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Citations for Bio-Rad :
4301 - 4350 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: 10-40 µg of denatured protein extracts were separated by SDS-PAGE using 10% acrylamide gels or 4-15% Mini-Protean precast gels (Bio-Rad) for 2 h at 120 V and transferred onto methanol-activated PVDF membranes at 350 mA for 2 h on ice ...
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bioRxiv - Molecular Biology 2021Quote: Samples in 1x Laemmli loading buffer (generally equivalent to 1 µL replication reaction or chromatin recovered from 8 µL plasmid pull-down) were resolved on 10% or 4-15% acrylamide Mini-PROTEAN or Criterion TGX precast gels (Bio-Rad) and transferred to PVDF membranes (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2020Quote: ... Extracts were then incubated on ice for 10 min before protein concentration was calculated by Bradford assay (Bio-Rad, 500-0006). Extracts were then mixed with Laemmli buffer and boiled at 95°C for 5 minutes before loading on SDS-PAGE gels.
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bioRxiv - Neuroscience 2021Quote: ... The samples were then separated on a 10% SDS-PAGE gel and transferred onto a nitrocellulose membrane (Bio-Rad, 162-0112). The membranes were incubated in a blocking solution (5% milk in TBS-T ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was mixed with 5× RT Reaction Buffer and 10× RT Enzyme Mix and transferred to a thermal cycler (Bio-Rad). Cycling conditions were as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... loaded on SDS-polyacrylamide gels (8 to 10 %) and transferred to nitrocellulose membranes using Trans-blot turbo transfer system (Bio-Rad). Membranes were saturated for 1 h with TBST buffer (150 mM NaCl ...
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bioRxiv - Physiology 2019Quote: For serum fetuin-A analysis murine serum was separated by sodium dodecyl sulphate polyacrylamide gel elecrophoresis using mini gels (10% acrylamide, 5 × 8 × 0.1 cm3, BioRad, Hercules, USA). Protein transfer onto nitrocellulose membrane was by semi-dry electroblotting (Owl HEP-1 ...
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bioRxiv - Neuroscience 2019Quote: ... boiled for 1 min and an equivalent of ∼1.5 brains was immediately loaded onto a 10% acrylamide gel for SDS-PAGE at 80V (Mini-Protean Cell, BioRad, Hercules, CA). Separated proteins were blotted onto nitrocellulose membranes at 20 V for 6 minutes using an iBlot system (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: Home-made gels: The home-made Laemmli resolving gel (10 ml) contains 3 ml of 40% Acrylamide/Bis (cat#: 1610148, Bio-Rad), 2.5 ml of 1.5M Tris-HCl pH 8.8 (cat# ...
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bioRxiv - Neuroscience 2019Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min prior to loading into into a 4-15% CriterionTM TGXTM precast Midi protein gel (Bio-Rad) for SDS-PAGE followed by wet transfer to nitrocellulose membrane ...
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bioRxiv - Immunology 2019Quote: ... the live cell number was counted by the trypan blue dye exclusion method using the TC 10 Automated Cell Counter (Bio-Rad). All B cell hybridoma lines were cultivated in high-glucose Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Synthetic Biology 2019Quote: ... qPCR reactions were prepared in triplicate in a final volume of 10 μL using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad), 0.5-5 ng of cDNA and 400 nM primers ...
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bioRxiv - Plant Biology 2020Quote: ... of total protein for each sample were separated by SDS-PAGE (10% acrylamide gel) and transferred to a PVDF membrane (Bio-Rad). The membrane was blocked in TBST buffer containing 5% (w/v ...
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bioRxiv - Evolutionary Biology 2020Quote: DNA of parents and selfed offspring was extracted on 96-well PCR plates using a 10-% Chelex bead solution (Bio-Rad) adapted from Walsh et al ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were collected in 80 ml Laemmli buffer and 20 ml of solution was used for SDS-Page (10 % acrylamide precast mini gels, Bio-Rad). A Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Bioengineering 2020Quote: ... Equal amounts of protein lysates (10-20 μg total protein) were subjected to 4–20% SDS-PAGE (Bio-Rad, Hercules, CA). Western analysis was carried out with primary antibodies against phosphorylated Smad1/5 (p-Smad1/5) ...
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bioRxiv - Microbiology 2021Quote: ... 15 mg/mL enoxaparin sodium derived from porcine intestinal mucosa (Sanofi-Aventis U.S., Bridgewater, NJ) was applied to a Bio-Gel P-10 column (2.5X120 cm, Bio-Rad, Hercules CA) and eluted with 0.2 M NH4HCO3 at a flow rate of 14 ml/h ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was washed three times with PBS-T (15 min, RT) and chemiluminescence was detected after 10 min incubation with freshly prepared Clarity Western ECL Substrate (Bio-Rad) with a LAS-4000 gel scanning station (Fujitsu Life Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were subjected to westernblot analysis using precast polyacrylamide gels (10-20% Tris-HCl or 4-12% Bis-Tris, Bio-Rad). For immunoprecipitation (IP ...
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bioRxiv - Biophysics 2020Quote: ... Inputs (2 μl) and elutions (10 μl) samples were analyzed by 4-15% SDS-PAGE (MINI-PROTEAN TGX stain-free, Bio-Rad) and staining with Quick Coomassie (Generon ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were washed and boiled in sample buffer and resolved on 10% SDS-PAGE or gradient gels (4 – 20%, Bio-Rad) and electro-transferred to PDVF membrane ...
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bioRxiv - Cell Biology 2021Quote: ... samples were boiled for 5 min and proteins were resolved on 10% SDS polyacrylamide gels and blotted onto nitrocellulose membranes (Bio-Rad). Anti-rabbit Phospho-p44/42 MAPK antibody (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples for RT-qPCR were prepared in a final volume of 10 µl and analyzed using Applied Biosystems’s SYBR Green PCR master mix (Bio-Rad Laboratories) during 40 cycles of amplification ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amount of proteins from each lysate were subjected to polyacrylamide gel (4-10 %) electrophoresis (PAGE) in Mini-PROTEAN Tetra cell apparatus (Bio-Rad). PAGE separated proteins were blotted onto a PVDF membrane in a semi-dry Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... were separated in a 10% acrylamide gel by SDS-PAGE and transferred to a nitrocellulose membrane according to the manufacturers protocols (Bio-Rad). Following a 60min incubation in 5% non-fat milk in TBS-T the membrane was incubated with primary and secondary antibodies (Supplementary Tables 3 ...
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR was performed in a 10 μL reaction using iQ™ SYBR® Green Supermix (Bio-Rad, Hercules, CA, USA). Applied Biosystems 7300 Real Time PCR system (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: Primary screening for nanobody binding was achieved by a small-scale pulldown method in which Affi-gel 10 affinity resin (Bio-Rad) was bound to laforin and subsequently to each purified VHH clone34 ...
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bioRxiv - Cell Biology 2021Quote: ... 10 to 30μg of total protein were subjected to 8% or 10% SDS-PAGE gels and transferred electrophoretically onto PVDF membrane by a semi-dry blotting system (Bio-Rad). The membrane was blocked in 5% fat-free milk/Tris-buffered saline for 1 hour and incubated with primary antibodies at 4°C overnight followed by horseradish peroxidase conjugated secondary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... and strains IU8675 and IU8722 at various time after Cm treatment lysates were loaded on a 10% precast gradient SDS-PAGE gel (Bio-Rad), subjected to electrophoresis and transferred to a nitrocellulose membrane ...
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bioRxiv - Microbiology 2020Quote: ... and placed on ice for 2 min before loading on a 10% denaturing urea polyacrylamide gel in TBE buffer (Bio-Rad) next to an RNA marker (low range ssRNA ladder ...
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bioRxiv - Systems Biology 2020Quote: Full-length amplicon (145 bp) was purified by non-denaturing polyacrylamide gel electrophoresis using precast 10% acrylamide TBE gels (Bio-Rad). The guide expression vector pRDA_052 was digested with BsmBI (New England Biolabs) ...
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bioRxiv - Neuroscience 2020Quote: ... SDS-Page was performed to resolve protein by molecular weight using 10 micrograms of hippocampal lysate from each sample in 4-20% acrylamide pre-cast gels (Biorad, 4568094). Resolved protein was transferred to PVDF membrane and blocked with 5% BSA solution in PBST ...
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bioRxiv - Genetics 2021Quote: ... The 13 μl of protein was run on a 10% SDS-PAGE gel and transferred to a PDVF membrane by semidry transfer (Bio-Rad) at 13V for 90 min ...
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bioRxiv - Microbiology 2021Quote: The eluted IP samples were separated by molecular weight using SDS-PAGE (precast 4-20% Mini-PROTEAN TGX Stain-Free Protein Gels, 10 well, 50 μl; Bio-Rad) at 160 V for 10 min ...
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bioRxiv - Physiology 2021Quote: ... Protein aliquots were denatured in Laemmli sample buffer at 100°C for 5 min and then loaded (10– 15 μg protein per lane) on 4–20% Criterion TGX Stain-free gels (Bio-Rad) and run for 45 min at 200 V ...
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bioRxiv - Physiology 2020Quote: ... 15 μg of protein was separated on a 10% Criterion® TGX™ Precast Protein Gel (BioRad Laboratories, 56671034, Hercules, CA) and transferred to a nitrocellulose membrane using a Trans-Blot TurboTransfer System (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2020Quote: ... or immunoprecipitation products were denatured by boiling for 10 minutes and run on a 4-12% Gradient Bis-Tris Gel (Bio-Rad) before being transferred to a PVDF membrane (Millipore) ...
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bioRxiv - Microbiology 2019Quote: ... samples were boiled for 10 minutes and loaded on 4–20% Mini-PROTEAN TGX gradient gels (Bio-Rad Laboratories, Hercules, CA).
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bioRxiv - Microbiology 2019Quote: ... PCR was performed on 10 ng of each pooled DNA sample using a PTC-200 thermal cycler (Peltier, BioRad, Hercules, USA). Amplification started with an initial denaturation step of 2 min at 94°C ...
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bioRxiv - Cancer Biology 2020Quote: ... 30–40 µg of total protein were loading into each well of a 10% SDS-PAGE gel and separated using an electroporation system (Bio-Rad). Proteins were transferred onto an NC membrane (Whatman) ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μg of proteins were then separated on a 4-15% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad, Ref: 4568085) at 160V ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated on a polyacrylamide gel with 10% of sodium dodecyl sulfate(SDS) (Mini Protean III; Bio-Rad, Richmond, CA), and they were transferred for 2 hours at 48°C to nitrocellulose membranes of 0.45 μm ...
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bioRxiv - Cell Biology 2020Quote: ... Triton-insoluble material was then pelleted at 15890 x g for 10 min and protein concentration measured with the DC assay (Bio-rad). For immunoblot analysis ...
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bioRxiv - Cell Biology 2021Quote: ... 100 µg of protein were loaded per lane and electrophoresed on a 10% TGX precast SDS PAGE gel from Bio-Rad. Protein was transferred onto a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2022Quote: ... was mixed with 10 mM DTT and bromophenol blue and loaded into Any kD Criterion TGX Precast Gels (567-1124, Bio-Rad). PVDF membranes (170-4157 ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein samples (30-50 µg) or IP samples were resolved by electrophoresis on 10% or 12% Mini or Midi-TGX Precast Gels (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... SYPRO orange fluorescence data in relative fluorescence unit (RFU) was collected from 10 °C to 95 °C using CFX Connect Real-Time PCR Detection System (Bio-Rad). The temperature corresponding to the lowest point of the first derivative −d(RFU)/dT was determined to be the Tm.
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bioRxiv - Cell Biology 2022Quote: ... Standard qPCR reactions with 5 or 10 ng cDNA per reaction were performed with iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX Connect Real-Time PCR Detection System ...
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bioRxiv - Cell Biology 2022Quote: ... 20 ng of cDNA and 0.4 μM of each primer set was mixed in a 10 μl RT-PCR reaction that was ran on the CFX96 System (Bio-Rad). The primers that were used span exons ...
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bioRxiv - Biophysics 2022Quote: ... The labelled proteins were further purified and separated from free Alexa Fluor dye using a Superdex 75 10/300 GL gel filtration column (Cytiva) using either an ÄKTA protein purification system (Cytiva) or NGC Chromatography System (Bio-Rad). The proteins were concentrated using the Amicon Ultra centrifugal filters (Merck ...