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Citations for Bio-Rad :
4501 - 4550 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... The rabbit polyclonal antiserum was raised against purified recombinant Thp1 protein and subsequently affinity purified as Thp1p coated affinity beads (Affi-Gel 10 beads, Bio-Rad) according to standard procedures ...
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bioRxiv - Molecular Biology 2023Quote: ... 40 μg of protein extract was loaded into a 10% TGX Stain-Free FastCast Acrylamide Gel (Bio-Rad, Hercules, CA, USA) or SDS polyacrylamide 15% running gel in the case of histone analysis ...
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bioRxiv - Pathology 2023Quote: ... Each individual and multiplexed TaqMan reaction was performed in a final volume of 10 µL containing 5 µL of Sso Advanced Universal Probes Supermix (Bio-Rad), 600 nM of each primer ...
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bioRxiv - Immunology 2023Quote: ... equal amounts of protein (10–40 μg) were loaded into 10-20% SDS–PAGE gradient gels and transferred onto 0.22 μM PVDF membranes (Bio-Rad, 1620177). After air drying to return to a hydrophobic state ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were denatured by boiling for 10 minutes in the DTT containing sample buffer and separated by 4-20% Mini-PROTEIN TGX gels (BIO-RAD) and then transferred to the 0.2 μm PVDF membrane (BIO-RAD) ...
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bioRxiv - Cell Biology 2023Quote: ... Extracts were run on 10% SDS-PAGE and transferred to a polyvinylidene difluoride membrane using a transfer apparatus following the standard protocols (Bio-Rad). After incubation with 5% nonfat milk in TBST (10 mM Tris ...
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bioRxiv - Cancer Biology 2023Quote: ... The reaction was stopped by 10% acetic acid and the gel was washed with water for visualization by ChemiDoc Imaging system (Bio-Rad).
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bioRxiv - Immunology 2023Quote: ... Even amounts of each protein sample were separated on a 8–10% polyacrylamide gel using Mini-PROTEAN Tetra Cell electrophoresis system (Bio-Rad). Upon completion of electrophoresis ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 µL of the bisulfite converted DNA was combined with 10 µL of Precision Melt Supermix for High Resolution Melt Analysis (BioRad 1725112), 2 µL of Forward and Reverse primers (2 µM stock ...
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bioRxiv - Microbiology 2023Quote: Total protein extracts (5x106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVDF Membranes (Bio-Rad). Immunodetection was performed using the primary antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... 1x DreamTaq buffer and 1 μL sample DNA in a total reaction volume of 10 μL using CFX96 Touch Real-Time PCR Detection System (BIO-RAD). The PCR amplification cycle was as follows ...
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bioRxiv - Genomics 2023Quote: ... RT-PCR was performed in triplicates and carried out in 20 µl of reaction mixture containing 10 µl of Fast SYBR® Green Master Mix (Biorad), 0.2 µl (10 pmol/µl ...
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bioRxiv - Cell Biology 2023Quote: ... The total cDNA obtained was diluted 1:10 and 2 µl was used for qRT-PCR by using gene specific primer and SYBR green PCR mix (BIO-RAD) on BIO-RAD CFX96 real time PCR machine ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately equal loads of extracted protein was then resolved on tris-glycine 10% SDS-PAGE gels and transferred using wet transfer to 0.2 µm nitrocellulose membrane (Bio-Rad 1620112). Transferred proteins were then stained with Ponceau S Solution (Sigma-Aldrich P3504 ...
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bioRxiv - Molecular Biology 2023Quote: ... Five micrograms of total protein were resolved by SDS-PAGE on a 10% gel under reducing conditions and transferred to PVDF membranes (Bio-Rad). The blots were blocked in TBS containing 5% non-fat milk for 30 mins and incubated with SARS-CoV-2 nucleocapsid protein antibody (1:1000 ...
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bioRxiv - Physiology 2023Quote: ... The protein extract (40μg) was separated on 8 or 10% polyacrylamide mini gel and transferred to a PVDF membrane (Bio-Rad, USA). Blotted membranes were incubated overnight at 4°C in SuperBlock(PBS ...
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bioRxiv - Microbiology 2023Quote: ... Total protein was transferred from a 10% SDS-PAGE gel onto a nitrocellulose membrane using the Trans-Blot turbo transfer system (Bio-Rad). Total Flag-sakA was detected using the Flag M2 antibody 1:2000 (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein lysates were run on 10% sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) and transferred to a nitrocellulose membrane (Bio-Rad) using a wet tank blotting system ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 μg of total protein was resolved on 10% SDS-PAGE gels followed by immunoblotting on PVDF membranes (Bio-rad). Membranes were developed by chemiluminescence (SuperSignal West Pico ...
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bioRxiv - Biophysics 2022Quote: ... Gels were fixed for 10min (50% methanol, 10% acetic acid) and stained with Coomassie Blue for imaging on a gel doc (Bio-Rad). Band intensities were quantified using Image Lab (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were subjected to 10–12% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Developmental Biology 2023Quote: ... 0.5% Triton X-10 in Sterile MilliQ water) and resolved on 10% SDS-PAGE gels followed by western blotting on Immun-Blot PVDF membranes (Bio-rad). Antibodies used were diluted in 5% milk (Labscientific CN ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were incubated at 95°C for 5 min and 10 μL of the sample was resolved on a 4%–15% Mini-PROTEAN® TGX Precast Gel (Biorad) and stained with Coomassie Blue.
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bioRxiv - Biochemistry 2023Quote: ... The samples were incubated at 95°C for 5 min and 10 μL of the sample was resolved on a 4%–15% Mini-PROTEAN® TGX Precast Gel (Biorad) and stained with Coomassie blue dye.
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bioRxiv - Plant Biology 2023Quote: Protein samples of whole cell extracts (0.5 µg chlorophyll or 10 µg protein) were loaded on 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and blotted onto nitrocellulose membranes ...
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bioRxiv - Plant Biology 2023Quote: ... and 10 (V/V) % beta-mercaptoethanol for 5 minutes before being loaded into a 4-20% gradient SDS-PAGE gel (Biorad: 4561096) and run according the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were boiled for 8–10 min at 95°C and loaded onto precast 12% Bis-Tris gels (Bio-Rad) with MES SDS running buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... The concentrated protein solution was loaded into a HiLoad 16/600 Superdex 200 pg column (Cytiva) connected to an NGC Quest 10 Plus system (Bio-rad) placed in a 4 °C chamber ...
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bioRxiv - Genomics 2023Quote: ... and 500 nM each primer up to a total volume of 10 μl and amplified in a CFX96 Real-Time System (Bio-Rad) with the following program ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were run with 10 mM H2SO4 at 0.6 mL/min on an Aminex HPX-87H (Biorad Inc., CA, USA) column at 50℃ ...
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bioRxiv - Cell Biology 2023Quote: 5 to 10 ng of cDNA were used to perform quantitative polymerase chain reaction using 1X SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). All RT-qPCR reactions were performed in a CFX Connect Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-15 ug of protein lysates were run on 4– 20% Criterion™ TGX™ Precast Midi Protein Gel (Bio-Rad) and then transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Plant Biology 2023Quote: ... extracted proteins were diluted in SDS loading dye and incubated at 72°C for 10 min and were loaded to 4-20% Mini-PROTEAN TGX gels (Bio-Rad). Immunoblotting was performed with antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were denatured by boiling for 10 min in the DTT-containing sample buffer and separated by 4–20% Mini-PROTEIN TGX gels (Bio-Rad) and then transferred to the 0.2 µm polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2023Quote: Whole cell lysates and eluates from immunoprecipitations were run on 10% SDS-polyacrylamide gels and transferred to supported nitrocellulose membrane (Bio-Rad) by standard methods ...
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bioRxiv - Microbiology 2023Quote: Total protein extracts (5×106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVDF Membranes (Bio-Rad). Immunodetection was performed using the primary antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... The HURP antibody was then purified by first conjugating 10 mg of HURP(462-576) antigen to 0.5 mL of Affi-gel 15 (Bio-Rad: 1536051) resin (brought up to 10 mL with PBS buffer) ...
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bioRxiv - Molecular Biology 2023Quote: qPCR was run with cDNA previously generated from rescue experiments in a 10 µl reaction using iTaq Universal SYBR Green Master Mix (BioRad 1725121) with readout using the Applied Biosystems ViiA 7 real-time PCR system with standard cycling parameters (50°C for 1 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR (qPCR) was performed in a 10 µL reaction that contained 5 µL SsoAdvanced™ Universal SYBR Green Supermix (BioRad), 1 µL H2O ...
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bioRxiv - Neuroscience 2023Quote: ... 10 ug of protein from cortical samples and 45ug of muscle samples were diluted in 4x Laemmli sample buffer (Bio-Rad). P3 brain samples were already directly resuspended in 4x Laemmli sample buffer ...
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bioRxiv - Biophysics 2023Quote: ... samples were processed using a Bio-Rad NGC Quest 10 Plus fast protein liquid chromatography (FPLC) apparatus with a 5 mL EconoFit Nuvia IMAC affinity column (Bio-Rad). To purify protein from the soluble fraction ...
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bioRxiv - Biophysics 2023Quote: ... UBQLN1 D15R and UBQLN1 E21Q were subjected to size exclusion chromatography over an ENrich™ SEC 650 10 x 300 column (BioRad) in 20 mM sodium phosphate buffer pH 6.8 ...
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bioRxiv - Biochemistry 2023Quote: Analytical size exclusion chromatography was carried out on a Waters HPLC with an ENrichTM SEC 650 10 x 300 column (Bio-Rad) in 20 mM HEPES pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... 3 μl ten-fold diluted cDNA preparation was mixed in a final volume of 10 μl containing 5 μl iQ SYBR Green Supermix (Bio-Rad) and 4 pmol of each primer (Fw ...
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bioRxiv - Cancer Biology 2023Quote: The prepared lysates were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto PVDF membranes (Bio-Rad, USA). The membranes were then blocked and incubated overnight at 4°C with the primary antibodies (as listed in Table S6) ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μg of each sample was boiled with 4x Laemmli Sample Buffer containing 10% 2-mercaptoethanol (#1610747, BioRad, Hercules, CA, USA) and run on a Mini-PROTEAN® TGX™ Precast Gel (#4561083 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were boiled for 10 min at 94°C before loading on polyacrylamide gels (4-15% Bis-Tris Gel, Bio-Rad). Proteins were separated by gel electrophoreses at 80 V and transferred at 90 V for 2 h on nitrocellulose membranes (Precast Protein Gels ...
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bioRxiv - Microbiology 2023Quote: ... Antibiotic disk content used in the assays were cefotaxime 30 μg and amoxicillin + clavulanic acid 30 μg (20 μg + 10 μg; all from Bio-Rad).
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bioRxiv - Immunology 2023Quote: ... 10 µg of RNA was transfected into 4×10^6 S10-3 cells by electroporation with a Gene Pulser II apparatus (Bio-Rad) in 0.4 cm Gene Pulser cuvettes (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 µg of total protein was resolved on 10% SDS-PAGE gels followed by immunoblotting on PVDF membranes (Bio-rad). Membranes were developed by chemiluminescence (SuperSignal West Pico ...