Labshake search
Citations for Bio-Rad :
4401 - 4450 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2020Quote: ... coli cells were prepared using 10% glycerol (Applichem) and transformed with the corresponding plasmids using a “MicroPulser” electroporator (BioRad, Hercules, US) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Enzymatic biotinylation with BirA ligase was performed and protein was purified by size-exclusion fast protein liquid chromatography using a NGC Quest 10 Chromatography System (Bio-Rad).
-
bioRxiv - Immunology 2021Quote: ... P2 was dialyzed against 1% glycine containing 2% ampholytes pH 3-10 (first run) or pH 5-7 (second run) and then loaded onto a liquid isoelectric focusing system (Rotofor, Bio-Rad). Individual fractions were harvested and their pH and absorbance at 280nm were measured ...
-
bioRxiv - Immunology 2021Quote: ... pooled and subjected to two consecutive preparative isoelectric focusing (IEF) at pH 3-10 (first separation) and pH 5-7 (second separation) using a Rotofor Cell (Bio-Rad). The individual IEF fractions were harvested ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 15 μg of lysate was mixed with a 5x loading buffer and separated by 10% SDS-PAGE before being transferred to a nitrocellulose membrane (Bio-Rad).
-
bioRxiv - Physiology 2019Quote: ... The total protein (about 50 μg) was separated in an SDS-PAGE (10 %) and transferred onto nitrocellulose membrane (Bio-Rad, America). The membranes were blocked with TBST (137 mM NaCl ...
-
bioRxiv - Cancer Biology 2020Quote: ... The beads were washed five times with IP lysis buffer and were boiled for 5-10 min in 2 times Laemmli sample buffer (#1610737, Bio-Rad) with 2-mercaptoethanol (#BP176-100 ...
-
bioRxiv - Molecular Biology 2019Quote: ... Protein separation was done on 10% SDS-PAGE gel electrophoresis before being blotted on a nitrocellulose membrane (Trans-Blot turbo, Bio-Rad). The membrane was blocked with 5% non-fat dry milk in TBST (TBS buffer containing 0.05% tween 20 ...
-
bioRxiv - Neuroscience 2021Quote: ... 10-50 μg of protein was loaded onto 4-15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad) and transferred to polyvinylidene difluoride (PVDF ...
-
bioRxiv - Cell Biology 2020Quote: Transfected cells were lysed in RIPA buffer on day 4 and proteins extracted were separated by 10% - 15% SDS polyacrylamide gel and transferred to Nitrocellulose membranes (Bio-Rad) at 100V for 1 hour and blocked for 1 hour with 5% non-fat milk (Bio-Rad ...
-
bioRxiv - Microbiology 2020Quote: ... PCRs were prepared in a total volume of 20 μl containing 10 μl of iQ SYBR Green Supermix (Bio-Rad, USA), 0.5 μM each primer and 100 ng of cDNA ...
-
bioRxiv - Immunology 2021Quote: ... The proteins were fractionated on a 10% sodium dodecyl sulfate–polyacrylamide gel and transferred to polyvinylidene difluoride (PVDF) membrane using a semi dry Transfer Cell (Bio-Rad), which was run at 18 V for 1h ...
-
bioRxiv - Biochemistry 2021Quote: Polymerizing gel solution was made by combining 15 mL of 9.5% gel solution with 133 uL of 10% Ammonium Persulfate Solution (APS) and 13.3uL TEMED (BIO-RAD #161-0700) into a beaker under a fume hood ...
-
bioRxiv - Biochemistry 2021Quote: Equal amount of protein was loaded and resolved on 10% polyacrylamide gel and transferred onto the PVDF membrane supplied by Bio-Rad, USA ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins (20 μg – 40 μg depending on the target protein) were resolved by 10%-12% SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (BioRad, USA). The membranes were blocked with 5% non-fat milk for 2 h at RT and subsequently probed with specific primary antibodies overnight at 4°C (Table EV2) ...
-
bioRxiv - Cancer Biology 2021Quote: ... the equal quantity of protein (40 µg) was resolved on 10 % polyacrylamide gel and transferred onto the PVDF membrane supplied by Bio-Rad, USA ...
-
bioRxiv - Immunology 2022Quote: ... was centrifuged at 500 x g for 10 min Protein levels in the supernatant were measured by Bradford assay (Bio-Rad), and cytokine levels were measured using a custom multiplex ELISA (ThermoFisher Scientific).
-
bioRxiv - Biochemistry 2020Quote: ... was applied to Sephadex G-10 column (85 ml, fractionation range <700 Da) fitted with Biologic duo flow instrument (Bio-Rad) at a flow rate of 6 ml/hr ...
-
bioRxiv - Microbiology 2021Quote: ... Expression and secretion samples were incubated at 95 °C for 10 minutes and then resolved on TGX Stain-free gel (Bio-Rad). Next ...
-
bioRxiv - Genomics 2020Quote: ... heated at 95°C for 10 min and run on a Mini-protean TGX 4%-20% polyacrylamide gel (Biorad, 456-1095). The proteins were transferred onto PVDF membranes using the Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad ...
-
bioRxiv - Microbiology 2020Quote: ... The plate was incubated 10 min at room temperature to digest the target DNA before droplets were generated in an Automated Droplet Generator (Bio-Rad). PCR was performed with a hot-start/enzyme activation at 95°C for 5 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... whole cell lysates were incubated at 100°C for 10 minutes in 50 μl SDS loading buffer (950 μl Bio-Rad® Laemmli buffer ...
-
bioRxiv - Neuroscience 2021Quote: ... 10 µg of protein per sample were boiled for 5 min and size separated by electrophoresis in polyacrylamide gels (Bio-Rad). Proteins were transferred over-night at 4 °C to nitrocellulose membranes (Bio-Rad ...
-
bioRxiv - Microbiology 2020Quote: ... Optimized ddPCR was used to detect the presence of SARS-CoV-2 viruses following our previous study.10 Analysis of the ddPCR data was performed with QuantaSoft software (Bio-Rad). The concentration reported by the procedure equals copies of template per microliter of the final 1x ddPCR reaction ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins from lysates were separated by electrophoresis in a sodium dodecyl sulfate (SDS)-10% polyacrylamide gel (SDS-PAGE; Bio-Rad, Munich) and subsequently transferred to a nitrocellulose membrane by electroblotting ...
-
bioRxiv - Immunology 2021Quote: ... Cell lysates or lung homogenates in denaturing sample buffer (10-20 µg total protein) were separated by SDS-PAGE on 4-15% Mini Protean TGX gels (Bio-Rad) and transferred electrophoretically to a 0.45 µm nitrocellulose blotting membrane (GE Healthcare ...
-
bioRxiv - Immunology 2020Quote: ... Purified products were analyzed by 10% TBE PAGE and total protein quantified with Bio-Rad protein quantification reagent (Bio-Rad, 5000006). LPS contamination after conjugation was below 0.5 EU/mg as mentioned below in the vaccinations section.
-
bioRxiv - Immunology 2021Quote: ... Beads were pelleted and 10 µL of the sample supernatant was run on a 10% SDS-PAGE gel and subsequently transferred to a PVDF membrane (Trans-Blot Turbo Transfer System, Bio-Rad). Membranes were blocked with 4% dried skim milk (ELK ...
-
bioRxiv - Biochemistry 2020Quote: ... the buffer of the purified DNAJB6 was exchanged into 10 mM ammonium acetate pH 7 solution using Micro Biospin P6 centrifuge columns (Bio-Rad) and protein concentration determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... 50 μl of eluted samples were electrophoresed for 8 min at 200 V on a 10% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and protein was stained using Quick Blue Stain ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins (typically 10 to 20 μg) were separated by SDS-PAGE and transferred to PVDF using a wet transfer apparatus (Bio-Rad). Total protein was detected by staining with PonceauS (MilliporeSigma ...
-
bioRxiv - Neuroscience 2021Quote: ... Gels were run for 2 hours and 10 min at 80V and transferred using the Trans-Blot SD Semi-Dry Transfer cell system (Bio-Rad). After transfer the LI-COR Revert 700 Total Protein Stain (LI-COR ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein was loaded (10-50 µg per well, depending on protein of interest) and transferred using semi-dry turbo blotter (Bio-Rad). Blots were placed into 5% blocking solution containing primary antibodies and were incubated overnight at 4°C (Table 2) ...
-
bioRxiv - Neuroscience 2021Quote: ... Lysates containing approximately 40 μg of protein were boiled for 10 min prior to separation by 4-20% SDS-PAGE (BIO-RAD). After transferring at 90V to a nitrocellulose membrane for 90 min at room temperature ...
-
bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of proteins were separated on either 10% SDS/PAGE gels or 4-20% Mini-PROTEAN TGX Stain-Free Gels (#4568094, Bio-rad). Proteins were then transferred onto PVDF membranes and probed with the following antibodies ...
-
bioRxiv - Neuroscience 2022Quote: Samples from m7GTP pull-down assays and lysates were boiled at 70°C for 10 min in Laemmli sample buffer (Bio-Rad) and resolved in 10% or 4-15% gradient SDS-PAGE gels ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins (10μl solubilized in 2X Laemmli/20% of 2-mercaptoethanol) were separated by SDS-PAGE on 10% polyacrylamide gel (Bio-Rad, Hercules). Subsequently ...
-
bioRxiv - Genetics 2022Quote: ... Protein samples were heated at 37°C for 5 min and 10 μl was loaded on SDS–polyacrylamide gel electrophoresis (PAGE) gels (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad). After electrophoresis for 30 min at 200V ...
-
bioRxiv - Biochemistry 2022Quote: ... was measured over temperatures ranging from 10 °C to 95 °C using a CFX Connect Real-Time PCR Detection System (Bio-Rad). Melting temperature (Tm ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein concentration was measured on Tecan (Schoeller) and approximately 20 μg of protein lysate was loaded on the 10-well protein gels (Mini-PROTEAN TGX, Bio-Rad) and run at 120 V ...
-
bioRxiv - Biochemistry 2022Quote: ... Zero-minute timepoints were collected by independently adding 5 µL of each mixture to 10 µL 2X Laemmli Buffer (Bio-Rad) and boiling at 95 °C for 5 minutes ...
-
bioRxiv - Bioengineering 2022Quote: The purified baculoviruses were electrophoresed on 10% sodium dodecyl sulfate-polyacrylamide gel (BIOTOOLS) and transferred to a low-fluorescence polyvinylidene fluoride (PVDF) membrane (Bio-Rad) through Trans-Blot Turbo Transfer System (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... Ten to 30 μg of each sample were loaded onto 10% SDS-PAGE gels (TGX Stain-Free FastCast system, Bio-Rad), and ran at 230 V ...
-
bioRxiv - Developmental Biology 2022Quote: ... All samples were boiled with 1X Laemmli buffer containing 10% ß-mercaptoethanol before SDS-PAGE using Mini-PROTEAN TGX (Bio-Rad) pre-cast gels ...
-
bioRxiv - Cell Biology 2022Quote: ... Total protein was resolved on 10% SDS–polyacrylamide gel electrophoresis gels and transferred onto PVDF membranes (Bio-Rad, catalog no. 1704156) by Trans-Blot Turbo (Bio-rad) ...
-
bioRxiv - Genomics 2022Quote: ... and 20 ug of each sample were loaded on a 6% or 10% SDS-PAGE gel and transferred to a nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad). Western Blot was blocked using 5% ELK and incubated overnight with primary antibody mouse monoclonal anti-UHRF1 (Santa Cruz ...
-
bioRxiv - Genetics 2022Quote: ... All samples were incubated for 10 min at room temperature in 96 well plates before imaging on a Gel doc system (Bio-Rad). The optical density (OD600 ...
-
bioRxiv - Plant Biology 2022Quote: ... 0.01% [v/v] β-mercaptoethanol) was separated by 10% SDS-PAGE gel and blotted onto Immun-Blot PVDF membrane (Bio-Rad). D1 ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins were separated by SDS-PAGE on 10% or 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... denatured for 10 min at 95 °C and loaded on a 10% SDS-polyacrylamide gel (SDS-PAGE) and separated for 45 min at 200 V (Bio-Rad). Samples were transferred to a nitrocellulose membrane (TransBlot Turbo ...