-PROTEAN TGX Stain-Free Precast, Bio-Rad Hercules, USA) electrophoresis (SDS-PAGE ...
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bioRxiv - Genetics 2024Quote: ... were loaded in parallel with with pre-stained molecular weight markers and resolved by SDS-PAGE in premade 4–15% gradient Criterion gels (BioRad, Hercules, CA, USA). Resolved tissue homogenates were transferred onto polyvinylidene difluoride (PVDF) ...
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bioRxiv - Bioengineering 2023Quote: ... and boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-20 µl of the cell lysates were separated on 4-15% Mini-PROTEAN® TGX™ Stain-FreeTM Protein Gels from Bio-Rad and transferred to methanol-soaked polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... and boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The equivalent of 1.107 parasites were loaded per lane in a 4–20% Mini-PROTEAN® TGX Stain-Free™ gel (Bio-rad, 4568096) and proteins were separated and transferred onto a nitrocellulose membrane using the transblot semi-wet transfer system (Biorad) ...
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bioRxiv - Neuroscience 2022Quote: ... 20 µL of protein lysate was then loaded into 4–20% Criterion™ TGX Stain-Free™ precast gels (Bio-Rad, Cat. #5678094). Proteins were separated at 200V for 45 min and then transferred to a low-fluorescence PVDF membrane at 25V for 30 min using the Trans-Blot Turbo Transfer System and Kit (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... and separated in polyacrylamide gels at 100-140 V on ice (4-15%, Mini-PROTEAN TGX, Bio-Rad; or as described previously [12]). To detect protein-bound acrolein in spinal cords ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates cleared by centrifugation (20 µL) were resolved by electrophoresis using 4-20% mini-Protean TGX gels in Tris/Glycine/SDS buffer (Bio-Rad, Hercules, USA). Gels were then transferred onto 0.2 µm nitrocellulose membranes using Trans-Blot Turbo Transfer System gel (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... then centrifuged at 13,000 rpm for 30 min at 4 °C and the resulting supernatants were subjected to Bradford protein assay (Bio-Rad, Hercules, California, USA) for measuring total protein concentration ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was collected and incubated at 95°C for 3 min in SDS sample buffer before separation by SDS–PAGE on 4–15% gradient Mini-PROTEAN TGX precast polyacrylamide gels and transferred to nitrocellulose membranes (Bio-Rad, Hercules CA). Membranes were next blocked with 5% milk in TBS with 0.1% Tween 20 and incubated with primary antibodies and then HRP-coupled secondary antibodies in 5% milk or bovine serum albumin in TBS with 0.1% Tween 20 ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal quantity of the protein was resolved in a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels and transferred to an Immun-Blot PVDF Membrane (Bio-Rad) for antibody incubation ...
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bioRxiv - Neuroscience 2023Quote: ... heated for 5 min at 95°C and loaded onto 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Germany). Proteins were transferred on nitrocellulose membranes and membranes were blocked with 5% BSA in PBS-Tween ...
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bioRxiv - Immunology 2023Quote: ... Same protein amounts were loaded onto 4-12% NuPAGE Gels and protein was transferred to PVDF membranes by using Trans-Blot® Turbo™ Transfer System (Biorad#17001917). Membrane was blocked with 5% milk-TBS ...
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bioRxiv - Molecular Biology 2023Quote: 20-25μg of protein lysate with 4x Laemmli buffer (cat# 1610747; Bio-Rad Laboratories was separated on 4-15% Mini-PROTEAN TGX Precast Protein Gels (cat# 4561086; Bio-Rad Laboratories) and transferred to Immun-Blot PVDF Membranes for Protein Blotting (cat# 1620177 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 16,000Xg for 30 min at 4°C then protein concentrations were determined by Quick Start™ Bradford 1x Dye Reagent (Bio-Rad #5000205). For each sample ...
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bioRxiv - Genetics 2023Quote: ... Co-IP reactions were rotated overnight at 4°C with 100 µl of SureBeads Protein G Magnetic Beads (BioRad; Catalog# 1614013; Hercules, CA). The bead lysates were washed five times in the co-IP buffer using a DynaMag Magnet (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... Bromophenol blue buffer was loaded on 1.0 mm thick Criterion XT Tris acetate (poly-acrylamide) gels with a linear resolving gel gradient of 4-12% (Biorad; Lunteren, The Netherlands). Gels were run at 75 V (0.07 A ...
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bioRxiv - Developmental Biology 2023Quote: Proteins from worm lysates or coIP experiments were separated on 10% or 4-20% gradient Mini-PROTEAN TGX Precast Gels (Bio-Rad Laboratories, Inc.) at 300V ...
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bioRxiv - Biochemistry 2023Quote: Final carboxysome samples and as well as the lysate were analyzed for presence of CsoSCA or sfGFP-fusion protein by SDS-page (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad)) ...
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bioRxiv - Bioengineering 2023Quote: ... at 100V with a constant current for 60 mins at 4°C in a Bio-Rad Mini-Transfer Cell (Bio-Rad, Cat# 1703930) according to the manufacturer’s specifications ...
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bioRxiv - Immunology 2023Quote: ... Primary antibody staining was performed overnight at 4&C using rabbit anti-mouse Iba-1 (1:500; Wako Chemical Cat #019-19741) and CD68 (1:200; Bio-Rad Cat #MCA1957) with secondary staining performed for 45 minutes RT using Alexa Flour 594 donkey anti-rabbit (1:400 ...
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bioRxiv - Genomics 2023Quote: ... 4 μl of 1:10 dilution of cDNA was mixed with SsoAdvanced Universal SYBR Green Supermix (cat no 1725271, Bio-Rad, Hercules, CA) and 500 nM each primer up to a total volume of 10 μl and amplified in a CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C in a humidified chamber with the following primary antibodies: Guinea-pig anti-Insulin antibody (Biorad 5330-0104G, 1:250). Following overnight incubation ...
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bioRxiv - Synthetic Biology 2023Quote: ... The centrifuged samples (10 μL sample volume) were loaded into a 4-15% gradient Mini-Protean TGX precast protein gel (Biorad, CA, Cat # 4561086) and run at 140 V ...
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bioRxiv - Plant Biology 2023Quote: ... Fifty micrograms of protein for each treatment was loaded onto a (4-20)% acrylamide SDS-PAGE denaturing protein gel (Bio-Rad, Hercules, USA) and separated at 4°C at 150 constant volts ...
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bioRxiv - Biophysics 2023Quote: ... Cleavage of LD650-labelled GPΔTM was verified by in-gel fluorescence imaging of uncleaved and cleaved protein on a 4-20% polyacrylamide using a ChemiDocTM MP imaging system (Bio-Rad, Hercules, CA) followed by Coomassie staining ...
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bioRxiv - Immunology 2023Quote: ... and equal protein amounts were separated using ready-to-use 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Biorad, 4568096) and transferred onto 0.2 μm pore nitrocellulose membranes (PALL Life Sciences 66485).
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bioRxiv - Genomics 2023Quote: ... 20-25ug of protein lysate was loaded per well and separated on a 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gel (Bio-Rad). Proteins were transferred onto an activated PVDF membrane using the Trans-Blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 μg protein were separated by SDS-PAGE on 4-15% Mini- PROTEAN® TGX Stain-Free™ Gels (Bio-Rad®). and transferred onto PVDF membranes using Trans-Blot Turbo RTA Mini LF PVDF Transfer Kit (Bio-Rad®) ...
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bioRxiv - Neuroscience 2023Quote: ... Equal volumes of the purified fractions were analyzed on a polyacrylamide gel (4–20% TGX Precast Gels, Bio-Rad, Marnes-la-Coquette, France). Total actin was then determined by immunoblotting actin for each experimental condition ...
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bioRxiv - Neuroscience 2023Quote: ... were pipetted into wells of a 18-well Criterion™ TGX Stain- Free™ pre-cast gel (4-15%, BioRad Cat. No. 5678084) and separated by SDS-PAGE ...
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bioRxiv - Neuroscience 2024Quote: ... boiled for 3 minutes at 95°C and directly loaded into a 10 well Mini-Protean TGX Precast Gel with a 4-15% polyacrylamide gel gradient with 50 µl volume per well (Biorad, Cat#456-1084). The gel was run in running buffer (composition below ...
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bioRxiv - Neuroscience 2024Quote: ... was then added and the samples were boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... and 8ug of total protein from each lysate was separated on a 4-15% SDS-polyacrylamide gel (Mini-PROTEAN® TGX™ Precast Protein Gels (BIO-RAD). After the proteins were transferred to nitrocellulose membrane (BIO-RAD) ...