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Citations for Bio-Rad :
4701 - 4750 of 4995 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Seven and a half μl of each super-natant were loaded on a 4-20% TGX Stain-Free precast gel (Bio-Rad, 4568096). Separated proteins were transferred onto PVDF membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... an equal number of cells were loaded and run on an SDS-PAGE gel (Bio-Rad #4561086: 4-15 Mini-PROTEAN TGX) along with Precision Plus Protein Dual Color Standards (BIO-RAD ...
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bioRxiv - Neuroscience 2024Quote: ... and 20 µg protein was used per well of a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad). Protein denaturation was carried out using 8 M Urea in 1x Laemmli Sample Buffer (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... Droplets were allowed to rest at 4°C for at least 30 minutes after cycling and were then read using the QX200™ Droplet Reader (Bio-Rad). Data were analyzed using QuantaSoft™ software ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were boiled for 10 min prior to separation by SDS-polyacrylamide gel electrophoresis (PAGE) using 4–20% precast polyacrylamide gels (Bio-Rad, #4561094) with Laemmli running buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The denatured protein samples were allowed to cool down and equal amounts of samples were analyzed by 4–15% or 7.5% TGX gels (Bio-Rad #4561084DC, #4561023DC) for sequential western blots on the same membranes ...
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bioRxiv - Physiology 2024Quote: ... cooled on ice and equal volume was loaded on 4-12% Midi-PROTEAN TGX stain-free protein gels (cat# 4568093, Bio-Rad Laboratories); the gel was then transferred to PVDF membranes using the Trans-Blot Turbo Transfer System(BIO-RAD ...
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bioRxiv - Neuroscience 2024Quote: ... Vector purity was assessed by 4–12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and fluorescent staining (Oriole, Bio-Rad, Hercules, CA, USA). A transfer plasmid (pAAV-CMV-eNpHR3.0-EYFP-WPRE ...
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bioRxiv - Immunology 2024Quote: Serum SARS2-specific binding antibody assays were performed using the Bio-Plex Pro Human SARS2 IgG 4-Plex Panel serology assay (Bio-Rad 12014634) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Proteins from collected SMC-MVs and Ost-MVs were separated in a 4–20% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel (Bio-Rad, USA), and subjected to consequent analysis by liquid chromatography with tandem mass spectrometry (LC-MS/MS) ...
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bioRxiv - Biochemistry 2024Quote: ... and boiled at 95° C for 5 minutes before separation on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). In-gel fluorescence was imaged first ...
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bioRxiv - Molecular Biology 2024Quote: ... and separated by native gel electrophoresis in TG buffer using 4–20% Mini-PROTEAN® TGX Stain-Free™ gels (Bio-Rad), subjected to 30 min pre-run ...
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bioRxiv - Molecular Biology 2024Quote: ... were denatured at 95°C and then separated via SDS-PAGE on the 4-20% Mini-Protean TGX Gels (Bio-Rad #4561096). For molecular weight estimation ...
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bioRxiv - Molecular Biology 2024Quote: Fifty μg of protein lysate per sample was separated by reducing electrophoresis on 4–20% polyacrylamide gels (Bio-Rad Laboratories, # 456–8096). Protein was transferred from gels to polyvinylidene fluoride membranes (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... the membrane was incubated overnight at 4°C in blocking buffer containing 0.05 μg/ml anti-Mono-ADP-Ribose (Bio-Rad, AbD43647 #TZA020), which had been conjugated to BiCatcher2:HRP (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Protein samples were resolved using 4-12% Bis-Tris SDS-PAGE gel and stained with Coomassie Brilliant Blue (Bio-Rad Cat#1610436). Protein identity was confirmed by western blotting using anti-GST (1:5000 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples were loaded on 4–15% Criterion™ Tris-HCl Protein Gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Bio-Rad). Nonspecific binding sites were blocked for 1 h at room temperature in 5% nonfat milk in PBS-T ...
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bioRxiv - Cell Biology 2024Quote: Cell lysates and membrane fractions were resolved in home-made SDS-PAGE gels ranging from 8% to 18% acrylamide or using 4-20% gradient mini-PROTEAN TGX precast gels (BioRad, Mississauga, ON). Electrophoresis was done in Tris-glycine running buffer (25 mM Tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... Select Ni-NTA elution samples were run with a precast 4-20% Mini-PROTEAN® TGX Stain-Free Protein Gel (Bio-Rad), using a Precision Plus Protein Standard (Bio-Rad) ...
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bioRxiv - Physiology 2024Quote: ... 15 μL of samples were pipetted onto SDS gels (4-15% Criterion TGX Stain-free gels, Bio-Rad Laboratories; Hercules, CA, USA), and proteins were separated by electrophoresis at 200 V for 45-50 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and 30 µg of each sample were loaded and run on Mini-PROTEAN TGX 4-15% acrylamide gels (Bio-Rad, Hercules, CA). Proteins were transferred to PVDF membranes ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction mixture was then passed through a P6 Micro Bio-Spin chromatography column (Bio-Rad, 1,000 x g, 4 min, rt), to give the labelled oligonucleotide at ∼500 nM ...
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bioRxiv - Cell Biology 2024Quote: ... and NuMA MO with GFP-NuMA were lysed and fractionated by SDS-PAGE on 4-15% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-rad, 4568083) at 120 V ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction was incubated at room temperature for 30 minutes in the dark and then passed through a Biogel P-4 column (Bio-Rad Inc.) equilibrated with buffer (20 mM Tris-Cl ...
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bioRxiv - Cell Biology 2024Quote: ... Samples containing 30 µg protein were then loaded into Criterion TGX Precast 12+12 well comb 4-15% polyacrylamide gels (Bio-Rad, #5671083). Precision Plus Protein Kaleidoscope Ladder (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... were loaded and run on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Cat.: 4561094, Lot.: 64360183, Bio-Rad, USA) at a constant voltage of 120V for 65 min in a Mini-PROTEAN Tetra Cell (Cat. ...
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bioRxiv - Cell Biology 2024Quote: IP eluates and inputs (saved at beginning of stage iii) were loaded on 4–12% Criterion XT Bis-Tris precast protein gels (Bio-Rad #3450124), run in NuPAGE MOPS SDS running buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative PCR (qPCR) was performed with primer sets corresponding to primer list (Table 3) and using iTaq Universal SYBR Green Supermix (Bio-Rad, Cat. No. 172-5125) on an Applied Biosystems 7500 Fast Real-Time PCR system or ThermoFisher Scientific QuantStudio 3 or 5 Real-Time PCR system ...
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bioRxiv - Biochemistry 2022Quote: ... The plate was centrifuged at 2000rpm (3 minutes at room temperature) and placed in the CFX Touch™ Real Time PCR Detection System (Bio-Rad; Hercules, California, USA) set for an initial denaturation (95°C ...
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bioRxiv - Plant Biology 2022Quote: ... Amplification of target amplicons with the addition of 5’ XbaI and 3’ BamHI restriction enzyme digestion sites was carried out on a Bio-Rad T100 thermocycler (Bio-Rad Laboratories, Hercules CA, USA) via Bio-Rad iProof High Fidelity DNA Polymerase using manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots of clarified lysate (3 x 106 parasite equivalents) were resolved by SDS-PAGE (Mini-PROTEAN®TGX protein gel; Bio-Rad, Hercules, California, USA), transferred to PVDF membrane (Immobilon®-FL ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was washed again with 1x PBS-T (0.075 %) 3 times 10 minutes each and incubated with 1:1 ECL chemiluminescence solution (Clarity Western ECL, #170-5061, Bio-Rad Laboratories, Hercules, CA, USA). Signal was detected using an Amersham AI600 imager (Supplementary Table 5).
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bioRxiv - Microbiology 2024Quote: ... Then, 3 mL of each culture were pelleted (11,000 rpm, 1 min) to perform total RNA extraction (using BioRad’s Aurum™ Total RNA Mini Kit). RNA concentration and purity were assessed using a nanodrop and all samples shown to have A260/A280 and A260/A230 ratios ≥ 2 ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluted proteins were separated by NuPAGE 4-12% Bis-Tris gel in MES SDS running buffer and transferred to a PVDF membrane (Bio-Rad, 162-0263). Tubulin beta chain protein was detected with an anti-TUBB antibody (1:500 in 5% fat-free milk solution in TBST ...
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bioRxiv - Cell Biology 2020Quote: ... 40 μg of whole cell protein lysate were applied to 4%-15% Mini-PROTEAN TGX precast protein Gel (Bio-Rad, Cat No. 4561084) with electrophoresis and then transferred to a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2020Quote: ... followed by 1◻h incubation at 4◻°C with HRP-conjugated goat anti-mouse (1:5000 dilution in 1% YE in PBS-T, BioRad #170–6516) secondary antibody ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were separated on 6-10 % SDS-polyacrylamide gels or 4-20 % Mini-PROTEAN TGX precast gradient gels (Bio-Rad, CA, USA) together with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... The purity and integrity of the proteins was evaluated for each batch by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-15% gradient gels (Mini-PROTEAN® TGX® Precast Protein Gels, Bio-Rad) with a Precision Plus Protein® Kaleidoscope® (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Twenty micrograms of protein were loaded on 4-15% gradient TGX Stain-Free™ Protein Gels and transferred to Immun-Blot® LF PVDF membrane (Bio-Rad). The membranes were blocked with 1X TBS with 0.05% Tween 20 and 5% skim milk powder for 2 hours at 25°C ...
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bioRxiv - Microbiology 2020Quote: The samples used for immunoblot analysis were separated by SDS-PAGE on a 4-20% gradient gel (Mini-PROTEAN TGX Gels, Biorad, Cat# 456-1093). Gel electrophoresis was performed at 120 V in running buffer (Tris-glycine ...
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bioRxiv - Neuroscience 2021Quote: ... Around 10-15 μl (around 20 μg of protein) were resolved by SDS-PAGE polyacrylamide gel electrophoresis using 4-20 % precast gradient gels (Bio-Rad, 456-1096), and proteins were electrophoretically transfected onto nitrocellulose membranes (GE Healthcare) ...
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bioRxiv - Developmental Biology 2020Quote: ... and duck heads were fixed in 4% PFA overnight and stained for 20 minutes with 0.02% ethidium bromide (1610433, Bio-Rad, Hercules, CA, USA) using a previously published protocol (Eames and Schneider ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were resuspended in Laemmli buffer and loaded into 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Cat #4561093). SDS-PAGE and protein blotting were performed according to (Dawe et al ...
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bioRxiv - Molecular Biology 2021Quote: ... The boiled proteins were separated on 4% to 15% SDS-PAGE gradient gels and transferred to LF PVDF membrane (Bio-Rad, Cat #1620260). The membranes were blocked in 5% skim milk in TBST (20 mM-Tris ...
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bioRxiv - Cancer Biology 2020Quote: Proteins from WCL samples were separated on 4-20% (w/v) Tris-Glycine SDS-PAGE gels (Bio-Rad; Cat#456-1094, Hercules, CA). We loaded 30-50 μg protein per sample ...
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bioRxiv - Bioengineering 2020Quote: ... 1 µg of each protein sample was separated in one dimension using 4-20% tris-glycine gels (Mini-PROTEAN TGX, Bio-Rad Laboratories, Inc.). A 1:6 dilution of the PageRuler Plus pre-stained protein ladder (10 µl ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Samples were prepared as described in the specific experiment and then loaded on Criterion 4-15% Tris-HCl gels (BioRad 5678083 and 5678084) and separated at 200V ...
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bioRxiv - Neuroscience 2020Quote: ... were mixed with 4× Bio-Rad sample buffer and loaded onto 4%–15% Trisglycine sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gels (Bio-Rad, Hercules, CA). Proteins were transferred to supported nitrocellulose membrane and probed using Syn101 antibodies against aSyn (BD labs ...
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bioRxiv - Biochemistry 2021Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by SDS-PAGE (90 min at 150V) using 4-20% Criterion™ TGX Stain-Free™ gel (Bio-Rad, 5678095) and electro-blotted onto nitrocellulose membranes (Bio-Rad ...